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High-stability DHA algal oil preparing method

A high stability, algae oil technology, applied in the field of health care products, can solve the problems of harmfulness to human body, affect the sensory quality, nutritional value and oxidation stability of refined DHA algae oil, DHA loss, etc., and achieve the effect of increasing storage time

Inactive Publication Date: 2016-07-20
CHANGZHOU DAAO NEW MATERIAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention: at present, in order to improve the DHA anti-oxidation ability, artificially synthesized antioxidants are added, which is harmful to the human body. At the same time, the odorous substances and other small molecular impurities cannot be effectively removed in the deodorization process, thereby affecting the refined DHA algae oil. sensory quality, nutritional value and oxidation stability, and the problem that DHA in the algae oil will be lost after deodorization, the present invention first activates the chlorococcus through phytohormones to promote the growth of the chlorococcus and the increase of oil in the body, and then It is fermented with natural plants to deodorize and increase antioxidant properties under the action of natural plants, then destroy the cells of chlorococcus balls through the rich bacterial enzymes in the biogas slurry, and then make the DHA algae oil completely by high-pressure blasting released, and finally decolorized by ethanol freezing and bentonite to prepare high-stability DHA algae oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] In terms of percentage by weight, take 50 parts of sucrose, 20 parts of 10% ammonium chloride solution by mass fraction, 30 parts of peptone, 28 parts of dry corn steep liquor, 1.2 parts of indole-3-acetic acid, 8 parts of agar and 4 parts of Mass fraction is 1.3% xanthoaldehyde solution, stir evenly, use mass fraction to be 10% sodium hydroxide solution to adjust pH to 6.5, then move it to carry out sterilization under the ultraviolet disinfection lamp, get activated culture medium; Press inoculation amount of 20%, inoculated Chlorococcus in the above-mentioned activated medium, then moved the medium to a constant temperature incubator, set the temperature at 28°C, and cultivated it for 3 days. According to the mass ratio of 7:1:2, take Put the cultivated green algae, celandine and thyme in an oven, dry them at 60°C for 7 hours, put them into a pulverizer, and pass them through a 200-mesh sieve; put the sieved particles into a fermenter , the addition amount is 30% of ...

example 2

[0021] By weight percentage, get 60 parts of sucrose, 20 parts of ammonium chloride solution with a mass fraction of 10%, 30 parts of peptone, 28 parts of dry corn steep liquor, 1.2 parts of indole-3-acetic acid, 8 parts of agar and 4 parts of Mass fraction is 1.3% xanthoaldehyde solution, stir evenly, use mass fraction to be 10% sodium hydroxide solution to adjust pH to 8.0, then move it to sterilize and sterilize under ultraviolet disinfection lamp, get activated culture medium; press inoculation amount of 25%, inoculated Chlorococcus in the above-mentioned activated medium, then moved the medium to a constant temperature incubator, set the temperature at 32°C, and cultivated it for 5 days. According to the mass ratio of 7:1:2, take Put the cultured green algae, celandine and thyme into an oven, dry at 60°C for 9 hours, put them into a pulverizer, and pass through a 200-mesh sieve; put the sieved particles into a fermenter , the amount added is 35% of the volume of the ferme...

example 3

[0024] By weight percentage, take 50 parts of sucrose, 25 parts of ammonium chloride solution with a mass fraction of 10%, 32 parts of peptone, 32 parts of dry corn steep liquor, 1.5 parts of indole-3-acetic acid, 12 parts of agar and 6 parts of Mass fraction is 1.3% xanthoaldehyde solution, stir evenly, use mass fraction to be 10% sodium hydroxide solution to adjust pH to 6.5, then move it to carry out sterilization under the ultraviolet disinfection lamp, get activated culture medium; Press inoculation amount of 20%, inoculated Chlorococcus in the above-mentioned activated medium, then moved the medium to a constant temperature incubator, set the temperature at 28°C, and cultivated it for 3 days. According to the mass ratio of 7:1:2, take Put the cultivated green algae, celandine and thyme in an oven, dry them at 60°C for 7 hours, put them into a pulverizer, and pass them through a 200-mesh sieve; put the sieved particles into a fermenter , the addition amount is 30% of the ...

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Abstract

The invention relates to a high-stability DHA algal oil preparing method and belongs to the technical field of health care products. The method aims to solve the problems of the prior art that to improve the antioxidant ability of DHA, artificially synthesized antioxidant harmful to the health of people is added, matter with odor and other micromolecule impurities can not be effectively removed during deodorization, and therefore the sensor quality, nutritive value and oxidization stability of refined DHA algal oil are affected and DHA in algal oil is lost after deodorization. According to the method, chlorococcum is activated with plant hormones so that growth of chlorococcum can be promoted and in vivo grease can be increased, then chlorococcum is mixed with natural plants for mixed fermentation, deodorization is conducted under the action of the natural plants, antioxidant ability is improved at the same time, then chlorococcum spherical cells are damaged through bacterium enzyme rich in biogas slurry, DHA algal oil is completely released through high-pressure blasting, and finally ethyl alcohol refrigeration and bentonite decoloration are conducted to obtain the high-stability DHA algal oil.

Description

technical field [0001] The invention relates to a preparation method of high-stability DHA algae oil, which belongs to the technical field of health care products. Background technique [0002] Docosahexaenoic acid (DHA) is an important ω-3 long-chain polyunsaturated fatty acid. Modern medicine has proved that DHA can promote the visual and intellectual development of infants and young children, and also has a positive preventive effect on cardiovascular system diseases, cancer, and inflammation. The production of DHA has a variety of sources, among which the production of DHA algae oil by microbial fermentation has the advantages of easy large-scale cultivation, high polyunsaturated fatty acid content, easy separation and purification, and good oxidation stability. [0003] DHA has functional properties such as strengthening the brain and improving eyesight, reducing inflammation and inhibiting allergic reactions, preventing cardiovascular and cerebrovascular diseases and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6427
Inventor 潘艳丽宋奇
Owner CHANGZHOU DAAO NEW MATERIAL TECH CO LTD
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