The invention discloses an aqueous anti-doodling paint, which consists of antifouling functional materials, assistants, glass powder filling and water, wherein the antifouling functional materials comprise pure acrylic ester emulsion, wax emulsion and fluorine-type assistant; and the assistants comprise dispersant, wetting agent, film-forming assistant, anti-freezing agent, defoaming agent, thickening agent, mildew-proof agent and preservative. The preparation method of the invention comprises steps of premixing, grinding and dispersing, emulsion adding, assistant adding, packing and the like. The aqueous anti-doodling paint has good antifouling and anti-doodling functions, wherein the VOC content is 52 to 65 g/L, the content of benzene series substance is less than 300 mg/kg, and the contents are far below the index values of volatile organic compounds of solvent products; and the aqueous anti-doodling paint is suitable for indoor space. Moreover, the paint film is compact; meanwhile the paint has the capacities of resisting seepage and reducing the adhesion of pollutants; and particularly aiming at the substances such as children water color pens, crayons, color pencils, ball pens, ink, coffee, tomato sauce, tea juice, shoe print and the like, the pollutants can be easily scrubbed.