Microcavity impedance sensor for real-time monitoring of activity and proliferation ability of 3D cells and preparation method

Abstract

The invention discloses a 3D microcavity impedance sensor for real-time monitoring of activity and proliferation ability of cells and preparation method. The 3D microcavity impedance sensor is firstlymanufactured by adopting a micro-nano processing technology; 3D spherical cell culture is conducted on tumor cells; 3D spherical cells are inoculated into a trapezoidal microgroove structure in the microcavity impedance sensor, are attached to counter electrodes on the side wall of the microgroove and cause the reduction of the transfer efficiency of electrons on the electrode surfaces, so that the impedance values of the electrodes rise and are increased with diameter increase of proliferation spheres of the 3D spherical cell, and after an anti-tumor drug acts on the 3D spherical cells to cause apoptosis, the impedance values of the electrodes are decreased, and the activity and proliferation ability of the 3D spherical cells are monitored by calculating the change rate of the impedancevalues of the 3D spherical cells. The constructed microcavity impedance sensor can monitor the activity and proliferation ability of 3D spherical cells with high throughput in real time for a long time.

Description

technical field [0001] The invention relates to cell activity detection technology, in particular to a microcavity impedance sensor and a preparation method for real-time monitoring of 3D cell activity and proliferation ability. Background technique [0002] The preclinical efficacy and toxicity evaluation of candidate drugs is of great significance to drug discovery and development. Drug screening techniques often use in vitro cultured cells for primary screening of sensitive compounds. Traditional in vitro cultured cells are two-dimensional, and have problems such as contact inhibition and loss of heterogeneity. They are too different from three-dimensional cells grown in vivo, and cannot accurately reflect the situation of cells in vivo. Some drug screening results based on 2D cell experiments often show excellent curative effects, but there is no corresponding curative effect in subsequent animal experiments and clinical trials. These 2D cell-based drug screening techn...

AI Technical Summary

Problems solved by technology

At present, the activity of three-dimensional cells is mainly verified by live / dead fluorescent staining, which requires materials and equipment such as fluorescent dyes and confocal microscopes, which has problems such as low efficiency, high cost, and cumbersome operation.

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