The invention discloses a breeding method for improving disease resistance of soft-shelled turtles, wherein the method includes the following steps: S1, building a breeding pond: arranging soil at thebottom of the breeding pond, sterilizing, applying an organic fertilizer, planting aquatic plants, injecting clear water and adjusting the pH value of the clear water; S2, putting the soft-shelled turtles and a bait material: selecting robust and young soft-shelled turtles, sterilizing, putting into the breeding pond with the female-male proportion of (3-5):1, and putting the bait material for 1-3 times daily; and S3, carrying out daily management: carrying out tour inspection of the breeding pond in the morning and evening, timely cleaning residual baits, and exchanging water timely. The breeding method for improving disease resistance of the soft-shelled turtles has the advantages of high utilization rate of resources, good self-regulation of the water body, less water exchange amount,reduction of breeding cost and green environmental protection, the soft-shelled turtles have the advantages of low incidence rate, good disease resistance, strong immunity, fast growth and development, short breeding cycle, good meat quality and high nutritional value, and the economic benefits of soft-shelled turtle breeding is increased.