Method for differentiation of embryonic stem cells into nerve cells through in vitro induction

A technology for embryonic stem cells and cell differentiation, which is applied in the neural cell differentiation of Olig2+-GFP+-mES induced by purine derivatives, neural cell induction, and in vitro induction of embryonic stem cells into the field of neural cells, to promote recovery, improve recovery, and promote myeloid Effects of sheath regeneration and recovery of nerve function
CN102899285AInactive Publication Date: 2013-01-30FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUDAN UNIV
Publication Date
2013-01-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of biomedicine, and relates to a method for differentiation of embryonic stem cells into nerve cells through in vitro induction, especially to a method for induction of Olig2<+>-GFP<+>-mES nerve cell differentiation through a purine derivative Purmorphamine, and uses thereof. According to the present invention, an embryoid body mediated nerve induction method is adopted, Olig2-GFP<+>-mES is adopted as a cell model, a purine derivative Purmorphamine and all-trans retinoic acid are combined to carry out directed induction on the Olig2-GFP<+>-mES cells to obtain spinal motor neurons and oligodendrocyte progenitor cells through differentiation; experiment results show that the Purmorphamine can be used as a substitute of SHH, can effectively induce Olig2<+>-GFP<+>-mES differentiation to obtain high purity and function spinal motor neurons and oligodendroglial cells, and can cause expression changes of related genes; and transplant experiment results show that the induced nerve cells can promote partial function and morphology restoration after rat spinal cord injury, and have effects of spinal cord injury regeneration promotion and function reconstruction.
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Description

technical field

[0001] The invention belongs to the field of biomedicine and relates to a method for inducing nerve cells, in particular to a method for inducing differentiation of embryonic stem cells into nerve cells in vitro, and in particular to purine derivatives (Purmorphamine) inducing Olig2 + -GFP + - A method for neural cell differentiation of mES and its use. Background technique

[0002] Spinal cord injury (SCI) is one of the common clinical traumatic diseases. The occurrence of SCI is mostly caused by traffic injuries, falling injuries or sports injuries, etc., and it has been on the rise in recent years. Once SCI is formed, it often leads to severe behavioral dysfunction and brings great burden to patients, families and society. In recent years, regenerative medicine has brought new hope to the treatment of spinal cord injury. Embryonic stem cells (ESCs), with their strong ability to proliferate and differentiate, can be induced to differentiate into neurons...

Claims

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