The application discloses a method for preparing
bacterial cellulose by using supernatant after
protein extraction from distiller's grains and application thereof. The method comprises the following steps: S1, performing
protein extraction treatment on a distiller's grains
hydrolysate to obtain supernatant after
protein extraction from the distiller's grains, then mixing the supernatant with a Henley-Schramm culture medium, adjusting the pH value to 5.5-6.8 to obtain a compound culture medium; S2, inoculating a Komagataeibacter xylinus seed liquid into the compound culture medium, performing shallow static culture, collecting a surface
bacterial cellulose membrane, and performing purification to obtain the
bacterial cellulose. It is found by experiments that, under the same compound ratio, the yield of the bacterial
cellulose prepared by using the supernatant after protein extraction from the distiller's grains is higher than that of the bacterial
cellulose prepared by directly using the distiller's grains
hydrolysate without protein extraction, and the
crystallinity of the obtained bacterial
cellulose product is also higher. The preparation process is simple, and can provide an effective way for low-cost industrial production of the bacterial cellulose and efficient utilization of distiller's grains waste.