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44 results about "Oligodendroglial cell" patented technology

Production of oligodendrocytes from placenta-derived stem cells

The present invention provides methods and compositions for the production of glial cells and oligodendrocytes from placenta stem cells. The invention further provides for the use of these glia and oligodendrocytes in the treatment of, and intervention in, for example, trauma, ischemia and degenerative disorders of the central nervous system (CNS), particularly in the treatment of demyelinating diseases such as multiple sclerosis.
Owner:CELULARITY INC

Peripheral nerve field stimulator curved subcutaneous introducer needle with wing attachment specification

An apparatus for use in peripheral nerve field stimulation (PNFS) whereby a plurality of curved introducer needles, of varying curvatures, are provided to permit the physician to best locate the region of oligodendrocytes that contain the A Beta fibers by matching the lumbar lordosis. A wing device is also provided that is attachable to the hub of the curved needle introducer which gives the physician better ability to maneuver the needle during insertion as well as permitting tenting of the skin. The invention benefits a large number of painful disorders arising from pathology in the cervical, thoracic, and lumbar spine. In addition, this invention can also help a large number of other conditions including but not limited to failed back surgery syndrome / post-laminectomy pain, occipital / suboccipital headaches, scar pain, post herpetic neuralgia pain, mononeuritis multiplex, and pain following joint surgery (e.g., knee, hip, shoulder).
Owner:ADVANCED NEUROMODULATION SYST INC

Culture method to obtain and maintain a pure or enriched population of mammalian neural stem cells and/or neural/progenitor cells that are prone to differentiate into oligodendrocyte-lineage cells in vitro

An isolated expandable human neural stem or progenitor cell wherein the cell is a progenitor cells or stem cell, maintains its capability to differentiate into neurons, astrocytes, and oligodendrocytes, maintains its ability to differentiate into oligodendrocyte lineage cells efficiently throughout subsequent passages, and the cell expresses at least cell surface antigens CD133 and CD140α. Also provided is a method of in vitro culturing an expandable neural progenitor or stem cell isolated from a mammalian central nervous system, and the culture itself, wherein said cell maintains its capability to differentiate into neurons, astrocytes, and oligodendrocytes and its ability to differentiate into oligodendrocyte-lineage cells efficiently. In addition, a method of treating a condition caused by a loss of myelin or a loss of oligodendrocytes is provided as is a composition comprising an isolated expandable neural stem cell or one cultured by the methods of the invention.
Owner:KIDO TSUNEO

Establishment method and application of clinical-grade neural stem cell line

InactiveCN103451153ALow tumorigenicityAvoid Tumor RiskNervous disorderMicroorganism based processesClinical gradeNeurulation
The invention belongs to the field of cytobiology and neurobiology, and provides an establishment method and application of a clinical-grade neural stem cell line. The method under GMP (Good Manufacturing Practice) control comprises two steps, namely, primary culturing of neural stem cells, and subculturing of neural stem cells. The identification result shows that the neural stem cell line established by the method can express a plurality of stem cell markers, can be differentiated into nerve cells, astrocyte and oligodendrocyte, has high stability, and is suitable for long-term culturing; a large amount of sufficient clinical-grade neural stem cells can be obtained by the method. The method can be used for establishing the neural stem cell line based on a plurality of neural stem cells and even single neural stem cell, and thus the error in study can be reduced as far as possible. By adopting the neural stem cell line established by the method and the cells obtained by differentiating the neural stem cell line, the problems of the safety and effectiveness risk in cell therapy of nervous system disease can be solved effectively, and safe and effective treatment on such diseases becomes possible.
Owner:栾佐

Method and application of induced neural stem cells

The invention discloses a method of induced neural stem cells and a composition. Through the method and the composition, peripheral blood mononuclear cells can be induced to form neural stem cells. The neural stem cells can express neural stem cell related genes, and can disintegrate out nerve cells, astroglia cells and oligodendrocyte. Dopaminergic nerve precursor cells formed through in vitro induction and disintegration of the neural stem cells are transplanted into the striatum corpora of a PD mouse model, no tumors are formed, the ethology of a mouse Parkinson 's disease model can be improved, and the process of the Parkinson 's disease can be delayed. The neural stem cell inducing and disintegrating method provided by the invention is simple and quick to operate, small in traumatic occlusion and good in safety, and is hopefully used for treating the Parkinson 's disease.
Owner:WISEHEART MEDICAL VALLEY CO LTD

Method for differentiation of embryonic stem cells into nerve cells through in vitro induction

The invention belongs to the field of biomedicine, and relates to a method for differentiation of embryonic stem cells into nerve cells through in vitro induction, especially to a method for induction of Olig2<+>-GFP<+>-mES nerve cell differentiation through a purine derivative Purmorphamine, and uses thereof. According to the present invention, an embryoid body mediated nerve induction method is adopted, Olig2-GFP<+>-mES is adopted as a cell model, a purine derivative Purmorphamine and all-trans retinoic acid are combined to carry out directed induction on the Olig2-GFP<+>-mES cells to obtain spinal motor neurons and oligodendrocyte progenitor cells through differentiation; experiment results show that the Purmorphamine can be used as a substitute of SHH, can effectively induce Olig2<+>-GFP<+>-mES differentiation to obtain high purity and function spinal motor neurons and oligodendroglial cells, and can cause expression changes of related genes; and transplant experiment results show that the induced nerve cells can promote partial function and morphology restoration after rat spinal cord injury, and have effects of spinal cord injury regeneration promotion and function reconstruction.
Owner:FUDAN UNIV

In Vitro Production Of Oligodendrocytes From Human Umbilical Cord Stem Cells

The invention provides a method of producing oligodendrocytes by in vitro differentiation of human multi-potent progenitor cells (MLPCs). The method comprises culturing isolated MLPCs on a first surface in a serum-free defined culture medium; replacing the culture medium with serum-free culture medium supplemented with bFGF, EGF and PDGF-AA for approximately 24 hours; changing the cultured MLPCs into the supplemented serum-free culture medium further supplemented with differentiation factors norepinephrine, forskolin. and K252a; establishing a 3D environment by covering the culture with a second surface opposite and spaced apart from the first surface, so as to contain the MLPCs therebetween; and continuing to culture until a majority of the MLPCs have differentiated into oligodendrocytes. Additionally included is a method of treatment for a subject afflicted by a disease characterized by central or peripheral nervous system demyelination, the method comprising transplanting into the subject oligodendrocytes produced according to the method disclosed.
Owner:UNIV OF CENT FLORIDA RES FOUND INC

Human IgM antibodies, and diagnostic and therapeutic uses thereof particularly in the central nervous system

Antibodies, and particularly human antibodies, are disclosed that demonstrate activity in the treatment of demyelinating diseases as well as other diseases of the central nervous system that are of viral, bacterial or idiopathic origin, including neural dysfunction caused by spinal cord injury. Neuromodulatory agents are set forth that include and comprise a material selected from the group consisting of an antibody capable of binding structures or cells in the central nervous system, a peptide analog, a hapten, active fragments thereof, agonists thereof, mimics thereof, monomers thereof and combinations thereof. The neuromodulatory agent has one or more of the following characteristics: it is capable of inducing remyelination; binding to neural tissue; promoting Ca−− signaling with oligodendrocytes; and promoting cellular proliferation of glial cells. Amino acid and DNA sequences of exemplary antibodies are disclosed. Methods are described for treating demyelinating diseases, and diseases of the central nervous system of humans and domestic animals, using polyclonal IgM antibodies and human monoclonal antibodies sHIgm22(LYM 22), sHIgm46(LYM46) ebvHIgM MSI19D10, CB2bG8, AKJR4, CB2iE12, CB2iE7, MSI19E5 and MSI10E10, active fragments thereof and the like. The invention also extends to the use of human antibodies, fragments, peptide derivatives and like materials, and their use in diagnostic and therapeutic applications, including screening assays for the discovery of additional antibodies that bind to cells of the nervous system, particularly oligodendrocytes.
Owner:MAYO FOUND FOR MEDICAL EDUCATION & RES

Transgenic zebrafish with specific myelin sheath removal capacity as well as preparation method and application thereof

The invention discloses transgenic zebrafish with specific myelin sheath removal capacity as well as a preparation method and an application thereof. The preparation method comprises the following steps: recombinant plasmid mbp-nfsB-EGFP and a Tol 2 transposase mRNA (messenger ribonucleic acid) are jointly introduced into wild zebrafish; and a zebrafish strain with stable inheritance is cultured and obtained, and the zebrafish strain with stable inheritance is the transgenic zebrafish with specific myelin sheath removal capacity, wherein the nucleotide sequence of an mbp gene promoter carried in the recombinant plasmid mbp-nfsB-EGFP is represented as SEQ ID NO.1. With adoption of the method, the transgenic zebrafish which can specifically remove myelin sheath, cause myelinoclasis and has stable inheritance capacity can be prepared and obtained effectively, under the action of metronidazole, oligodendrocyte of juvenile transgenic zebrafish can be removed specifically and the juvenile transgenic zebrafish has pathologic change of myelinoclasis together with decreased motor function, therefore, the transgenic zebrafish can provide a forceful animal model for a regulatory mechanism for viviperception of myelin sheath damage and regeneration and related drug screening.
Owner:GENERAL HOSPITAL OF PLA +1

Compounds and methods of promoting myelination

A method of promoting the generation of oligodendrocytes from oligodendrocyte precursor cells by enhancing their survival and / or maturation includes administering to the cell an effective amount of anagent that enhances and / or induces accumulation of delta8,9-unsaturated sterol intermediates of the cholesterol biosynthesis pathway in the oligodendrocyte precursor cells.
Owner:CASE WESTERN RESERVE UNIV

Application of hepatocyte growth factor in preparation of medicines for treating multiple sclerosis

The invention discloses an application of a hepatocyte growth factor (HGF) and a medulla desmohemoblast stem cell (MSC)-containing conditioned medium (MSC-CM) in preparation of medicines for treating multiple sclerosis (MS). The research result shows that in a cord sheath oligodendrocyte glycoprotein 35-55 peptide fragments (MOG35-55) induced MS animal model EAE, human MSC paracrine substance-containing MSC-CM is provided to reduce the EAE functional defect, and the growth of oligodendrocyte and neuron can be promoted when the functional cells are injured, wherein the HGF which is released by MSC performs the main effect; the effect provided to HGF is similar with MSC-CM, and the growth and movement of oligodendrocyte and neuron can be stimulated, the cord sheath restoration capability can be enhanced, the pathology load of MS animal model (EAE) can be minimized, the disease recovery function can be promoted, and short HGF treatment on the EAE model is provided for continuously guiding the disease function improvement. According to the invention, the application scope of HGF and MSC-CM can be widened, an effective medicine is provided for clinical treatment of MS, and the deep exploitation of a novel high efficiency MS treatment medicine can be realized simultaneously.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA

Combination therapies and uses for treatment of demyelinating disorders

InactiveUS20150238602A1Enhances myelinationPromotes oligodendrocyte numberOrganic active ingredientsSenses disorderDemyelinating DisorderMyelin body formation
Methods and compositions for enhancing one or more of: myelination, re-myelination, oligodendrocyte numbers, or neuroaxonal protection, while ameliorating an inflammatory condition in a human subject are disclosed. In certain embodiments, the methods and compositions described herein include a reparative agent (e.g., a LINGO-1 antagonist) and an immunomodulatory agent, in combination. Thus, methods, compositions and kits described herein can be useful for treating a CNS demyelinating disease.
Owner:BIOGEN MA INC

Preparation method of human oligodendrocyte precursor cell inhibiting nerve secondary injury, kit and application thereof

ActiveCN106011173AInhibition of secondary damageProtect physiological functionNervous disorderNeuregulinsVirus typeGlial Growth Factor
The invention provides a preparation method of a human oligodendrocyte precursor cell inhibiting nerve secondary. Specifically, the method includes: constructing a virus expression vector of two glial growth factors, conducting cotransfection of a human oligodendrocyte precursor cell to prepare the human oligodendrocyte precursor cell, which can achieve high expression of the two glial growth factors, i.e. a growth related factor 43 and a glial growth factor 2, give play to the combined action of the two to promote oligodendrocyte repair, and inhibit nerve secondary injury caused by proliferative response and glial scar formation after oligodendrocyte injury. Also, the proliferation and differentiation ability of oligodendrocyte precursor cell itself is also utilized to participate in nerve function repair, and no tumorigenesis exists. The invention also provides a kit for preparation of the human oligodendrocyte precursor cell inhibiting nerve secondary. The human oligodendrocyte precursor cell provided by the invention has very good therapeutic effect on nervous system diseases.
Owner:江西美奥生物技术有限公司

Improvements in Oligodendroglial Cell Culturing Methods and in Methods for Treating Neurodegenerative Disorders by Using Thyroid Hormones or Analogues

The present invention relates to methods of treating or ameliorating certain neurodegenerative disorders (namely, dysmyelinating and demyelinating disorders) in patients in need of such treatment or amelioration. The invention provides methods of treating or ameliorating a patient in need of such treatment and includes the administration to the patient of: (a) thyroid hormones or thyroid hormone analogues; (b) cell replacement therapies involving the use of homogenous Oligodendrocyte Precursor Cells derived from embryonic stem cells that have been treated with thyroid hormones or thyroid hormone analogues; (c) gene therapy to correct mutated genes in vivo; or (d) a combination of two or more of (a), (b) and (c). The invention also provides compositions and formulations of thyroid hormones and thyroid hormone analogues for use in treating or ameliorating such disorders.
Owner:NEUORPHAN PTY LTD

Differentiation and amplification method for inducing human neural stem/progenitor cells to differentiate into oligodendrocyte progenitor cells and application thereof

A Method for inducing human neural stem / progenitor cells to differentiate into oligodendrocyte progenitor cells and application thereof comprises following steps of: pre-treating neural stem cells derived from different resources in pre-treatment medium including bFGF and EGF for culturing; and inducing neural stem cells after pre-treating with inducing medium including PDGF-AA, bFGF and NT3, so as to differentiate into oligodendrocyte progenitor cells (OPCs). Main markers of the OPCs obtained by the method, such as NG2, O4, A2B5 and PDGFR, have a positive rate of 80˜90%. The OPCs obtained thereby is capable of proliferating steadily in the OPCs inducing medium for at least 10 generations and simultaneously maintaining biological characteristics thereof unchanged. The OPCs induced by the present invention can be applied in treating myelin-associated diseases or researching on drug screening.
Owner:LUAN ZUO

Assay to measure the levels of circulating demethylated DNA

A method for measuring blood levels of DNA that is released upon death from specialized cells in the body, by using PCR or a quantitative probe technology to detect amplified methylated and demethylated forms of cell-specific gene DNA, representing normal tissue and cell specific origin, respectively. Using probes permits the sensitive and specific identification of demethylated cell-specific DNA patterns that are present only in the dying cells. The method offers a bioassay for detecting β cell loss in diabetes based on circulating demethylated insulin gene DNA, and circulating demethylated myelin oligodendrocyte protein (MOG) genes of oligodendrocytes in multiple sclerosis, for example, and may be useful for screening, monitoring of disease progression, and selection and monitoring of therapies.
Owner:NYU WINTHROP HOSPITAL

Construction method and application of schizophrenia animal model based on central nervous system myelin function change

The invention discloses a construction method and application of a schizophrenia animal model based on central nervous system myelin function change, and belongs to the technical field of biological medicine. The important effect of oligodendrocyte / myelin dysfunction in incidence of schizophrenia is used as a study breakthrough point, according to the central nervous system oligodendrocyte / myelindysfunction, the behavioral phenotype is similar to the animal behavior of typical schizophrenia, and the schizophrenia animal model is constructed and belongs to a brand-new pathogenesis mechanism for studying schizophrenia. Since animal model construction is derived from mating of genetically modified mice, a large number of model mice can be obtained, and the repeatability between experimentalbatches and the stability of experimental results are ensured. Experimental study on the constructed animal model is carried out through a histological method and a behavioral method, and it is foundthat the animal model can provide an important research basis for comprehensively analyzing and studying the schizophrenia occurrence mechanism and clinical treatment strategies.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
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