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32 results about "Tetraselmis" patented technology

Tetraselmis is a genus of phytoplankton. Tetraselmis is a green algal genus within the order Chlorodendrales, and they are characterized by their intense green colored chloroplast, their flagellated cell bodies, the presence of a pyrenoid within the chloroplast, and a scale-produced thecal-wall. Species within this genus are found in both marine and freshwater ecosystems across the globe; their habitat range is mainly limited by water depth due to their photosynthetic nature. Thus, they live in diverse water-environments if enough nutrients and light is available for net photosynthetic activity. Tetraselmis species have proven to be useful for both research and industry. Tetraselmis species have been studied for understanding plankton growth rates, and recently a colonial colony species is being used to gain an understanding of multicellularity evolution. Additionally, many species are currently being examined for their use as biofuels due to their high lipid content.

Method for detecting oil content of microalgae

The invention relates to lipid producing microalgae, in particular relates to a method for detecting the oil content of microalgae, comprising the following steps of: 1) taking microalgae-cultivated algae liquid, and computing the quantity concentration C of microalgae cells; 2) observing the lipid of the microalgae: adopting a BODIPY (boron-dipyrromethene) 505 / 515 staining fluorescent recording method; staining for 1-2min by adopting dye use liquid with concentration of 1-2mM, and observing and recording accumulation change conditions of single-celled lipid in a photographing way by using excitation light with wave length of 488nm under a fluorescence microscope; recording the number (n) of single-celled oil drop grains according to a photograph, detecting the diameter (d) of each oil drop, and computing the volume of each oil drop according to a ball volume formula, wherein the content of each cell lipid is the sum of the volume of all the oil drops; and computing the lipid content of the algae liquid in unit volume according to the quantity concentration C of the microalgae and the lipid content of a single cell: Vt=CV, wherein C is the concentration of tetraselmis cells, V is the lipid volume of the single cell, and Vt is the lipid content in the unit volume.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Rapana venosa ecological breeding method

The invention relates to the field of Rapana venosa artificial breeding, in particular to a Rapana venosa ecological breeding method. The method comprises: transferring egg bags into a plastic basket or net cage (10-20mesh) for suspended hatch in a nursery pond and charging air continuously, wherein the density of the egg bags is 5,000 to 8,000 / m<3>; breeding larvae at a density of 1 / ml with bait made of chlamydomonas (diatom and tetraselmis are preferred) in an amount of 1.0 to 12.0*10<4> / ml every day at a water temperature of 22 to 26 DEG C; continuously charging air, changing 1 / 2 of water every day; and when the larvae grow three whorls to a size of 700 to 800 micrometers, placing the larvae in to 60-mesh net bags, cages or tanks together with double-shell spats (animal baits for transformed Rapana venosa) for sea breeding.
Owner:SHANDONG UNIV AT WEIHAI

Cyclinasinensis purification reproduction and culture method

The invention discloses a cyclinasinensis purification reproduction and culture method. The method includes the processes of temporary culture of parent clams, spawning of the parent clams, trochophore hatching, D-larvae and post-larvae culture and juvenile clam culture. Microbial preparations are thrown in during the process of temporary culture of the parent clams, a chitosan decontamination agent is thrown in during the process of spawning of the parent clams, the microbial preparations are thrown in during the course of trochophore hatching, microalgae preparations are thrown in during the course of D-larvae and post-larvae culture, and juvenile clams are fed with tetraselmis in the period of juvenile clam culture till grown-up cyclinasinensis is harvested. Purification substances are thrown in the whole period from temporary culture of the parent clams to larvae culture and juvenile clam culture for purifying meat, pollution of contaminants is avoided from the source, healthiness, greenness and non-pollution in the culture process are guaranteed, the contaminants such as heavy metal, petroleum and nitrofuran derivatives in bodies of the grown-up cyclinasinensis are reduced significantly, and good effect on removal of escherichia coli, sphingomonaspaucimobilis, vibrio, aeromonassalmonicida and the like is achieved, and the grown-up cyclinasinensis completely conforming to food security can be cultured.
Owner:OCEAN UNIV OF CHINA +1

Ecological Japanese sea horse breeding method

InactiveCN103960185ASuitable for artificial breedingClimate change adaptationPisciculture and aquariaShrimpJuvenile fish
The invention discloses an ecological Japanese sea horse breeding method. The ecological Japanese sea horse breeding method comprises the steps of building an ecological sea horse breeding pond one week before breeding, cultivating microalgae in the built ecological breeding pond, and measuring and controlling water quality to reach newborn sea horse breeding indexes before newborn sea horses are bred. When the newborn sea horses is younger than 15 days, the released density is 1000-1200 tail / m<3>, and baits are composed of rotifer, copepods larvae, chlorella, photosynthetic bacteria, tetraselmis and fairy shrimps which are just incubated. During 15-30 days, the released density is 600-1000 tail / m<3>, baits are composed of fairy shrimps, copepods larvae and shrimp seeds, and the baits are fed into the breeding pond four times per day. A sea horse juvenile fish breeding stage comprises the step of enabling the water quality to reach juvenile fish breeding indexes before breeding. During 30-40 days, the released density is 600-800 tail / m<3>, and the baits are fed into the breeding pond three times per day. A sea horse adult fish breeding stage comprises the step of enabling the water quality to reach sea horse adult fish breeding indexes. The released density of sea horse adult fish is 200-400 tail / m<3>, baits are fresh and alive shrimps, and the baits are fed into the breeding pond three times per day. The ecological Japanese sea horse breeding method has the advantages of reducing production cost.
Owner:惠安港德海洋生物科技有限公司

Method for manually breeding Pinna (Atriina) pectinata Linnaeus

The invention aims at providing a method for breeding Pinna (Atriina) pectinata Linnaeus. The method comprises the following steps of: selecting the Pinna (Atriina) pectinata Linnaeus as a parent shellfish, accelerating the ripening of the Pinna (Atriina) pectinata Linnaeus so the parent shellfish gradually matures; drying the parent shellfish in the shadow, radiating the parent shellfish for 3 hours with a 30W ultraviolet lamp to ensure that the mature parent shellfish leaves sperms and ova which are subjected to fertilization in water and develop into a D-shaped veliger larva in 25 hours after fertilization; collecting the D-shaped veliger larva by a 300-mesh trawl when the larva is developed into the D-shaped veliger larva, breeding the D-shaped veliger larva in a breeding pond; throwing larva collection bags to collect the larva when the larva has legs; rinsing juvenile mollusk, and overturning the larva collection bags, strewing the juvenile mollusk to the other pond spread with sands, wherein the food organism attached by the juvenile mollusk is tetraselmis and pyramimonas sp.
Owner:SHANDONG HAIYIBAO AQUATIC PROD

Process for isolating a natural red pigment from a species of microalgae, Tetraselmis sp. MACC/P66

A species of marine microalgae (Tetraselmis sp. MACC / P66) was found to secrete an unknown red pigment to the culture medium when itself was still keeping healthy.The unknown red pigment was collected and purified from the culture medium by column chromatography using cation-exchange resins. A process for concentrating and isolating the red water soluble pigment from the culture medium of Tetraselmis sp. MACC / P66 was described in the present invention.
Owner:ZHANG YUSHI

Seedlings-cultivating method of atrina pectinata

The invention provides a seedlings-cultivating method of the atrina pectinata, which comprises the steps of: selecting the atrina pectinata as a parent scallop, so that the parent scallop is gradually matured by means of fertilizing and cultivating; drying the parent scallop in the shade, and irradiating the dried parent scallop by a 30W ultraviolet lamp for 3 hours, so that the matured scallop discharges the sperm and the ovum, and the sperm and the ovum are voluntarily fertilized in the water; after the fertilization, cultivating to form into the D-shaped veliger larva after 25 hours; when the larva is cultivated to be the D-shaped veliger larva, collecting the D-shaped veliger larva into a seedlings-cultivating pond to cultivate by a 300 screen mesh dragnet; when the larva cultivates the feet, acquiring the seedlings by putting a seedlings acquiring bag; and overturning the seedlings acquiring bag after the juvenile scallop is washed, and splashing the juvenile scallop into another pond paved with the sand, wherein the bait organism is the tetraselmis and the pyramimonas sp after the juvenile scallop is attached.
Owner:SHANDONG HAIYIBAO AQUATIC PROD

Artificial breeding method of Spisula sachalinensis (Schrenck)

InactiveCN101990853AEstablishment of Artificial Seed Breeding MethodClimate change adaptationPisciculture and aquariaRipeningIsochrysis zhanjiangensis
The invention discloses an artificial breeding method of Spisula sachalinensis (Schrenck), which is characterized by comprising the following steps: fishing and catching individuals as parents from a natural environment when the water temperature in a natural sea area is below 12 DEG C in spring; placing the parents in an indoor water body for warming and ripening, feeding the parents with spirulina powder, egg yolk, starch and unicellular algae baits, and breeding at a constant temperature; stimulating the parents to discharge ova and sperms by a chemical method, and incubating oosperms into D-shaped larvas; transferring the D-shaped larvas into a pond for breeding by a silk net, wherein the larva breeding density is 0.5-1 larva / ml, and the D-shaped larvas are fed with Isochrysis zhanjiangensis at the early stage, and are fed with the Isochrysis zhanjiangensis and Tetraselmis at the later stage; collecting and transferring the larvas to another pond for breeding when the shell of the larva grows to 280-300 mu m and feet grows out, and enabling the larvas to finish settlement and metamorphosis at the bottom of the pond by adopting an adherence-free collection technology; and transferring juvenile mollusks to the outdoor for intermediate breeding when the shell of the mollusk grows to 1.5-2mm. By the artificial breeding method of the invention, artificial seedlings of Spisula sachalinensis (Schrenck) can be bred in a large scale.
Owner:OCEAN UNIV OF CHINA

Thais bronni breeding method

The invention provides a Thais bronni breeding method. The Thais bronni breeding method comprises the following steps of: collecting and incubating fertilized eggs; cultivating larvae; and cultivating juvenile mollusks, wherein the fed baits in the pelagic period of larva cultivation comprise chrysophyceae, chlorella, tetraselmis and chaetoceros in a ratio of 10:1:1:1; during metamorphosis period before juvenile mollusk period, the varieties and the amounts of the fed baits, including shrimp slices and razor clam meat, are increased along with time; and the prawn slices and the razor clam meat with a certain ratio are maintained during cultivation of the juvenile mollusks. According to the thais bronni breeding method, different bait constitutions and feeding amounts are provided for successful growth of the thais bronni in each development stage of the thais bronni. Compared with the prior art without artificial breeding, the method establishes the basis of artificial breeding of the thais bronni, so that artificial culture of the thais bronni can meet the requirements of the market.
Owner:SHENGSI COUNTY DONGHAI MUSSELS TECH INNOVATION SERVICE

Soil microalgae of mining area, separation and purification method for soil microalgae of mining area and application of soil microalgae of mining area

The invention provides soil microalgae of a mining area, a separation and purification method for the soil microalgae of the mining area and application of the soil microalgae of the mining area. Thesoil microalgae of the mining area are separated and purified from biological crusts of surfaces of tailings and comprise one or more selected from Chlorella, Cylindrotheca, Diatom, Nitzschia, Schizochytrium, Dunaliella, Scenedesmus, Nannochloropsis oculata, Chlamydomonas, Tetraselmis and Eudovina. The soil microalgae of the mining area, provided by the invention, are separated and purified from the biological crusts of the surfaces of the tailings; the obtained microalgae are relatively extensive in variety; and the obtained microalgae have relatively good adsorption and absorption capabilityto contaminants such as heavy metals and phosphorus in contaminated soil and water and then can effectively treat the contaminants such as the heavy metals and phosphorus in the contaminated soil andwater, so that the treatment cost of the contaminants such as the heavy metals and phosphorus in the contaminated soil and water is reduced greatly.
Owner:WUHAN UNIV OF TECH

Microbial water purifying agent for snakehead culture water and preparation method thereof

The invention discloses a microbial water purifying agent for snakehead culture water and a preparation method thereof. The preparation method comprises the following steps: adding 20-40 parts by weight of surface-modified C8 and C18 alkyl-chain magnetic silicon balls into 200-400 parts by weight of water; adding 80-100 parts by weight of microbial reagent, including 60-80 parts of Synechococcus, 60-80 parts of Oscillatoria, 10-30 parts of Nitrosococcus, 10-30 parts of Nitrosolobus, 10-20 parts of iron bacteria, 10-20 parts of Leptothrix, 1-5 parts of Thiothrix, 1-5 parts of Vitreoscilla, 30-70 parts of paramecium, 30-70 parts of infusorian, 30-50 parts of Tetraselmis and 30-50 parts of blue algae; and adding 50-90 parts by weight of beef extract peptone agar culture medium, and culturing. The microbial water purifying agent can be added into a snakehead culture tank to achieve the goal of purifying culture tank water.
Owner:贺兰县晶诚水产养殖有限公司

Microbial water purifying agent for mandarin fish culture water and preparation method thereof

The invention discloses a microbial water purifying agent for mandarin fish culture water and a preparation method of the agent. The preparation method comprises the following steps: adding 40-50 parts by weight of magnetic silicon spheres of which the surface is modified with C8 and C18 alkyl chains to 200-250 parts by weight of water; adding 100-150 parts by weight of microbial reagents which include 40-60 parts of synechococcus, 40-50 parts of rhodobacter sphaeroides, 30-40 parts of nitrospina, 30-50 parts of nitrococcus, 20-40 parts of sphaerotilus natans, 30-50 parts of pseudomonas aeruginosa, 3-5 parts of leucothrix, 3-5 parts of thioploca, 50-60 parts of amoeba, 30-50 parts of suctorida, 30-40 parts of dunaliella and 30-50 parts of tetraselmis; next, adding 50-80 parts by weight of beef extract peptone agar culture medium for culturing. The microbial water purifying agent is put into a mandarin fish culture pond to purify water in the culture pond.
Owner:SHANGHAI KUIYA ENVIRONMENTAL PROTECTION TECH

Purification method of cyclina sinensis finished product

The invention discloses a purification method of a cyclina sinensis finished product. The purification method comprises the following steps: washing an obtained cyclina sinensis finished product; removing sludge; putting into a temporary cultivation tank; during a cultivation period, adding a microalgae preparation into sand-filtered seawater, wherein the microalgae is a mixture of skeletonema, tetraselmis and dicrateria inornata which are mixed at a random ratio; after 5 hours, putting the cyclina sinensis finished product into another temporary cultivation tank; adding a microbial preparation into the sand-filtered seawater, wherein the microbial preparation contains agritol freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, wild mushroom entity freeze-dried powder and diatomite; and culturing for 24-48 hours, renewing the water and further culturing for 4-8 hours to obtain a purified finished product. The purification method disclosed by the invention has the advantages of effectively removing heavy metals in the body of the cyclina sinensis within short time, having a quite good removal effect on escherichia coli, sphingomonas paucimobilis, vibrio and aeromonas salmonicida, and is simple to operate, short in purification time and extremely good in purification efficiency.
Owner:BINZHOU OCEAN & FISHERIES RES INST

A kind of microbial water purifying agent for black fish culture water and preparation method thereof

ActiveCN104276665BSuitable for online processingEasy to settleBiological water/sewage treatmentSynechococcusThiothrix
The invention discloses a microbial water purifying agent for snakehead culture water and a preparation method thereof. The preparation method comprises the following steps: adding 20-40 parts by weight of surface-modified C8 and C18 alkyl-chain magnetic silicon balls into 200-400 parts by weight of water; adding 80-100 parts by weight of microbial reagent, including 60-80 parts of Synechococcus, 60-80 parts of Oscillatoria, 10-30 parts of Nitrosococcus, 10-30 parts of Nitrosolobus, 10-20 parts of iron bacteria, 10-20 parts of Leptothrix, 1-5 parts of Thiothrix, 1-5 parts of Vitreoscilla, 30-70 parts of paramecium, 30-70 parts of infusorian, 30-50 parts of Tetraselmis and 30-50 parts of blue algae; and adding 50-90 parts by weight of beef extract peptone agar culture medium, and culturing. The microbial water purifying agent can be added into a snakehead culture tank to achieve the goal of purifying culture tank water.
Owner:贺兰县晶诚水产养殖有限公司

METHOD FOR OBTAINING A BIOMASS OF A MICROALGA OF THE SPECIES Tetraselmis chuii ENRICHED IN SUPEROXIDE DISMUTASE (SOD)

The invention relates to a method for enriching a biomass of a microalga of the species Tetraselmis chuii in superoxide dismutase (SOD) by placing said microalga under abiotic stress conditions. The invention also relates to a biomass enriched in SOD as well as to an extract of the microalga and to the uses thereof as a pharmaceutical composition, as a cosmetic or in foodstuff.
Owner:FITOPLANCTON MARINO SL

Nutrition reinforcement method of improving artemia growth and increasing EPA (eicosapentaenoic acid) content

The invention discloses a nutrition reinforcement method of improving artemia growth and increasing an EPA (eicosapentaenoic acid) content. Continuous feeding of tetraselmis can effectively improve the artemia growth and increase the EPA content when an artemia grows to 3 years. The method can increase a utilization rate of algae food, improves growth quality of the artemia and provides high-quality bait for aquaculture.
Owner:NANTONG SCI & TECH VOCATIONAL COLLEGE

Screening and cultural method for initial feed bestiolina similis of ocean fancy fishes

The invention provides a screening and cultural method for an initial feed bestiolina similis of ocean fancy fishes, and relate to living food culture. The method comprises: using a 500-1000 L glass fiber reinforced plastic or polycarbonate resin water tank as a culture container, culturing indoors, ocean temperature being 22-28 DEG C, salinity being 2.5-3 %, illuminance being 700-1000 lux, photoperiod being 12L:12D, and maintaining micro inflation; feeding different microalgaes in a mixed manner every day, in inoculation, feeding isochrysis (30,000 cell / mL) and tetraselmis (3000 cell / mL) in a mixed manner every day; in a medium-term, adding to feed pavlova viridis (40,000 cell / mL); when culture density of the bestiolina similis reaches 3000 individuals / L, the number of bait is doubled than the number in the medium-term; siphoning residual feed and faeces every day, and collecting part of nauplius and copepodite as the initial feed of ocean fancy fishes, and adding necessary alga; and after 1 month of continuous culture, changing water thoroughly.
Owner:XIAMEN UNIV

Method for detecting oil content of microalgae

The invention relates to lipid producing microalgae, in particular relates to a method for detecting the oil content of microalgae, comprising the following steps of: 1) taking microalgae-cultivated algae liquid, and computing the quantity concentration C of microalgae cells; 2) observing the lipid of the microalgae: adopting a BODIPY (boron-dipyrromethene) 505 / 515 staining fluorescent recording method; staining for 1-2min by adopting dye use liquid with concentration of 1-2mM, and observing and recording accumulation change conditions of single-celled lipid in a photographing way by using excitation light with wave length of 488nm under a fluorescence microscope; recording the number (n) of single-celled oil drop grains according to a photograph, detecting the diameter (d) of each oil drop, and computing the volume of each oil drop according to a ball volume formula, wherein the content of each cell lipid is the sum of the volume of all the oil drops; and computing the lipid content of the algae liquid in unit volume according to the quantity concentration C of the microalgae and the lipid content of a single cell: Vt=CV, wherein C is the concentration of tetraselmis cells, V is the lipid volume of the single cell, and Vt is the lipid content in the unit volume.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Method for breeding high-quality pearl young shellfishes

The invention provides a method for propagating high-quality pearl young shellfishes. The method comprises the following steps of 1, spawning induction and fertilization, wherein parent shellfishes are taken out and contained in cages with male shellfishes and female shellfishes separated, the parent shellfishes are placed in treatment seawater containing ammonium hydroxide, the parent shellfishesare taken out to be dried in the shade 1 hour later, a spawning induction pond is filled with the treatment seawater, tetraselmis is put into the spawning induction pond, then the parent shellfishesare placed in the spawning induction pond, the male shellfishes and the female shellfishes are contained in different cages, atmosphere oxygenating is carried out until the water surface is boiled, the parent shellfishes are removed 1 hour later, the spawning induction pond is thoroughly washed, the parent shellfishes are transferred into a fertilization pond half an hour later, and the parent shellfishes are removed 4 hours later; 2, larva management; 3, water quality management, wherein the water is replaced once every three days, and the water replacement amount is 1 / 10 of the volume. Whenthe method is used for propagating the high-quality pearl young shellfishes, the water replacement amount is small, the water quality is stable, the death rate at the stage that the larvae are transformed into the young shellfishes is low, the yield is high, and the labor cost and the electricity cost are low.
Owner:广西精工海洋科技有限公司

Penaeus-japonicus special seedling culturing bait and preparing method thereof

The invention discloses a penaeus-japonicus special seedling culturing bait. The penaeus-japonicus special seedling culturing bait is prepared from, by weight, 10-14 parts of diatom, 10-14 parts of tetraselmis, 10-14 parts of Dunaliella, 8-12 parts of chlorella, 5-8 parts of ulva, 15-20 parts of oyster meat, 16-18 parts of eggs, 20-30 parts of water fleas, 25-30 parts of rotifera, 28-34 parts of artemia and 30-40 parts of fairy shrimps. The penaeus-japonicus special seedling culturing bait has the advantages that the survival rate of shrimp seeds can be effectively increased, the palatability is high, nutrition is rich, and the penaeus-japonicus special seedling culturing bait is suitable for large-scale production; compared with an existing product, the survival rate of shrimp seeds can be increased by 5% to 10%, and market prospects are good.
Owner:GUANGXI ZHENGWU MARINE IND

Artificial breeding method of Spisula sachalinensis (Schrenck)

InactiveCN101990853BEstablishment of Artificial Seed Breeding MethodClimate change adaptationPisciculture and aquariaRipeningIsochrysis zhanjiangensis
The invention discloses an artificial breeding method of Spisula sachalinensis (Schrenck), which is characterized by comprising the following steps: fishing and catching individuals as parents from a natural environment when the water temperature in a natural sea area is below 12 DEG C in spring; placing the parents in an indoor water body for warming and ripening, feeding the parents with spirulina powder, egg yolk, starch and unicellular algae baits, and breeding at a constant temperature; stimulating the parents to discharge ova and sperms by a chemical method, and incubating oosperms into D-shaped larvas; transferring the D-shaped larvas into a pond for breeding by a silk net, wherein the larva breeding density is 0.5-1 larva / ml, and the D-shaped larvas are fed with Isochrysis zhanjiangensis at the early stage, and are fed with the Isochrysis zhanjiangensis and Tetraselmis at the later stage; collecting and transferring the larvas to another pond for breeding when the shell of the larva grows to 280-300 mu m and feet grows out, and enabling the larvas to finish settlement and metamorphosis at the bottom of the pond by adopting an adherence-free collection technology; and transferring juvenile mollusks to the outdoor for intermediate breeding when the shell of the mollusk grows to 1.5-2mm. By the artificial breeding method of the invention, artificial seedlings of Spisula sachalinensis (Schrenck) can be bred in a large scale.
Owner:OCEAN UNIV OF CHINA

A kind of clam product purification method

The invention discloses a purification method of a cyclina sinensis finished product. The purification method comprises the following steps: washing an obtained cyclina sinensis finished product; removing sludge; putting into a temporary cultivation tank; during a cultivation period, adding a microalgae preparation into sand-filtered seawater, wherein the microalgae is a mixture of skeletonema, tetraselmis and dicrateria inornata which are mixed at a random ratio; after 5 hours, putting the cyclina sinensis finished product into another temporary cultivation tank; adding a microbial preparation into the sand-filtered seawater, wherein the microbial preparation contains agritol freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, wild mushroom entity freeze-dried powder and diatomite; and culturing for 24-48 hours, renewing the water and further culturing for 4-8 hours to obtain a purified finished product. The purification method disclosed by the invention has the advantages of effectively removing heavy metals in the body of the cyclina sinensis within short time, having a quite good removal effect on escherichia coli, sphingomonas paucimobilis, vibrio and aeromonas salmonicida, and is simple to operate, short in purification time and extremely good in purification efficiency.
Owner:BINZHOU OCEAN & FISHERIES RES INST

Microbial water purifying agent for puffer fish culture water and preparation method thereof

The invention discloses a microbial water purifying agent for puffer fish culture water and a preparation method thereof. The preparation method comprises the following steps: adding 30-40 parts by weight of surface-modified C8 and C18 alkyl-chain magnetic silicon balls into 300-400 parts by weight of water; adding 80-120 parts by weight of microbial reagent, including 60-80 parts of Synechococcus, 50-70 parts of Rhodobacter sphaeroides, 20-30 parts of Nitrosospira, 20-30 parts of Nitrosococcus, 10-30 parts of Pseudomonas stutzeri, 20-30 parts of iron bacteria, 3-6 parts of Thiothrix, 3-6 parts of Beggiatoa, 50-80 parts of Sarcomastigophora, 50-70 parts of amoebae, 30-50 parts of Tetraselmis and 30-40 parts of dunaliella tertiolectra; and adding 60-80 parts by weight of beef extract peptone agar culture medium, and culturing. The microbial water purifying agent can be added into a puffer fish culture tank to achieve the goal of purifying culture tank water.
Owner:徐州邦尼生物技术有限公司

A kind of clam purification propagation cultivation method

The invention discloses a cyclinasinensis purification reproduction and culture method. The method includes the processes of temporary culture of parent clams, spawning of the parent clams, trochophore hatching, D-larvae and post-larvae culture and juvenile clam culture. Microbial preparations are thrown in during the process of temporary culture of the parent clams, a chitosan decontamination agent is thrown in during the process of spawning of the parent clams, the microbial preparations are thrown in during the course of trochophore hatching, microalgae preparations are thrown in during the course of D-larvae and post-larvae culture, and juvenile clams are fed with tetraselmis in the period of juvenile clam culture till grown-up cyclinasinensis is harvested. Purification substances are thrown in the whole period from temporary culture of the parent clams to larvae culture and juvenile clam culture for purifying meat, pollution of contaminants is avoided from the source, healthiness, greenness and non-pollution in the culture process are guaranteed, the contaminants such as heavy metal, petroleum and nitrofuran derivatives in bodies of the grown-up cyclinasinensis are reduced significantly, and good effect on removal of escherichia coli, sphingomonaspaucimobilis, vibrio, aeromonassalmonicida and the like is achieved, and the grown-up cyclinasinensis completely conforming to food security can be cultured.
Owner:OCEAN UNIV OF CHINA +1

Screening of high-temperature-resistant and high-light-resistant Tetraselmis

The invention relates to the technical field of microalgae culture, in particular to screening of high-temperature-resistant and high-light-resistant Tetraselmis. The Tetraselmis is obtained through screening, the number is CCTCC NO: M20211216, the OD750 cultured at the high temperature of 35 DEG C can reach 3.0 or above, and the growth vigor of the Tetraselmis under the illumination condition of 200 [mu] E.m <-2 >. S <-1 > is better than that under the illumination condition of 50 [mu] E.m <-2 >. S <-1 >. Under high-temperature and high-light culture conditions, the algal strain is high in density, the dry weight of the algal strain contains 48.156% of protein (including abundant essential amino acids), 24.57% of carbohydrate, 17.109% of starch and 12.499% of grease, and polyunsaturated fatty acid accounts for 62.3% of total fatty acid. The algal strain is rich in nutrition and suitable for being used as bait for fishes and shrimps.
Owner:HAINAN UNIVERSITY

A kind of ecological cultivation method of Japanese seahorse

InactiveCN103960185BSuitable for artificial breedingClimate change adaptationPisciculture and aquariaJuvenile fishPrawn
The invention discloses an ecological Japanese sea horse breeding method. The ecological Japanese sea horse breeding method comprises the steps of building an ecological sea horse breeding pond one week before breeding, cultivating microalgae in the built ecological breeding pond, and measuring and controlling water quality to reach newborn sea horse breeding indexes before newborn sea horses are bred. When the newborn sea horses is younger than 15 days, the released density is 1000-1200 tail / m<3>, and baits are composed of rotifer, copepods larvae, chlorella, photosynthetic bacteria, tetraselmis and fairy shrimps which are just incubated. During 15-30 days, the released density is 600-1000 tail / m<3>, baits are composed of fairy shrimps, copepods larvae and shrimp seeds, and the baits are fed into the breeding pond four times per day. A sea horse juvenile fish breeding stage comprises the step of enabling the water quality to reach juvenile fish breeding indexes before breeding. During 30-40 days, the released density is 600-800 tail / m<3>, and the baits are fed into the breeding pond three times per day. A sea horse adult fish breeding stage comprises the step of enabling the water quality to reach sea horse adult fish breeding indexes. The released density of sea horse adult fish is 200-400 tail / m<3>, baits are fresh and alive shrimps, and the baits are fed into the breeding pond three times per day. The ecological Japanese sea horse breeding method has the advantages of reducing production cost.
Owner:惠安港德海洋生物科技有限公司

A kind of microbial water purifying agent for hairy crab breeding water and preparation method thereof

InactiveCN104276664BSuitable for online processingEasy to settleBiological water/sewage treatmentSynechococcusIron bacteria
The invention discloses a microbial water purifying agent for hairy crab culture water and a preparation method of the agent. The preparation method comprises the following steps: adding 20-40 parts by weight of magnetic silicon spheres of which the surface is modified with C8 and C18 alkyl chains to 200-400 parts by weight of water; adding 130-180 parts by weight of microbial reagents which include 30-60 parts of synechococcus, 30-50 parts of rhodospirillum, 10-30 parts of nitrococcus, 10-30 parts of nitrospina, 10-20 parts of thiobacillus denitrificans, 10-20 parts of raw iron bacteria, 3-5 parts of beggiatoa, 1-3 parts of vitreoscilla, 30-70 parts of phytoflagellate, 30-50 parts of zooflagellate, 20-50 parts of tetraselmis and 20-50 parts of chlorella; next, adding 60-80 parts by weight of beef extract peptone agar culture medium for culturing. The microbial water purifying agent is put into a hairy crab culture pond to purify water in the culture pond.
Owner:珠海康普生物科技有限公司

Culture medium and culture method of Tetraphyllum hypocardioid by using wastewater from winery

The invention discloses a method for cultivating Tetraselmis subcordiformis by utilizing winery wastewater. The technique can treat wine wastewater at low cost, change wastes into valuable substances, and obtain Tetraselmis subcordiformis with important bait value, thereby implementing multiple purposes. The traditional Tetraselmis subcordiformis culture solution has fewer nutrients and uses an open cement track tank for culture, and thus, has the defects of low growth speed, low yield, high investment, high tendency to pollution and low benefit. At present, a water tower made from high-density polyethylene with the volume of 3 cubic meters, which has the advantages of low cost and low tendency to pollution, can be reutilized and is easy to operate, is adopted; and wine wastewater, urea sea mud extracting solution, soil extracting solution, cattle manure leaching solution, L-cysteine, casein peptone, medical stone powder, sodium dihydrogen phosphate, S3 vitamin, human urine and other nutritional ingredients are added into the culture medium, and the organic fertilizer and inorganic fertilizer are mixed for use, so that the nutrition is more comprehensive and balanced, thereby greatly enhancing the growth speed of the Tetraselmis subcordiformis and enhancing the yield by 350%.
Owner:江西康膳源油脂有限公司
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