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A mutant strain of Nannochloropsis and its application

A technology of Nannochloropsis and mutant strains, which is applied in the field of microorganisms, can solve the problems of decreased algal cell activity, algal cell death, and damage to algal strains, and achieves strong insecticide and high temperature tolerance, and strong environmental adaptability , the effect of high fat content

Active Publication Date: 2017-08-25
ENN SCI & TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, in the large-scale outdoor cultivation of microalgae, it is easy to breed protozoa, bacteria and other pollution, which seriously pollutes the growth of algal strains.
Although the use of pesticides can effectively inhibit pollution, it will damage the algal strains, resulting in a decrease in the activity of algal cells and a reduction in production. When the concentration of pesticides is too high, the algal cells will die

Method used

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  • A mutant strain of Nannochloropsis and its application
  • A mutant strain of Nannochloropsis and its application
  • A mutant strain of Nannochloropsis and its application

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Experimental program
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Effect test

specific Embodiment 1

[0034] Screening and acquisition of specific embodiment 1ENN11-5 algal strains

[0035] The sterile algae liquid of Nannochloropsis sp. was subjected to EMS mutagenesis treatment, and sodium hypochlorite insecticide and hydroxyproline were added for repeated pressure treatment, primary screening and secondary screening, and finally a mutant strain ENN11-5 with excellent performance was obtained. The mutant algae strain is preserved in the General Microorganism Center of the China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.9321.

specific Embodiment 2

[0036] Specific embodiment 2ENN11-5 conventional culture evaluation

[0037] 1) 3cm column reactor evaluation: ENN11-5 green swimming cells in the logarithmic growth phase were inoculated in the prepared seawater medium to make the cell density reach OD 750 between 0.8-1.2, with wild-type ENN11 as the control. During the cultivation process, the light intensity is controlled at 50-500umol / m 2 .s, L:D=16:8 light-dark cycle culture. During the culture period, the pH value of the culture medium is adjusted between 7-9 by feeding a mixed gas of 1.5-2% carbon dioxide and air into the culture solution. The reactor used for the cultivation was a column reactor with an inner diameter of 30 mm and a length of 600 mm. After culturing for 8 days, the growth curve of the algae strain was measured.

[0038] Such as figure 1 As shown, the growth of ENN11-5 cultured under conventional conditions was significantly better than that of the wild strain ENN11, and the growth rate was 31.6%...

specific Embodiment 3

[0041] Specific embodiment 3ENN11-5 insecticide pressure culture evaluation

[0042] Inoculate the ENN11-5 green swimming cells in the logarithmic growth phase in the prepared seawater medium to make the cell density reach OD 750 between 0.8-1.2, with wild-type ENN11 as the control. During the cultivation process, the light intensity is controlled at 50-500umol / m 2 .s, L:D=16:8 light-dark cycle culture. During the culture period, the pH value of the culture medium is adjusted between 7-9 by feeding a mixed gas of 1.5-2% carbon dioxide and air into the culture solution. The reactor used for the cultivation was a column reactor with an inner diameter of 30 mm and a length of 600 mm. Add 25ppm available chlorine pressure at the beginning of inoculation, keep away from light for 8 hours, then culture under light, cultivate for 8 days, and measure the growth curve of the algae strain.

[0043] Such as image 3 As shown, after the addition of available chlorine pressure, the ...

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Abstract

The invention relates to a mutant strain of Nannochloropsis sp. and its application. The taxonomic name of the mutant strain of Nannochloropsis sp. is Nannochloropsis sp. The Nannochloropsis mutant strain of the invention has the advantages of high temperature and insecticide tolerance, high oil content, stable performance and strong environmental adaptability.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a mutant strain of Nannochloropsis ENN11-5 and its application. Background technique [0002] In recent years, global oil resources are becoming increasingly depleted, energy shortages are becoming more and more serious, and environmental pollution and ecological damage are becoming more and more serious. The development of green and clean biofuels to replace traditional fossil fuels has become an international research hotspot. Among the many raw materials for biofuels, microalgae have the characteristics of high photosynthetic efficiency, short growth cycle, high oil content, strong environmental adaptability, and easy genetic improvement, and are considered to be the most potential biomass resources to replace petroleum. Microalgae biomass can not only produce biodiesel, high-quality aviation gasoline and other biofuels through biosmelting, but also develop products such as platf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/64A23L33/00A01K97/04A23K10/16C12R1/89
CPCA01K97/045C12N1/12C12N1/125C12R2001/89
Inventor 李青吴洪罗少敬陈传红崔春莉
Owner ENN SCI & TECH DEV
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