32 results about "Tetraselmis" patented technology

The invention relates to lipid producing microalgae, in particular relates to a method for detecting the oil content of microalgae, comprising the following steps of: 1) taking microalgae-cultivated algae liquid, and computing the quantity concentration C of microalgae cells; 2) observing the lipid of the microalgae: adopting a BODIPY (boron-dipyrromethene) 505 / 515 staining fluorescent recording method; staining for 1-2min by adopting dye use liquid with concentration of 1-2mM, and observing and recording accumulation change conditions of single-celled lipid in a photographing way by using excitation light with wave length of 488nm under a fluorescence microscope; recording the number (n) of single-celled oil drop grains according to a photograph, detecting the diameter (d) of each oil drop, and computing the volume of each oil drop according to a ball volume formula, wherein the content of each cell lipid is the sum of the volume of all the oil drops; and computing the lipid content of the algae liquid in unit volume according to the quantity concentration C of the microalgae and the lipid content of a single cell: Vt=CV, wherein C is the concentration of tetraselmis cells, V is the lipid volume of the single cell, and Vt is the lipid content in the unit volume.

The invention provides a Thais bronni breeding method. The Thais bronni breeding method comprises the following steps of: collecting and incubating fertilized eggs; cultivating larvae; and cultivating juvenile mollusks, wherein the fed baits in the pelagic period of larva cultivation comprise chrysophyceae, chlorella, tetraselmis and chaetoceros in a ratio of 10:1:1:1; during metamorphosis period before juvenile mollusk period, the varieties and the amounts of the fed baits, including shrimp slices and razor clam meat, are increased along with time; and the prawn slices and the razor clam meat with a certain ratio are maintained during cultivation of the juvenile mollusks. According to the thais bronni breeding method, different bait constitutions and feeding amounts are provided for successful growth of the thais bronni in each development stage of the thais bronni. Compared with the prior art without artificial breeding, the method establishes the basis of artificial breeding of the thais bronni, so that artificial culture of the thais bronni can meet the requirements of the market.

The invention discloses a microbial water purifying agent for snakehead culture water and a preparation method thereof. The preparation method comprises the following steps: adding 20-40 parts by weight of surface-modified C8 and C18 alkyl-chain magnetic silicon balls into 200-400 parts by weight of water; adding 80-100 parts by weight of microbial reagent, including 60-80 parts of Synechococcus, 60-80 parts of Oscillatoria, 10-30 parts of Nitrosococcus, 10-30 parts of Nitrosolobus, 10-20 parts of iron bacteria, 10-20 parts of Leptothrix, 1-5 parts of Thiothrix, 1-5 parts of Vitreoscilla, 30-70 parts of paramecium, 30-70 parts of infusorian, 30-50 parts of Tetraselmis and 30-50 parts of blue algae; and adding 50-90 parts by weight of beef extract peptone agar culture medium, and culturing. The microbial water purifying agent can be added into a snakehead culture tank to achieve the goal of purifying culture tank water.

The invention relates to lipid producing microalgae, in particular relates to a method for detecting the oil content of microalgae, comprising the following steps of: 1) taking microalgae-cultivated algae liquid, and computing the quantity concentration C of microalgae cells; 2) observing the lipid of the microalgae: adopting a BODIPY (boron-dipyrromethene) 505 / 515 staining fluorescent recording method; staining for 1-2min by adopting dye use liquid with concentration of 1-2mM, and observing and recording accumulation change conditions of single-celled lipid in a photographing way by using excitation light with wave length of 488nm under a fluorescence microscope; recording the number (n) of single-celled oil drop grains according to a photograph, detecting the diameter (d) of each oil drop, and computing the volume of each oil drop according to a ball volume formula, wherein the content of each cell lipid is the sum of the volume of all the oil drops; and computing the lipid content of the algae liquid in unit volume according to the quantity concentration C of the microalgae and the lipid content of a single cell: Vt=CV, wherein C is the concentration of tetraselmis cells, V is the lipid volume of the single cell, and Vt is the lipid content in the unit volume.

The invention discloses an artificial breeding method of Spisula sachalinensis (Schrenck), which is characterized by comprising the following steps: fishing and catching individuals as parents from a natural environment when the water temperature in a natural sea area is below 12 DEG C in spring; placing the parents in an indoor water body for warming and ripening, feeding the parents with spirulina powder, egg yolk, starch and unicellular algae baits, and breeding at a constant temperature; stimulating the parents to discharge ova and sperms by a chemical method, and incubating oosperms into D-shaped larvas; transferring the D-shaped larvas into a pond for breeding by a silk net, wherein the larva breeding density is 0.5-1 larva / ml, and the D-shaped larvas are fed with Isochrysis zhanjiangensis at the early stage, and are fed with the Isochrysis zhanjiangensis and Tetraselmis at the later stage; collecting and transferring the larvas to another pond for breeding when the shell of the larva grows to 280-300 mu m and feet grows out, and enabling the larvas to finish settlement and metamorphosis at the bottom of the pond by adopting an adherence-free collection technology; and transferring juvenile mollusks to the outdoor for intermediate breeding when the shell of the mollusk grows to 1.5-2mm. By the artificial breeding method of the invention, artificial seedlings of Spisula sachalinensis (Schrenck) can be bred in a large scale.

The invention discloses a purification method of a cyclina sinensis finished product. The purification method comprises the following steps: washing an obtained cyclina sinensis finished product; removing sludge; putting into a temporary cultivation tank; during a cultivation period, adding a microalgae preparation into sand-filtered seawater, wherein the microalgae is a mixture of skeletonema, tetraselmis and dicrateria inornata which are mixed at a random ratio; after 5 hours, putting the cyclina sinensis finished product into another temporary cultivation tank; adding a microbial preparation into the sand-filtered seawater, wherein the microbial preparation contains agritol freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, wild mushroom entity freeze-dried powder and diatomite; and culturing for 24-48 hours, renewing the water and further culturing for 4-8 hours to obtain a purified finished product. The purification method disclosed by the invention has the advantages of effectively removing heavy metals in the body of the cyclina sinensis within short time, having a quite good removal effect on escherichia coli, sphingomonas paucimobilis, vibrio and aeromonas salmonicida, and is simple to operate, short in purification time and extremely good in purification efficiency.

The invention provides a Thais bronni breeding method. The Thais bronni breeding method comprises the following steps of: collecting and incubating fertilized eggs; cultivating larvae; and cultivating juvenile mollusks, wherein the fed baits in the pelagic period of larva cultivation comprise chrysophyceae, chlorella, tetraselmis and chaetoceros in a ratio of 10:1:1:1; during metamorphosis period before juvenile mollusk period, the varieties and the amounts of the fed baits, including shrimp slices and razor clam meat, are increased along with time; and the prawn slices and the razor clam meat with a certain ratio are maintained during cultivation of the juvenile mollusks. According to the thais bronni breeding method, different bait constitutions and feeding amounts are provided for successful growth of the thais bronni in each development stage of the thais bronni. Compared with the prior art without artificial breeding, the method establishes the basis of artificial breeding of the thais bronni, so that artificial culture of the thais bronni can meet the requirements of the market.

The invention discloses a nutrition reinforcement method of improving artemia growth and increasing an EPA (eicosapentaenoic acid) content. Continuous feeding of tetraselmis can effectively improve the artemia growth and increase the EPA content when an artemia grows to 3 years. The method can increase a utilization rate of algae food, improves growth quality of the artemia and provides high-quality bait for aquaculture.

The invention discloses a cyclinasinensis purification reproduction and culture method. The method includes the processes of temporary culture of parent clams, spawning of the parent clams, trochophore hatching, D-larvae and post-larvae culture and juvenile clam culture. Microbial preparations are thrown in during the process of temporary culture of the parent clams, a chitosan decontamination agent is thrown in during the process of spawning of the parent clams, the microbial preparations are thrown in during the course of trochophore hatching, microalgae preparations are thrown in during the course of D-larvae and post-larvae culture, and juvenile clams are fed with tetraselmis in the period of juvenile clam culture till grown-up cyclinasinensis is harvested. Purification substances are thrown in the whole period from temporary culture of the parent clams to larvae culture and juvenile clam culture for purifying meat, pollution of contaminants is avoided from the source, healthiness, greenness and non-pollution in the culture process are guaranteed, the contaminants such as heavy metal, petroleum and nitrofuran derivatives in bodies of the grown-up cyclinasinensis are reduced significantly, and good effect on removal of escherichia coli, sphingomonaspaucimobilis, vibrio, aeromonassalmonicida and the like is achieved, and the grown-up cyclinasinensis completely conforming to food security can be cultured.

The invention discloses an ecological Japanese sea horse breeding method. The ecological Japanese sea horse breeding method comprises the steps of building an ecological sea horse breeding pond one week before breeding, cultivating microalgae in the built ecological breeding pond, and measuring and controlling water quality to reach newborn sea horse breeding indexes before newborn sea horses are bred. When the newborn sea horses is younger than 15 days, the released density is 1000-1200 tail / m<3>, and baits are composed of rotifer, copepods larvae, chlorella, photosynthetic bacteria, tetraselmis and fairy shrimps which are just incubated. During 15-30 days, the released density is 600-1000 tail / m<3>, baits are composed of fairy shrimps, copepods larvae and shrimp seeds, and the baits are fed into the breeding pond four times per day. A sea horse juvenile fish breeding stage comprises the step of enabling the water quality to reach juvenile fish breeding indexes before breeding. During 30-40 days, the released density is 600-800 tail / m<3>, and the baits are fed into the breeding pond three times per day. A sea horse adult fish breeding stage comprises the step of enabling the water quality to reach sea horse adult fish breeding indexes. The released density of sea horse adult fish is 200-400 tail / m<3>, baits are fresh and alive shrimps, and the baits are fed into the breeding pond three times per day. The ecological Japanese sea horse breeding method has the advantages of reducing production cost.

The invention discloses a microbial water purifying agent for hairy crab culture water and a preparation method of the agent. The preparation method comprises the following steps: adding 20-40 parts by weight of magnetic silicon spheres of which the surface is modified with C8 and C18 alkyl chains to 200-400 parts by weight of water; adding 130-180 parts by weight of microbial reagents which include 30-60 parts of synechococcus, 30-50 parts of rhodospirillum, 10-30 parts of nitrococcus, 10-30 parts of nitrospina, 10-20 parts of thiobacillus denitrificans, 10-20 parts of raw iron bacteria, 3-5 parts of beggiatoa, 1-3 parts of vitreoscilla, 30-70 parts of phytoflagellate, 30-50 parts of zooflagellate, 20-50 parts of tetraselmis and 20-50 parts of chlorella; next, adding 60-80 parts by weight of beef extract peptone agar culture medium for culturing. The microbial water purifying agent is put into a hairy crab culture pond to purify water in the culture pond.

The invention discloses a method for cultivating Tetraselmis subcordiformis by utilizing winery wastewater. The technique can treat wine wastewater at low cost, change wastes into valuable substances, and obtain Tetraselmis subcordiformis with important bait value, thereby implementing multiple purposes. The traditional Tetraselmis subcordiformis culture solution has fewer nutrients and uses an open cement track tank for culture, and thus, has the defects of low growth speed, low yield, high investment, high tendency to pollution and low benefit. At present, a water tower made from high-density polyethylene with the volume of 3 cubic meters, which has the advantages of low cost and low tendency to pollution, can be reutilized and is easy to operate, is adopted; and wine wastewater, urea sea mud extracting solution, soil extracting solution, cattle manure leaching solution, L-cysteine, casein peptone, medical stone powder, sodium dihydrogen phosphate, S3 vitamin, human urine and other nutritional ingredients are added into the culture medium, and the organic fertilizer and inorganic fertilizer are mixed for use, so that the nutrition is more comprehensive and balanced, thereby greatly enhancing the growth speed of the Tetraselmis subcordiformis and enhancing the yield by 350%.