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Method for obtaining mutant strains with prolonged growth period through compound mutation of dwarf Phaseolus vulgaris L. through physical method

A technology of composite mutagenesis and physical methods, applied in the field of nuclear agronomy, can solve the problems of low yield of dwarf lettuce bean fresh pods, and achieve the effects of improving actual benefits, increasing yield and high yield

Active Publication Date: 2021-03-12
TECHN PHYSICS INST HEILONGJIANG ACADOF SCI
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Problems solved by technology

[0005] The purpose of the present invention is in order to solve the problem that the fresh pod yield of dwarf bean is lower, and provide a kind of physical method compound mutagenesis dwarf bean to obtain growth period prolongation method for mutants

Method used

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  • Method for obtaining mutant strains with prolonged growth period through compound mutation of dwarf Phaseolus vulgaris L. through physical method
  • Method for obtaining mutant strains with prolonged growth period through compound mutation of dwarf Phaseolus vulgaris L. through physical method
  • Method for obtaining mutant strains with prolonged growth period through compound mutation of dwarf Phaseolus vulgaris L. through physical method

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specific Embodiment approach 1

[0013] Specific implementation mode one: the physical method of this embodiment combined mutagenesis of dwarf bean to obtain the method for prolonging the growth period mutants is realized according to the following steps:

[0014] 1. Place the dry seeds of dwarf viburnum in ion implantation equipment for nitrogen ion implantation, and control the nitrogen ion injection rate to 3×10 16 N + / cm 2 ~5×10 16 N + / cm 2 , the seeds after nitrogen ion implantation were cultivated in the field to obtain M1 generation seeds;

[0015] Second, carry out the M1 generation seed 60 Co-γ-ray irradiation treatment, the irradiation dose is controlled at 20Gy-150Gy, and the irradiated seeds are cultivated in the field to obtain M2 generation seeds;

[0016] 3. Continue to cultivate the M2 generation seeds, and obtain mutants with extended growth period through phenotype screening, and obtain mutants of kidney bean with extended growth period.

specific Embodiment approach 2

[0017] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the variety of the dwarf pod-eating bean described in step one is A18-1 (the whole growth period is about 55 days).

[0018] In this embodiment, the dwarf pod-eating bean is provided by the Crop Research Institute of Heilongjiang University.

specific Embodiment approach 3

[0019] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that in Step 1, the energy of implanting nitrogen ions is controlled to be 30 keV.

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Abstract

The invention discloses a method for obtaining mutant strains with the prolonged growth period through compound mutation of dwarf Phaseolus vulgaris L. through a physical method, and belongs to the field of nuclear agricultural science. The method aims to solve the problem that the fresh pod yield of the dwarf Phaseolus vulgaris L. is relatively low. The method for obtaining the mutant strains with the prolonged growth period comprises the following steps that 1, dry seeds of the dwarf edible pod Phaseolus vulgaris L. are placed into ion implantation equipment for nitrogen ion implantation, and the seeds subjected to nitrogen ion implantation are cultivated in a field so as to obtain M1-generation seeds; 2, <60>Co-gamma ray irradiation treatment is carried out on the M1-generation seeds, and the seeds subjected to irradiation treatment are cultivated in the field so as to obtain M2-generation seeds; and 3, the M2-generation seeds are continuously cultivated, and phenotypic screening iscarried out so as to obtain the mutant strains with the prolonged growth period, and therefore the Phaseolus vulgaris L. mutant strains with the prolonged growth period are obtained. According to thecompound mutation method, Phaseolus vulgaris L. mutant germplasm with prolonged growth period can be obtained; and N<+> ion implantation and <60>Co-gamma ray irradiation compound treatment are carried out on the dwarf Phaseolus vulgaris L., 209 mutant strains with the prolonged growth period are obtained, and the growth period can be prolonged by 10-50 days.

Description

technical field [0001] The invention belongs to the field of nuclear agronomy, in particular to a kind of N + ion implantation with 60 Co-γ-ray irradiation compound treatment method of dwarf bean, thereby creating mutants with extended growth period. Background technique [0002] Kidney beans (Phaseolus vulgaris L.), also known as kidney beans, are edible beans with the largest planting area in the world. Rich in nutrition and high in protein content, it is cultivated all over the world. It is both a vegetable and a grain. It is an important agricultural and sideline product for export and foreign exchange. Planting kidney beans plays an important role in the adjustment of agricultural planting structure. Heilongjiang Province is an important production area of ​​high-quality kidney beans. Varieties such as "Yiandianhong" and "Jiangjun Bean" have entered Beijing, Shanghai, Guangzhou, and even Xinjiang, and the market prospects are excellent. [0003] At present, the produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 斯琴图雅赵弘韬孙培琳关荣树张丹丹史岩
Owner TECHN PHYSICS INST HEILONGJIANG ACADOF SCI
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