38 results about "Neural tube" patented technology

A method of differentiating embryonic stem cells into oligodendroglial precursor cells and oligodendroglial cells by culturing a population of cells comprising a majority of cells that are characterized by a neural tube-like rosette morphology and are Pax6+ / Sox1+ into a population of cells that are PDGFRα+.

The present invention is to provide an artificial tube for nerve which remains in a body until a nerve regenerates, does not remain in the body as a foreign body after regeneration of the nerve, induces axons regenerated from severed nerve stumps, promotes infiltration of blood capillaries from the body and promotes regeneration of nerve tissue, and a method for producing the same. The artificial tube for nerve has fine fibrous collagen bodies (30) in the lumen of a tube comprised of a biodegradable and absorbable material, the voids inside the fine fibrous collagen bodies being filled with laminin.

A brachial plexus simulator is disclosed. The brachial plexus simulator comprises a head, a spinal assembly, and a simulated brachial plexus. The head may be weighted to a biofidelic weight and may include a tilt sensor. The spinal assembly comprises a simulated spine mounted for extension. The simulated brachial plexus comprises a network of nerve tubing that is attached relative to the simulated spine at appropriate points so as to mimic the connectivity, shape, and compliance of the brachial plexus. The nerve tubing may be filled with a fluid material whose resistance or other electrical properties change with its length, such as mercury. Maternal and fetal birthing simulators are also disclosed.

The invention belongs to the field of biotechnology and medicine and relates to an application of miR-219, a new marker of human brain glioma. The fact that the expressions of miR-219 in the human brain glioma tissues and cells are obviously reduced is confirmed for the first time, and the facts that miR-219 can directly control Hh-Gli2 and Pdgfra signal paths during early development of the neural tube of embryo and occurrence of the brain glioma and can obviously suppress proliferation and infiltration of human glioma cells are confirmed. Further quantitative analysis of clinical brain glioma specimens at different levels shows that miR-219 can serve as the marker for detecting the brain glioma.

The invention discloses a preparation technology of a novel nerve conduit, and belongs to the technical field of tents of biological tissue engineering. The preparation technology sequentially comprises a preparation technology of GDNF (glial-derived neurotrophic factor)-carried gelatin microspheres, a synthesizing technology of gelatin-methacrylamide hydrogel, a preparation method of polycaprolactone / gelatin electricity texture nerve conduits, and a combining method of the electricity texture nerve conduits and the GDNF-carried gelatin microspheres. The preparation technology has the advantages that a tent structure with good degradability, biological compatibility and biological safety is realized, and the GDNF is controlled and released by the GDNF-carried gelatin microspheres; compared with blank groups and collagen conduit groups, the regeneration of peripheral nerves is effectively promoted, and the structure and function of restoring damaged nerves can reach the effect similar to the effect of autotransplantation groups; the defect nerves can be bridged, and the stability of axon regeneration environment can be maintained; the invasion of peripheral connective tissues is prevented; the saturing of near and far-heart breaking ends of smaller nerve defect is solved, and the restoration of larger nerve defect is solved.

An oral cavity CBCT image mandibular neural tube automatic recognition method based on Mask RCNN comprises the following steps: collecting oral cavity CBCT image data, preprocessing a coronal diagramin the oral cavity CBCT image data, removing an image which does not display a mandibular neural hole in the coronal diagram, performing format compression on the image, and manually aiming at the mandibular neural hole; generating a sighting frame, positioning the rectangular frame, obtaining a binary mask mask of the target instance, and establishing a neural network model; training a model; andrecognizing and displaying the mandibular nerve hole through the trained model. According to the method, the Mask RCNN is used for automatically identifying the mandibular neural tube in the CBCT image. Machine recognition is used for replacing manual recognition of coronal diagram tooth nerve holes, so that the labor cost is saved, the stability of the generated mandibular nerve tube track is improved, and the method has good recognition speed and precision.

The present invention relates to a pregestational gene alarm diagnosis kit and its detection method, including DNA extraction liquor for extracting DNA template of testee, PCR mixed liquor for amplifying specific fragment on the genomic DNA of testee, DNA polymerase, PCR product enzyme excision mixed liquor, restriction endonuclease, positive control template of homozygous mutation and coupling pipe for making PCR reaction. The described alarm gene includes N5, N10-methylene tetrahydrofolate reductase and methionine synthetase reductase gene, their mulant sites are respectively 667-cytosine-thymine and 66 adenine-guanine, and their primer sequences respectively are 5'-TGA AGG AGA AGG TGT CTG CGG GA-3', 5'-AGG ACG GTG CGG TGA GAG TG-3', 5'-GCA AAG GCC ATC GCA GAA GAC AT-3' and 5'-GTG AAG ATC TGC AGA AAA TCC ATG TA-3'. It can be used for pregestational diagnosis of that filial generation produces neural tube deform or not.

The invention discloses a method for constructing brain-like tissue by using umbilical cord mesenchymal stem cells, which comprises the following steps: 1) carrying out stem cell culture on a human umbilical cord mesenchymal stem cell line to obtain subculture cells; 2) carrying out nerve induction differentiation on the subculture cells to obtain differentiated neuronal precursor cells which areembryoid-like nerve spheres; 3) carrying out induction culture on the embryoid-like nerve spheres to obtain a neural rosettte neural tube-like structure neural spheres; and (4) carrying out differentiation culture on the neural rosettte neural tube-like structure neural spheres to obtain a human brain-like organ which contains a plurality of types of neuron mixtures. The human mesenchymal stem cells are differentiated into various neurons for the first time, the brain-like tissue is constructed, the brain-like tissue can be used as a drug screening platform, and the application prospect is wide.

The invention relates to the field of bioengineering and particularly relates to a neural restoration promoting tube as well as a preparation method and application thereof. In order to overcome the problem of unsatisfactory neural functional recovery of current direct nerve suture operation, the invention provides a neural restoration promoting tube with a shape memory function. The neural restoration tube is prepared and formed by injecting a gelatin solution into a neural restoration tube mould; and the neural tube mould can be a neural restoration tube mould prepared by 3D printing. The neural restoration promoting tube has a shape memory function; and after being implanted into a body, the tube can recover to the original shape under the effect of water and automatically extends along the axial direction of nerves so as to better wrap and cover the periphery of the injured nerves and provide a stable microenvironment for the nerve growth. Moreover, the wounds of the nerves separated by operation can be greatly reduced, the wound recovery is facilitated, the functional recovery of the nerves is promoted, and the clinical application prospect is perfect.

The invention discloses a method of non-exogenous induction of a pluripotent stem cell to be a GABA neuron. The method includes the following steps of 1 cultivating a pluripotent stem cell in a Matrigel or a Vitronectin matrigel system to the density of 60 % -80 %; 2 digesting the pluripotent stem cell obtained in step 1 with Dispase or EDTA (Ethylene Diamine Tetraacetic Acid), differentiating the suspension culture through neural induction on that day, and recording the day as the 0 day; 3 adding neural induction differentiation solution for further cultivation when a spherical cell formed by the pluripotent stem cell can be observed; 4 performing adherence on the seventh day; 5 adding SHH or a small molecule compound Purmophine on the tenth day for continuous induction; 6 blowing off a formed neural tube structure on the sixteenth day and continuing to cultivate with the neural induction solution; 7 obtaining the GABA neuron on the twenty-second day. The method of non-exogenous induction of the pluripotent stem cell to be the GABA neuron can significantly improve cultivation solution complication and low differentiation rate and eliminate the interference of exogenous factors.

A neural tube capable of complexly playing roles of a support for regenerating a nerve and a nerve electrode has a support connected to a terminal of an injured nerve, and a sieve electrode having an electrode hole formed in a body thereof and a circular electrode formed around the electrode hole, wherein the body of the sieve electrode is buried in the support, wherein a cavity-type channel is formed at the support to extend to the inside of the support, wherein the electrode hole is aligned with the channel, and wherein a nerve cell growing along the channel at the terminal of the injured nerve is capable of contacting the circular electrode.

A self-renewing restricted stem cell population has been identified in developing (embryonic day 13.5) spinal cords that can differentiate into multiple neuronal phenotypes, but cannot differentiate into glial phenotypes. This neuronal-restricted precursor (NRP) expresses highly polysialated or embryonic neural cell adhesion molecule (E-NCAM) and is morphologically distinct from neuroepithelial stem cells (NEP cells) and spinal glial progenitors derived from embryonic day 10.5 spinal cord. NRP cells self renew over multiple passages in the presence of fibroblast growth factor (FGF) and neurotrophin 3 (NT-3) and express a characteristic subset of neuronal epitopes. When cultured in the presence of RA and the absence of FGF, NRP cells differentiate into GABAergic, glutaminergic, and cholinergic immunoreactive neurons. NRP cells can also be generated from multipotent NEP cells cultured from embryonic day 10.5 neural tubes. Clonal analysis shows that E-NCAM immunoreactive NRP cells arise from an NEP progenitor cell that generates other restricted CNS precursors. The NEP-derived E-NCAM immunoreactive cells undergo self renewal in defined medium and differentiate into multiple neuronal phenotypes in mass and clonal culture. Thus, a direct lineal relationship exists between multipotential NEP cells and more restricted neuronal precursor cells present in vivo at embryonic day 13.5 in the spinal cord. Methods for treating neurological diseases are also disclosed.

The invention provides an animal-derived nerve scaffold which comprises a scaffold membrane material and degradable metal wires wrapped in the scaffold membrane material, wherein main components of the scaffold membrane material are animal-derived blood vessels, lymphatic vessels, neural tubes or intestinal tracts. The animal-derived nerve scaffold is derived from animals, so that the problem of short supply of autologous nerves is solved, the problem of donor site function damage caused by new nerve injury inevitably caused by autologous neural transplantation is also solved, and the nerve scaffold has far-reaching significance for nerve repair.

Provided are genetically engineered cells comprising a neural stem cell and retroviral expression system in the neural stem cell, which is capable of expressing homeodomain transcription factor Nkx6.1 protein but does not express homeodomain transcription factor Irx3 protein or homeodomain transcription factor Nkx2.2 protein; which is capable of expressing homeodomain transcription factor Nkx6.1 protein and homeodomain transcription factor Irx3 protein; and which is capable of expressing homeodomain transcription factor Nkx2.2 protein or homeodomain transcription factor Nkx2.9 protein. Also provided are methods of generating such genetically engineered motor neurons, V2 neurons, and V3 neurons. Also provided are methods of treating subjects having a motor neuron injury or a motor neuron disease comprising implanting in injured / diseased neural tissue of the subject any of the provided genetically engineered cells, administering to such neural tissue retroviral expression systems which are capable of expressing the appropriate homeodomain protein(s), or transfecting neural stem cells with a retroviral vector, which is capable of expressing the required homeodomain transcription factor protein(s). Provided is a method of determining whether a chemical compound affects the generation of a motor neuron from a neural stem cell.

The invention discloses a temporal bone model for surgery training and a forming method thereof, which belong to the technical field of ear surgery training aids. The temporal bone model for surgery training comprises a cortical bone, a cancellous bone, a semicircular canal, an ear bone and a face neural tube, wherein the cancellous bone is of a porous structure; the semicircular canal and the face neural tube are of cavity structures; the ear bone is of a solid structure; the semicircular canal, the ear bone and the face neural tube are integrally formed by adopting aluminum oxide ceramics through 3D (Three-dimensional) printing; the cancellous bone is prepared from three raw materials: mixed powder, water and graphite powder; the mixed powder is prepared from mixing 85 percent by weightof clay and 15 percent by weight of feldspar powder; the cortical bone is prepared from two raw materials: mixed powder and water. The invention provides the temporal bone model for surgery training and the forming method thereof. The temporal bone model for surgery training is higher in biofidelity, lower in manufacturing cost, and capable of greatly improving a surgery training effect, shortening a doctor training period, reducing doctor training cost, and benefitting patients.

The invention discloses a multifunctional oral implant practice model, which comprises a maxillary model and a mandible model. The maxillary model comprises maxillary soft tissue, a maxillary bone, maxillary teeth and a maxillary connection member. The maxillary connection member is fixedly arranged in the middle of the top surface of the maxillary bone; the maxillary soft tissue wraps the maxillary bone part; and the maxillary teeth are fixedly inserted into corresponding maxillary alveolar sockets. The mandible model comprises mandible soft tissue, a mandible bone, mandible teeth, a mandible tongue base, a mandible neural tube and a mandible connection member. The mandible connection member is fixedly arranged in the middle of the bottom surface of the mandible tongue base; the mandible bone circularly wraps the front side and left and right sides of the mandible tongue base, and wraps the whole mandible neural tube; the mandible soft tissue wraps the mandible tongue base and the part, except the bottom surface, of the mandible bone; and the mandible teeth are fixedly inserted into corresponding mandible alveolar sockets. Training contents are arranged in one set of maxillary and mandible oral cavity model as many as possible, thereby achieving the effects of simulation of clinical simulation, operation convenience of a short-term training class and people availability of production cost.

The invention belongs to the technical field of biological medicine, and particularly relates to a molecular marker for antenatal noninvasive diagnosis of a fetal with neural tube malformation, congenital heart disease or cleft lip and palate and application of the molecular marker. The molecular marker is composed of one or more selected from CORO1A, DNM2 and ACTR2 proteins for antenatal noninvasive diagnosis of fetuses with neural tube malformation, congenital heart disease and cleft lip and palate. The molecular marker for antenatal noninvasive diagnosis is applied to preparation of products for antenatal screening, early warning, clinical diagnosis and biochemical inspection of fetuses with neural tube malformation, congenital heart disease and cleft lip and palate. According to the invention, the close correlation between the abnormal expression of the proteins (including CORO1A, DNM2 and ACTR2) in the blood of the pregnant woman and the occurrence of the fetal with the neural tube deformity, the congenital heart disease and the cleft lip and palate is found and confirmed for the first time, the quantity of verified samples is large, the result is accurate, and a new way is provided for the prenatal screening, early warning and diagnosis of the fetal with the neural tube deformity, the congenital heart disease and the cleft lip and palate.

The present invention discloses a composition for accelerating nerve repair. The composition comprises platelet-rich fibrin, a growth factor and a cytokine. The composition may further comprise a tissue with a stem cell or a neural tube to be a scaffold for promoting nerve generation. The platelet-rich fibrin is obtained from the blood of the mammal to be operated on with a nerve repair.

The invention specifically discloses a neural crest stem cell preparation and application thereof. The neural crest stem cell preparation provided by the invention is obtained by culturing a neural tube in vitro, wherein the neural tube is derived from a 10-day pregnant mouse embryo. The neural crest stem cell preparation disclosed by the invention and obtained by culturing the neural tube derivedfrom a 10-day pregnant mouse embryo in vitro identifies neural crest stem cells by immunofluorescence; P0 generation cells and P3 generation cells respectively express characteristics of neural crestand do not express the characteristics of neural stem, which indicates that the cultured cells successfully maintain the characteristics of mouse neural crest stem cells in vitro; and in-vitro culture experiments show that the neural crest stem cells can grow stably in a provided culture medium and normal proliferation of cells is maintained.

The invention relates to a quantum dot fluorescence detection method for folic acid in red blood cells. Folic acid is an essential vitamin in the growth and development process of a human body, and has the effects of preventing neonatal neural tube deformity, reducing anemia and preeclampsia of pregnant women and the like. Therefore, the detection method which is simple and convenient to operate, sensitive and reliable is provided and has important significance for monitoring the folic acid supplement effect of people such as pregnant women. The invention provides the quantum dot fluorescence detection method for folic acid in red blood cells, in order to improve the detection accuracy, pretreatment and dissociation steps for detection are optimized, and full rupture and dissociation of folic acid in red blood cells are realized. The invention further provides a solid-phase chromatography test strip based on quantum dot detection, folic acid detection based on the test strip has good precision and sensitivity, and the test strip can adapt to normal-temperature transportation and has an ideal market prospect.

The embodiment of the invention discloses an automatic marking method and device for a mandibular neural tube and electronic equipment. The method and device are used for marking mandibular neural tube data with high precision. The method comprises the following steps: acquiring first three-dimensional panorama data of a periodontal curved body; expanding the first three-dimensional panorama data into cuboid second three-dimensional panorama data; marking the second three-dimensional panorama data by using a trained 3D neural network model to obtain a mandibular neural tube; wherein the 3D neural network model is obtained by training based on three-dimensional panorama data of a cuboid marked with a mandibular neural tube; and mapping the marked mandibular neural tube to the first three-dimensional panorama data.

The invention provides a training method for simulating tooth implantation. The training method comprises the following steps: S1, acquiring original implant data representing different tooth positions and different bone conditions; S2, acquiring CBCT (Cone Beam Computerized Tomography) data corresponding the original implant data, performing modeling according to the CBCT data, and generating STL (Standard Template Library) data of alveolar bones and teeth; S3, importing neural tube or maxillary sinus or nasal base structure data into the STL data of alveolar bones and teeth; S4, printing a physical model of alveolar bones and teeth according to the STL data imported with the neural tube data in step S3; and S5, performing simulated tooth implantation training using the printed physical model of alveolar bones and teeth. The training method for simulating tooth implantation based on real patient cases is morphologically closer to real patients relative to a high molecular simulation model and animal jaws, so that trainees can be trained on models of real cases.

The invention belongs to the technical field of biological medicines, and particularly relates to a molecular marker for prenatal noninvasive diagnosis of fetuses with cleft lip and palate, neural tube malformation and congenital heart disease and application of the molecular marker. The molecular marker for prenatal noninvasive diagnosis of the fetuses with cleft lip and palate, neural tube malformation and congenital heart disease comprises three piRNAs including hsa-piR-009228, hsa-piR-016659 and hsa-piR-020496. The invention further discloses application of the molecular marker for prenatal noninvasive diagnosis in preparation of products for prenatal screening, early warning, clinical diagnosis and biochemical examination of the fetuses with cleft lip and palate, neural tube malformation and congenital heart disease. The molecular marker and the application have the advantages that gene expression abnormality of piRNAs in blood of pregnant women is discovered and confirmed for thefirst time to be closely related to occurrence of the fetuses with cleft lip and palate, neural tube malformation and congenital heart disease, verified sample sizes are large, results are accurate,and a novel way is provided for prenatal screening, early warning and diagnosis of the fetuses with cleft lip and palate, neural tube malformation and congenital heart disease.

The invention relates to the field of medical image processing, in particular to a mandibular neural tube center line extraction and radius calculation method. The method comprises the following steps: performing mandibular neural tube automatic segmentation on an oral cavity CBCT image by using a segmentation algorithm and a connected domain algorithm; carrying out image refinement on a segmentation result by utilizing a keel extraction algorithm; constructing a graph structure for the keel points of the mandibular neural tube, and generating graph nodes and side information; pruning a keel of the mandibular nerve tube, and extracting a longest path in the keel to obtain a center line point cloud of the mandibular nerve tube; performing curve fitting and down-sampling on the center line point cloud to obtain a mandibular neural tube center line and a control point and a tangent vector of the mandibular neural tube center line; and generating a cross section by using the control point and the tangent vector, and calculating an intersection line of the cross section and the mandibular neural tube three-dimensional model to obtain the radius of the control point. According to the method, the positioning precision of the mandibular nerve tube is improved, and the time cost of manual marking is saved.

The invention discloses a method for screening fetal open neural tube defects (ONTD) by maternal serum alpha-fetoprotein heterogeneity L2 and L3 in the second trimester. The method comprises the following steps: (1) ONTD is confirmed by ultrasound imaging A number of pregnant women with fetuses formed the case group, and multiple pregnant women with normal fetal development were randomly selected to form the control group; (2), the levels of AFP-L2 and AFP-L3 in the serum of two groups of pregnant women were detected by enzyme-linked immunosorbent assay, and the levels of AFP-L2 and AFP-L3 in the second trimester The correlation between AFP-L2 and AFP-L3 levels in women's serum and fetal ONTD was statistically processed, and the screening efficiency of different risk calculation models was analyzed; (3), AFP-L2 and AFP were calculated according to the ROC curve ‑L3 optimal cut-off value and area under the curve for screening ONTD fetuses. The beneficial effect of the present invention is: the screening ONTD fetus of middle pregnancy maternal serum AFP-L2 and AFP-L3 has higher sensitivity and specificity, is a better marker for screening ONTD, using AFP-L2 and AFP-L3 The risk calculation model constructed jointly has better screening efficiency than using AFP, AFP‑L2, and AFP‑L3 alone to screen for MoM values.

The invention discloses a breeding method for black pig breeding, which comprises the following steps: building a pig house for black pigs, then selecting breeding pigs, dividing the breeding pigs into boars and sows, managing the boars and the sows in different houses, feeding the boars and the sows with feed for 3-4 times every day by using feeding equipment, vitamin B is supplemented before mating, so that embryo neural tube malformation and congenital heart diseases can be prevented, sow anemia diseases can also be prevented, boars need to move for 1-2 hours after being fed for half an hour, the growth rate of fat of black pigs can be reduced, pig houses are cleaned and disinfected three times every day according to the movement time of the boars and the sows, and the breeding efficiency is improved. Excrement and urine in the pig house are removed, germs caused by qualitative change of the excrement or the urine are reduced, the living environment of the black pigs is kept clean, the physique of the black pigs is improved, and therefore the breeding quality of the black pigs is improved.

The invention relates to medical instruments and specifically relates to a nerve locator for mandible super-long curve osteotomy. The nerve locator comprises gear sleeves, a main rod, sliding blocks,a locating block and an auxiliary rod, wherein the main rod is marked with scales from top to bottom, the sliding blocks sleeve the main rod, the gear sleeves are connected with the sliding blocks through connecting rods, and the sliding blocks are fixed with the main rod through first screws by virtue of formed screw holes; specifically, the two gear sleeves are respectively arranged on two sidesof the main rod, are more steady during fixation and are respectively connected with the main rod through the two connecting rods and the two sliding blocks, and the connecting rods in an oral cavityof a human body are of a bending shape and bend and extend outwards; and the locating block sleeves the main rod and is fixed with the main rod through a second screw by virtue of a formed screw hole, and the auxiliary rod is arranged on the locating block in a transverse penetrating manner. The nerve locator can be used for marking the position of a mandibular nerve tube during the mandible super-long curve osteotomy, so that effective evading is realized.

An animal-derived nerve scaffold and a manufacturing method therefor. The animal-derived nerve scaffold comprises a scaffold membrane (1) and a degradable metal wire (2) wrapped by the scaffold membrane (1). The primary constituents of the scaffold membrane (1) are animal-derived blood vessels, lymphatic vessels, neural tubes or intestines. The manufacturing method comprises the steps of preparing an animal-derived membrane powder and wrapping the degradable metal wire (2) with the scaffold membrane (1).

The invention relates to the field of bioengineering and particularly relates to a neural restoration promoting tube as well as a preparation method and application thereof. In order to overcome the problem of unsatisfactory neural functional recovery of current direct nerve suture operation, the invention provides a neural restoration promoting tube with a shape memory function. The neural restoration tube is prepared and formed by injecting a gelatin solution into a neural restoration tube mould; and the neural tube mould can be a neural restoration tube mould prepared by 3D printing. The neural restoration promoting tube has a shape memory function; and after being implanted into a body, the tube can recover to the original shape under the effect of water and automatically extends along the axial direction of nerves so as to better wrap and cover the periphery of the injured nerves and provide a stable microenvironment for the nerve growth. Moreover, the wounds of the nerves separated by operation can be greatly reduced, the wound recovery is facilitated, the functional recovery of the nerves is promoted, and the clinical application prospect is perfect.