287 results about "Sporolactobacillus" patented technology

The invention relates to a prebiotic peptide biological feed additive and a preparation method and application thereof. The prebiotic peptide biological feed additive is prepared by mixing and fermentation of seed solution with one or more of bacillus, yeast and lactic acid bacteria in plant protease hydrolysate. After animal and plant protein is subjected to enzymolysis at constant or changeable temperature, the seed solution with one or more of bacillus, yeast and lactic acid bacteria is added for fermentation according to a certain proportion, and the fermented solution is concentrated and dried to obtain a powdery product. The problems of high content of bitter peptides and poor palatability of enzymolysis processes using acid, alkali and protease can be solved. The immune function of animals can be stimulated by active peptides in the prebiotic peptide biological feed additive, the immunity of the animals can be improved, the intestinal environment of the animals can be improved by probiotics, the resistance of the animals to a variety of diseases can be enhanced, the feed conversion rate can be increased, and the breeding cost can be reduced.

The invention relates to a microbe soil repairing additive and process for preparation. The process for preparation comprises that liquid microbe fertilizer containing photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria are absorbed to one or a plurality of natural organic fertilizers as grass peat and zeolite powder or the like, to be mixed proportionally. The invention further relates to the application of the microbe soil repairing additive for repairing soil. The invention can lead useful microbe into soil, uses microbe technique to transfer, absorb, degrade, and convert the contaminant in soil, adjust soil pH value, eliminate soil blocking, and restrain soil-borne disease, thereby improving soil structure, to return soil to original ecologic structure. And the viable bacteria group of the invention is planted in plant root, to quickly grow and deeply insert plant root, thereby improving yield, quality and level of agricultural product, as one ideal fertilizer of organic agriculture, with wide application in agriculture continuous development.

The invention discloses a direct feeding viable bacteria preparation, wherein the viable bacteria content for each composition being, bacillus 1.0X10#+[9]-25X10#+[9]cfu/g, saccharomycete 1.0X10#+[9]-10X10#+[9]cfu/g, lactic acid bacteria 1.0X10#+[8]-10X10#+[8]cfu/g, the invention also discloses the process for preparing the viable bacteria preparation.

The invention relates to a composite microbe fertilizer. The composite microbe fertilizer is prepared by 25-35 wt% of vegetable garden soil, 5-10 wt% of active functional bacteria, 5-15 wt% of mineral powder, 25-30 wt% of an organic fertilizer, 15-20 wt% of an inorganic composite fertilizer, and water; wherein the sum of the components is 100%. The active functional bacteria comprise lactic acid bacteria, bacillus subtilis, Bacillus megaterium, trichoderma, aspergillus niger, monascus, Trichoderma harzianum, aspergillus aculeatus and yeast, the mineral powder is a mixture of one or more of peat, zeolite, vermiculite, montmorillonite, calcium carbonate and expanded perlite, fineness of the mineral powder is less than or equal to 200 meshes, the organic fertilizer contains decomposed manure, a rapeseed cake fertilizer, and straw, and the weight ratio of the manure to the rapeseed cake fertilizer to straw is 5.5-7.5:2.5-3.5:0.5-1.5.

The invention discloses a method for producing a microorganism feed additive by utilizing probiotics and mixed meal. The method comprises the following steps: adding water into fermentation substrates respectively, stirring materials, and then cooking and sterilizing; adding a mixed bacterial liquid into the sterilized fermentation substrates and stirring uniformly after the temperature is lowered to 28-35 DEG C, and carrying out aerobic fermentation under constant temperature; introducing a lactic acid bacterium after the fermentation substrates are fermented and mixed, and then carrying out secondary fermentation; and drying materials after fermentation at a lower temperature, and crushing so as to obtain a microorganism feed additive finished product. The fermentation substrates comprise germ meal, corn spray slurry fibers, rapeseed meal and rice bran meal; the rice mixed bacterial liquid is obtained through mixing bacterial liquids of candida utilis, bacillus licheniformis and cryytococcus neoformans; and the lactic acid bacterium is lactobacillus plantarum, and the inoculum size is 5-10% of the weight of the fermentation substrates.

The present invention provides a microbial fermented composite antibiotic-free feed containing natural antibacterial components. The feed comprises a fermented product based on the united segmented fermentation of aerobic-anaerobic composite strains, composite natural antibacterial components, a non-plant source protein/polypeptide compound nutrient supplement agent, and an additive comprising atleast a soybean protein amino acid/polypeptide compound chelate. The composite strains comprises at least lactic acid bacteria, bacillus subtilis, yeast selected from candida utilis or saccharomyces cerevisiae, and enterococcus faecalis. The fermented product based on the united segmented fermentation is prepared by carrying out secondary fermentation on fermentation raw materials through the aerobic-anaerobic composite strains. The combination of the microbial fermented feed and the natural antibacterial components is beneficial to the balance of intestinal flora of animals, and can prevent the dysbacteriosis of digestive tracts, improve the immunity functions of animal organisms, reduce or replace the use of feed antibiotics, and significantly improve the digestion and absorption efficiency of the feed and the meat quality of animals.

The invention relates to the field of feed, in particular to a compound premix feed of 1% green laying hens in laying period. The compound premix feed of 1% green laying hens in laying period is formed by uniformly mixing micronutrient substances of trace elements, vitamins, synthetic amino acids and the like, feed additives and powdered grain feed as a carrier, and is characterized by comprising active dry yeast, lactic acid bacteria, bacillus and digestive ferment. In the compound premix feed of 1% green laying hens in laying period, active dry yeast, growing element for poultry containing lactic acid bacteria and bacillus can inhibit propagation of pathogen; in wet mixed feed, active dry yeast and the like can be propagated and fermented, and partial pathogen can be inhibited and killed; and the palatability and digestibility of the feed can be improved in a certain time range. Microzyme, lactic acid bacteria and bacillus enter an alimentary tract for quick propagation, and can inhibit the growth of pathogen such as colon bacillus, salmonella and the like, thereby reducing diseases caused by pathogen, generating the effect similar to antibiotics, eliminating the damage of antibiotics to enteric microorganisms, better balancing intestinal flora, improving the immunizing power of laying hens, and improving the health level of laying hens.

The invention discloses a bioorganic-inorganic compound fertilizer containing nano-selenium and a preparation method of the bioorganic-inorganic compound fertilizer. The bioorganic-inorganic compound fertilizer containing nano-selenium comprises crop straws, selenium dioxide, animal waste, municipal sewage sludge, urea, monoammonium phosphate or diammonium phosphate, potassium chloride, a silicon-potassium fertilizer, cellulase, lactobacillus, candida utilis, bacillus subtilis, bacillus licheniformis, sodium borate, an alkaline agent, phosphoric acid, water and the like. The bioorganic-inorganic compound fertilizer is prepared by the following steps: preparing nano-selenium through reduction reaction; fermenting; burdening; crushing; pelleting; drying; sieving; packaging; and the like. According to the bioorganic-inorganic compound fertilizer, the problem that inorganic selenium is difficultly converted into nano-selenium and the problem of resource utilization of waste are simultaneously solved; the fertilizer is capable of increasing the soil fertility and preventing soil from hardening; the crop quality is improved; and the method disclosed by the invention is mild in reaction condition, simple in process, relatively low in production cost and easy for industrial production, and has good economic benefits and social benefits.

The invention belongs to the field of biological feed, and relates to a high-efficiency and low-cost fermentation feed processing method based on bean dregs and application; the processing method comprises the steps of activating a strain to obtain fermentation seed liquid of bacillus, yeast and lactic acid bacteria; mixing the bean dregs with bran or rice bran, then adding sucrose, adding cellulase, cellobiase, glucoamylase, xylanase, mannanase and beta-glucanase, mixing to obtain a primary aerobic fermentation medium, inoculating the bacillus fermentation seed liquid and the yeast fermentation seed liquid for primary aerobic fermentation, obtaining a primary fermentation mixture after fermentation, and then inoculating the lactic acid bacteria fermentation seed liquid for anaerobic fermentation under sealed conditions to obtain a finished product; an bacteria-enzyme cooperative fermentation and aerobic and anaerobic step-by-step fermentation mode is adopted, the fermentation is completed, the conversion degree of soybean peptide is high, the removal rate of trypsin inhibitor is 85% or above, each mycotoxin is lower than the feed hygiene limit standard of China; the feed is good in palatability, and favored by animals, and the economic efficiency is high.

The invention discloses a chitin active calcium fertilizer and a preparation method thereof. The fertilizer is prepared from the following materials in parts by weight: 80-120 parts of sea shell waste, 8-12 parts of soybean pulp and 8-12 parts of fermentation liquid, wherein the fermentation liquid is prepared by uniformly stirring and mixing powder containing more than 200 million per gram of anaerobic compound bacteria, with water according to a ratio of 1:(8-10); the anaerobic compound bacteria are prepared by mixing bacterium powder obtained through respectively fermenting and culturing azotobacter, lactic acid bacteria, bacillus and actinomycetes. By adopting the fertilizer, not only is the problem of shell waste in coastal shell product cultivation effectively solved and are cultivation benefits of shell products improved, but also an organic fertilizer which has various advantages such as promoting the root system development, sterilizing powerfully, improving the soil, enhancing the immunity and improving the fertilizer efficiency can be produced.

The invention belongs to the field of zymocyte microbial inocula, and particularly relates to a pickle zymocyte microbial inoculum, and a preparation method and application thereof. The pickle zymocyte microbial inoculum is composed of the following components in parts by weight: 6-8 parts of skimmed milk powder, 5-8 parts of maltose, 5-8 parts of sodium glutamate, 90-110 parts of sterilized water and zymocyte; and on the basis of the weight parts of the sterilized water, the zymocyte bacterial count is 1-2 10<5>CFU/mL, and the zymocyte is composed of Bacillus coagulans and lactobacillus plantarum in a strain ratio of 1:(0.5-3). The nitrite content in the fermented pickle finished product is lower (1.5-3.0 ug/ml), the free amino acid content is higher (2.7-4.0 g/L), and the total amount of organic acids is higher (17.0-24.0 mg/mL); and the microbial inoculum has favorable sensory quality and greatly shortens the fermentation period to 4-8 days. After being subjected to 0.1Mpa vacuum packaging, thermal sterilization in an 85 DEG C water bath for 10 minutes, addition of 20mg/100g potassium sorbate preservative and preservation for 3 months, the fermented pickle still contains the probiotic viable count of 1.0*10<3>-1.0*10<5>CFU/ml and has higher survival amount, and thus, can still maintain certain viable count after long shelf life of the product, so that the pickle has more outstanding health-care function.

The invention provides a compound bacterial agent for purifying a black and odorous water body and a preparation method thereof. The compound bacterial agent comprises the following components: actinomycetes, bacillus subtilis, nitrifying bacteria, lactic acid bacteria, candida utilis and pseudomonas aeruginosa in a weight ratio of 2:2:2:1:1:1. The invention has the following advantages: the COD content, ammonia nitrogen content and phosphate salt in the black and odorous water body can be effectively reduced, and the odor of the black and odorous water body can be eliminated; and meanwhile, by adding the ammonia nitrogen removal strain, the transparency of the water body can be gradually improved.

The invention relates to a method of cultivating biological flocs and an application thereof in breeding litopenaeus vannamei and relates to cultivation of biological flocs. The cultivated biological flocs comprise bacillus pumilus, lactobacillus acidophilus and pichia guilliermondii. The method comprises the following steps: preparing a bacillus pumilus inoculant, a lactobacillus acidophilus inoculant and pichia guilliermondii powder; adopting a way of splashing bacillus pumilus powder, saccharomycetes powder and a lactic acid bacteria inoculant for a water body per mu every day, and arranging an nano aerating tube in the lower layer around a cultivation pond to aerate to form the flocs; adjusting the C/N weight of a cultivating water body and controlling the C/N at (12-14): 1; and after the biological flocs are cultivated, splashing the bacillus pumilus inoculant, pichia guilliermondii powder and a lactobacillus acidophilus inoculant to the water body per mu every day. The cultivated biological flocs can be applied to breeding litopenaeus vannamei.

The invention relates to a preparation method of an elemental selenium-enriched preparation. The preparation method comprises the following steps: mixing sodium selenite, glucose, starch, protein, calcium carbonate, table salt, amino acids, potassium nitrate, ferrous sulphate, biochemical enzymes and chitosan with deionized water, then inoculating probiotics and fermenting at 25-35 DEG C for 20-40 days under an anaerobic condition to obtain the elemental selenium-enriched preparation, wherein the elemental selenium-enriched preparation contains 0.3-0.4g/L of elemental selenium, the elemental selenium is nanoscale, the average particle size is 60-150nm, and the total number of active bacteria is not less than 10 billions/mL; the probiotics are selected from one or the combination of more of saccharomycetes, lactic acid bacteria, bacillus and actinomycetes; the elemental selenium-enriched preparation contains nitrogen, phosphorus, potassium and trace elements necessary to plants except for selenium element, and has a production-increasing effect on grape; the selenium-enriched preparation has a stress-resistant effect on crop diseases and pests, has an antagonistic effect on harmful heavy metals, has a degradation effect on nitrate in vegetables, and can be used for reducing the application amounts of pesticides.

The invention relates to the field of microbial preparations and particularly discloses a compound microorganism bacterium agent and a preparation method and application thereof. The compound microorganism bacterium agent comprises lactic acid enterococcus faecalis, bacillus subtilis and bacillus megaterium, wherein the total viable count of the agent is not less than 3.5*108 cfu/mL. The compound microorganism bacterium agent is composed of reasonable strains according to composition characteristics of kitchen filtrate, can quickly degrade harmful substances in the kitchen filtrate, has a remarkable effect on degrading COD, BOD, ammonia nitrogen and the like in the kitchen filtrate and can effectively degrade organic matter to greatly relieve odor of the kitchen filtrate; the processed kitchen filtrate can also be used as a raw material of compound microorganism water-soluble fertilizer, so that the utilization rate of energy sources is increased, and environmental pollution caused by direct discharge of the kitchen filtrate is reduced.

The invention discloses bacillus coagulans BC-N6242 with collection number CCTCC (China Center for Type Culture Collection) M2012156. According to the invention, bacillus coagulans is mutated by low-energy nitrogen ion injection; and the screening of a high-glucose plate, a succinic acid plate and a pure-lactic acid plate and the lactobacillus casei'mixed fermentation' screening are adopted until the target strain is screened finally. The strain and the lactobacillus casei LC-N235 are proportionally mixed for fermentation. The seed culture solution is added to a 5L fermentation tank at a proportion of 2:1, and then fermentation and culture are performed for 48 hours; the output of the product L-lactic acid reaches 235g/L, which is improved by 45.1%, 57.7% and 14.6% as compared with that of separate fermentation of lactobacillus casei LC-N235, bacillus coagulans BC-3 and bacillus coagulans BC-N6242 respectively; the separate fermentation time of the bacillus coagulans is effectively shortened by 24 hours; and the bacillus coagulans and the application thereof in mixed fermentation to produce L-lactic acid provided by the invention have tremendous social significance and economic values.

The invention provides a microorganism bacterium agent for subtracting organic sludge. The microorganism bacterium agent is characterized in that: the microorganism bacterium agent per milliliter contains more than 2.7*10<9> of live bacteria, and the pH value is 3-4; the microorganism bacterium agent comprises photosynthetic bacteria, bacillus mucilaginosus, lactic acid bacteria, fusarium, anaerobic clostridium, denitrifying bacteria, sphaerotilus and zymogenous filamentous fungus. The invention also provides a corresponding production method of the microorganism bacterium agent and a complex culture medium. According to the invention, a mode of combining a plurality of floras is adopted to reduce and degrade the organic sludge and organic pollutants in water in a river (lake) or a municipal sewage treatment plant, and a limit that the effect is reduced due to single strain variation easily caused by environment change is avoided.

The invention relates to a preparation method of a microbial fertilizer. The method comprises the following steps of: separately fermenting bacillus, lactic acid bacteria, microzyme and photosynthetic bacteria to obtain mature fermentation liquids of different strains respectively; and mixing the mature fermentation liquids under a sterile condition to obtain the microbial fertilizer. The invention further relates to a microbial fertilizer prepared by using the microbial preparation method and application of the fertilizer. Due to the adoption of the microbial fertilizer, the problems of soil hardening, reduction in the fertility and the like caused by the adoption of a petrifaction fertilizer are solved.

The invention discloses a mutant of nitrile hydratase derived from caldalkalibacillus thermarum, and belongs to the technical field of enzyme engineering. The half-life period of the nitrile hydratasemutant Cal.t Nhase-A20V at 70 DEG C is about 10 min, the thermal stability of the mutant Cal.t Nhase-A20V has no too large change compared with that of a wild enzyme, and the specific enzyme activityof the mutant Cal.t Nhase-A20V is 128% of that of the wild enzyme. At the same time, the mutant also has better substrate tolerance and product tolerance, the final yield of nicotinamide produced bywhole-cell catalysis reaches 598 g/L; therefore, the nitrile hydratase mutant Cal.t Nhase-A20V provided by the invention has very good enzymatic properties, and is beneficial to industrial productionin future.

The invention provides Bacillus coagulans BC-TJ or its passage product Bacillus. The Bacillus coagulans BC-TJ has a preservation number of CGMCC NO. 14044. The Bacillus coagulans BC-TJ is a facultative anaerobic strain, can grow under aerobic and anaerobic conditions, has a high fermentation temperature, reduces contaminative microbe pollution probability during the fermentation process, is free of sterilization of the equipment, realizes open fermentation and reduces energy and the labor cost. The Bacillus coagulans BC-TJ utilizes lignocellulose hydrolyzate as a raw material to produce L-lactic acid, realizes a yield of 160+/-1.5 g/l and optical purity of 100% or more and has a fermentation production capacity of 2.2 g/l/h. In the fermentation process, reductive sugar supply is avoided and a production cost is further reduced. The Bacillus coagulans BC-TJ has the great potential in large-scale industrial production.