18 results about "Sporolactobacillus" patented technology

The application discloses a Qingjiang Aurantii Fructus extract with soothing and repairing effects on atopic dermatitis and application thereof, and a preparation method of the Qingjiang Aurantii Fructus extract comprises the following steps: A1, crushing Qingjiang Aurantii Fructus, adding water to soak, and then sterilizing; A2, inoculating probiotics in the solution after sterilization in step A1 to perform fermentation; A3, filtering the obtained fermentation liquor after sterilization, and purifying to obtain the Qingjiang Aurantii Fructus extract; wherein the probiotics are at least one selected from lactic acid bacteria, bacillus and yeast fungi. The Qingjiang Aurantii Fructus extract obtained by fermenting Qingjiang Aurantii Fructus with specific probiotics has significant soothing, repairing and anti-inflammatory effects, and has the soothing and repairing effects on atopic dermatitis in particular.

The application discloses an acidification soil heavy metal passivation and acidification treatment integrated method, and aims to solve the pollution problem of karst pyrite mine area soil. The method comprises the following steps: S1, preparing an improving agent: S11, taking modified dolomite powder, modified rectorite, sulfonated asphalt powder-pullulan composite microspheres, modified chitosan quaternary ammonium salt, modified fly ash based pectin carrier and modified rice husk ash, and uniformly mixing the same to obtain a mixture; S12, adding hydroxyapatite-zeolite composite filler into the mixture, then adding water to adjust the water content of the system to 25%-40%, inoculating a jelly-like bacillus-paenibacillus complex inoculant, fully stirring and mixing, and preparing the improving agent. The method can effectively optimize soil structure, stabilize lead, zinc, cadmium, arsenic and other heavy metals, degrade sulfates, improve soil fertility and microbial activity, and is suitable for the environmental characteristics of karst area rainfall leaching, and can meet the treatment and application requirements of the compound contaminated soil.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

This invention relates to the field of bio-infectious agent metabolites, and discloses a bio-infectious agent metabolite for the safe improvement of agricultural products and its preparation method. The bio-infectious agent metabolite is obtained by fermenting a fermentation raw material composed of a carbon source and a nitrogen source with an immobilized composite microbial agent on an organic-inorganic composite carrier. The organic-inorganic composite carrier immobilized composite microbial agent is prepared by immobilizing the composite microbial agent onto a chitosan-sodium alginate organic embedding carrier and an inorganic adsorbent carrier using immobilization loading technology. The composite microbial agent consists of *Bacillus circinus*, *Bacillus licheniformis*, *Bacillus amyloliquefaciens*, and *Bacillus subtilis*. This invention provides a method for preparing bio-infectious agent metabolites, and yields a bio-infectious agent metabolite containing carbohydrate compounds, amino acid compounds, organic acid compounds, and plant hormones, which has a quality-improving effect on agricultural products.

This invention discloses a salt-tolerant, alkali-tolerant, and hydrocarbon-loving composite functional microbial agent for the remediation of oil-containing saline-alkali land, its preparation method, and its application. It relates to the field of environmental microbiology and soil bioremediation technology. The composite microbial agent is composed of halophilic Bacillus, alkaliphilic Pseudomonas, and petroleum hydrocarbon-degrading Mycobacterium, compounded in a viable count ratio of 3:2:2, with a single strain having a viable count ≥ 5.0 × 10⁻⁶. 9 This invention, with a concentration of CFU / g, can tolerate extreme environments including 12% NaCl concentration and pH 9.5. Through targeted screening and precise formulation of functional bacterial strains, it achieves a synergistic effect of salt and alkali tolerance and broad-spectrum petroleum hydrocarbon degradation. It can be widely applied in oilfield ecological restoration projects, recultivation of saline-alkali polluted farmland, and in-situ bioremediation of oil- and salt-alkali co-contaminated soils. The preparation process is simple, and the bacterial cell activity retention rate is high, solving the technical problems of poor stress resistance and low degradation efficiency of traditional degrading agents. It possesses excellent remediation effects and industrial application value.

The application belongs to the technical field of bioactive substance preparation and agricultural application, and discloses a beta-amino butyric acid, a whole cell conversion preparation method thereof and application thereof. The method uses Paenibacillus halophilus as a catalyst to perform biological conversion in a reaction system containing a substrate, so as to obtain the beta-amino butyric acid. The method has mild reaction conditions, the bacterial cells can be repeatedly used, the conversion rate is high, and the green, efficient and low-cost production of the beta-amino butyric acid is realized. The application also provides application of the beta-amino butyric acid prepared by the above method in improving salt stress resistance of crops and in plant disease control as a pesticide synergist. Experiments show that the beta-amino butyric acid can significantly alleviate the inhibition of salt stress on the growth of wheat, and improve the chlorophyll content and biomass accumulation; the beta-amino butyric acid has a synergistic effect on grape diseases when used in combination with anilazodifen and fluoroacetamide, and meanwhile, the fruit quality and storage resistance are improved.

The application discloses a method for producing feed by using Gramineae plant waste, and comprises the following steps: S1, raw material pretreatment: after the Gramineae plant waste is dedusted, cut or crushed, water immersion and ammoniation treatment are carried out, so that the water content reaches 30-60%, and the ammonia addition amount is 3-5% of the dry weight; S2, gas phase blasting: the pretreated material is subjected to gas phase blasting, and the medium is selected from water vapor or non-reactive compressed gas; S3, microbial agent fermentation: a compound microbial agent containing at least two of lactic acid bacteria, yeast bacteria, bacillus and cellulose-decomposing bacteria is added to the blasted material, the water content is adjusted to 60-70%, and sealed fermentation is carried out for 3-7 days; S4, granulation: the fermented material is dried to have a water content of 12-18%, other raw materials are mixed after crushing, and the feed product is obtained through steam conditioning and pressing granulation. Through the cooperation of multiple processes, the method can significantly destroy the structure of the straw, improve the crude protein content and the feeding value, and the product is convenient to store and transport.

The present invention relates to Lacticaseibacillus paracasei HP7 with preventive or alleviative efficacy for functional dyspepsia and uses thereof. The composition comprising the Lacticaseibacillus paracasei HP7 strain (accession number: KCTC 13143 BP) of the present invention as an active ingredient was confirmed to increase expression of intestinal tight junction genes, improve gastrointestinal peristalsis and gastric emptying effects, enhances gastric weight and gastric emptying levels associated with gastric emptying, and significantly increase blood hormone levels related to digestive activity, thereby improving dyspepsia.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

This invention discloses a microbial composition, a gel material, a preparation method, and its application in afforestation of saline-alkali land, belonging to the field of microbial technology. It is composed of *Bacillus curvaturetus* (…). Bacillus bent E1, Bacillus argentea ( Bacillus aryabhattai Composed of GPR018 and lactic acid bacteria; Bacillus curvatureis ( Bacillus flexus The accession number for E1 is CGMCC No. 31832. The surface charge of these three microorganisms can synergistically interact with the gel network of the gel material, effectively inhibiting the excessive adsorption of sodium ions from the soil by the polymer lattice of the gel material. This reduces the risk of secondary soil salinization caused by the gel material lattice's "only adsorption and no release" of sodium ions, fundamentally solving the problem of soil salt accumulation caused by the retention of sodium ions during the repeated water absorption and release process of traditional water-retaining agents.

The application belongs to the technical field of biological fermentation, and discloses a method for efficiently preparing earthworm functional liquid by using earthworm intestinal endogenous probiotics and enzymes and the earthworm functional liquid. The method comprises the following steps: step 1: activating and expanding B. velezensis XY1, P. megaspora and Lactococcus lactis respectively to obtain B. velezensis XY1 bacterial liquid, P. megaspora bacterial liquid and Lactococcus lactis bacterial liquid; and step 2: inoculating the B. velezensis XY1 bacterial liquid, the P. megaspora bacterial liquid, the Lactococcus lactis bacterial liquid and a compound enzyme into earthworm slurry to obtain the earthworm functional liquid. The B. velezensis XY1, the P. megaspora, the Lactococcus lactis and the compound enzyme are used for synergistic fermentation to obtain the earthworm functional liquid, the content of total amino acids and small peptides is significantly improved, and the effect of the earthworm functional liquid can be further improved.

The application discloses a kind of biological enzyme deodorization antibacterial agent for sewage treatment, including: biological complex enzyme preparation 10~30 parts by weight, probiotic preparation 5~15 parts by weight, carrier 40~70 parts by weight, auxiliary agent 1~8 parts by weight;Biological complex enzyme preparation includes at least three kinds of protease, lipase, cellulase, urease and sulfatase;Probiotic preparation includes at least one of bacillus, lactic acid bacteria, photosynthetic bacteria;Preparation method includes: carrier drying, crushing and sieving, obtain pretreated carrier;Biological complex enzyme preparation, probiotic preparation and auxiliary agent are uniformly mixed to obtain mixed powder;Mixed powder and pretreated carrier are stirred and mixed under normal temperature sterile condition to obtain primary product;Primary product is dried and sieved at low temperature to obtain biological enzyme deodorization antibacterial agent.The application has the advantages that: solve the secondary pollution, short-term effect, poor synergy and complex preparation of existing deodorization antibacterial agent, realize efficient deodorization, long-acting antibacterial and no secondary pollution, and the preparation process is simple, cost controllable.

This invention discloses a method and feed for preparing low-soybean meal compound fermented feed using peanut meal and apple pomace, belonging to the field of feed production technology. The method involves compounding apple pomace, peanut meal, wheat bran, and corn husk with water, first inoculating with lactic acid bacteria and yeast for acidification fermentation, then inoculating with Bacillus, Aspergillus, and / or compound enzymes for enzymatic fermentation, and finally processing the feed through drying, wet processing, or semi-drying. This method can increase the content of acid-soluble proteins and small peptides, improve palatability and storage stability, and reduce the amount of soybean meal used.

This invention discloses a fermentation process for Liushenqu (a traditional Chinese medicine fermentation product) and its application, specifically relating to the field of traditional Chinese medicine fermentation technology. The fermentation process involves pretreatment of raw materials, followed by inoculation of seed cultures of *Rhizopus oryzae*, *Bacillus subtilis*, and *Wickham's yeast* in a sterile substrate for aerobic fermentation in a specific ratio. Finally, after the environment has been acidified, seed cultures of *Lactobacillus plantarum* that have been induced are inoculated, and fermentation continues at a controlled temperature until the pH reaches 4.0-4.5. Fermentation is then stopped, yielding the fermented Liushenqu. This invention employs a method of rapid enzyme production and environmental acidification using molds and yeasts in the pre-fermentation stage, followed by deep transformation by lactic acid bacteria in the main fermentation stage. This approach simultaneously pursues high enzyme activity and high content of active ingredients, overcoming the limitations of existing technologies that focus only on a single indicator. Through the transformation effect of lactic acid bacteria, richer secondary metabolites are also produced, resulting in a more comprehensive spleen-strengthening and digestion-aiding effect.

This invention relates to the field of microbial fermentation feed technology, and discloses a zero-wastewater feed ingredient production method based on two-stage fermentation. The method includes inoculating *Bacillus bellis* into a liquid fermentation medium for liquid fermentation to obtain a liquid fermentation mixture; centrifuging all of the liquid fermentation mixture to obtain *Bacillus bellis* cells and collecting the supernatant containing the remaining *Bacillus bellis*; adding yeast and lactic acid bacteria to the collected supernatant, then mixing it with solid fermentation raw materials to form a solid-liquid mixture; adding purified water or solid fermentation raw materials to control the fermentation moisture content; and then fermenting the moisture-adjusted solid-liquid mixture under sealed conditions to obtain a pre-fermented mixture as the feed ingredient. This invention avoids wastewater generation during the production process and improves the digestive enzyme activity of the product.

This invention belongs to the field of veterinary biological agent technology, specifically relating to a bacterial-enzyme complex preparation for the prevention and treatment of transmissible gastroenteritis (TGEV) in pigs, its preparation method, and its application. The bacterial-enzyme complex preparation comprises microcapsule particles; the core material of each microcapsule particle includes a complex of probiotics and a synergistic enzyme system; the complex of probiotics includes *Lactobacillus rhamnosus*, *Bacillus coagulans*, *Pediococcus pentosus*, and *Kluyveromyces marsupialis*. In the bacterial-enzyme complex preparation for the prevention and treatment of TGEV provided by this invention, the complex probiotics and the synergistic enzyme system form a synergistic effect. Lactic acid secreted by *Lactobacillus rhamnosus* can lower the intestinal pH, disrupting the TGEV survival environment; antimicrobial peptides produced by *Bacillus coagulans* can directly cleave the viral capsid protein; lysozyme can degrade viral envelope polysaccharides; and neutral protease, β-glucanase, and xylanase can destroy the viral adsorption sites on the intestinal mucosa. This quadruple action significantly enhances the antiviral effect.

This invention discloses a high-purity, multifunctional probiotic powder and its preparation method. Based on the different characteristics of lactic acid bacteria, Bacillus subtilis, and actinomycetes, this invention employs different methods for their respective proliferation. This not only avoids potential mutual interference and contamination during their individual proliferation processes but also achieves high viable cell counts while maintaining high purity, and the high viable cell count is maintained even after mixing. This invention mixes the proliferated bacterial powders in a reasonable ratio, allowing lactic acid bacteria to be the dominant strain while fully utilizing the functional characteristics of each of the three bacteria: lactic acid bacteria are anaerobic, while Bacillus subtilis and actinomycetes are aerobic. The latter can absorb oxygen released by the former, ensuring that in the anaerobic environment of the human intestine, Bacillus subtilis and actinomycetes remain active rather than dormant, generating resistant endospores through synergistic action with lactic acid bacteria.