The invention discloses a compound feed for jade perch and spot fish cultivation and a preparation method thereof. The compound feed is prepared from fish meal, fermented insect protein, bean pulp, fermented cotton pulp, fermented rapeseed meal, zymolytic soybean protein, corn distilled-grain protein, rice bran, wheat middling, flour, soya-bean oil, zeolite powder, salt, monocalcium phosphate, choline chloride, glycine, alanine, glycine betaine, DMPT (Dimethylpropiothetin), pine needle meal, hawthorn berry powder, L-carnitine, bile acid, econazde XP, curcumin, tea polyphenol, multivitamin and composite mineral salt. The compound feed is prepared by crushing, gelatinizing, pelleting and drying raw materials, and the like. The compound feed improves the survival rate of jade perch and spot fish, promotes the healthy cultivation, improves the digestion, absorption and conversion of fat in a fish body, effectively prevents the generation of fatty liver, greatly reduces intestinal fat accumulation, efficiently increases the muscle accumulation of jade perch and spot fish, increases the meat rate of the finished jade perch and spot fish products, and improves the flavor.