The invention discloses a method for detecting the resistance of polypogon fugax to ACCase inhibitor herbicides, which comprises the following steps: detecting the resistance of polypogon fugax to ACCase inhibitor herbicides, extracting the
genome DNA of a polypogon fugax
plant to be detected, carrying out PCR amplification on the
genome DNA by using a KASP primer group, detecting the
fluorescence signal of the PCR product, and determining the
genotype of the 1781st
nucleotide of the ACCase
gene. And if the
nucleotide at the site is T, judging that the polypogon fugax
plant has resistance to ACCase inhibitor herbicides. According to the method, the ACCase inhibitor
herbicide resistance of the polypogon fugax is accurately judged, the problems that a traditional whole
plant bioassay method is time-consuming and labor-consuming, and the
drug resistance mechanism is difficult to directly determine are effectively solved, a powerful
technical support is provided for early-stage
resistance monitoring and accurate prevention and control of farmland weeds, and the method is suitable for popularization and application. Grain loss caused by
drug resistance of weeds can be reduced, and agricultural production efficiency and
sustainable development are guaranteed.