Method for promoting accumulation of flavonoid substances of ornamental Chinese flowering crabapple leaf tissues

Abstract

The invention discloses a method for promoting the accumulation of flavonoid substances of ornamental Chinese flowering crabapple leaf tissues. The method comprises the following steps: by adopting the ornamental Chinese flowering crabapple leaf variety as a material, designing melatonin of different concentration gradients to treat an ornamental Chinese flowering crabapple tissue culture seedling, selecting the leaves treated by 200 micrograms / l of melatonin according to phenotype, wherein the leaf is apparent in color, the color of the leaf treated by the melatonin with higher concentrationor lower concentration is not apparently changed, then detecting a content of the flavonoid substances in the ornamental Chinese flowering crabapple leaves treated by the 200 micrograms / l of melatoninas well as a transcriptional level of a key gene in the flavonoid metabolism way, and a transcriptional level of the melatonin way related gene, wherein a result shows that in the ornamental Chineseflowering crabapple treated with the 200 micrograms / l of melatonin, the content of anthocyanin is the highest. A method is provided for improving the reversion phenomenon due to the increase of the subculture times of the ornamental Chinese flowering crabapple, and a good theoretical foundation is further provided for the genetic improvement of the color-leafed plant in the future.

Description

technical field [0001] The invention relates to a method applied in tissue culture of ornamental crabapple, in particular to a method for promoting accumulation of flavonoids in leaf tissue of ornamental crabapple. Background technique [0002] Color is an important external feature of many plants. However, different color modules can be observed in the leaf tissue of ornamental crabapple. The special leaf color type of ornamental crabapple also provides a good research material for the study of the color-forming mechanism of color-leaved plants, which will change the status quo of my country's ornamental crabapple resources being rootstock and sporadic cultivation for a long time, and expand the use of fruit trees as ornamental, medicinal and greening plants. Research content and application fields of tree species. At present, in the breeding process of ornamental crabapple seedlings, tissue culture is an important means of producing virus-free seedlings, but in the proces...

AI Technical Summary

Problems solved by technology

At present, in the breeding process of ornamental crabapple seedlings, tissue culture is an important means of producing virus-free seedlings, but in the process of tissue culture, it is easy to have species degeneration, leaf discoloration and other phenomena

However, in the process of tissue culture, there are few reports on the rapid promotion of the content of flavonoids in the leaf tissue, thereby promoting the color of ornamental crabapple leaves.

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