A method for promoting the accumulation of flavonoids in leaves of ornamental crabapple

Abstract

The invention discloses a method for promoting the accumulation of flavonoid substances in the leaf tissue of the ornamental crabapple of the ever-colored purple-leaved species, comprising: using the ornamental crabapple leaf species as materials, designing melatonin with different concentration gradients to treat the ornamental crabapple tissue culture seedlings, The leaves treated with 200μg / l melatonin were selected according to the phenotype, and the color of the leaves was very obvious, and there was no obvious change in the color of leaves at high or low concentrations. Subsequent detection of ornamental crabapple treated with 200μg / l melatonin The content of flavonoids in the leaves, the transcription levels of key genes in the flavonoid metabolism pathway, and the transcription levels of genes related to the melatonin pathway, the results showed that the anthocyanin content was the highest in the ornamental crabapple treated with 200 μg / l melatonin . The invention provides a method for improving the atavism phenomenon of the ornamental crabapple due to the increase in the number of generations, and further provides a good theoretical basis for the genetic improvement of the color-leaved plants in the future.

Description

technical field [0001] The invention relates to a method applied in tissue culture of ornamental crabapple, in particular to a method for promoting accumulation of flavonoids in leaf tissue of ornamental crabapple. Background technique [0002] Color is an important external feature of many plants. However, different color modules can be observed in the leaf tissue of ornamental crabapple. The special leaf color type of ornamental crabapple also provides a good research material for the study of the color-forming mechanism of color-leaved plants, which will change the status quo of my country's ornamental crabapple resources being rootstock and sporadic cultivation for a long time, and expand the use of fruit trees as ornamental, medicinal and greening plants. Research content and application fields of tree species. At present, in the breeding process of ornamental crabapple seedlings, tissue culture is an important means of producing virus-free seedlings, but in the proces...

AI Technical Summary

Problems solved by technology

At present, in the breeding process of ornamental crabapple seedlings, tissue culture is an important means of producing virus-free seedlings, but in the process of tissue culture, it is easy to have species degeneration, leaf discoloration and other phenomena

However, in the process of tissue culture, there are few reports on the rapid promotion of the content of flavonoids in the leaf tissue, thereby promoting the color of ornamental crabapple leaves.

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