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Application of hepatocyte growth factor in preparation of medicines for treating multiple sclerosis

A technology of hepatocyte growth factor and multiple sclerosis, applied in the field of pharmacy and medicine, to achieve the effect of broadening the scope of application

Inactive Publication Date: 2012-10-10
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no literature report on the relationship between HGF and MS

Method used

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  • Application of hepatocyte growth factor in preparation of medicines for treating multiple sclerosis
  • Application of hepatocyte growth factor in preparation of medicines for treating multiple sclerosis
  • Application of hepatocyte growth factor in preparation of medicines for treating multiple sclerosis

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Embodiment Construction

[0029] The therapeutic action and effect of HGF on MS will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition.

[0030] The MSC-CM described in the preferred embodiment is made by the following method: get 20ml of normal human bone marrow, add 25ml of DMEM low-sugar medium containing 10% (v / v) calf serum and 1000mg / L D-glucose, 2000rpm Centrifuge for 5 minutes, remove the supernatant and count the cells according to every 2 x 10 8 The ratio of adding 5ml lymphocyte separation medium to each cell was added to the lymphocyte separation medium, centrifuged at 480g for 15 minutes, took the second layer of lymphocytes (about 10-14ml) into a 50ml centrifuge tube, and added 10% (v / v) calf Serum and 1000mg / L D-glucose DMEM low-glucose medium 50ml, mixed completely, centrifuged at 450g for 5 minutes, removed the supernatant and counted ce...

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Abstract

The invention discloses an application of a hepatocyte growth factor (HGF) and a medulla desmohemoblast stem cell (MSC)-containing conditioned medium (MSC-CM) in preparation of medicines for treating multiple sclerosis (MS). The research result shows that in a cord sheath oligodendrocyte glycoprotein 35-55 peptide fragments (MOG35-55) induced MS animal model EAE, human MSC paracrine substance-containing MSC-CM is provided to reduce the EAE functional defect, and the growth of oligodendrocyte and neuron can be promoted when the functional cells are injured, wherein the HGF which is released by MSC performs the main effect; the effect provided to HGF is similar with MSC-CM, and the growth and movement of oligodendrocyte and neuron can be stimulated, the cord sheath restoration capability can be enhanced, the pathology load of MS animal model (EAE) can be minimized, the disease recovery function can be promoted, and short HGF treatment on the EAE model is provided for continuously guiding the disease function improvement. According to the invention, the application scope of HGF and MSC-CM can be widened, an effective medicine is provided for clinical treatment of MS, and the deep exploitation of a novel high efficiency MS treatment medicine can be realized simultaneously.

Description

Technical field [0001] The invention belongs to the field of medicine and relates to the new use of hepatocyte growth factor in the pharmaceutical field. Background technique [0002] Multiple sclerosis (MS) is a demyelinating disease of the central nervous system that mainly affects young and middle-aged people (20-50 years old) in their prime. There are more than 2,500,000 MS patients in the world. The number of MS patients in my country was about 65,000 in 2004, and has shown a rapid growth trend in recent years. The exact cause of MS is still unknown. It is currently believed to be related to autoimmunity. Its clinical manifestations include extreme fatigue, double vision, loss of sexual function, numbness of limbs, difficulty walking, paralysis, etc. The biggest difference from other neurological diseases is the recurrence of initial clinical symptoms. relapsing-remitting,” frequently promoting greater loss of function. At present, there is no radical cure for MS in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61P25/00
Inventor 白莲花
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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