184 results about "Sporolactobacillus" patented technology

The invention discloses a culture method of anaerobic ammonia oxidation granular sludge with a polydopamine modified charcoal-loaded Fe-Cu sodium alginate microsphere kernel, which comprises the following steps: modifying charcoal by using polydopamine, then carrying out Fe-Cu ion loading, finally preparing sodium alginate microspheres, and taking the prepared sodium alginate microspheres as the kernel to prepare the anaerobic ammonia oxidation granular sludge with the polydopamine modified charcoal-loaded Fe-Cu sodium alginate microsphere kernel. And co-culturing the sodium alginate microspheres and activated bacillus DMF-4 in an oxygen-free environment, continuously culturing for 3 weeks, and collecting by using a gravity settling or centrifugal method to obtain the anaerobic ammonium oxidation granular sludge with the sodium alginate microsphere core. The sodium alginate microspheres serving as the core provide large density and good morphological support for sludge particles, the problem of sludge bulking is avoided, and the settling performance is improved. According to the present invention, the sludge anti-shearing performance is improved, the adsorption capacity of the slowly released and continuously slowly released iron ions on organic matters, ammonia nitrogen and heavy metals is enhanced, the sewage treatment effect is improved, and the iron ions are the biodegradable material so as not to cause secondary pollution in the use process.

The invention relates to the technical field of animal feeds, and provides an animal feed and a preparation method and optimization method thereof, and the optimization method of the animal feed comprises the following steps: determining candidate microorganisms and nutrition raw material formulas, establishing an optimization model, and solving to obtain an optimal microorganism formula and an optimal nutrition raw material formula; the animal feed comprises microorganisms such as lactic acid bacteria, saccharomycetes and bacillus and nutritional raw materials such as corn and soybean meal. By adopting a multilayer microcapsule technology, the survival rate of microorganisms in an acid environment is improved, the digestion and absorption efficiency is improved, and the nutritional condition and the milk production performance are improved; the invention further provides a corresponding preparation method, and the feed has the remarkable slow release characteristic and the effect of improving the feed utilization rate.

The invention discloses a preparation technology of directional debitterizing yellow tea, and belongs to the technical field of tea processing. Fresh tea leaves are used as raw materials, the yellow tea is prepared through the processes of pretreatment, fermentation, fixation, rolling, heaping for yellowing and drying, during fermentation, a compound bacteria solution and the spread and aired fresh leaves are mixed and fermented, strains in the compound bacteria solution comprise lactic acid bacteria, saccharomycetes and bacillus, and through the synergistic effect of the lactic acid bacteria, the saccharomycetes and the bacillus, the yellow tea is prepared. Balancing of directional degradation of bitter and astringent substances and generation of flavor substances is achieved, high-quality yellow tea is prepared through efficient debitterizing, the stage heaping yellowing technology is adopted, primary heaping and secondary heaping are adopted for two-stage heaping yellowing, the quality of the yellow tea is enhanced, and poor taste caused by excessive heaping yellowing is avoided. According to the finished tea prepared by the preparation process, the content of tea polyphenol is reduced to 18%-20% through detection, the content of amino acid reaches 4.5%-5.0%, bitter taste and astringent taste are relieved, sweet taste is added, and the taste is mellow.

The invention relates to the technical field of agricultural waste recycling, and discloses a formula and a preparation method of an amaranthus hypochondriacus and moso bamboo powder microbial fermentation feed, and the materials comprise amaranthus hypochondriacus straw, moso bamboo powder, corn flour, a mineral premix, salt and a microbial fermentation agent. After fermentation, the content of crude fibers is obviously reduced, the content of crude proteins is increased, the effect is optimal according to the proportion of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5), lignin in the moso bamboo powder is partially degraded, polysaccharide substances are released, the palatability of the feed is improved, the pH value of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5) in the optimal fermentation group is reduced to 4.2, the content of lactic acid reaches 2.3 g / kg, the total amount of VFA is increased, and the reproduction of harmful bacteria is inhibited; the reasonable proportion of the amaranthus hypochondriacus and the moso bamboo powder can balance carbon and nitrogen sources and promote synergistic fermentation of microorganisms, degradation of crude fibers in the fermentation process is related to acid production of lactic acid bacteria and cellulase secretion of bacillus, and the fermented feed can be used as a coarse feed substitute for ruminants or used in pig feed to improve intestinal health.

The invention provides a banana scab control method, and relates to the technical field of banana planting. The prevention and treatment method mainly comprises the following steps: preparing an antibacterial agent from an alocasia macrorrhiza fermentation extract, an okra low-temperature extract, peppermint essential oil, ethanol and the like, and spraying the antibacterial agent to banana plants, wherein the alocasia macrorrhiza fermentation extract is prepared by preparing alocasia macrorrhiza into slurry, mixing the slurry with bacillus subtilis and lactic acid bacteria, fermenting, boiling, sterilizing and carrying out ultrasonic alcohol extraction. The low-temperature okra extract is prepared by mixing fresh okra with ice water, crushing, performing ultrasonic extraction and freeze-drying. According to the invention, the defects in the prior art are overcome, the banana scab is prevented and controlled by taking plant extracted components as main functional substances, the environmental pollution caused by chemical drugs is reduced on the basis of effectively playing a prevention and control effect, and the safety of banana planting is comprehensively improved.

The invention discloses a high-yield protease salt denitration mycobacillus for improving the quality of low-salt shrimp paste, and belongs to the field of food biotechnology and application. The salt denitration mycobacillus XJ-229 provided by the invention has relatively good salt tolerance (the tolerance range is 1%-22%) and enzyme production (the protease production is 15.38 U / mL) capacity, and a proper fermentation agent is provided for fermentation of low-salt shrimp paste. The cladobacillus sp. XJ-229 is used for preparing shrimp paste, the content of volatile basic nitrogen, malondialdehyde and biogenic amine can be reduced, the content of free amino acid can be increased, the flavor of the shrimp paste can be improved, and the prepared shrimp paste can be accepted by people more easily. Therefore, the salt denitration mycobacillus XJ-229 provided by the invention is beneficial to improving the quality of the shrimp paste and improving the market acceptability of the shrimp paste.

The invention belongs to the technical field of medicines, and particularly relates to a rhizoma polygonati stem and leaf fermented feed additive and application thereof. The compound probiotics of bacillus, saccharomycetes and lactic acid bacteria are used as a feed additive, the additive for the rhizoma polygonati stem and leaf feed is provided and applied to preparation of the rhizoma polygonati stem and leaf fermented feed, the fermented feed prepared by the additive remarkably improves the dissolution of the content of total phenols and polysaccharides in the rhizoma polygonati stem and leaf feed, and the content of the total phenols and polysaccharides in the rhizoma polygonati stem and leaf feed is increased. The palatability of the feed is improved, the immunity of animals is favorably enhanced, the health and production performance of the animals are improved, the development and industrial application of medicinal and edible medicinal materials in the animal feed are promoted, and the feed has a wide prospect.

The invention discloses a mulberry leaf-vinasse fermentation composition which comprises the following raw materials: vinasse, mulberry leaves and a complex microbial inoculant, the complex microbial inoculants comprise saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum. The mulberry leaf-vinasse fermentation composition is obtained by performing anaerobic solid state fermentation on the raw materials, namely crushing vinasse and mulberry leaves to 20-50 meshes, uniformly mixing, adjusting the water content of the raw materials to be 50-70wt%, inoculating a complex microbial inoculant, uniformly stirring, filling into a breathing bag, sealing at 37 + / -0.5 DEG C, and fermenting for 4-6 days, thereby obtaining the mulberry leaf-vinasse fermentation composition. According to the mulberry leaf-vinasse fermentation composition, the mulberry leaves and the vinasse are mixed, then the complex microbial inoculant is inoculated for solid-state fermentation, the pH value of the prepared mulberry leaf-vinasse fermentation composition is remarkably reduced, meanwhile, the lactic acid content is remarkably increased, the antinutritional factor content is reduced, true protein is well preserved, the antioxidant activity is more reserved, and the fermentation effect of the mulberry leaves and the vinasse is improved; the feeding value of the mulberry leaves and the vinasse is effectively improved.

The invention discloses a method for preparing a high-carbon-nitrogen-ratio organic carbon source and a high-protein feed from kitchen waste solid residues. According to the method, kitchen waste solid residues are sequentially subjected to anaerobic fermentation and drying treatment to obtain dry powder and water vapor, the dry powder serves as feed, the water vapor is subjected to condensation treatment to obtain condensed water, the condensed water is subjected to membrane concentration treatment to obtain a concentrated solution, and the concentrated solution serves as an organic carbon source; strains adopted in the anaerobic fermentation process comprise at least one of saccharomycetes, lactic acid bacteria, bacillus aceticus and clostridium. According to the method, the kitchen waste solid residues can be converted into high-quality feed raw materials and organic carbon sources, high-quality utilization of the kitchen waste is achieved, the economic value of the waste is improved, meanwhile, the kitchen waste treatment process is optimized, and environmental pollution is reduced.

The invention discloses a special organic fertilizer for kiwi fruits and a preparation method thereof. The organic fertilizer comprises the following raw materials in parts by weight: 50-70 parts of livestock and poultry manure, 30-50 parts of kiwi fruit branch and leaf residues, 10-20 parts of soybean meal, 5-10 parts of bone meal, 3-8 parts of humic acid and 1-3 parts of a microbial agent. The microbial agent comprises bacillus subtilis, bacillus mucilaginosus, saccharomycetes and lactic acid bacteria in a ratio of 1: 1: 1: 1. The raw materials are pretreated and then subjected to a mixed fermentation process to prepare the finished product. The organic fertilizer is comprehensive in nutrition, can meet the nutritional requirements of kiwi fruit growth, improves the soil environment, improves the fruit quality and yield, and has remarkable economic benefits and environmental benefits.

The invention provides a preparation method of probiotic fermented polygonatum sibiricum polysaccharide, and belongs to the field of traditional Chinese medicinal material processing. The preparation method comprises the following steps: (1) adding cellulase and pectinase into polygonatum kingianum, carrying out water extraction, carrying out enzyme deactivation after water extraction, and carrying out suction filtration to obtain filtrate; (2) preparing bacillus DU-106 and lactobacillus plantarum nbk-MA2 into bacterial powder, and adding water to obtain bacterial liquid; mixing the bacterial liquid with the filtrate, fermenting, performing suction filtration, and centrifuging to obtain supernate; performing alcohol precipitation to remove protein; carrying out ethanol precipitation by using absolute ethyl alcohol again, collecting the precipitate after centrifuging, and carrying out redissolution, dialysis and freeze-drying to obtain fermentation modified crude polysaccharide; and (3) purifying the fermented and modified crude polysaccharide to obtain a purified polysaccharide component. The obtained probiotic fermented polygonatum sibiricum polysaccharide has the effect of losing weight, and can obviously improve the obesity characteristics of mice, specifically, reducing body weight, reducing fat, reducing serum liver lipid level, relieving serum liver inflammation of the mice, weakening blood glucose insulin sensitivity, repairing liver injury and the like.

The present invention discloses a process for preparing lactic acid by mechanically enhanced ammonia method from straw-like biomass. The enzymatic hydrolysate of solid fibers obtained by the mechanically enhanced ammonia method from straw-like biomass is used as the carbon source of the fermentation medium in the process of fermenting lactic acid. The preparation of the carbon source in the fermentation medium comprises the following steps: S1: treating straw-like lignocellulosic biomass with a pretreatment liquid containing ammonia water and inorganic ammonium salts in a twin-screw device; S2: subjecting the obtained material to a heat preservation reaction, and after solid-liquid separation, obtaining solid fibers containing holocellulose; S3: enzymatically hydrolyzing the obtained solid fibers with Trichoderma reesei cellulase and xylanase, and performing solid-liquid separation, and the obtained supernatant is used as the carbon source in the fermentation medium for fermenting L-lactic acid. The present invention uses the sugar liquid obtained by enzymatic hydrolysis and saccharification of pretreated holocellulose, inoculates Bacillus coagulans to ferment L-lactic acid after supplementing nitrogen source and inorganic salts, and can greatly improve the concentration and conversion rate of L-lactic acid.

The invention discloses a fermented feed prepared from traditional Chinese medicine residues as well as a preparation method and application of the fermented feed. The fermented feed is prepared by mixing and fermenting a mixed bacteria solution formed by fermenting bacillus subtilis, bacillus coagulans, phaffia rhodozyma and lactobacillus plantarum with bran, soybean meal, rice husks, zeolite powder and honeysuckle dregs. The fermented feed disclosed by the invention increases the contents of microbial protein, lactic acid, ethanol, tartaric acid, acetic acid and lactic acid bacteria, contains various fermentation substances, can improve the appetite of animals and improve the palatability, and is high in nutritional value. Honeysuckle herb residues are used as main raw materials, so that the preparation method has the characteristic of low production cost, and the problem of environmental pollution caused by random discarding of herb residue wastes is solved.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention relates to a microbial water body substrate modifier and application thereof. The microbial water body substrate modifier comprises the following components: compound microbial powder, zeolite powder, shell powder and polyferric sulfate, the compound microbial powder comprises bacillus powder and lactic acid bacteria powder. The microbial water substrate modifier can effectively realize rapid bottom modification of aquaculture water, improve water quality, optimize aquaculture environment, reduce diseases and promote healthy growth of aquaculture animals. Wherein the bacillus can flocculate and settle residual organic matters such as feed, animal waste and the like in the culture water body by utilizing the flocculation effect of the bacillus, and the physical adsorption components, namely the zeolite powder and the shell powder, and the chemical adsorption component, namely the polyferric sulfate are synergistically matched, so that suspended organic matter large particles in the water body are effectively adsorbed and settled; and the organic matters are jointly degraded through the common graded degradation effect of the bacillus and the lactic acid bacteria, so that the effects of improving the substrate of the aquaculture water body and promoting the healthy growth of the aquaculture animals are achieved.

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention relates to the technical field of gene engineering, in particular to a D-psicose-3-epimerase mutant and application of the D-psicose-3-epimerase mutant in synthesis of psicose. According to the present invention, the 59th site, the 106th site and the 188th site of the wild type D-psicose-3-epimerase derived from Brevibacillus thermophilus (Brevibacillus thermophilus) are subjected to simultaneous mutation to obtain the D-psicose-3-epimerase mutant, such that the efficiency of producing D-psicose by using fructose as the raw material is significantly improved, the reaction of catalyzing 500 g / L fructose to produce D-psicose is performed for 4 h, and the yield of the D-psicose is significantly improved; the conversion rate is up to 36.5%; according to the present invention, the coexpression binding enzyme strain is constructed by coupling glucose isomerase, such that the intermediate transfer efficiency and the enzyme stability are improved, the reaction for producing D-psicose through catalysis of 500 g / L glucose is performed for 6 h, and the conversion rate can achieve 30.5%; and the activity of the immobilized enzyme can still be kept at 80% after the immobilized enzyme is repeatedly used for 10 batches.

The invention relates to the technical field of fermentation, in particular to a bean sour soup processing method based on bean clear liquid fermentation, and the processing method comprises the following steps: S1, filtering bean clear liquid, adjusting dry matter concentration, adding glucose, pasteurizing, and cooling; s2, inoculating a mixed strain of aspergillus oryzae and aspergillus sojae, and performing aerated fermentation; s3, inoculating a lactic acid bacteria mixed strain, and carrying out sealed anaerobic fermentation; s4, carrying out hot filtration, centrifugation and ultra-high temperature sterilization on the fermentation liquid, inoculating bacillus velezensis and gluconacetobacter, carrying out aeration stirring fermentation, inoculating saccharomycetes, and standing for anaerobic fermentation; and S5, carrying out solid-liquid separation on the fermentation liquor, and sterilizing to obtain the bean sour soup product. According to the method, multi-stage ordered fermentation is combined with a multi-strain synergistic effect, and parameters such as filtration, inoculation amount and temperature in each stage are optimized, so that efficient debitterizing, umami enhancement, aroma level enrichment and sour taste softening are realized, sanitation, safety and quality stability of products are guaranteed, and the taste and quality of the bean sour soup are effectively improved.

The invention discloses a high-yield caproic acid bacterium and application thereof in production of caproic acid by anaerobic fermentation of white spirit brewing yellow water. According to the invention, a high-yield caproic acid bacterium, namely bacillus aryabhattai H1 (CCTCC NO: M 20241040), is obtained through separation and screening, the high-yield caproic acid bacterium can grow and synthesize caproic acid by independently taking ethanol or lactic acid as a substrate, and the caproic acid production efficiency is optimal when ethanol and lactic acid are taken as a composite substrate. According to the bacillus aryabhattai H1, under the open type fermentation condition that a substrate does not need to be sterilized, the caproic acid can be efficiently produced by taking the white spirit brewing byproduct yellow water as the substrate, and the yield, the yield and the production intensity of the caproic acid can reach 12.5 g / L, 2.5 kg / m < 3 > / d and 22% or above respectively.

The invention relates to a method for preparing fish feed by secondary utilization of traditional Chinese medicine wastes, and belongs to the technical field of fish feed. After the traditional Chinese medicine waste is pretreated to remove heavy metal, primary fermentation of bacillus subtilis and aspergillus niger is carried out, the bacillus subtilis can degrade cellulose, the aspergillus niger decomposes antinutritional factors, then fermentation of lactobacillus lactis and candida utilis is carried out, lactic acid bacteria produce acid to inhibit infectious microbes, flavor substances are synthesized, and palatability is improved. Through traditional Chinese medicine residue pretreatment, multi-strain synergistic fermentation and a scientific feed formula, efficient reutilization of traditional Chinese medicine waste is successfully realized, and the fish feed which is rich in nutrition, high in safety and remarkable in economic benefit is produced.

The invention belongs to the technical field of food processing, and particularly relates to an extraction process of turnip seed oil and an effective component composition of the turnip seed oil. The invention relates to an extraction process of turnip seed oil, which comprises the following steps: performing staged fermentation pretreatment on turnip seed broken cell walls by using a plurality of microbial strains, and then extracting grease by combining with a nano SiO2 (at) SDS composite active agent and ionic liquid synergistic ultrasonic treatment. Through staged fermentation pretreatment and the synergistic effect of the nano material and the ionic liquid, the extraction efficiency and quality of the turnip seed oil are greatly improved; firstly, cell walls and internal components are gradually degraded by utilizing aspergillus niger, aspergillus oryzae, bacillus licheniformis, lactic acid bacteria and saccharomycetes, grease is released, and the stability of the grease is improved; then, the extraction process is further optimized by a composite active agent formed by nano silicon dioxide and lauryl sodium sulfate and 1-butyl-3-methylimidazolium acetate, and the efficiency is enhanced through ultrasonic treatment;

The present invention discloses an application of a moisturizing coating with a flavoring function on cigarettes. The moisturizing coating comprises: a composite bacterial solution, a tobacco polysaccharide solution, and a chitosan solution. The volume ratio of the composite bacterial solution, the tobacco polysaccharide solution, and the chitosan solution is (3-4): (2-3): (4-5). The composite bacterial solution is formed by mixing lactic acid streptococci, bacillus, and yeast in a certain proportion. In the moisturizing coating of the present application, the secondary metabolites of lactic acid streptococci can inhibit miscellaneous bacteria, chitosan is a natural and harmless antibacterial film, and tobacco polysaccharides themselves have moisturizing properties. The composite bacterial solution can interact with tobacco polysaccharides and chitosan to better inhibit miscellaneous bacteria, and the protective film has a better protective effect. The metabolites of bacillus and yeast produce aroma-causing substances during the combustion and cracking process of cigarettes, thereby increasing the aroma of cigarettes.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The invention provides an aquatic product fermented feed containing probiotics. The fermented feed is prepared from the following raw materials: fish meal, soybean meal, peanut meal, flour, bone meal, fish soluble, soybean oil, phospholipid and the probiotics. According to the invention, the breathing valve fermentation bag is used, so that anaerobic bacteria and probiotics which play a role are cultivated and fermented under an anaerobic or semi-anaerobic condition. Meanwhile, the breeding and growth of aerobic harmful bacteria can be inhibited through anaerobic culture, and the growth of beneficial bacteria such as bacillus, lactic acid bacteria and clostridium butyricum is facilitated; the compounded probiotic group is screened, acid can be stably produced within a short time, the acid production content is high, and after a period of culture, the biological growth curve of the screened probiotic group can reach a balanced state within about 2 days; the compounded probiotic group can generate butyric acid, lactic acid and other substances through metabolism, so that the PH of the digestive tract of the fish body is reduced, the reproduction of beneficial bacteria is promoted, and the growth of harmful bacteria is inhibited.

The invention discloses a compound microbial agent and application thereof, and belongs to the technical field of microbial agents. The method specifically comprises the following steps: adding clostridium butyricum fermentation centrifugate into a fermentation tank, adding a nitrogen source, a carbon source and inorganic salt into the fermentation tank, then adding a bacillus subtilis solution and a bacillus licheniformis solution, stirring at a certain temperature and a certain rotating speed, fermenting for a period of time, then adding a lactic acid bacteria solution, and fermenting for a period of time at a certain rotating speed; stirring and fermenting at a certain rotating speed at a certain temperature, detecting the pH value after fermenting for a period of time, and when the pH value is reduced to 4.3-4.5, obtaining the compound microbial agent. The prepared compound microbial agent can improve the saline-alkali soil, effectively improve the pH of the saline-alkali soil, consume soil salinity and improve the crop germination rate.

The application discloses a Qingjiang Aurantii Fructus extract with soothing and repairing effects on atopic dermatitis and application thereof, and a preparation method of the Qingjiang Aurantii Fructus extract comprises the following steps: A1, crushing Qingjiang Aurantii Fructus, adding water to soak, and then sterilizing; A2, inoculating probiotics in the solution after sterilization in step A1 to perform fermentation; A3, filtering the obtained fermentation liquor after sterilization, and purifying to obtain the Qingjiang Aurantii Fructus extract; wherein the probiotics are at least one selected from lactic acid bacteria, bacillus and yeast fungi. The Qingjiang Aurantii Fructus extract obtained by fermenting Qingjiang Aurantii Fructus with specific probiotics has significant soothing, repairing and anti-inflammatory effects, and has the soothing and repairing effects on atopic dermatitis in particular.

The invention relates to the technical field of fertilizers, in particular to a microbial amino acid-containing water-soluble fertilizer and a preparation method thereof. The method comprises the following steps: providing a first corn steep liquor and a second corn steep liquor; adding a Trichoderma harzianum-Purpureocillium lilacinum complex microbial inoculant and lactic acid bacteria into the first corn steep liquor, and carrying out first fermentation to obtain a first fermentation broth; mixing the first fermentation liquor with the second corn steep liquor to obtain mixed corn steep liquor; adding bacillus subtilis into the mixed corn steep liquor, and carrying out second fermentation to obtain second fermentation liquor; and sequentially adding a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer into the second fermentation liquor to obtain the water-soluble fertilizer containing microbial amino acids. The microbial amino acid-containing water-soluble fertilizer prepared by the preparation method provided by the invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility, promote the root development of the crops and improve the resistance of the crops to adversity.

The invention discloses a compound microorganism fermented attenuated aroma-enhanced potato residue feed and a preparation method thereof, and belongs to the field of feed processing. The feed comprises potato residues, crop straw particles, corn flour, bran, soybean meal, urea, ammonium sulfate and a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis and lactobacillus plantarum. The preparation method comprises raw material pretreatment; preparing a microbial agent; performing mixed fermentation; and post-processing. The bacillus velezensis secretes glycosidase, and glucosidic bonds of the glycoalkaloid are subjected to enzymolysis; lactobacillus plantarum is metabolized to generate lactic acid to create an acidic environment to promote chemical degradation of glycoalkaloid, and the double bacteria cooperate to inhibit growth of mould, so that efficient attenuation is realized. The bacillus velezensis metabolizes to generate glucose, ethanol and free amino acid, and the lactobacillus plantarum metabolizes to generate ester flavor substances, so that the feed is endowed with strong fermentation aroma. Meanwhile, the pungent smell of volatile aldehyde substances is eliminated through double-bacterium synergism.

The invention discloses an artificial bait additive for improving intestinal flora of yellow fin tuna, which comprises 15-30% of fish meal, 15-30% of soybean meal, 5-10% of wheat starch, 2-5% of fish oil, 1-3% of rapeseed oil, 5-10% of mulberry leaf powder, 5-15% of fly maggot powder, 1-5% of microalgae, 0.1-0.5% of bile acid, 1-3% of pine needle meal, 5-15% of brown rice and 1-5% of cow milk casein. The Chinese herbal medicine active components comprise 0.01%-0.1% of an acanthopanax senticosus extract, 0.05%-0.5% of ophiopogon japonicus polysaccharide, 1%-5% of bitter gourd dry powder, 0.01%-0.1% of a platycodin extract and 1%-5% of a fresh peach leaf fermentation product; the probiotic complexing agent comprises the following components in percentage by weight: 0.01%-0.5% of bacillus subtilis fermentation liquor, 0.01%-0.2% of lactobacillus acidophilus freeze-dried powder and 0.01%-0.3% of bacillus licheniformis fermentation liquor, and the auxiliary components comprise 1%-5% of a brewer's grain fermentation substrate, 0.1%-0.5% of a fish oil emulsifier and 0.1%-1% of fructo-oligosaccharide. According to the present invention, the growth performance of the yellow-fin tuna can be comprehensively promoted, the immunity is enhanced, the intestinal health of the yellow-fin tuna is optimized, and the feed conversion rate can be increased.