374 results about "Distillers grains" patented technology

The invention provides a method and a device for controlling a process of feeding distillers' grains into a retort for distillation, a storage medium and electronic equipment, and is applied to the technical field of automation control. According to the method, by distinguishing different batches of vinasse material property data and steam thermal physical property data and inputting the data into the pre-trained retort feeding temperature field model, the temperature field data in the retort feeding process can be accurately predicted. Temperature field data and vinasse material property data are combined, so that the steaming height, timing, steam flow and pressure can be effectively controlled, and the steaming process is optimized. Thirdly, inputting the steaming end temperature field data and the vinasse material property data into the distillation wine taking model to obtain distillation wine taking data so as to control the steam flow and pressure in the distillation process and realize accurate control on the steaming process and the distillation process of the vinasse, so that the production efficiency and the vinasse quality stability of the solid-state-method white spirit are effectively improved.

The vinasse concentrated liquor is a stream of concentrated liquor generated in the fermentation process of the vinasse of the Maotai-flavor liquor, and is rich in organic substances such as alcohols and esters. The invention relates to the technical field of fertilizer fermentation, and discloses a fertilizer production system for fermentation treatment of Maotai-flavor liquor vinasse concentrated liquor. According to the system, a fermentation processing area is divided into a plurality of fermentation units, and Maotai-flavor liquor vinasse concentrated liquor component data, organic auxiliary material ratio data and environment monitoring data in each unit are collected to form a multi-source fermentation data set. Based on the data set, a vinasse concentrated liquid metabolic activity index and an auxiliary material decomposition efficiency index are constructed, and an initial fermentation potential value is generated through a dual-threshold response mechanism and a dynamic attenuation model. And carrying out collaborative correction on the initial fermentation potential value according to the material diffusion relevance of the adjacent fermentation units to obtain a corrected fermentation potential value. And performing descending sorting on the corrected fermentation potential values to generate a fermentation priority sequence, and further driving an execution strategy of a fermentation control unit based on the sequence. According to the system, accurate control over the fermentation process is achieved, and scientificity and efficiency of fertilizer production through fermentation treatment of the Maotai-flavor liquor vinasse concentrated liquor are improved.

The invention relates to the technical field of microbial fermentation feeds, in particular to a method for producing a protein feed through solid-state fermentation of distiller's grains based on Trichoderma harzianum pretreatment. According to the method, the vinasse is pretreated by the crude enzyme prepared from trichoderma harzianum, and the formula and fermentation conditions of an enzyme production culture medium are optimized, so that the filter paper enzyme activity of cellulase is remarkably improved. By adding the surfactant into a fermentation system and optimizing the variety of the surfactant, the yield of the fermented monosaccharide is improved, and the problem of musty taste in a traditional fermented product is solved while the degradation efficiency of lignocellulose is improved. Furthermore, a synchronous saccharification and fermentation process is adopted, vinasse is fermented through compounded saccharomycetes and crude enzyme liquid, the trichoderma harzianum fermented protein feed is prepared and obtained, digestion and absorption of animals are easier, lactic acid fermentation is conducted on the trichoderma harzianum fermented protein feed again through lactobacillus fermentum, the flavor of the feed is improved, the bitter taste of the feed is reduced, and the sour taste is improved. The method realizes efficient utilization of vinasse resources.

The invention discloses a mulberry leaf-vinasse fermentation composition which comprises the following raw materials: vinasse, mulberry leaves and a complex microbial inoculant, the complex microbial inoculants comprise saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum. The mulberry leaf-vinasse fermentation composition is obtained by performing anaerobic solid state fermentation on the raw materials, namely crushing vinasse and mulberry leaves to 20-50 meshes, uniformly mixing, adjusting the water content of the raw materials to be 50-70wt%, inoculating a complex microbial inoculant, uniformly stirring, filling into a breathing bag, sealing at 37 + / -0.5 DEG C, and fermenting for 4-6 days, thereby obtaining the mulberry leaf-vinasse fermentation composition. According to the mulberry leaf-vinasse fermentation composition, the mulberry leaves and the vinasse are mixed, then the complex microbial inoculant is inoculated for solid-state fermentation, the pH value of the prepared mulberry leaf-vinasse fermentation composition is remarkably reduced, meanwhile, the lactic acid content is remarkably increased, the antinutritional factor content is reduced, true protein is well preserved, the antioxidant activity is more reserved, and the fermentation effect of the mulberry leaves and the vinasse is improved; the feeding value of the mulberry leaves and the vinasse is effectively improved.

The invention discloses a modified vinasse biochar-based nitrogen fertilizer and a preparation method and application thereof, and the preparation method comprises the following steps: (1) drying and crushing vinasse to obtain vinasse particles; (2) mixing and dipping the vinasse particles and a phosphoric acid solution, and then drying to obtain modified vinasse; (3) under a protective atmosphere, pyrolyzing the modified vinasse to obtain modified charcoal; (4) adding an ammonium chloride solution into the modified biochar, adjusting the pH value, heating and oscillating, then taking out the modified biochar after adsorption, and drying to obtain the modified vinasse biochar-based nitrogen fertilizer. The prepared modified vinasse biochar-based nitrogen fertilizer is applied to the field of chemical fertilizer preparation, resource utilization of a wine brewing byproduct vinasse is achieved, the risk of environmental pollution is reduced, the vinasse is made into the biochar-loaded nitrogen fertilizer, technical reference is provided for effective utilization of the nitrogen fertilizer, and the modified vinasse biochar-based nitrogen fertilizer has practical significance in promotion of industrial green cycle development.

The invention discloses a treatment method of heavy metal stabilized red mud based on multielement solid waste synergy and application of the treatment method. According to the method, four solid wastes including coal mine area soil, vinasse, acidic biochar and garden soil are adopted as stabilizers, the water extraction state content of the heavy metals in the red mud is reduced in a multi-element combined adding mode, simultaneous stabilization of multiple heavy metals in the red mud is achieved, and the highest reduction rate reaches 99.0%. Harmless treatment of the red mud and resource utilization of solid waste such as coal mine area soil, vinasse, acidic charcoal and garden soil are achieved, and the method has the advantage of treating waste with waste. The method is easy to operate, low in cost, free of complex equipment and easy to industrially popularize and apply. The treated red mud can be used in the fields of building materials, roadbed materials or soil amendments and the like, and cyclic utilization of resources is realized.

The invention discloses vinasse activated carbon and a preparation method thereof. The preparation method comprises the following steps: S1, drying and crushing vinasse, adding wine brewing wastewater, mixing, carrying out hydrothermal carbonization, filtering, washing and drying to obtain hydrothermal carbon; s2, adding an activating agent into the hydrothermal carbon in the S1 for dipping, and drying to obtain dipped hydrothermal carbon; s3, performing high-temperature activation on the soaked hydrothermal carbon in the step S2, cooling, washing and drying to obtain the hydrothermal carbon. The wine brewing wastewater is used for hydrothermal carbonization, the yield of activated carbon can be increased, the adsorption performance of the activated carbon can be improved, and the method is low in production cost, environmentally friendly, high in resource utilization rate and good in industrial application prospect.

The invention relates to a protein feed prepared from biologically refined distiller's grains and a preparation method thereof, and relates to the technical field of bioengineering and feed resource development. The method comprises the following steps: (1) sequentially carrying out acid pretreatment and double-enzyme synergistic hydrolysis on the white spirit vinasse to obtain hydrolysate containing fermentable sugar; (2) adding a candida utilis yeast liquid and urea, and carrying out carbon-nitrogen synergistic liquid fermentation to obtain a fermentation liquid with the viable count of candida utilis greater than or equal to 4.0 * 10 < 8 > CFU / mL; and drying. According to the method, the white spirit vinasse is subjected to diluted acid high-pressure pretreatment and double-enzyme synergistic hydrolysis, then candida utilis is utilized for carbon-nitrogen synergistic liquid fermentation, efficient utilization of glucose and xylose is achieved, and the mycoprotein yield is larger than or equal to 0.70 g / L / h; according to the method, all-component high-value utilization of the distiller's grains is realized, and a process path with industrial production potential is provided for preparing the protein feed by biorefining the distiller's grains.

The invention discloses soft Maotai-flavor liquor and a preparation method thereof. The method comprises the following steps: raw material preparation, yeast material preparation, sand feeding and feeding, sand insertion and feeding, distillation and aging. In the raw material preparation step, sorghum is crushed and then mixed with hot water and white spirit vinasse, and grain fermentation and water airing treatment are carried out; mixing the broomcorn material for sand feeding with Maotai-flavor yeast powder and an enzyme catalyst, and fermenting; in the sand insertion and feeding step, sand insertion fermented grains are mixed with a sorghum material for sand feeding, Maotai-flavor yeast powder, a functional agent and a synergist, and fermentation is performed again; in the distillation step, the sand-inserted fermented grains and steamed rice husks are mixed and then distilled; and finally, aging for a period of time to obtain the soft Maotai-flavor liquor. Compared with the prior art, efficient preparation of the soft Maotai-flavor liquor can be achieved, the taste of the obtained soft Maotai-flavor liquor is further blended and improved, and the soft Maotai-flavor liquor is higher in quality.

The invention provides a strain of saccharomyces cerevisiae, the strain of saccharomyces cerevisiae is named as Saccharomyces cerevisiae LDGJ4, the strain of saccharomyces cerevisiae is preserved in China Center for Type Culture Collection on March 19, 2025, and the preservation number is CCTCC NO: M 2025529. The Saccharomyces cerevisiae provided by the invention is used for fermenting Luzhou-flavor distiller's grains, the Saccharomyces cerevisiae provided by the invention shows good adaptability in the fermentation process, and distiller's grain fermented feed with rich nutritional value and good palatability can be prepared. The problems of waste materials, pollution and the like in the white spirit brewing industry are solved; meanwhile, the additional economic value is increased.

The invention relates to a small cellar sauced vinasse hydrolysis peptide with alpha-glucosidase inhibitory activity and a preparation method thereof. Five small cellar Maotai-flavor vinasse hydrolytic peptides with alpha-glucosidase inhibitory activity are prepared through vinasse protein preparation, enzymolysis, separation and purification and identification, and the sequences of the five small cellar Maotai-flavor vinasse hydrolytic peptides are AAPFYAL, LAYPQ, IRLPFNQI, INPALPW and ATPVFL respectively. The molecular docking analysis result shows that the interaction between the vinasse polypeptide and the amino acid residues of the alpha-glucosidase active pocket is mainly based on hydrogen bonds, electrostatic binding hydrophobic interaction and pi-pi interaction. Pharmacophore construction and matching analysis show that the vinasse polypeptide has a common characteristic structure composed of a positive ion center and three hydrogen bond donors and plays a key role in enzyme inhibition. The small cellar sauced vinasse polypeptide with alpha-glucosidase inhibitory activity prepared by the invention can be used for developing health-care foods and medicines for reducing blood sugar.

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention relates to an intelligent adjusting system and method for a paddle drying device. The system comprises a sensing unit and an adjusting unit. The sensing unit comprises a viscosity sensor, a temperature sensor and a fluidity sensor, the adjusting unit is in communication connection with the sensing unit, the vinasse drying state index and the change trend thereof are calculated in real time based on viscosity parameters, temperature parameters and fluidity parameters, and the change trend of operation parameters of the paddle drying device is monitored in real time; and when the vinasse drying state index reaches a preset adjustment threshold value, an instruction for increasing the stirring frequency or reducing the stirring frequency is sent to the control module. According to the invention, the drying state of the vinasse can be evaluated more accurately. The accurate evaluation avoids the problem of low drying efficiency caused by single parameter or inaccurate evaluation in the traditional method. The dynamic adjusting mode of the adjusting unit can optimize the stirring frequency according to the real-time drying state, excessive drying or insufficient drying is avoided, and therefore the drying efficiency is remarkably improved.

The application provides a kind of flower cultivation soil based on waste pit mud, distiller's grains and a preparation method thereof, which comprises the following steps: step S1: after drying, ore grinding, the waste pit mud is screened to obtain large block stone with particle size greater than 4mm, large particle sandstone with particle size of 2-4mm and purple mud with particle size less than 2mm; step S2: the purple mud and the large particle sandstone are mixed according to a mass ratio of 7:3-8:2 to obtain pit mud without stone; step S3: the pit mud without stone and the distiller's grains are mixed according to a mass ratio of 7.5:2.5-8.5:1.5 to obtain the cultivation soil base; step S4: lime is added to the cultivation soil base to adjust the pH to 6-7, and an appropriate amount of surfactant is added to obtain the flower cultivation soil. The method can obtain the flower cultivation soil with suitable porosity and rich organic matter, which can effectively promote the growth of plants and realize the recycling of waste pit mud and distiller's grains.

The invention discloses a white spirit fermentation byproduct recovery device, and relates to the technical field of white spirit equipment.The white spirit fermentation byproduct recovery device comprises a first supporting frame, a stirring and filter pressing structure is arranged on the upper portion of the first supporting frame, a quantitative discharging structure is arranged on the upper portion of the stirring and filter pressing structure, and a crushing device is arranged on the upper portion of the quantitative discharging structure; a purification structure is arranged at the lower part of the stirring and filter pressing structure. According to the by-product recovery device for Baijiu fermentation, manual feeding is combined with mechanical automation, vinasse is rapidly crushed by a crushing roller to prevent blockage, quantitative discharging is achieved, a leavening agent is synchronously mixed, the process is simplified, and material stability is guaranteed; during filter pressing, a servo motor cooperates with multiple structures, uniform pressure is applied to improve efficiency, scraping strips clear residues, stirring blades eliminate dead angles, and buffer design protects equipment; liquid is primarily filtered and deeply filtered, the angle of the movable bin is adjusted to ensure thorough purification, manpower is greatly reduced through automation, the purified liquid can be recycled, and the treatment efficiency and the resource utilization rate are integrally improved.

The utility model provides a device for producing organic fertilizer by utilizing vinasse waste, which relates to the field of organic fertilizer production and comprises a crushing box (10), a screening box (20), a fermentation box (30) and a collecting box (40), the crushing box (10) is arranged on one side of the screening box (20), and a crushing component is arranged in an inner cavity of the crushing box (10); a screening barrel (21) is arranged in an inner cavity of the screening box (20), and a filter screen (211) is arranged on the bottom face, located in the inner cavity of the screening box (20), of the screening barrel (21). The bottom end of the crushing box (10) is communicated with an inner cavity of the screening barrel (21) through a material guide pipe (14); a fermentation box (30) is arranged at the bottom end of the screening box (20), and a fermentation tank (31) and a stirring assembly are arranged in the fermentation box (30); and the collection box (40) is arranged on the lower side of the fermentation box (30). The device not only can prevent vinasse waste from splashing, but also can realize water guide, ventilation and stirring of compost in the fermentation process.

The invention provides vinasse dietary fiber with blood fat reducing activity and a preparation method of the vinasse dietary fiber. The vinasse dietary fiber with relatively high purity is obtained by performing alkali soaking, sodium chlorite bleaching, crushing and drying on the vinasse. The prepared vinasse dietary fiber can effectively improve hyperlipidemia of obese diabetes model mice, reduce triglyceride and total cholesterol levels of serum and liver and improve liver injury, and can be applied to preparation of functional food, health care products, medicines and feeds with the blood glucose reducing effect.

The utility model relates to the technical field of wine brewing equipment, aims to solve the problems that in the prior art, the labor intensity of workers is high, yeast spreading is not uniform, yeast powder flies around, the waste of the yeast powder is large, and vinasse is easily adhered and waxy by a yeast spreading trolley, and provides an automatic yeast powder spreading trolley which comprises a hopper, a first sheet-shaped hub and a second sheet-shaped hub are arranged on the two sides of the bottom of the hopper respectively, and the first sheet-shaped hub and the second sheet-shaped hub are rotatably connected to the two sides of the bottom of the hopper; a long discharging hole is formed in the bottom of the hopper, and a control assembly used for adjusting the scattering flow is arranged outside the long discharging hole. The device has the beneficial effects that the labor intensity is low, yeast spreading is uniform, yeast powder is not easy to fly, the utilization rate of the yeast powder is high, and the yeast spreading vehicle is not easy to adhere and press glutinous rice vinasse.

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

To provide a method for increasing an amount of erinacine A produced in a method for producing erinacine A including a liquid culture step of Hericium erinaceus mycelium.SOLUTION: Provided is a method for producing erinacine A, including a liquid culture step of culturing Hericium erinaceus mycelium, a liquid medium used in the liquid culture step containing soybean flour in an amount of 0.2 w / v% or more and less than 2.5 w / v% based on the total volume of the liquid medium, further containing at least one material selected from the group consisting of ground grain husks, sake lees, and brewer's lees, and when ground grain husks are contained, the ground grain husks containing more than 0.25 w / v% based on the total volume of the liquid medium.SELECTED DRAWING: None

The utility model discloses a volatile compound collecting device, which relates to the technical field of vinasse organic fertilizer volatile compound collection and comprises a reaction kettle, a feed port is fixedly mounted at the top end of the reaction kettle, and handles are symmetrically and fixedly mounted at the top end of the reaction kettle. A collecting mechanism for collecting volatile gas is fixedly mounted in the top end of the reaction kettle, and one end of the collecting mechanism penetrates through the side wall of the reaction kettle and extends out of the reaction kettle. Through the arrangement of the collecting mechanism, volatile compounds can be rapidly collected and rapidly sampled at a fixed point, so that a worker can analyze the concentration and components of the volatile compounds so as to correspondingly process the volatile compounds and then carry out subsequent collecting work, and due to the rapid sampling mechanism, the working efficiency is greatly improved, and the working efficiency is improved. Volatile compounds in different stages can be quickly sampled according to different stages of fermentation, so that the convenience of the device in the use process is further improved.

The invention belongs to the related technical field of fermentation production, and particularly relates to a device for conveying vinasse from a fermentation tank and a use method.The device comprises a base, a plurality of rolling wheels are arranged at the bottom of the base, a rack is rotationally arranged on one side of the upper end of the base, a conveying belt is arranged on the rack, and one end of the base is fixedly connected with a material collecting shell; a rotating column is rotationally arranged on one side of an inner cavity of the material collecting shell, a plurality of blades are evenly and fixedly connected to the periphery of the rotating column, and a vinasse conveying and guiding assembly is further arranged on the machine frame. By means of the vinasse conveying and guiding assembly, when vinasse needs to be conveyed into a plurality of collecting containers, precise guiding of the vinasse conveying direction and the discharging range can be achieved, the situation that when the vinasse falls off from a conveying belt, due to the fact that the falling range of the vinasse is random, the vinasse falls out of the collecting containers is avoided, and the material receiving efficiency is improved.

The application discloses an endoglucanase mutant with acid resistance and application thereof, and belongs to the technical field of genetic engineering and enzyme engineering. The endoglucanase tCel5A is subjected to site-specific saturation mutation by PCR amplification, and n variants S45D / T55D / T59D and T88H / W255H are obtained through screening. Enzyme activity determination results show that the endoglucanase variants S45D / T55D / T59D and T88H / W255H have enzyme activities of 8828.7 U / mg prot and 5206.7 U / mg prot respectively under acidic conditions, and the degradation rates of the endoglucanase variants S45D / T55D / T59D and T88H / W255H on distillers' grains are 53.53% and 50.47% respectively under a solid-liquid ratio of 1:30, so that the endoglucanase variants have strong acid resistance and degradation capacity on plant cellulose, and have great application value in agriculture and brewing.

The present application relates to the technical field of feed processing, and particularly relates to a preparation method of Maotai-flavor distiller's grains fermented feed, which comprises the following steps: drying treatment based on the moisture content of Maotai-flavor distiller's grains to obtain basic distiller's grains; determining the strain ratio of brewer's yeast, bacillus subtilis and aspergillus niger based on the tannin content of the basic distiller's grains to obtain a compound fermentation inoculant; inoculating and then fermenting; determining state evaluation parameters based on the total polyphenol accumulation rate, the dissolved oxygen consumption rate and the microbial activity index during the fermentation process, and performing qualified evaluation, and dynamically adjusting when unqualified; determining the fermentation end point based on the tannin degradation rate and the total polyphenol accumulation rate; and mixing the fermented distiller's grains with the basic feed at a mass ratio of 1% after low-temperature drying of the fermented distiller's grains. The present application realizes three-layer control of raw material evaluation, process control and end point determination, significantly improves the stability and content of functional active ingredients in the product, reduces the tannin and safety risk, and has a good intestinal health improvement effect on animals.

The invention discloses vinasse bread premixed flour and a preparation method thereof, and belongs to the technical field of bread premixed flour. The premixed flour comprises the following components in percentage by weight: 40%-60% of vinasse powder, 30%-40% of high gluten wheat flour, 5%-15% of rye flour, 3%-8% of vital gluten, 0.5%-1.5% of edible corn starch and 1%-2% of a bread improver. The bread improver is prepared by compounding mono-and diglycerides of fatty acids, diacetyl tartaric acid ester of mono-and diglycerides, a plurality of enzyme preparations and vitamin C. The preparation method comprises the steps of raw material pretreatment, mixing and the like. Compared with the prior art, by means of an optimized formula system, the technical problems that a dough system is degraded, the taste is hard, and aging is prone to occurring due to the fact that vinasse is added in a high proportion are successfully solved, and the finally-made bread is softer in taste and rich in elasticity, prolongs the shelf life and has high nutritive value and excellent eating quality.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The present application belongs to the technical field of processing of by-products of brewing, and specifically discloses a method and device for reducing viscosity of distiller's grains based on enzyme treatment, which comprises the following steps: S1, dispersing the distiller's grains of liquor and mixing the auxiliary materials by means of a stirrer; S2, adjusting the pH of the uniformly dispersed and mixed distiller's grains by means of ammonia water, and then adding enzyme hydrolysate, and performing enzymolysis for 24 hours; and S3, further adding decomposition enzyme to perform enzymolysis into sugar. The method and device for reducing viscosity of distiller's grains based on enzyme treatment are used to disperse the distiller's grains and mix the auxiliary materials by means of the inner rotation and outer rotation modes of the stirrer, and to reduce the viscosity of the distiller's grains by means of a two-step enzyme hydrolysis method, so that the viscosity of the distiller's grains is reduced and the saccharification rate of the distiller's grains is improved, thereby laying a foundation for further fermentation of the distiller's grains to produce protein.

The application discloses a method for preparing bacterial cellulose by using supernatant after protein extraction from distiller's grains and application thereof. The method comprises the following steps: S1, performing protein extraction treatment on a distiller's grains hydrolysate to obtain supernatant after protein extraction from the distiller's grains, then mixing the supernatant with a Henley-Schramm culture medium, adjusting the pH value to 5.5-6.8 to obtain a compound culture medium; S2, inoculating a Komagataeibacter xylinus seed liquid into the compound culture medium, performing shallow static culture, collecting a surface bacterial cellulose membrane, and performing purification to obtain the bacterial cellulose. It is found by experiments that, under the same compound ratio, the yield of the bacterial cellulose prepared by using the supernatant after protein extraction from the distiller's grains is higher than that of the bacterial cellulose prepared by directly using the distiller's grains hydrolysate without protein extraction, and the crystallinity of the obtained bacterial cellulose product is also higher. The preparation process is simple, and can provide an effective way for low-cost industrial production of the bacterial cellulose and efficient utilization of distiller's grains waste.

The utility model discloses a vinasse matrix mixing device. A spiral blade and a lifting plate are arranged on the inner wall of a horizontally-arranged rotating drum. An annular columnar material collecting barrel is arranged above the front end of the rotary barrel, a cylinder is rotationally mounted in the annular columnar material collecting barrel, a stirring shaft is arranged in the cylinder, and the central axes of the material collecting barrel, the cylinder and the stirring shaft coincide. At least three layers of vertically spaced connecting frames are arranged in the cylinder, the stirring shaft penetrates through the connecting frames, and the outer edges of the connecting frames are connected with the inner side wall of the cylinder and covered with the cutting net. The top of the material collecting barrel is open and provided with a mounting frame, a stirring motor and at least two first quantitative feeding mechanisms are arranged above the material collecting barrel, a stirring shaft is in driving connection with the motor, and the output ends of the first quantitative feeding mechanisms are arranged above the cylinder. And the bottom of the material collecting barrel is connected with a conical hopper which is connected with an inclined feeding pipe and extends into the rotating barrel. A second quantitative feeding mechanism is arranged outside the material collecting barrel, and the output end is arranged in the conical hopper. A water supply pipe is arranged in the drum, two ends of the drum are connected with a support frame, a plurality of annular sprayers are arranged on the drum, and a flow pump is arranged outside the drum for supplying water. According to the device, continuous feeding and discharging mixing and mixing uniformity of all materials of the vinasse matrix can be realized.