Establishment method and application of clinical-grade neural stem cell line

Abstract

The invention belongs to the field of cytobiology and neurobiology, and provides an establishment method and application of a clinical-grade neural stem cell line. The method under GMP (Good Manufacturing Practice) control comprises two steps, namely, primary culturing of neural stem cells, and subculturing of neural stem cells. The identification result shows that the neural stem cell line established by the method can express a plurality of stem cell markers, can be differentiated into nerve cells, astrocyte and oligodendrocyte, has high stability, and is suitable for long-term culturing; a large amount of sufficient clinical-grade neural stem cells can be obtained by the method. The method can be used for establishing the neural stem cell line based on a plurality of neural stem cells and even single neural stem cell, and thus the error in study can be reduced as far as possible. By adopting the neural stem cell line established by the method and the cells obtained by differentiating the neural stem cell line, the problems of the safety and effectiveness risk in cell therapy of nervous system disease can be solved effectively, and safe and effective treatment on such diseases becomes possible.

Description

technical field [0001] The invention belongs to the fields of cell biology and neurobiology, and in particular relates to a method for establishing a neural stem cell line and its use. Background technique [0002] Central nervous system damage was once considered irreparable, and traditional medicine and other treatment methods also focused on maintaining the survival and function of existing nerve cells. In recent years, with the research and development of stem cell technology, researchers have found that nerve cells lost during nervous system damage can be restored by transplanting stem cells, and this method is very effective. Neural stem cells (NSCs) are a kind of cells with self-renewal ability and multi-directional differentiation potential. Since NSCs are derived from the nervous system and are natural neuroblasts, neural stem cell transplantation is the most ideal for treating nervous system injury diseases choose. NSCs can differentiate into various nerve cells ...

AI Technical Summary

Problems solved by technology

[0004] In addition, due to the low monoclonal formation ability of neural stem cells, it is difficult to cultivate neural stem cell spheres from one neural stem cell in the usual method, and the cell spheres formed after culturing multiple neural stem cells are derived from multiple cells, and the initial cells are not uniform. Greater resistance, which increases the difficulty for subsequent basic or clinical research

[0005] The establishment of a stable neural stem cell line will help us further research on NSCs transplantation for the treatment of nervous system diseases, but the establishment of the neural stem cell line reported so far is based on transgenic technology, that is, immortalization-related genes such as telomerase gene or Proto-oncogenes are stably transfected into NSCs to obtain NSCs that can be cultured for a long time. Transgenic technology may change some biological characteristics of NSCs, and even cause tumorigenicity, which brings risks and variables to research.

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