49 results about "Phenotypic screening" patented technology

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention relates to a double-gene rare variation and disease relevance prediction model and an establishment method and application thereof, and belongs to the technical field of biological medicines.The establishment method of the double-gene rare variation and disease relevance prediction model comprises the following steps that S1, a sample library is screened; s2, performing quality control on whole exome sequencing data (WES); s3, performing phenotype screening; s4, performing grouping design; s5, carrying out PheWAS logistic regression analysis; s6, performing Firth logistic regression analysis and verification; and S7, carrying out double-gene feature analysis and double-gene pathogenicity relevance prediction. The method for analyzing the correlation between the rare double-gene variation and all disease phenotypes is designed for the first time, and a new method is provided for screening hereditary pathogenic factors of various diseases.

The invention discloses application of a KASP molecular marker in identification or auxiliary identification of soybean hundred-grain weight character, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker auxiliary breeding. According to the application of the KASP molecular marker in identification or auxiliary identification of the soybean hundred-grain weight character, the genotype of the KASP molecular marker is G or A, and the KASP molecular marker is located at the 7703700bp position of the No.6 chromosome of a soybean genome Wm82. A2. V1. The obtained KASP marker for regulating and controlling the hundred-grain weight character gene of the soybean is suitable for rapid screening and molecular marker-assisted breeding of the relative height of the hundred-grain weight of the soybean. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention belongs to the field of tilapia mossambica breeding, and discloses a rapid and efficient tilapia mossambica breeding method and system.The method comprises the steps that tilapia mossambica populations with excellent character potential are collected and screened, and a basic breeding population is established; molecular markers related to target characters are developed, and individuals carrying excellent genes are rapidly screened; a target gene is precisely regulated and controlled through a CRISPR / Cas9 technology, so that stable inheritance of excellent characters is accelerated; phenotypes such as growth performance, disease resistance and environment resistance are efficiently evaluated and screened; the excellent characters are fixed; and the performance of the new strain under the actual breeding condition is evaluated. The efficient and low-cost tilapia mossambica breeding method is constructed by integrating molecular marker, gene editing and phenotype screening technologies, the breeding efficiency and the commercialization value of a new strain are remarkably improved, and powerful technical support is provided for the aquaculture industry.

The invention discloses a KASP molecular marker primer combination for identifying soybean hundred-grain weight character and application of the KASP molecular marker primer combination, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. The invention discloses a KASP molecular marker primer combination for identifying the soybean hundred-grain weight character. The sequence of the KASP molecular marker primer combination is shown as SEQ ID NO.3-5. The invention provides a novel and simple molecular marker and an auxiliary selection method through the obtained KASP marker for regulating the hundred-grain weight character gene of the soybean, and is suitable for rapid screening of the relative height of the hundred-grain weight of the soybean and molecular marker auxiliary breeding. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The application relates to the technical field of molecular biology, and discloses a cold-tolerance identification method of Pennisetum based on a 65-core molecular marker set, a primer group and a gene chip and application thereof. The application collects core germplasm resources of the Pennisetum through a system, and constructs a GWAS population by using multi-environment (different altitudes) conditions, so that a wide genetic background and phenotype variation are fully covered, and a material foundation for genetic analysis of cold tolerance is laid. In the aspect of cold-tolerance phenotype identification, the application innovatively takes the chlorophyll content of leaves and the percentage of green leaves after natural low-temperature stress in autumn and winter fields as the cold-tolerance evaluation indexes, judges individuals with more than 40 excellent allelic variations as high-cold-tolerance varieties, and establishes a rapid and non-destructive phenotype screening method which can be implemented at the seedling stage.

The invention relates to the field of astragalus membranaceus breeding, and particularly discloses an astragalus membranaceus breeding method combining space mutagenesis with phenotype screening, which comprises the following steps: S1, seed pretreatment: space mutagenesis: carrying original seeds on a return satellite, exposing the original seeds on an orbit 200-400km away from the ground for 48-72 hours, and receiving a space radiation dose of 2-5Gy; disinfection treatment: soaking the mutagenized seeds in a 3-5% sodium hypochlorite solution for 15-20 minutes, and then washing the seeds with sterile water for 3 times; through the synergistic effect of space mutation and phenotype screening, the limitation that traditional mutation breeding is high in randomness is broken through, the directional screening efficiency of target characters is remarkably improved, and meanwhile stable transmission of excellent hereditary characters is guaranteed; through combination of multi-dimensional phenotypic indexes of root development, biomass accumulation and stress resistance, superior plants with high medicinal component enrichment capacity and wide adaptability are accurately locked, and the problem of comprehensive performance imbalance caused by single character screening is solved.

The invention discloses a KASP molecular marker for a soybean hundred-grain weight character and application of the KASP molecular marker, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. The KASP molecular marker for the soybean hundred-grain weight character is located at the 7354533bp position of the No.6 chromosome of a soybean genome Wm82. A2. V1, and the genotype of the KASP molecular marker is A or T. The invention provides a novel and simple molecular marker and an auxiliary selection method through the obtained KASP marker for regulating the hundred-grain weight character gene of the soybean, and is suitable for rapid screening of the relative height of the hundred-grain weight of the soybean and molecular marker auxiliary breeding. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The present application relates to the technical field of genetic engineering, in particular to a rice G1 gene mutant g1-1437, its molecular marker and application. The rice G1 gene mutant g1-1437 provided by the present application takes rice G1 gene as reference sequence, the nucleotide sequence of the rice G1 gene is shown as SEQ ID NO. 8; the gene mutant g1-1437 comprises a mutation site that base C at the 112th position is mutated into base T. The mutation causes the chaff homologous transformation of rice into glume shell substances, which can be used as a phenotype screening marker of transgenic rice. The present application further provides a molecular marker identification method of the mutant, which has a good application prospect in the transference of new germplasm.

The present invention relates to an InDel molecular marker, primer pair, kit, and application related to high benzyl alcohol primulosides content, belonging to the technical fields of plant molecular biology and genetic breeding. The sequence of the InDel molecular marker is shown in SEQ ID No. 1. The method of identifying high benzyl alcohol primulosides using the InDel molecular marker is simple to operate, rapid and efficient, and highly accurate. It can significantly shorten the tea tree breeding cycle and reduce the cost and complexity of traditional phenotypic screening. The method is suitable for large-scale screening of high-benzyl alcohol primulosides tea varieties, providing important technical support for improving tea aroma quality and selecting high-quality tea varieties.

The invention discloses a sugarcane high-sugar breeding method based on multi-modal data fusion, which comprises the following steps: constructing a sugarcane high-sugar parent germplasm resource library and a multi-modal phenotype database, constructing a sugarcane sugar content prediction model, performing phenotype screening and early prediction on filial generations, and collecting and planting sugarcane high-sugar parent materials with known sugar expression. The method comprises the following steps: establishing a core germplasm resource library, systematically measuring and recording multi-modal phenotypic data, including sugar traits and morphological growth traits, of each parent material in a plurality of key growth periods of sugarcane, and establishing a sugarcane sugar content prediction model by taking morphological growth traits data and physiological and biochemical traits data in different growth periods as characteristic variables; according to the method, pure phenotype data driving is completely based on directly measured phenotype data and environmental data, expensive genotyping or sequencing is not needed, the technical threshold and application cost are reduced, pure phenotype data driving is achieved, multi-modal data fusion is achieved, and prediction is accurate.

The invention discloses a method for creating a new winter wheat rapid breeding seed and application thereof, according to the method, a wheat specific germplasm SN0594 which does not need vernalization and is short in growth cycle is used as a parent and is applied to the creation process of a wheat character rapid stabilizing material, and the problems of long time, large loss, easy damage and the like in the winter wheat character stabilizing process can be solved. The method for rapidly creating the stable population by using the wheat SN0594 comprises the following steps: selecting a population parent, and hybridizing the population parent with the wheat germplasm SN0594; performing selfing generation adding in a culture room to obtain an F2 group; performing phenotype identification and screening on the F2 generation in a culture room; and performing culture room selfing generation acceleration to obtain a stable material. According to the method, the breeding frequency of the winter wheat in one year can be increased, a rapid and efficient method is provided for rapidly stabilizing the characters of the winter wheat, a test material with a short growth cycle is provided for indoor tests such as wheat genetic transformation, and the application range of the winter wheat in the indoor tests is widened.

The application discloses a KASP molecular marker primer combination for identifying a soybean hundred-grain weight trait and application thereof, and belongs to the technical field of molecular marker development and molecular marker assisted breeding of soybean agronomic traits.The KASP molecular marker primer combination for identifying the soybean hundred-grain weight trait provided by the application has the sequences shown in SEQ ID NO.3-5.A KASP marker of a soybean hundred-grain weight trait gene obtained by the application provides a novel and simple molecular marker and assisted selection method, and is suitable for rapid screening of soybean hundred-grain weight and molecular marker assisted breeding.The molecular marker can detect the genotypes of different hundred-grain weight materials in a natural population, and can also be used for molecular assisted selection breeding of different hundred-grain weight soybean materials, replaces large-scale phenotype screening, reduces field work load, and greatly improves screening accuracy and efficiency.

This invention discloses a method for wheat grain quality detection and functional gene analysis based on hyperspectral imaging technology, belonging to the field of agricultural testing technology. The method includes: extracting hyperspectral indices; measuring wheat grain quality indicators as manual measurements; assigning weight coefficients to each quality indicator to digitize the wheat quality phenotype; extracting characteristic wavelengths to establish predictive models for each indicator and wheat quality values; performing GWAS on hyperspectral indices and chemical indicators as phenotypic traits with genotypes to screen SNP loci, conduct co-location analysis, obtain candidate genes, and perform functional verification. This invention, through hyperspectral technology combined with chemometric modeling, is suitable for real-time monitoring during wheat quality breeding and processing; by combining spectral phenotype with GWAS gene mining, it overcomes the limitations of traditional quality detection that only serves phenotypic screening, forming a closed loop of "non-destructive detection - gene localization," providing targeted genes for wheat quality improvement.

This invention provides a type of yeast called *Saccharomyces clavatum*, characterized in that the *Saccharomyces clavatum* is: *Saccharomyces clavatum* (… Saccharomycopsis fibuligera AMCC 32248, preservation number CCTCC NO: M 20251908. Enzyme activity phenotypic screening, carbon source utilization analysis, and quantitative enzyme activity detection showed that this strain possesses highly efficient β-glucosidase activity; further analysis of key genes related to β-glucosidase... BGL1 Specific functional sequences were extracted and identified. Enzyme production conditions were optimized using response surface methodology, resulting in a significant increase in enzyme activity compared to before optimization. In a simulated fermentation system, this strain significantly promoted the production of specific acids, esters, aldehydes, alcohols, and other volatile flavor compounds. Its enzyme production characteristics and flavor regulation capabilities provide a new technical solution for addressing issues such as low hydrolysis efficiency of glycosides and insufficient flavor release in fermentation systems, showing potential applications in the food, brewing, feed, and pharmaceutical industries.

The application discloses a soybean rapid directional backcross transformation method and application, and belongs to the technical field of soybean breeding. The method takes 315 soybean cultivars covering different ecological zones as receptor parents, and takes glyphosate-resistant soybean Zhonghuang 6106 as a donor parent to perform soybean directional backcross transformation. In the hybridization stage, light, temperature, water and fertilizer, and hybridization technology are improved in four directions, so that the hybridization success rate is maximally improved, a large number of usable offspring are provided for the subsequent backcross process, and screening is performed on hybrid offspring and backcross offspring. The screening method is improved, phenotype screening, specific molecular marker screening and background recovery rate screening are combined, the screening efficiency is significantly improved, the workload is reduced, the breeding time is maximally shortened, and the breeding efficiency is improved. The whole backcross transformation process can be completed within 2 years, compared with 6 years of the traditional method, and significant progress is achieved.

The invention provides saccharomycopsis fibuligera, which is characterized in that the saccharomycopsis fibuligera is saccharomycopsis fibuligera AMCC 32248, and the preservation number of the saccharomycopsis fibuligera is CCTCC (China Center for Type Culture Collection) NO: M 20251908. Enzyme activity phenotype screening, carbon source utilization analysis and enzyme activity quantitative detection show that the strain has efficient beta-glucosidase activity; furthermore, the key gene BGL1 of the beta-glucosidase is called, and a specific functional sequence is excavated. The enzyme production condition is optimized through a response surface method, and the enzyme activity is remarkably improved compared with that before optimization. In a simulated fermentation system, the strain can remarkably promote generation of volatile flavor substances such as specific acids, esters, aldehydes and alcohols, and a new technical scheme is provided for solving the problems of low hydrolysis efficiency of glycoside substances, insufficient flavor release and the like in the fermentation system due to the enzyme production characteristic and flavor regulation and control capability of the strain. The method has application potential in the fields of food, brewing, feed, medicine and the like.

The invention relates to a method for breeding a rice blast-resistant and herbicide-resistant japonica glutinous rice variety by overcoming Pigm adverse effects. A glutinous rice variety which is widely popularized in production and has excellent comprehensive characters is selected as a receptor parent, a broad-spectrum rice blast-resistant gene Pigm and a herbicide-resistant gene ALS are screened under the assistance of molecular markers, and the rice blast resistance gene Pigm and the herbicide-resistant gene ALS are obtained through backcross, selfing, multiple cross and phenotype screening based on thousand seed weight. The thousand seed weight of the japonica glutinous rice material is 26.5 g or above, and the japonica glutinous rice material is polymerized with broad-spectrum rice blast-resistant and herbicide-resistant genes. The glutinous rice new strain bred by the method successfully integrates two key genes of broad-spectrum high rice blast resistance Pigm and imidazolone herbicide resistance ALS, and main agronomic characters such as thousand seed weight, maturing rate, plant and leaf morphology and the like are equivalent to or better than those of an excellent receptor parent.

This invention discloses a cultivation device and method for millet breeding, belonging to the field of agricultural breeding technology. The millet breeding cultivation device includes a greenhouse box with a built-in lighting system and a spray system, and further includes: a support plate fixed to both sides of the inner wall of the greenhouse box, a rotating ring rotatably mounted on the support plate, and a cultivation bed fixed to the rotating ring via a connecting plate; and an anti-lodging simulation mechanism. This invention, by setting up an anti-lodging simulation mechanism, enables the axial reciprocating motion of the pusher and the rotational motion of the cultivation bed to provide multi-angle, flexible mechanical stimulation to the plants, simulating natural wind to enhance lodging resistance, and simultaneously completing surface soil loosening. No additional power is required, achieving automated coordination of lodging resistance training, soil loosening, and dehumidification, thus improving breeding efficiency and the reliability of phenotypic screening.

The invention discloses a KASP molecular marker closely linked with soybean hundred-grain weight character and application of the KASP molecular marker, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. According to the KASP molecular marker closely linked with the soybean hundred-grain weight character, the genotype of the KASP molecular marker is G or A, and the KASP molecular marker is located at the 7469403bp position of the No.6 chromosome of a soybean genome Wm82. A2. V1. The invention provides a novel and simple molecular marker and an auxiliary selection method according to a KASP marker of a soybean hundred-grain weight character regulating gene, and the molecular marker and the auxiliary selection method are suitable for rapid screening of soybean hundred-grain weight relative height and molecular marker auxiliary breeding. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention discloses a KASP molecular marker related to the pod number character and the grain number character of a soybean single plant and application of the KASP molecular marker, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. The KASP molecular marker related to the pod number character and the grain number character of the soybean single plant is located at the 45649932 bp site of the No.19 chromosome of the Wm82. A4. V1 version of the soybean genome, and the genotype of the KASP molecular marker is A or T. The invention provides a novel and simple molecular marker and an auxiliary selection method, which are suitable for rapid screening of relative height of pod number and grain number of single soybean plant and molecular marker auxiliary breeding. The molecular marker can be used for detecting genotypes of materials with different single plant pod numbers and grain numbers in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different single plant pod numbers and grain numbers, replaces large-scale phenotypic screening, reduces the field workload, and remarkably improves the screening accuracy and efficiency.

The present application discloses a strain of Enterobacter mori against Aeromonas veronii and its application, which is classified and named as Enterobacter mori (SCH0241). Enterobacter mori SCH0241, preserved in China Center for Type Culture Collection on October 17, 2024, with a preservation number of CCTCC NO: M 20242236. The Enterobacter mori SCH0241 of the present application is derived from the intestine of a highly stress-resistant healthy hybrid crucian carp “Hefang crucian carp No. 2”, has good contact-dependent bacteriostatic activity against Aeromonas veronii, and can improve the ability of zebrafish to resist Aeromonas veronii infection. Through the construction of an insertion mutation library and phenotype screening, it is found that the gene cluster of T6SS-2 of SCH0241 plays a contact inhibition toxicity. The Enterobacter mori SCH0241 of the present application provides a new powerful tool for the biological control of fish diseases caused by Aeromonas veronii.

The invention relates to a method for excavating rice low-phosphorus adaptive genes by combining phenotypes and transcriptomics. The method comprises the following steps: performing phenotype screening under low-phosphorus stress; carrying out gene localization and cloning; sequencing and analyzing a transcriptome; performing function verification; and application of the adaptive gene. The invention develops an innovative and systematic method combining phenotypic analysis and transcriptomics technologies, and the method is used for accurately excavating adaptive genes of rice in a low-phosphorus stress environment. Through the method, rice individuals with remarkable phenotypic variation under the low-phosphorus condition can be efficiently screened out, the positions of related genes are determined by means of an advanced gene positioning technology, gene expression changes are comprehensively analyzed by means of transcriptome sequencing, and finally the effect of key genes is determined through functional verification. Abundant and key gene resources are provided for cultivating a new variety of low-phosphorus-resistant rice, and a solid theoretical foundation is laid.

This invention relates to the field of functional molecular marker technology, specifically to a DNA molecular marker related to haplotypes of rice cold-resistance genes and its application. The rice cold-resistance gene COLD1 haplotype is composed of SNP145938700 and SNP145938916. When the bases corresponding to these two SNPs are AG, it is a favorable haplotype (Hap2) of the COLD1 gene, indicating strong cold resistance during the budding stage. The molecular marker provided by this invention can be used for haplotype analysis of the COLD1 gene in different rice materials to determine their cold resistance during the budding stage. This allows for the screening of breeding materials with cold resistance during the budding stage through molecular markers, which is more time-saving, labor-saving, efficient, and cost-effective than traditional phenotypic screening. Furthermore, it can be applied to molecular breeding, improving breeding efficiency.

The invention provides a method for breeding a same-grain corn inbred line with a dynamic sweet-glutinous transformation characteristic, and belongs to the technical field of crop genetic breeding. The method comprises the following steps: hybridizing a mutant sh1 homozygous inbred line as a male parent and a waxy wx homozygous inbred line as a female parent to obtain F1, screening and locking a waxy base and a shrinkage form through a stepped phenotype of F2 generation, and carrying out time-phased dynamic detection on F3 generation to screen out a strain of which the soluble sugar content is continuously and obviously higher than that of common waxy corn within 18-35 days after pollination and the waxy property is stably shown. And through backcross reinforcement and high-strength stability screening based on a double-threshold elimination method, finally obtaining the corn inbred line with stable agronomic characters, sweet-waxy dynamic transformation characteristics and a fresh and sweet base taste in a mature period. Molecular marker assistance is not needed, the breeding cost is effectively reduced, and the selection efficiency is improved.

The invention discloses a breeding method and application of a space-carrying rice mutant strain Diantun 502 aviation M6-8, and relates to the technical field of rice breeding, and the technical key points are as follows: the method comprises the following steps: by taking conventional fragrant soft rice 'Diantun 502' breeder's seeds with the amylose content of 8.2% as materials, carrying out space-carrying space mutagenesis to obtain SP0-generation seeds; then through ground multi-generation system breeding, phenotypic screening and STR molecular marker identification are combined, and excellent mutant strains with soft rice characters, fragrance, precocity, strong tillering and rice blast resistance are screened out; and finally, selecting the fragrant and soft type rice mutant strain 'Diantun 502 aviation M6-8' with excellent comprehensive characters through a multi-point product comparison test. The mutant strain keeps the taste quality of fragrant and soft rice, meanwhile, the yield is increased by 8.1% or above, the rice blast resistance is remarkably enhanced, the growth period is shortened, synergistic improvement of fragrance, softness, morning, abundance and resistance is achieved, and the mutant strain can serve as a new variety to be directly popularized or serve as a high-quality parent to be used for cross breeding.

The invention discloses a molecular marker related to efficient utilization of slash phosphorus and application of the molecular marker, and belongs to the technical field of molecular biology. The molecular marker is two missense mutation SNP (Single Nucleotide Polymorphism) sites: SNP-1 (620th site, C-G) and SNP-2 (1897th site, AG-GC) located in a slash pine PeSTOP1 gene coding region. Specific genotypes (a GG type of SNP-1 and a GC / GC type of SNP-2) are obviously related to high phosphorus utilization efficiency and a high-low-phosphorus-resistance comprehensive evaluation value. The invention further provides a primer pair and a kit for detecting the marker and an application method of the primer pair and the kit in auxiliary screening of the slash pine with efficient phosphorus utilization. Through the molecular marker, rapid and accurate genotype identification can be realized in the seedling stage, the defects of long period and low efficiency of traditional phenotype screening are overcome, and a core tool is provided for efficient utilization and genetic improvement of slash pine phosphorus and molecular marker assisted breeding.

The invention discloses a method for screening fat key genes through whole genome knockout, and relates to the technical field of biology. According to the invention, a whole genome knockout system suitable for chicken preadipocyte lines (ICP) is established based on a strategy of improving lentivirus infection efficiency by a cationic co-infection agent, and lipid droplet phenotype screening is carried out in combination with flow cytometry SSC signals, so that key genes influencing fat deposition can be efficiently and systematically screened; and a new technical platform is provided for poultry fat metabolism research and low-fat breeding. Functional verification is performed on the CYP46A1 gene screened based on the system, and results show that after the gene is knocked out, lipid droplet accumulation of chicken preadipocytes is remarkably reduced, expression of lipid metabolism key genes is reduced, and the effect of the CYP46A1 gene in fat deposition regulation is defined.

The invention discloses a method for preparing a plant haploid by using a microspore specific expression NtBBM1 or NtBBM2 gene and application of the method in preparation of angiosperm haploid, and belongs to the field of haploid breeding. Tobacco microspore specific expression transcription factors NtBBM1 or NtBBM2 are directly cultured through a culture medium, microspore embryogenesis can be carried out without stress treatment, plants are cultured, and haploids are obtained through phenotypic screening. According to the method, the dependency of traditional microspore embryogenesis on species genotypes and culture conditions is overcome, a new thought is provided for the biological effect of the BBM gene in the haploid induction process, and the method can improve the crop haploid breeding efficiency.

The invention provides a method for cloning a broad-spectrum resistance gene ETD1 of rice blast through map-based cloning. The method comprises the following steps: phenotypic screening and population construction: extracting DNA from screened individuals; constructing a DNA pool; performing high-throughput sequencing; controlling data quality; performing comparison and variation detection; analyzing variation sites; performing correlation analysis; and predicting candidate genes. The broad-spectrum resistance gene ETD1 cloned by the method has a nucleotide sequence as shown in SEQ ID NO: 1, and / or a cDNA sequence of the gene is as shown in SEQ ID NO: 2, and / or a coding protein of the ETD1 gene has an amino acid sequence as shown in SEQ ID NO: 3, and the gene belongs to a super-efficient allele of OsCNGC13, obtains stronger calcium ion transport capacity, and can be used for preparing a calcium ion transport agent. The function of remarkably accelerating cell calcium ion influx is realized. Therefore, the rice resistance can be improved by normal expression or overexpression of the gene in a gene knockout type plant, and the rice blast resistance can be positively regulated and controlled.