A method for the in vitro extraction and determination of pectinase and amylase in the salivary enzyme of Lygus green bug

A determination method and technology for chlorotic bugs, which are applied in the measurement of color/spectral characteristics, etc., can solve the problems of difficult control of conditions, influence of results, large insect population, etc., and achieve the effects of simple and convenient operation, low production cost and small space occupation.

Inactive Publication Date: 2017-08-11
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is relatively cumbersome to operate, requires a large amount of insects, and has strict requirements on environmental conditions. The conditions are not easy to control, and improper operation will have a great impact on the results.

Method used

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  • A method for the in vitro extraction and determination of pectinase and amylase in the salivary enzyme of Lygus green bug
  • A method for the in vitro extraction and determination of pectinase and amylase in the salivary enzyme of Lygus green bug
  • A method for the in vitro extraction and determination of pectinase and amylase in the salivary enzyme of Lygus green bug

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Embodiment Construction

[0030] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.

[0031] see figure 1 Shown, it is the structural representation of the saliva extraction device of the present invention, and described device comprises Paraflim sealing film 1, top opening 2, worm chamber 3, bottom end opening 4, gauze 5, rubber band 6 and liquid nutrient medium 7, in In the present invention, the film box includes openings at both ends, the top opening 2 is covered with two layers of Paraflim film 1, and the liquid nutrient medium is between the two layers of films; Gauze 5 covers, and is fixed with rubber band 6.

[0032] The liquid nutrient medium is 20% sucrose, 100mmol / L serine, 100mmol / L methionine, 100mmol / L aspartic acid. The preparation method is to accurately weigh 20 g of sucrose, 1.05 g of serine, 1.49 g of methionine, and 1.33 g of aspartic acid, and dilute to 100 mL with di...

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Abstract

The invention relates to an in vitro extraction and measurement method for pectinase and amylase in Lygus saliva, which uses Paraflim membrane to extract the salivary enzyme of Lygus saliva, and measures the pectinase and starch in Lygus saliva Enzyme activity, Paraflim membrane method for extracting Lygus saliva Enzyme uses Paraflim membrane, film box, gauze, rubber band, liquid nutrient medium between two layers of Paraflim membranes, and uses the principle of enzyme and substrate reaction to determine the saliva of Lygus saliva Pectinase, amylase activity. The method has easy-to-obtain materials, simple and convenient operation, low production cost, and small space occupation, and can realize large-scale and batch extraction of the salivary enzyme of Lygus spp.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a method for extracting and measuring pectinase and amylase in vitro from salivary enzymes of Lygus lucidum. Background technique [0002] The green Lygus is an intermittent pest in cotton fields. It mainly uses piercing-sucking mouthparts to damage the tender tissues of plants, forming holes and causing "leaf-breaking madness". With the large-scale promotion and planting of transgenic insect-resistant cotton and the reduction of the amount of pesticides used in cotton fields, the "non-target pest" of transgenic cotton fields-the green Lygus outbreaks, and gradually continued to migrate from cotton fields to other hosts, threatening grapes, The growth of fruit trees such as apple trees, peach trees, cherries, and pear trees has become a new pest of various plants. [0003] The green Lygus is different from sap feeders such as aphids and whiteflies. It mainly feeds on the cytoplas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 周洪旭谭秀梅许秀苹王霞
Owner QINGDAO AGRI UNIV
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