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Method for extracting and measuring protease and cellulase from digestive enzymes in saliva of green plant bug in vitro

A technology of cellulase and determination method, which is applied in the direction of color/spectral characteristic measurement, etc. It can solve problems such as difficult control of conditions, influence of results, large number of insects, etc., and achieve the effect of simple and convenient operation, low production cost and small space occupation

Inactive Publication Date: 2014-06-18
QINGDAO AGRI UNIV
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Problems solved by technology

This method is relatively cumbersome to operate, requires a large amount of insects, and has strict requirements on environmental conditions. The conditions are not easy to control, and improper operation will have a great impact on the results.

Method used

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  • Method for extracting and measuring protease and cellulase from digestive enzymes in saliva of green plant bug in vitro
  • Method for extracting and measuring protease and cellulase from digestive enzymes in saliva of green plant bug in vitro
  • Method for extracting and measuring protease and cellulase from digestive enzymes in saliva of green plant bug in vitro

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Embodiment Construction

[0029] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.

[0030] see figure 1 Shown, it is the structural representation of the saliva extraction device of the present invention, and described device comprises Paraflim sealing film 1, top opening 2, worm chamber 3, bottom end opening 4, gauze 5, rubber band 6 and liquid nutrient medium 7, in In the present invention, the film box includes openings at both ends, the top opening 2 is covered with two layers of Paraflim film 1, and the liquid nutrient medium is between the two layers of films; Gauze 5 covers, and is fixed with rubber band 6.

[0031] The liquid nutrient medium is 20% sucrose, 100mmol / L serine, 100mmol / L methionine, 100mmol / L aspartic acid. The preparation method is to accurately weigh 20 g of sucrose, 1.05 g of serine, 1.49 g of methionine, and 1.33 g of aspartic acid, and dilute to 100 mL with di...

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Abstract

The invention relates to a method for extracting and measuring protease and cellulase from digestive enzymes in saliva of green plant bug in vitro. The method comprises the following steps: with Parafilms, extracting digestive enzymes in saliva of green plant bug by a film clip nutrition method, and measuring the activities of protease and cellulase in the green plant bug, wherein the digestive enzymes in saliva of the green plant bug are extracted through a Parafilm method and by the Parafilms, a film canister, gauze, a rubber band, and a liquid nutrient medium between the two Parafilms, and the activities of the protease and cellulase in saliva of the green plant bug are measured according to the principle of reaction of enzymes and a substrate. The method is simple and convenient in operation, low in manufacturing cost and small in occupied space, the materials are readily available, the extraction of digestive enzymes from saliva of the green plant bug can be realized in a large scale and in batches, and the green plant bug can be continuously recycled.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a method for extracting and measuring protease and cellulase in vitro from salivary enzymes of Lygus lucidum. Background technique [0002] The green Lygus is an intermittent pest in cotton fields. It mainly uses piercing-sucking mouthparts to damage the tender tissues of plants, forming holes and causing "leaf-breaking madness". With the large-scale promotion and planting of transgenic insect-resistant cotton and the reduction of the amount of pesticides used in cotton fields, the "non-target pest" of transgenic cotton fields-the green Lygus outbreaks, and gradually continued to migrate from cotton fields to other hosts, threatening grapes, The growth of fruit trees such as apple trees, peach trees, cherries, and pear trees has become a new pest of various plants. [0003] The green Lygus is different from sap feeders such as aphids and whiteflies. It mainly feeds on the cytopla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 周洪旭谭秀梅许秀苹王霞
Owner QINGDAO AGRI UNIV
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