Establishment method and application of clinical-grade neural stem cell line

A technology of neural stem cells and establishment methods, applied in nervous system cells, nervous system diseases, microbial-based methods, etc., can solve problems such as research risks and variables, tumorigenicity, large cell heterogeneity, etc., and reduce cell differentiation problems, reduce research errors, and ensure stability

Inactive Publication Date: 2013-12-18
栾佐
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, due to the low monoclonal formation ability of neural stem cells, it is difficult to cultivate neural stem cell spheres from one neural stem cell in the usual method, and the cell spheres formed after culturing multiple neural stem cells are derived from multiple cells, and the initial cells are not uniform. Greater resistance, which increases the difficulty for subsequent basic or clinical research
[0005] The establishment of a stable neural stem cell line will help us furthe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishment method and application of clinical-grade neural stem cell line
  • Establishment method and application of clinical-grade neural stem cell line
  • Establishment method and application of clinical-grade neural stem cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Various liquids used in the present embodiment are illustrated as follows:

[0057] 1. Basal medium: composed of DMEM, F12, HEPES and D-glucose. Wherein, the ratio of DMEM and F12 is volume ratio 1:1-3:1, such as 3:1; HEPES concentration 10-20mmol / L, such as 15mmol / L; D-glucose concentration 1-2g / 100ml, such as 1.5g / L 100ml.

[0058] 2. Primary culture medium for neural stem cells: consisting of basal medium, B27, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), transferrin, progesterone, putrescine , sodium selenate, insulin and heparin. Among them, B27 is 1×; EGF concentration is 15-25ng / ml, such as 20ng / ml; bFGF concentration is 15-25ng / ml, such as 20ng / ml; LIF concentration is 7-13ng / ml, such as 10ng / ml; transferrin concentration 50-150μg / ml, such as 100μg / ml; progesterone 10-30nmol / L, such as 20nmol / L; putrescine 50-150μmol / L, such as 100μmol / L; sodium selenate 20-40nmol / L, such as 30nmol / L ; Insulin 10-49 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of cytobiology and neurobiology, and provides an establishment method and application of a clinical-grade neural stem cell line. The method under GMP (Good Manufacturing Practice) control comprises two steps, namely, primary culturing of neural stem cells, and subculturing of neural stem cells. The identification result shows that the neural stem cell line established by the method can express a plurality of stem cell markers, can be differentiated into nerve cells, astrocyte and oligodendrocyte, has high stability, and is suitable for long-term culturing; a large amount of sufficient clinical-grade neural stem cells can be obtained by the method. The method can be used for establishing the neural stem cell line based on a plurality of neural stem cells and even single neural stem cell, and thus the error in study can be reduced as far as possible. By adopting the neural stem cell line established by the method and the cells obtained by differentiating the neural stem cell line, the problems of the safety and effectiveness risk in cell therapy of nervous system disease can be solved effectively, and safe and effective treatment on such diseases becomes possible.

Description

technical field [0001] The invention belongs to the fields of cell biology and neurobiology, and in particular relates to a method for establishing a neural stem cell line and its use. Background technique [0002] Central nervous system damage was once considered irreparable, and traditional medicine and other treatment methods also focused on maintaining the survival and function of existing nerve cells. In recent years, with the research and development of stem cell technology, researchers have found that nerve cells lost during nervous system damage can be restored by transplanting stem cells, and this method is very effective. Neural stem cells (NSCs) are a kind of cells with self-renewal ability and multi-directional differentiation potential. Since NSCs are derived from the nervous system and are natural neuroblasts, neural stem cell transplantation is the most ideal for treating nervous system injury diseases choose. NSCs can differentiate into various nerve cells ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/0797A61K35/30A61P25/00C12R1/91
Inventor 栾佐
Owner 栾佐
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap