The invention relates to application of trichocephalus microcoleus in production of GG and construction of engineered trichocephalus microcoleus with high yield of GG. According to the method disclosed by the invention, engineered
coleus microcoleus with high yield of GG is constructed, and culture conditions for synthesizing
glycerol glucoside by the
coleus microcoleus BL0902 are optimized. Glycerin and glucose are added into the culture medium at the same time, so that the GG yield is improved to the optimal degree, and the GG yield is improved by about 4 times. Through overexpression of gtr, ggpS and ggpP,
carbon flow is promoted to enter a path for synthesizing
glycerol glucoside. The result shows that the total GG yield of the gtr-overexpressed algal strain is increased from 193.6 mg / L to about 337 mg / L 24 hours after the gtr-overexpressed algal strain is externally added with
glycerol and glucose. On the basis, the ggpS and the ggpP are further overexpressed, and the total GG yield of the
algae strain is increased to about 984.7 mg / L. In conclusion, the
key genes ggpS and ggpP synthesized by overexpressing the
glucose transporter gene gtr and GG, the GG yield is remarkably increased and can reach 984.7 mg / L to the maximum, and the yield is 393.88 mg / L / d.