Method for breeding glyphosate-resistant/tolerant novel germplasm by utilizing wild soybeans

Abstract

The invention belongs to the field of utilization and research of mild plant resources, and particularly relates to a method for breeding a glyphosate-resistant / tolerant novel germplasm by utilizing wild soybeans. The method for breeding the glyphosate-resistant / tolerant novel germplasm utilizes different concentrations of glyphosate to treat the wild soybeans at the seedling stage to screen out wild soybeans with resistance / tolerance to the glyphosate, and then utilizes the screened wild soybeans with resistance / tolerance to carry out interspecific hybridization with cultivated soybeans, so that an interspecific hybridization generation is obtained, glyphosate resistance / tolerance screening is carried out on the selfing line of the interspecific hybridization generation, and in combination with phenotypic screening, the method breeds out the novel germplasm with resistance / tolerance to the glyphosate. The invention determines a wild soybean resource resistance standard evaluation system, and creates the method for screening the wild soybeans with resistance / tolerance, moreover, by utilizing the wild soybeans with resistance / tolerance to carry out interspecific hybridization, the invention creates the method for breeding the glyphosate-resistant / tolerant novel germplasm, and the method is simple, practical and highly efficient, and is also applicable to the research of the other characters of the wild soybeans.

Description

technical field [0001] The invention belongs to the field of wild plant resource utilization research, in particular to a method for breeding glyphosate-resistant new germplasm by using wild soybeans. Background technique [0002] Glyphosate is a very important organophosphorus herbicide. It is a foliar, broad-spectrum, non-selective herbicide that has strong control over annual and perennial weeds. [0003] The glyphosate-resistant gene is transferred into the transgenic soybean, so that the sensitivity of the transgenic soybean to glyphosate is relatively low, and the shikimic acid pathway in the transgenic soybean plant can proceed normally, thus showing resistance to glyphosate. Since GM soybeans were approved for commercial production in the United States in 1994, the global acreage of GM soybeans has increased rapidly. However, due to the large-scale planting of glyphosate-resistant transgenic soybeans and the long-term and single use of glyphosate, the frequency of g...

AI Technical Summary

Problems solved by technology

However, due to the large-scale planting of glyphosate-resistant transgenic soybeans and the long-term and single use of glyphosate, the frequency of glyphosate-resistant weeds in the field has increased significantly; or other herbicides, the transgenic soybean plants will not be able to quickly decompose glyphosate, thus appearing symptoms of phytotoxicity; at the same time, during the selection of resistant varieties, some varieties will be less resistant to glyphosate than other varieties, As a result, genetically modified soybeans have phytotoxicity problems after spraying glyphosate

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