A method for accurate and rapid identification of drug targets of active compounds obtained by phenotypic screening

A compound and active technology, applied in the field of medicine, can solve problems such as unknown targets, and achieve the effect of acceleration speed and precision
CN104237530BActive Publication Date: 2016-01-06RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Publication Date
2016-01-06

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Abstract

The invention discloses a method for accurately and rapidly identifying a drug target of an active compound obtained by phenotypic screening. The seamless joint of compound phenotypic screening and target identification is established by a chemical proteomics-driven caenorhabditis elegans feeding RNAi technology; the active compound is an active compound with a corresponding phenotype in the elegans; the phenotype includes but is not limited to lipid dysbolism, cell apoptosis, autophagy, senility, neurodegenerative disease and anti-infection. The problem that at present, false positive and false negative are easily produced when the proteomics method is purely used for analyzing the interacting protein of drug can be solved, and the aim of eliminating the false and retaining the true can be realized; furthermore, the method is simple and convenient in operation and rapid and accurate in screening, and provides a feasible scheme for the analysis of the large-flux interacting protein of the drug; a more perfect chemical proteomics technology system is formed, and the possibility of breaking through the choke point of phenotypic screening protein target identification is provided.
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Description

technical field

[0001] The present invention relates to the field of medicine, and more specifically, relates to a method for accurately and rapidly identifying drug targets of active compounds obtained by phenotypic screening. Background technique

[0002] Drug discovery strategies mainly include "phenotype-based screening (phenotypic screening)" and "target-based screening (target-based screening)". These two types of drug screening have successively dominated drug development for the past hundred years. The former focuses on compound-induced effects or phenotypes in cells, tissues, or whole organisms, while the latter analyzes the effects of compounds on purified target proteins in vitro. Among them, target-based drug screening has dominated in the past 30 years. However, the statistical analysis of new drugs approved by the FDA from 1999 to 2008 shows that although target-based screening has an advantage in the approval of follower tracking drugs, there are only 17 dis...

Claims

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