The invention discloses a high-
throughput extraction method for sugarcane leaf
genome by using a
ball mill, and relates to technology for separating
genomic DNA of leaves of sugarcane by using the
ball mill aiming at the particularity of the sugarcane. Based on the characteristic that the
ball mill changes a
magnetic field to
grind a plurality of sugarcane leaf samples by
metal balls at one time, parameters such as
diameter of steel balls,
liquid nitrogen pre-
processing time, using amount of leaves,
grinding time and the like are optimized, and a method applicable to
mass extraction of the sugarcane leaf
genome is established. The method mainly comprises the steps of sampling, pre-freezing,
grinding, chemical extraction and the like. The method overcomes the disadvantages of time-consuming and labor-consuming sample
grinding, complex operation, high using amount of leaves, high cross
contamination rate, low efficiency and the like existing in the conventional preparation of the sugarcane
genomic DNA, has the advantages of wide application range, simplicity, convenience, high speed, low
contamination rate,
small sample number and the like and is particularly suitable for rapidly preparing the sugarcane leaf
genome in batches.