Microbial preparation for improving bottom ecological environment of prawn pond and preparation method thereof

A microbial preparation and ecological environment technology, applied in the field of microbial preparations, can solve the problems of shortening the service life of aquaculture areas, large environmental pollution of shrimp farming, waste of water resources, etc., achieving a strong feeding effect, reducing the occurrence of shrimp diseases, The effect of promoting reproduction and growth

Inactive Publication Date: 2014-04-02
上海绿博生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The current treatment methods for shrimp ponds, such as changing water and dredging, have caused serious pollution to the environment of shrimp farming, and brought negative effects such as cross-infection, waste of water resources, and shortened service life of breeding areas.

Method used

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  • Microbial preparation for improving bottom ecological environment of prawn pond and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The steps of preparing the microbial preparation improving the ecological environment at the bottom of the shrimp pond are as follows:

[0051] Step 1, production of seeds

[0052] Put the Bacillus subtilis, Bacillus licheniformis, photosynthetic bacteria and nitrifying bacteria purchased from the China Agricultural Microorganism Culture Collection and Management Center respectively in the culture medium, and cultivate them at 2°C for 48 hours, until the surface of the culture medium is covered with a lawn and whitened. Take it out when it turns yellow, and then store it in a refrigerator at 2°C for later use;

[0053] Step 2, strains alone and compound culture

[0054] Weigh the strains cultivated in step 1 according to the following weight percentages: 20% of Bacillus subtilis, 25% of Bacillus licheniformis, 25% of photosynthetic bacteria, and 30% of nitrifying bacteria.

[0055] (1) Cultivation of photosynthetic bacteria:

[0056] At 28°C, the photosynthetic bacte...

Embodiment 2

[0075] The steps of preparing the microbial preparation improving the ecological environment at the bottom of the shrimp pond are as follows:

[0076] Step 1, production of seeds

[0077] Put the Bacillus subtilis, Bacillus licheniformis, photosynthetic bacteria and nitrifying bacteria purchased from the China Agricultural Microorganism Culture Collection and Management Center respectively in the culture medium, and cultivate them at 38°C for 72 hours. It can be taken out when it turns yellow, and then stored in a 4°C refrigerator for later use;

[0078] Step 2, strains alone and compound culture

[0079] The strains cultured in step 1 are weighed according to the following weight percentages: 30% of Bacillus subtilis, 25% of Bacillus licheniformis, 20% of photosynthetic bacteria, and 25% of nitrifying bacteria.

[0080] (1) Cultivation of photosynthetic bacteria:

[0081] The culture temperature was 35° C., and the photosynthetic bacteria were inoculated under aseptic cond...

Embodiment 3

[0099] Experimental method: choose a Penaeus vannamei water quality standard breeding pond as the experimental pond, carry out a period of 20 days of experimental records, drop into an appropriate amount of microbial preparations described in the embodiment of the present invention 1 every day, during the experiment, use a water quality measuring instrument. Every day at 6:00 am and 3:00 pm, test and record the temperature and pH value of the experimental pond water, and at 3:00 pm, test and record nitrite, nitrogen ammonia, total alkalinity, dissolved oxygen content, sulfide Hydrogen content, etc. And record the daily feeding amount and the change of the water color of the shrimp pond every day.

[0100] Experimental judgment criteria: as shown in Table 1.

[0101] Table 1. List of water quality standards for Penaeus vannamei

[0102] Environmental parameters

Appropriate index

Water quality to be treated

temperature

28-31℃

below 19°C

...

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Abstract

The invention discloses a microbial preparation for improving a bottom ecological environment of a prawn pond and a preparation method thereof. The microbial preparation takes the total preparation as a standard, and comprises a strain containing 5-50% of bacillus subtilis, 5-40% of bacillus licheniformis, 5-50% of photosynthetic bacteria and 3-40% of nitrifying bacteria. The preparation method comprises the following steps: production and seed production, singly cultivating the photosynthetic bacteria and the nitrifying bacteria to obtain a bacterium solution; carrying out composite cultivation on the bacillus subtilis and the bacillus licheniformis to obtain the bacterium solution; cultivating and fermenting the obtained bacterium solution and then centrifuging to obtain a high-concentration bacterium solution; mixing the bacterium solution with a special carrier; drying and crushing, so as to obtain the microbial preparation. By adopting the microbial preparation disclosed by the invention, the bottom organisms of the pond and the chemical oxygen consumption can be effectively reduced, an excellent bottom ecological environment is provided for the prawn, the survival rate is improved, and a disease is reduced. By adopting the method, metabolite can be produced in a fermentation manner, and the content of beneficial viable organisms and active enzyme in the product is increased.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a microbial preparation capable of improving the ecological environment at the bottom of a prawn pond and a preparation method thereof. Background technique [0002] As the consumption level of Chinese residents is getting higher and higher, the requirements for the diversity and nutritional value of food are also getting higher and higher. Because of its rich nutrition, soft meat, easy digestion, rich in magnesium, prawns are beneficial to the cardiovascular system, can prevent arteriosclerosis, and can also dilate coronary arteries, which is beneficial to prevent high blood pressure and myocardial infarction. It is deeply loved by consumers. favorite. The sharp increase in market demand for prawns has also led to the rapid development of my country's prawn farming industry. [0003] However, high-density farming has brought great challenges to shrimp farming. The main problem is tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C02F3/34C12R1/125C12R1/10
Inventor 江瀚陆瑛
Owner 上海绿博生物科技发展有限公司
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