32 results about "Phenotypic screening" patented technology

The invention provides an assay system for identifying a hydrogen-gas-producing organism, including a sensor film having a first layer comprising a transition metal oxide or oxysalt and a second layer comprising hydrogen-dissociative catalyst metal, the first and second layers having an inner and an outer surface wherein the inner surface of the second layer is deposited on the outer surface of the first layer, and a substrate disposed proximally to the outer surface of the second layer, the organism being isolated on the substrate.

The invention relates to an application of an Arabidopis thaliana transcription factor at3g46080 gene. By constructing a CDS sequence of the Arabidopsis thaliana at3g46080 gene into the upstream of aFLAG protein label by a Gateway technology, Arabidopsis thaliana is conversed, which affects the disease phenotype of Arabidopsis thaliana, screening and identification are carried out, and finally the transgenic plants can be obtained. The analysis of the screened plants carried out so that the plant has enhanced resistance to Pseudomonas lycoposum by Arabidopis thaliana, the gene has the same function for homologous genes in crops, and thus has important significance in production.

The invention provides a high-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds. The high-yield crossbreeding method comprises the following steps: A, carrying out phenotypic screening on a GuiKe-2S large group, namely, planting a GuiKe-2S group of a single plant; B, detecting a specificity marker of a GuiKe-2S sterile locus; C, detecting a molecular marker of a GuiKe-2S fingerprint spectrum; D, carrying out low-temperature screening on a GuiKe-2S first-time manual climatic box; E, carrying out low-temperature screening on a GuiKe-2S second-time manual climatic box; and F, breeding a rice root in winter of Hainan, transplanting the GuiKe-2S the root of which is cut again to Sanya of Hainan for breeding in winter, and harvesting seeds when ripening to obtain the two-line super rice parent GuiKe-2S stock seeds. According to the high-yield crossbreeding method, the stock seeds are further bred to obtain high-purity parent seeds applied to seed production, which has a very important significance in developing two-line hybrid rice. Through tests, the purity of the obtained breeder seeds reaches above 99.99 percent, and the purity of the stock seeds obtained through expanding propagation reaches above 99.9 percent.

The invention discloses a method for accurately and rapidly identifying a drug target of an active compound obtained by phenotypic screening. The seamless joint of compound phenotypic screening and target identification is established by a chemical proteomics-driven caenorhabditis elegans feeding RNAi technology; the active compound is an active compound with a corresponding phenotype in the elegans; the phenotype includes but is not limited to lipid dysbolism, cell apoptosis, autophagy, senility, neurodegenerative disease and anti-infection. The problem that at present, false positive and false negative are easily produced when the proteomics method is purely used for analyzing the interacting protein of drug can be solved, and the aim of eliminating the false and retaining the true can be realized; furthermore, the method is simple and convenient in operation and rapid and accurate in screening, and provides a feasible scheme for the analysis of the large-flux interacting protein of the drug; a more perfect chemical proteomics technology system is formed, and the possibility of breaking through the choke point of phenotypic screening protein target identification is provided.

The invention discloses an SSR molecular marker for screening the content and phenotype of apple fruit sugar and application thereof. The SSR molecular marker SugarA3 is successfully developed by determining candidate molecular markers through content and phenotype data of genetic typing related sugar. PCR amplification is conducted through the SugarA3, a simple sequence repetitive unit (AG)n is determined in combination with sequencing, and accordingly the content of the fruit sugar is estimated. Three types of genes can be generated through the SugarA3 and include (AG)9/9, (AG)6/9 and (AG)6/6 or 11, wherein one or two (AG)9 sequence motifs indicate that the corresponding content of fruit sugar and glucose in apple fruits is remarkably lower than that of the apple species without (AG)9 sequence motifs. Thus, the sweetness screening of applies in the seedling period can be easily realized by applying the molecular marker SugarA3, the breeding age limit of the excellent species meetingthe market demands is shortened, and the apple quality and breeding efficiency are improved.

The invention provides a specific DNA (deoxyribonucleic acid) molecular marker and a DNA bar code of trichiurus lepturus and a method for quickly identifying and purifying germplasm of trichiurus lepturus. Aiming at an existing problem of germplasm mixing of the triplophysa zuellii, stable triplophysa zuellii germplasm resources are finally screened out through phenotypic screening, DNA bar code screening and triplophysa zuellii specific DNA molecular marker screening and identification. The method for rapidly purifying the germplasm of the trichiurus punctatus is simple to operate, high in accuracy, short in period, good in stability and high in germplasm purity, and provides technical support for germplasm protection, reasonable utilization and germplasm biodiversity.

The invention belongs to the field of utilization and research of mild plant resources, and particularly relates to a method for breeding a glyphosate-resistant/tolerant novel germplasm by utilizing wild soybeans. The method for breeding the glyphosate-resistant/tolerant novel germplasm utilizes different concentrations of glyphosate to treat the wild soybeans at the seedling stage to screen out wild soybeans with resistance/tolerance to the glyphosate, and then utilizes the screened wild soybeans with resistance/tolerance to carry out interspecific hybridization with cultivated soybeans, so that an interspecific hybridization generation is obtained, glyphosate resistance/tolerance screening is carried out on the selfing line of the interspecific hybridization generation, and in combination with phenotypic screening, the method breeds out the novel germplasm with resistance/tolerance to the glyphosate. The invention determines a wild soybean resource resistance standard evaluation system, and creates the method for screening the wild soybeans with resistance/tolerance, moreover, by utilizing the wild soybeans with resistance/tolerance to carry out interspecific hybridization, the invention creates the method for breeding the glyphosate-resistant/tolerant novel germplasm, and the method is simple, practical and highly efficient, and is also applicable to the research of the other characters of the wild soybeans.

The invention relates to a creation method of giant flower pop corn. The method comprises the following steps of (1) selecting seeds and accelerating germination, namely selecting pop corn selfing line seeds with the large grain size, the large flower size and the high expansion coefficient, and accelerating germination for the first time; (2) performing doubling treatment, namely enabling a chromosome doubling treatment agent to be any one of amiprophos-methyl, trifluralin and colchicine of herbicide; (3) performing breeding; (4) performing phenotype screening and fructification identification; and (5) performing combination and preparation, namely, adopting doubled selfing line seeds as parents, and performing hybridization and matching to obtain a high-yield and high-expansion-coefficient pop corn hybrid. According to the method, a conventional corn chromosome doubling technology is used for reference, large-grain large-flower pop corn is created through a ploidy breeding approach of autotetraploid, large-flower and high-expansion-coefficient germplasm is used as a test material, namely, on the basis of large flowers, the size of the corn flowers is greatly increased again, a giant flower pop corn new variety is bred, and the yield can be increased.

Described herein are methods and compositions for high efficiency transfection of siRNA into a cell population. Such methods and compositions utilize a low voltage pre-conditioning pulse to modulate the efficiency of siRNA transfection. In some embodiments, the methods and compositions permit spatial and temporal control of siRNA transfection efficiency within a population of cells. The disclosed methods and compositions, in some embodiments, are amenable to high throughput applications such as siRNA library-based phenotypic screening.

The invention discloses a method for obtaining mutant strains with the prolonged growth period through compound mutation of dwarf Phaseolus vulgaris L. through a physical method, and belongs to the field of nuclear agricultural science. The method aims to solve the problem that the fresh pod yield of the dwarf Phaseolus vulgaris L. is relatively low. The method for obtaining the mutant strains with the prolonged growth period comprises the following steps that 1, dry seeds of the dwarf edible pod Phaseolus vulgaris L. are placed into ion implantation equipment for nitrogen ion implantation, and the seeds subjected to nitrogen ion implantation are cultivated in a field so as to obtain M1-generation seeds; 2, <60>Co-gamma ray irradiation treatment is carried out on the M1-generation seeds, and the seeds subjected to irradiation treatment are cultivated in the field so as to obtain M2-generation seeds; and 3, the M2-generation seeds are continuously cultivated, and phenotypic screening iscarried out so as to obtain the mutant strains with the prolonged growth period, and therefore the Phaseolus vulgaris L. mutant strains with the prolonged growth period are obtained. According to thecompound mutation method, Phaseolus vulgaris L. mutant germplasm with prolonged growth period can be obtained; and N<+> ion implantation and <60>Co-gamma ray irradiation compound treatment are carried out on the dwarf Phaseolus vulgaris L., 209 mutant strains with the prolonged growth period are obtained, and the growth period can be prolonged by 10-50 days.

The present invention is directed to a method of identifying plants with enhanced agronomic traits within a larger population of plants grown from a randomly planted set of seeds.

The invention relates to a cultivation method of polyploidy of triptonium nigrum, and belongs to the technical field of polyploidy induction. The method disclosed by the invention comprises the following steps of soaking the leucotinum leucotinoides protonemata by using a colchicine solution to obtain mutagenized leucotinum leucotinoides protonemata; carrying out continuous subculture on the mutagenized hypnum macranthum protonemata for three times to obtain subcultured protonemata; carrying out ploidy identification on gametophytes differentiated from the subcultured protonemata to obtain genomic doubled mutants; carrying out subculture on the genome doubled mutant for five times, and screening out a genome doubled and stably inherited mutant; and observing the phenotype of the mutant with doubled genome and stable inheritance, and screening the triptonia melanoides mutant with a brand new phenotype as a new triptonia melanoides strain. The cultivation method is simple to operate, the occurrence rate of chimeras can be reduced, and the obtained polyploidy is stable in heredity.

The invention relates to an application of PsPIWI-RE (Pseudomonas stutzeri-derived PsPIWI-RE protein) in mediating homologous recombination. The research finds that the PsPIWI-RE protein derived from the pseudomonas stutzeri affects DNA (deoxyribonucleic acid) replication and a DNA damage repair way; the invention discloses construction and application of a PsPIWI-RE protein mediated escherichia coli gene knockout technology derived from Pseudomonas stutzeri. The method comprises the following steps: firstly, constructing PsPIWI-RE protein expression plasmids from pseudomonas stutzeri and recombinant plasmids with left and right homologous arm sequences of gene sequences to be knocked out; and then transforming escherichia coli by using two plasmids, inducing double-exchange recombination, and obtaining a strain of which a target gene is knocked out through phenotypic screening or PCR (Polymerase Chain Reaction) identification. According to the operating system, the gene editing system based on the PsPIWI-RE protein and the homologous sequence is constructed for the first time. And a new excellent tool is provided for gene editing of microorganisms such as escherichia coli.

The present invention includes a method, kits, and assays for identifying a human subject as having an increased risk of developing an autoimmune disease, or a human subject with multiple sclerosis caused by elevated soluble Interleukin 7 receptor (sIL7R), by obtaining a biological sample and detecting or measuring in the biological sample an amount of a soluble Interleukin-7 receptor (sIL7R) and an amount of an RNA Helicase DDX39B, whereby a lower expression of DDX39B and a higher secretion of sIL7R identifies the subject from which the biological sample was obtained as having an increased risk of developing an autoimmune disease, when compared to a human subject not having an autoimmune disease. The present invention also includes a method of modifying a treating of subjects based on the lower expression of RNA Helicase DDX39B alone or in combination with an increase in sIL7R.