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45 results about "Spherical cell" patented technology
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Ceramic molded body and metal matrix composite
InactiveUS20060057356A1Improve reducibilityReduce volume ratioLayered productsCeramic shaping apparatusPorosityCeramic molding
The ceramic molded body of the present invention includes spherical cells of a spherical bubble plurally formed therein: in the ceramic molded body the spherical cells neighboring each other are communicated through communication porosities and form a three-dimensional network structure, and a ratio (Md / MD) of a median (Md) of inner diameters of the communication porosities to a median (MD) of inner diameters of the spherical cells is less than 0.5. In the ceramic molded body used for manufacturing a metal matrix composite, a metal is filled within the spherical cells and the communication porosities.
Owner:HONDA MOTOR CO LTD
Process for producing polyurethane foam
InactiveUS8314029B2High thickness accuracyAir can be trappedOther chemical processesDecorative surface effectsCarbamateSpherical cell
A method for manufacturing a polishing pad containing substantially spherical cells and having high thickness accuracy includes preparing a cell-dispersed urethane composition by a mechanical foaming method; continuously discharging the cell-dispersed urethane composition from a single discharge port to a substantially central portion in the width direction of a face material A, while feeding the face material A; laminating a face material B on the cell-dispersed urethane composition; then uniformly adjusting the thickness of the cell-dispersed urethane composition by thickness adjusting means; curing the cell-dispersed urethane composition with the thickness adjusted in the preceding step without applying any additional load to the composition so that a polishing sheet including a polyurethane foam is formed; and cutting the polishing sheet.
Owner:ROHM & HAAS ELECTRONICS MATERIALS CMP HLDG INC
Polishing pad and method for producing same
ActiveCN103930975AImprove grinding uniformityPrevent penetrationSemiconductor/solid-state device manufacturingLapping toolsEngineeringTan delta
Provided are: a polishing pad, which improves the problem of scratches that are produced in cases where conventional hard (dry) polishing pads are used, which has having excellent polishing rate and polishing uniformity, and which is applicable not only to primary polishing but also to final polishing; and a method for producing the polishing pad. A polishing pad for semiconductor device polishing, which comprises a polishing layer having a polyurethane polyurea resin molded body that contains generally spherical cells. This polishing pad for semiconductor device polishing is characterized in that: the polyurethane polyurea resin molded body has a closed cell ratio of 60-98%; the ratio of the loss modulus (E") to the storage modulus (E'), namely loss modulus / storage modulus (tan delta) of the polyurethane polyurea resin molded body is 0.15-0.30; the storage modulus (E') is 1-100 MPa; and the polyurethane polyurea resin molded body has a density (D) of 0.4-0.8 g / cm<3>.
Owner:FUJIBO HLDG
Cylinder block
InactiveUS7073476B2Increase volumeReduce volume fractionCasingsCylinder headsCylinder blockMetal matrix composite
Owner:HONDA MOTOR CO LTD
Ceramic molded body and metal matrix composite
InactiveUS7357976B2Improve reducibilityReduce volume ratioLayered productsCeramic shaping apparatusPorosityCeramic molding
The ceramic molded body of the present invention includes spherical cells of a spherical bubble plurally formed therein: in the ceramic molded body the spherical cells neighboring each other are communicated through communication porosities and form a three-dimensional network structure, and a ratio (Md / MD) of a median (Md) of inner diameters of the communication porosities to a median (MD) of inner diameters of the spherical cells is less than 0.5. In the ceramic molded body used for manufacturing a metal matrix composite, a metal is filled within the spherical cells and the communication porosities.
Owner:HONDA MOTOR CO LTD
Method for growing cell embryos of Dendrobium huoshanense
InactiveCN102246697AAchieve continuous useEffective protectionPlant tissue cultureHorticulture methodsBiotechnologyExogenous hormones
The invention relates to a method for growing cell embryos of Dendrobium huoshanense, which comprises the following operating steps: (1) inducing sterile in-vitro cuttings; (2) inducing embryonic calli; (3) inducing somatic embryos; and (4) performing synchronous propagation of somatic embryos to obtain a large amount of cell embryos of Dendrobium huoshanense. In the method, morphological lower ends of the jointed stem segments of the sterile in-vitro cuttings are used as explants, the oxidation-reduction potential of the induction culture medium is controlled, exogenous hormone is not required to be added to induce the embryonic calli of Dendrobium huoshanensem, and the exogenous hormone is prevented from being enriched into complete plants through somatic cells and endangering health of human body. By controlling ambient humidity, embryonic calla are grown into somatic embryos, the consistency of spherical cell embryos is over 90 percent, and the survival rate of regenerated plants is over 85 percent. In the invention, the process is simple and not limited by time and seasons, and industrial automatic production of g cell embryos of Dendrobium huoshanense and in-vitro cuttings of Dendrobium huoshanense can be carried out in door for satisfying needs for production and planting.
Owner:芜湖华信生物药业股份有限公司
Foam, production method for foam, and functional foam
InactiveUS20130224467A1Improve heat resistanceImprove toughnessSynthetic resin layered productsAnimal housingHeat resistanceCrack free
Provided are a novel foam which has a uniform fine-cell structure and is excellent in toughness and heat resistance, and a production method therefor. Also provided is a functional foam which includes the above-mentioned foam and has imparted thereto various functions. The foam includes spherical cells, in which: the spherical cells each have an average pore diameter of less than 20 μm; the foam has a density of 0.15 g / cm3 to 0.9 g / cm3; and the foam is crack-free in a 180° bending test. The functional foam includes the foam.
Owner:NITTO DENKO CORP
Fixing-unit roller, fixing unit, and image forming apparatus
A fixing-unit roller includes: a core bar; and an elastic layer formed on an outer peripheral surface of the core bar. The elastic layer is formed from a porous material that contains a plurality of cells. Cells in a cross section obtained by cutting across the porous material are 0.1 μm or greater and 50 μm or less in size. A ratio of an area occupied by composite cells, which are made of partially-overlapping spherical cells, in a 200-μm square in the cross section to an area of the square is 60% or greater and 70% or less.
Owner:RICOH KK
Composite sheet
InactiveUS20130210301A1Low costUniform microporous structureSynthetic resin layered productsWoven fabricsHeat resistanceVolumetric Mass Density
Provided is a novel composite sheet, including a substrate and a foamed layer which is provided on at least one surface side of the substrate, at a low cost in an environment-friendly manner, in which the composite sheet includes a foamed layer having a uniform fine-cell structure, the average pore diameter of each of spherical cells of the foamed layer can be precisely controlled to a small one, the control range of the density of the foamed layer is wide, the control range of the thickness of the foamed layer is wide, and the composite sheet can express an excellent mechanical strength and is preferably excellent in toughness and heat resistance. The composite sheet includes a substrate; and a foamed layer which is provided on at least one surface side of the substrate, in which: the foamed layer has spherical cells, an average pore diameter of each of the spherical cells being less than 30 μm; and the foamed layer has a density of 0.1 g / cm3 to 0.9 g / cm3.
Owner:NITTO DENKO CORP
Cylinder block
InactiveUS20050279315A1Improve thermal conductivityImprove rigidityCasingsCylinder headsCylinder blockMetal matrix composite
In a cylinder block, at least one member disposed around a bore is made of a metal matrix composite including a ceramic compact having a three-dimensional mesh structure comprised of a plurality of spherical cells and a plurality of communicating pores for allowing adjacent spherical cells to communicate with each other, with the plurality of spherical cells filled with a metal.
Owner:HONDA MOTOR CO LTD
W/o emulsion, foam, and functional foam
InactiveUS20130216814A1Good emulsifying effectGood storage stabilityAnimal housingThin material handlingOil phasePore diameter
Provided is a W / O emulsion, including a continuous oil phase component and an aqueous phase component immiscible with the continuous oil phase component, in which: the continuous oil phase component includes a hydrophilic polyurethane-based polymer and an ethylenically unsaturated monomer; the hydrophilic polyurethane-based polymer includes a polyoxyethylene polyoxypropylene unit derived from polyoxyethylene polyoxypropylene glycol; and the polyoxyethylene polyoxypropylene unit includes 5 wt % to 25 wt % of polyoxyethylene. Also provided is a foam, including a hydrophilic polyurethane-based polymer, in which: the foam has an open-cell structure in which through-holes are present between adjacent spherical cells; the spherical cells each have an average pore diameter of less than 20 μm; the through-holes each have an average pore diameter of 5 μm or less; and the foam has surface openings each having an average pore diameter of 20 μm or less in a surface thereof.
Owner:NITTO DENKO CORP
Polishing pad and manufacturing method therefor
InactiveCN103608903ASuppress strong pressureReduce wearAbrasion apparatusSemiconductor/solid-state device manufacturingYoung's modulusPolyurea
Provided are a polishing pad which remedies the problem of scratches occurring when a conventional hard (dry) polishing pad is used, which is excellent in polishing rate and polishing uniformity, and which can be used for not only primary polishing but also finish polishing, and a manufacturing method therefor. The polishing pad is a polishing pad for polishing a semiconductor device, comprising a polishing layer having a polyurethane-polyurea resin foam containing substantially spherical cells, wherein the polyurethane-polyurea resin foam has a Young's modulus E in a range from 450 to 30000 kPa, and a density D in a range from 0.30 to 0.60 g / cm 3 .
Owner:FUJIBO HLDG
Polishing pad and manufacturing method therefor
ActiveUS20140038503A1Increase ratingsImprove uniformityAbrasion apparatusSemiconductor/solid-state device manufacturingVolumetric Mass DensityEngineering
A polishing pad for polishing a semiconductor device and manufacturing method therefor, the polishing pad comprising a polishing layer having a polyurethane-polyurea resin foam containing substantially spherical cells, wherein an M-component of the polyurethane-polyurea resin foam has a spin-spin relaxation time T2 of 160 to 260 μs, the polyurethane-polyurea resin foam has a storage elastic modulus E′ of 1 to 30 MPa, the storage elastic modulus E′ being measured at 40° C. with an initial load of 10 g, a strain range of 0.01 to 4%, and a measuring frequency of 0.2 Hz in a tensile mode, and the polyurethane-polyurea resin foam has a density D in a range from 0.30 to 0.60 g / cm3. The polishing pad remedies the problem of scratches occurring when a conventional hard (dry) polishing pad is used, which is excellent in polishing rate and polishing uniformity, and can be used for primary polishing or finish polishing.
Owner:FUJIBO HLDG
Polishing pad and manufacturing method therefor
ActiveUS20140033615A1Increase ratingsImprove uniformityAbrasion apparatusSemiconductor/solid-state device manufacturingMetallurgyVolumetric Mass Density
Provided are a polishing pad which remedies the problem of scratches occurring when a conventional hard (dry) polishing pad is used, which is excellent in polishing rate and polishing uniformity, and which can be used for not only primary polishing but also finish polishing, and a manufacturing method therefor. The polishing pad is a polishing pad for polishing a semiconductor device, comprising a polishing layer having a polyurethane-polyurea resin foam containing substantially spherical cells, wherein the polyurethane-polyurea resin foam has a hard segment content (HSC) in a range from 26 to 34%, and has a density D in a range from 0.30 to 0.60 g / cm3, the hard segment content (HSC) being determined by the following formula (1):HSC=100×(r−1)×(Mdi+Mda)÷(Mg+r×Mdi+(r−1)×Mda) (1).
Owner:FUJIBO HLDG
Process for producing ceramic molding having three-dimensional network structure
InactiveUS20050121816A1Improve uniformityEasy to adjustCeramic shaping apparatusCeramicwareCeramic moldingNetwork structure
A process for producing a ceramic molding having a three-dimensional network structure with a plurality of spherical cells and a plurality of through holes present in dividing walls between the spherical cells, includes: a step of obtaining an aggregate of coated particles in which a plurality of ceramic particles are attached to the surface of spherical synthetic resin particles; a step of arranging the aggregate of coated particles in closest packed form within a mold; a step of filling gaps in the aggregate of coated particles with a ceramic so as to give a shaped product; a step of releasing the shaped product from the mold; a step of thermally decomposing the spherical synthetic resin particles of the shaped product so as to form the spherical cells and form the plurality of through holes in dividing wall-forming sections that are present between adjacent spherical cells; and a step of sintering the ceramic particles and the ceramic so as to form the dividing walls.
Owner:HONDA MOTOR CO LTD
Method of non-exogenous induction of pluripotent stem cell to be GABA (Gamma Amino Acid Butyric Acid) neuron and application
InactiveCN104862279ANo distractionLow differentiation efficiencyNervous disorderNervous system cellsMatrigelInduced pluripotent stem cell
The invention discloses a method of non-exogenous induction of a pluripotent stem cell to be a GABA neuron. The method includes the following steps of 1 cultivating a pluripotent stem cell in a Matrigel or a Vitronectin matrigel system to the density of 60 % -80 %; 2 digesting the pluripotent stem cell obtained in step 1 with Dispase or EDTA (Ethylene Diamine Tetraacetic Acid), differentiating the suspension culture through neural induction on that day, and recording the day as the 0 day; 3 adding neural induction differentiation solution for further cultivation when a spherical cell formed by the pluripotent stem cell can be observed; 4 performing adherence on the seventh day; 5 adding SHH or a small molecule compound Purmophine on the tenth day for continuous induction; 6 blowing off a formed neural tube structure on the sixteenth day and continuing to cultivate with the neural induction solution; 7 obtaining the GABA neuron on the twenty-second day. The method of non-exogenous induction of the pluripotent stem cell to be the GABA neuron can significantly improve cultivation solution complication and low differentiation rate and eliminate the interference of exogenous factors.
Owner:NANJING MEDICAL UNIV
Microsphere with multiple magnetic nano cores@gaps@porous shell structures and preparation method thereof
InactiveCN104511263ASimple preparation processLow priceFerroso-ferric oxidesNanotechnologyCell wallReagent
The invention relates to a microsphere with multiple magnetic nano cores@gaps@porous shell structures and a preparation method thereof. The microsphere structure is characterized in that a porous hollow shell contains a plurality of magnetic nano cores. In the preparation method, iron (III) is taken as the single iron precursor, the spherical cell wall of unicellular algae is taken as the precursor of porous microsphere wall, the substances in the algae cells are used to replace chemical reagents to carry out reactions, and a hydrothermal method with mild conditions is performed to prepare the microsphere with multiple magnetic nano cores@gaps@porous shell structures. The preparation method is simple; a microsphere, which has a complicated structure composed of a porous hollow shell and a plurality of magnetic cores, can be produced without a template; the preparation process is green and environment-friendly; no organic reagent is added, and the obtained microsphere has the advantages of excellent magnet-response performance and large loading space.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Microcavity impedance sensor for real-time monitoring of activity and proliferation ability of 3D cells and preparation method
ActiveCN109628291AMonitor activity in real timeReal-time monitoring of proliferative capacityBioreactor/fermenter combinationsBiological substance pretreatmentsMicro nanoApoptosis
The invention discloses a 3D microcavity impedance sensor for real-time monitoring of activity and proliferation ability of cells and preparation method. The 3D microcavity impedance sensor is firstlymanufactured by adopting a micro-nano processing technology; 3D spherical cell culture is conducted on tumor cells; 3D spherical cells are inoculated into a trapezoidal microgroove structure in the microcavity impedance sensor, are attached to counter electrodes on the side wall of the microgroove and cause the reduction of the transfer efficiency of electrons on the electrode surfaces, so that the impedance values of the electrodes rise and are increased with diameter increase of proliferation spheres of the 3D spherical cell, and after an anti-tumor drug acts on the 3D spherical cells to cause apoptosis, the impedance values of the electrodes are decreased, and the activity and proliferation ability of the 3D spherical cells are monitored by calculating the change rate of the impedancevalues of the 3D spherical cells. The constructed microcavity impedance sensor can monitor the activity and proliferation ability of 3D spherical cells with high throughput in real time for a long time.
Owner:ZHEJIANG UNIV
High-stability DHA algal oil preparing method
InactiveCN105779518ASimple operation processEasy to operateMicroorganism based processesFermentationNutritive valuesPlant hormone
The invention relates to a high-stability DHA algal oil preparing method and belongs to the technical field of health care products. The method aims to solve the problems of the prior art that to improve the antioxidant ability of DHA, artificially synthesized antioxidant harmful to the health of people is added, matter with odor and other micromolecule impurities can not be effectively removed during deodorization, and therefore the sensor quality, nutritive value and oxidization stability of refined DHA algal oil are affected and DHA in algal oil is lost after deodorization. According to the method, chlorococcum is activated with plant hormones so that growth of chlorococcum can be promoted and in vivo grease can be increased, then chlorococcum is mixed with natural plants for mixed fermentation, deodorization is conducted under the action of the natural plants, antioxidant ability is improved at the same time, then chlorococcum spherical cells are damaged through bacterium enzyme rich in biogas slurry, DHA algal oil is completely released through high-pressure blasting, and finally ethyl alcohol refrigeration and bentonite decoloration are conducted to obtain the high-stability DHA algal oil.
Owner:CHANGZHOU DAAO NEW MATERIAL TECH CO LTD
Process for producing ceramic molding having three-dimensional network structure
InactiveUS7556755B2Improve uniformityEasy to adjustCeramic shaping apparatusCeramicwareCeramic moldingNetwork structure
A process for producing a ceramic molding having a three-dimensional network structure with a plurality of spherical cells and a plurality of through holes present in dividing walls between the spherical cells, includes: a step of obtaining an aggregate of coated particles in which a plurality of ceramic particles are attached to the surface of spherical synthetic resin particles; a step of arranging the aggregate of coated particles in closest packed form within a mold; a step of filling gaps in the aggregate of coated particles with a ceramic so as to give a shaped product; a step of releasing the shaped product from the mold; a step of thermally decomposing the spherical synthetic resin particles of the shaped product so as to form the spherical cells and form the plurality of through holes in dividing wall-forming sections that are present between adjacent spherical cells; and a step of sintering the ceramic particles and the ceramic so as to form the dividing walls.
Owner:HONDA MOTOR CO LTD
Polishing pad and manufacturing method therefor
ActiveUS9011212B2Increase ratingsImprove uniformityAbrasion apparatusSemiconductor/solid-state device manufacturingYoung's modulusEngineering
Provided are a polishing pad which remedies the problem of scratches occurring when a conventional hard (dry) polishing pad is used, which is excellent in polishing rate and polishing uniformity, and which can be used for not only primary polishing but also finish polishing, and a manufacturing method therefor. The polishing pad is a polishing pad for polishing a semiconductor device, comprising a polishing layer having a polyurethane-polyurea resin foam containing substantially spherical cells, wherein the polyurethane-polyurea resin foam has a Young's modulus E in a range from 450 to 30000 kPa, and a density D in a range from 0.30 to 0.60 g / cm3.
Owner:FUJIBO HLDG
Culture method of breast cancer MCF-7 cell stem cell levitated spheres
InactiveCN106676073ADifferent shapesCell culture active agentsTumor/cancer cellsPenicillinSingle cell suspension
The invention discloses a culture method of breast cancer MCF-7 cell stem cell levitated spheres. The culture method comprises the following steps: 1) collecting human breast cancer MCF-7 cells at a logarithmic growth phase, paving the collected cells on a 75 to 83 percent culture bottle, dissociating the cells with 0.25 percent by mass pancreatin till the cells become round, and discarding the pancreatin; 2) adding a DMEM / H-G complete culture solution to end a reaction, blowing and beating with a dropper to form a single-cell suspension, centrifuging at the speed of 1,000 revolutions per minute for 5 minutes, discarding supernatant, adjusting the cell concentration, and performing passage in different bottles in the ratio of 1 to 3; 3) re-suspending the MCF-7 cells subjected to passage in a DMEM / F-12 culture medium which does not contain serum, adjusting the cell concentration to 0.5*10<5> / mL, putting into a penicillin glass bottle, covering with a bottle plug, forming an oxygen-lack environment, culturing at the temperature of 37 DEG C in 5% CO2 without substituting the culture medium, mechanically blowing and beating into a single-cell suspension after a microcapsule is formed, and performing conventional passage. The breast cancer MCF-7 cell stem cell levitated spheres are cultured by an oxygen-free culture method, and spherical cell clusters remarkably growing in a suspending way appear on the second day of culture.
Owner:FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
Method for increasing activity in human stem cell
Provided are a method for preparing a highly active human mesenchymal stem cell, which includes forming a spherical cell aggregate by cultivating human mesenchymal stem cells against gravity; a highly active stem cell prepared thereby; a cell therapeutic agent including the stem cell aggregate; and a method for forming a spherical cell aggregate by cultivating human mesenchymal stem cells, wherein the amount of E-cadherin in the mesenchymal stem cell is increased during the cultivation.
Owner:SEOUL NAT UNIV HOSPITAL
Method for increasing activity in human stem cell
Provided are a method for preparing a highly active human mesenchymal stem cell, which includes forming a spherical cell aggregate by cultivating human mesenchymal stem cells against gravity; a highly active stem cell prepared thereby; a cell therapeutic agent including the stem cell aggregate; and a method for forming a spherical cell aggregate by cultivating human mesenchymal stem cells, wherein the amount of E-cadherin in the mesenchymal stem cell is increased during the cultivation.
Owner:SEOUL NAT UNIV HOSPITAL
Culture method of AD293 spherical cell cluster
The invention discloses a culture method of AD293 spherical cell cluster. The culture method comprises the following steps: (1) preparing a suspension culture medium: preparing the following components: a serum-free culture medium, fetal calf serum, a serum replacement, a non-essential amino acid solution, epidermal growth factor, transforming growth factor, and methyl cellulose, and evenly mixing and dissolving the components mentioned above to obtain a suspension culture medium; (2) preparing AD293 cells: using a DMEM culture medium with 10% of fetal calf serum to revive AD293 cells in a cell culture flask, and carrying out cell passage; (3) culturing the cells: adding AD293 cells obtained in the step (2) and suspension culture medium obtained in the step (1) into a shake flask, then placing the shake flask on a shaking bed, and culturing the cells for 48 to 72 hours at a temperature of 37 DEG C to obtain AD293 spherical cell clusters. Compared with the cells cultured by spherical suspension micro carriers, the cell density of obtained AD293 cell clusters is increased by 10 times or more.
Owner:ZHAOQING DAHUANONG BIOLOGIC PHARMA +1
Spherical thin-film solar cell, preparation method thereof and spatial arrangement assembly based on cell
InactiveCN101866968BFinal product manufacturePhotovoltaic energy generationTrappingTransparent conducting film
The invention relates a spherical thin-film solar cell structure, a preparation method thereof and a spatial arrangement mode of assembly composing the cell unit, belonging to the manufacturing field of the solar cell. Both a bottom electrode and an upper electrode of the spherical cell use transparent conducting films. By using the spherical structure, on one hand, sunlight in all directions canbe absorbed, thereby avoiding using a sunlight tracker; and on the other hand, light rays are refracted and reflected for many times in the small spherical body to form a good light trapping structure, thereby greatly improving the light absorption efficiency. The structure of the spherical cell assembly can enable the cells to be spatially accumulated, thereby improving the space utilization ratio to obtain a larger light receiving area. In order to ensuring the uniformity of thin film deposition, a three-dimensional rotating sample platform is used. The spherical thin-film cells are arranged in the space according to a Fibonacci spiral phyllotaxis to form the solar cell assembly. Compared with the tiled arrangement of the cells, the spatial arrangement mode can manyfold increase the light-receiving area of the cells under the condition of equal occupying area.
Owner:CHANGZHOU UNIV
W/o emulsion, foam, and functional foam
InactiveCN103068923BGood emulsificationExcellent static storage stabilityThin material handlingOil phasePore diameter
Provided is a W / O emulsion, including a continuous oil phase component and an aqueous phase component immiscible with the continuous oil phase component, in which: the continuous oil phase component includes a hydrophilic polyurethane-based polymer and an ethylenically unsaturated monomer; the hydrophilic polyurethane-based polymer includes a polyoxyethylene polyoxypropylene unit derived from polyoxyethylene polyoxypropylene glycol; and the polyoxyethylene polyoxypropylene unit includes 5 wt% to 25 wt% of polyoxyethylene. Also provided is a foam, including a hydrophilic polyurethane-based polymer, in which: the foam has an open-cell structure in which through-holes are present between adjacent spherical cells; the spherical cells each have an average pore diameter of less than 20 µm; the through-holes each have an average pore diameter of 5 µm or less; and the foam has surface openings each having an average pore diameter of 20 µm or less in a surface thereof.
Owner:NITTO DENKO CORP
Fixing-unit roller, fixing unit, and image forming apparatus including an elastic layer
A fixing-unit roller includes: a core bar; and an elastic layer formed on an outer peripheral surface of the core bar. The elastic layer is formed from a porous material that contains a plurality of cells. Cells in a cross section obtained by cutting across the porous material are 0.1 μm or greater and 50 μm or less in size. A ratio of an area occupied by composite cells, which are made of partially-overlapping spherical cells, in a 200-μm square in the cross section to an area of the square is 60% or greater and 70% or less.
Owner:RICOH KK
Preparation method of double-layer cell ball
InactiveCN102250824AReflect living environmentReflect aggressivenessTissue cultureSingle cell suspensionIn vivo
The invention relates to a preparation method of a double-layer cell ball. The invention aims at providing a preparation method of the double-layer cell ball, comprehensively and truly reflecting the survival environment of in-vivo cells, and visually reproducing the interaction between two kinds of cells under certain conditions. The technical schemes for achieving the aims are as follows: the preparation method of the double-layer cell ball comprises the steps of: a. separately culturing two different kinds of cells; b. preparing a single cell suspension from one kind of the cells; c. controlling the single cell concentration in a bottle at 1*10<5>-1*10<6> cells / ml; d. standing the single cell suspension in a 37 DEG C incubator containing 5% of CO2 for 1-4 hours; e. carrying out rotation culture on the single cell suspension for rolling the single cell suspension into balls, and changing the single cell suspension once every 10-14 hours; and f. preparing a single cell suspension of the other kind of cells, and adding the single cell suspension to the culture liquid containing the cell balls obtained in step e, continuing rotation culture so that the two kinds of cells in a tube form a spherical cell mass with a clear boundary and a double-layer structure, and changing the suspension once every 10-14 hours. The preparation method of the double-layer cell ball provided by the invention is mainly used for three-dimensional culture of cells.
Owner:ZHEJIANG UNIV
cell robot
Owner:BEIJING KEYI TECH
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