The invention discloses a rapid propagation method for the rejuvenation of the variety of green wind
ampelopsis grossedentata. The method comprises steps of (A) selecting an explant and sterilizing, namely taking the stem tip of a tender
branch of the green wind
ampelopsis grossedentata, sterilizing the surface and reversing for later use; (B) primarily cultivating the sterilized stem tip of the tender
branch of the green wind
ampelopsis grossedentata, namely inoculating the explant subjected to the surface sterilization into a
primary culture medium under an aseptic condition, and transferring into a culture medium for inducing and differentiating clustered buds; (C) enrichment cultivating, namely
cutting the clustered buds into single plants under the aseptic condition and inoculating into a
enrichment culture medium for
enrichment culture; (D) strengthening seedlings; (E) rooting, namely
cutting the clustered seedlings cultivated through strong
seedling culture into single plants, inoculating into a rooting culture medium for rooting culture; (F)
transplanting the rooting
tissue culture seedlings into a medium, cultivating for 1-2 months so as to obtain mature seedlings. The method is easy, is simple and convenient to operate, effectively prevents the inoculating
contamination rate of the explant, has the inoculating success rate being over 85%, is capable of growing regular roots and cultivating the strong seedlings, has the
high survival rate, and is applicable to commercial production of the green wind
ampelopsis grossedentata seedlings.