High-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds

Abstract

The invention provides a high-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds. The high-yield crossbreeding method comprises the following steps: A, carrying out phenotypic screening on a GuiKe-2S large group, namely, planting a GuiKe-2S group of a single plant; B, detecting a specificity marker of a GuiKe-2S sterile locus; C, detecting a molecular marker of a GuiKe-2S fingerprint spectrum; D, carrying out low-temperature screening on a GuiKe-2S first-time manual climatic box; E, carrying out low-temperature screening on a GuiKe-2S second-time manual climatic box; and F, breeding a rice root in winter of Hainan, transplanting the GuiKe-2S the root of which is cut again to Sanya of Hainan for breeding in winter, and harvesting seeds when ripening to obtain the two-line super rice parent GuiKe-2S stock seeds. According to the high-yield crossbreeding method, the stock seeds are further bred to obtain high-purity parent seeds applied to seed production, which has a very important significance in developing two-line hybrid rice. Through tests, the purity of the obtained breeder seeds reaches above 99.99 percent, and the purity of the stock seeds obtained through expanding propagation reaches above 99.9 percent.

Description

[0001] technical field [0002] The invention belongs to the field of agricultural technology, and relates to a high-quality and high-yield propagation method, in particular to a high-quality and high-yield propagation method for the original species of the two-line super rice parent Guiceae-2S. [0003] Background technique [0004] The two-line male sterile line of hybrid rice based on the light-temperature-sensitive male sterile line has a good application prospect due to its advantages of free combination, dual-purpose of one line, and simplified breeding procedure. At present, the bred photothermosensitive male sterile lines Pei'ai 64S, Zhu 1S, Y58S and Guangzhan 63S have been widely used in production. However, the two-line sterile lines of hybrid rice are susceptible to the influence of light and temperature, which causes the seed of the sterile lines to be mixed, which brings huge risks to the seed production of two-line hybrid rice. In order to reduce the potentia...

AI Technical Summary

Problems solved by technology

However, the two-line sterile lines of hybrid rice are susceptible to the influence of light and temperature, causing the seeds of the sterile lines to be mixed, which brings huge risks to the seed production of two-line hybrid rice.