77 results about "Sporolactobacillus" patented technology

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention relates to the technical field of fermentation, in particular to a bean sour soup processing method based on bean clear liquid fermentation, and the processing method comprises the following steps: S1, filtering bean clear liquid, adjusting dry matter concentration, adding glucose, pasteurizing, and cooling; s2, inoculating a mixed strain of aspergillus oryzae and aspergillus sojae, and performing aerated fermentation; s3, inoculating a lactic acid bacteria mixed strain, and carrying out sealed anaerobic fermentation; s4, carrying out hot filtration, centrifugation and ultra-high temperature sterilization on the fermentation liquid, inoculating bacillus velezensis and gluconacetobacter, carrying out aeration stirring fermentation, inoculating saccharomycetes, and standing for anaerobic fermentation; and S5, carrying out solid-liquid separation on the fermentation liquor, and sterilizing to obtain the bean sour soup product. According to the method, multi-stage ordered fermentation is combined with a multi-strain synergistic effect, and parameters such as filtration, inoculation amount and temperature in each stage are optimized, so that efficient debitterizing, umami enhancement, aroma level enrichment and sour taste softening are realized, sanitation, safety and quality stability of products are guaranteed, and the taste and quality of the bean sour soup are effectively improved.

The invention discloses a high-yield caproic acid bacterium and application thereof in production of caproic acid by anaerobic fermentation of white spirit brewing yellow water. According to the invention, a high-yield caproic acid bacterium, namely bacillus aryabhattai H1 (CCTCC NO: M 20241040), is obtained through separation and screening, the high-yield caproic acid bacterium can grow and synthesize caproic acid by independently taking ethanol or lactic acid as a substrate, and the caproic acid production efficiency is optimal when ethanol and lactic acid are taken as a composite substrate. According to the bacillus aryabhattai H1, under the open type fermentation condition that a substrate does not need to be sterilized, the caproic acid can be efficiently produced by taking the white spirit brewing byproduct yellow water as the substrate, and the yield, the yield and the production intensity of the caproic acid can reach 12.5 g / L, 2.5 kg / m < 3 > / d and 22% or above respectively.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The application discloses a Qingjiang Aurantii Fructus extract with soothing and repairing effects on atopic dermatitis and application thereof, and a preparation method of the Qingjiang Aurantii Fructus extract comprises the following steps: A1, crushing Qingjiang Aurantii Fructus, adding water to soak, and then sterilizing; A2, inoculating probiotics in the solution after sterilization in step A1 to perform fermentation; A3, filtering the obtained fermentation liquor after sterilization, and purifying to obtain the Qingjiang Aurantii Fructus extract; wherein the probiotics are at least one selected from lactic acid bacteria, bacillus and yeast fungi. The Qingjiang Aurantii Fructus extract obtained by fermenting Qingjiang Aurantii Fructus with specific probiotics has significant soothing, repairing and anti-inflammatory effects, and has the soothing and repairing effects on atopic dermatitis in particular.

The invention discloses a compound microorganism fermented attenuated aroma-enhanced potato residue feed and a preparation method thereof, and belongs to the field of feed processing. The feed comprises potato residues, crop straw particles, corn flour, bran, soybean meal, urea, ammonium sulfate and a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis and lactobacillus plantarum. The preparation method comprises raw material pretreatment; preparing a microbial agent; performing mixed fermentation; and post-processing. The bacillus velezensis secretes glycosidase, and glucosidic bonds of the glycoalkaloid are subjected to enzymolysis; lactobacillus plantarum is metabolized to generate lactic acid to create an acidic environment to promote chemical degradation of glycoalkaloid, and the double bacteria cooperate to inhibit growth of mould, so that efficient attenuation is realized. The bacillus velezensis metabolizes to generate glucose, ethanol and free amino acid, and the lactobacillus plantarum metabolizes to generate ester flavor substances, so that the feed is endowed with strong fermentation aroma. Meanwhile, the pungent smell of volatile aldehyde substances is eliminated through double-bacterium synergism.

The invention belongs to the technical field of slow-release fertilizers, and relates to a slow-release microbial humic acid compound fertilizer as well as a preparation method and application thereof. The invention provides a preparation method of a slow-release microbial humic acid compound fertilizer. The preparation method comprises the following steps: calcining vermiculite to obtain expanded vermiculite; uniformly mixing expanded vermiculite, tetrabutyl titanate and water, placing at normal temperature for 48-72 hours, drying and collecting to obtain modified expanded vermiculite; uniformly mixing the modified expanded vermiculite, bacillus subtilis and bacillus mucilaginosus to obtain a mixture; stirring is kept, a sodium alginate-humic acid mixed solution is sprayed to the mixture, a 2-5 wt% calcium chloride solution continues to be sprayed into the rotary drum, a reaction is conducted for 5-15 min for crosslinking, and finally a semi-finished fertilizer product is prepared after freezing and vacuum drying; and mixing the fertilizer semi-finished product with arbuscular mycorrhizal fungi to prepare the slow-release microbial humic acid compound fertilizer. The fertilizer provided by the invention can effectively improve the morbidity of salvia miltiorrhiza during continuous cropping and improve the quality of medicinal materials.

The invention discloses a method for preparing an amino acid fertilizer by multi-strain synergistic starter propagation and a liquid enzymolysis method, which comprises the following steps: selecting 25%-35% of bacillus subtilis, 10%-20% of trichoderma reesei, 15%-25% of aspergillus oryzae, 10%-20% of lactic acid bacteria, 5%-15% of saccharomyces cerevisiae and 5%-15% of aspergillus niger as composite strains; according to the method, six strains such as bacillus subtilis and trichoderma reesei form a composite microbial system, soybean meal and bran are used as raw materials, and the problems of low degradation efficiency, unstable enzyme activity and the like of a traditional process are solved through a process of'three-stage temperature-controlled starter propagation and four-stage liquid fermentation '; the method is characterized in that a'bacillus-mould-saccharomycetes-lactic acid bacteria 'four-flora synergistic system is constructed, a starter propagation-fermentation multi-parameter coupling regulation and control process is initiated, a'process-quality-effect' closed-loop mechanism is established, compared with a traditional process, the efficiency is improved by 35%-50%, the ton cost is reduced by 15%-20%, and the product is suitable for seed treatment and foliage spraying of various crops and has wide application prospects. The industrialization potential is remarkable, and the requirements of workers are met.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

The subject invention relates to two-stage inoculation and two-stage fermentation process which are performed by the inoculation time difference and staged fermentation. In the first stage, Bacillus coagulans, lactic acid bacteria and Acetobacter aceti are inoculated into a fermentation tank to perform primary fermentation to produce primary fermented material. In the second stage, yeast is added into the primary fermented material in a container to perform the secondary fermentation to produce secondary fermented material, and then the secondary fermented material is pasteurized to deactivate yeast, lactic acid bacteria and Acetobacter aceti in the container and stop fermentation reaction. Finally, the secondary fermented material is cooled to ambient temperature to prepare the kombucha fermented beverage preserving active Bacillus coagulans at ambient temperature. The invention is help to improve the preservation convenience of the kombucha fermented beverage and avoid excessive alcohol concentration.

This invention relates to the field of bio-infectious agent metabolites, and discloses a bio-infectious agent metabolite for the safe improvement of agricultural products and its preparation method. The bio-infectious agent metabolite is obtained by fermenting a fermentation raw material composed of a carbon source and a nitrogen source with an immobilized composite microbial agent on an organic-inorganic composite carrier. The organic-inorganic composite carrier immobilized composite microbial agent is prepared by immobilizing the composite microbial agent onto a chitosan-sodium alginate organic embedding carrier and an inorganic adsorbent carrier using immobilization loading technology. The composite microbial agent consists of *Bacillus circinus*, *Bacillus licheniformis*, *Bacillus amyloliquefaciens*, and *Bacillus subtilis*. This invention provides a method for preparing bio-infectious agent metabolites, and yields a bio-infectious agent metabolite containing carbohydrate compounds, amino acid compounds, organic acid compounds, and plant hormones, which has a quality-improving effect on agricultural products.

The invention relates to the technical field of pet food, in particular to an eye-benefiting and liver-protecting fermentation type canine nutrient and a preparation method thereof, and the eye-benefiting and liver-protecting fermentation type canine nutrient comprises the following components: a liver-protecting component, an eye-benefiting component, a chitosan-calcium compound solution, beer yeast powder, honey, lactobacillus plantarum freeze-dried powder and bacillus subtilis freeze-dried powder. Porous starch of the liver protection component carries and protects N-acetylcysteine, and carnosine and quercetin modified by zein remove fermentation byproducts. The eye benefiting component is loaded and protected by octenyl succinic acid modified starch, and the dispersity and the stability of the xanthophyll are improved by grafting a hydrophobic cavity of sulfobutyl-beta-cyclodextrin. The laccase fixed on the surface of the porous starch catalytically oxidizes phenolic hydroxyl groups in a tannic acid-gelatin network on the surface of the eye benefiting component by using dissolved oxygen, a protective shell is formed by in-situ crosslinking and curing, and a chitosan-calcium compound solution closes the physical distance between the laccase and the tannic acid-gelatin network, so that the enzymatic oxidation reaction rate is increased.

The invention relates to the technical field of silage, and provides a silage additive for degrading mycotoxin and application of the silage additive. The composite microbial inoculant is composed of lactobacillus paracasei and bacillus subtilis; or the microbial agent is a composite microbial agent composed of lactobacillus paracasei, bacillus subtilis and pediococcus pentosaceus. By screening degrading bacteria aiming at ochratoxin, aflatoxin, zearalenone and vomitoxin for compounding and combination, the degrading effects of different lactic acid bacteria on various toxins are respectively exerted, the various mycotoxins are effectively degraded, and particularly, the degrading rate of the ochratoxin can reach 100%. The degradation rate of the mixed bacteria to other mycotoxins can exceed that reported in the prior art, and faster degradation or higher degradation rate can be achieved by adjusting the addition amount.

This invention discloses a salt-tolerant, alkali-tolerant, and hydrocarbon-loving composite functional microbial agent for the remediation of oil-containing saline-alkali land, its preparation method, and its application. It relates to the field of environmental microbiology and soil bioremediation technology. The composite microbial agent is composed of halophilic Bacillus, alkaliphilic Pseudomonas, and petroleum hydrocarbon-degrading Mycobacterium, compounded in a viable count ratio of 3:2:2, with a single strain having a viable count ≥ 5.0 × 10⁻⁶. 9 This invention, with a concentration of CFU / g, can tolerate extreme environments including 12% NaCl concentration and pH 9.5. Through targeted screening and precise formulation of functional bacterial strains, it achieves a synergistic effect of salt and alkali tolerance and broad-spectrum petroleum hydrocarbon degradation. It can be widely applied in oilfield ecological restoration projects, recultivation of saline-alkali polluted farmland, and in-situ bioremediation of oil- and salt-alkali co-contaminated soils. The preparation process is simple, and the bacterial cell activity retention rate is high, solving the technical problems of poor stress resistance and low degradation efficiency of traditional degrading agents. It possesses excellent remediation effects and industrial application value.

The invention belongs to the technical field of water treatment, and discloses a compound microbial agent and a preparation method and application thereof, the preparation method comprises the following steps: 1) mixing bacterial powder of photosynthetic bacteria, nitrifying bacteria, denitrifying bacteria, saccharomycetes, lactic acid bacteria, bacillus subtilis, bacillus licheniformis and actinomycetes to obtain mixed bacterial powder; 2) mixing inorganic mineral particles, an electron acceptor and a microbial growth promoting agent to obtain an auxiliary material; 3) dissolving sodium alginate and polyvinyl alcohol in water to obtain an organic carrier solution; and 4) adding the mixed bacterial powder and auxiliary materials into the organic carrier solution, and dropwise adding into a calcium chloride solution for cross-linking and curing to obtain the compound microbial agent. According to the invention, the mixed bacterial powder, the inorganic mineral particles, the electron acceptor and the microbial growth promoting agent are embedded and fixed together, so that the microbial growth promoting agent has certain mechanical strength, the capability of resisting environmental impact is improved, a good living place is provided for microorganisms, long-term slow release of the microbial growth promoting agent is realized, and the recovery of the self-cleaning capability of a water body is promoted.

The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent for increasing the content of glycogen in oysters. The microbial agent is obtained by coating bacillus, lactic acid bacteria and monad with sodium alginate, calcium chloride, calcium alginate and chitosan. According to the application, the number and activity of viable bacteria in the microbial agent are improved by adjusting the content of NaCl, the temperature and the pH value; according to the prepared microbial agent for increasing the glycogen content of the oysters, the functions of the used microorganisms are complementary, through the cooperation of immunity and metabolism, synthesis of the glycogen is increased, consumption of the glycogen is reduced, the synthesis efficiency of the glycogen in the oysters is enhanced, the glycogen content of the oysters is increased, and an innovative solution is provided for improving the quality and efficiency of oyster culture.

The application belongs to the technical field of bioactive substance preparation and agricultural application, and discloses a beta-amino butyric acid, a whole cell conversion preparation method thereof and application thereof. The method uses Paenibacillus halophilus as a catalyst to perform biological conversion in a reaction system containing a substrate, so as to obtain the beta-amino butyric acid. The method has mild reaction conditions, the bacterial cells can be repeatedly used, the conversion rate is high, and the green, efficient and low-cost production of the beta-amino butyric acid is realized. The application also provides application of the beta-amino butyric acid prepared by the above method in improving salt stress resistance of crops and in plant disease control as a pesticide synergist. Experiments show that the beta-amino butyric acid can significantly alleviate the inhibition of salt stress on the growth of wheat, and improve the chlorophyll content and biomass accumulation; the beta-amino butyric acid has a synergistic effect on grape diseases when used in combination with anilazodifen and fluoroacetamide, and meanwhile, the fruit quality and storage resistance are improved.

The application discloses a method for producing feed by using Gramineae plant waste, and comprises the following steps: S1, raw material pretreatment: after the Gramineae plant waste is dedusted, cut or crushed, water immersion and ammoniation treatment are carried out, so that the water content reaches 30-60%, and the ammonia addition amount is 3-5% of the dry weight; S2, gas phase blasting: the pretreated material is subjected to gas phase blasting, and the medium is selected from water vapor or non-reactive compressed gas; S3, microbial agent fermentation: a compound microbial agent containing at least two of lactic acid bacteria, yeast bacteria, bacillus and cellulose-decomposing bacteria is added to the blasted material, the water content is adjusted to 60-70%, and sealed fermentation is carried out for 3-7 days; S4, granulation: the fermented material is dried to have a water content of 12-18%, other raw materials are mixed after crushing, and the feed product is obtained through steam conditioning and pressing granulation. Through the cooperation of multiple processes, the method can significantly destroy the structure of the straw, improve the crude protein content and the feeding value, and the product is convenient to store and transport.

The present invention discloses a novel fructose utilizing lactic acid producing bacteria Bacillus coagulans FF-7 (MTCC 25235) and its isolation and characterization method. The present invention also discloses the biological applications / therapeutic uses of fructose utilizing lactic acid producing bacteria in increasing utilization of fructose from food and in managing diseases associated with high fructose intake.

The present disclosure relates to Lactiplantibacillus plantarum EL6 for increasing the flavonoids content, and a use thereof, and belongs to the technical field of microbial feeds. The present disclosure is intended to solve the problem that there is no special lactobacillus agent for increasing the flavonoids content during silage fermentation in the prior art. The Lactiplantibacillus plantarum EL6 in the present disclosure is a Gram-positive bacterium, which allows glucose facultative fermentation and has advantages such as acid resistance and high growth rate. In addition, the Lactiplantibacillus plantarum EL6 has an ability to allow the biotransformation of flavonoids, and can significantly increase a content of flavonoids in Medicago silage and promote the release and transformation of bound flavonoids. The Lactiplantibacillus plantarum EL6 can improve a fermentation quality of silage, and has advantages such as low cost, safety, reliability, and easy utilization.

The invention discloses a natural antiviral liquid and a preparation method and application thereof, and relates to the technical field of agricultural biotechnology and plant protection.The natural antiviral liquid is prepared through sequential mixed fermentation of bacillus subtilis and bacillus atrophaeus, corncob hydrolysate and corn steep liquor are used as a composite culture medium, and the natural antiviral liquid is prepared through fermentation. A pH response type attapulgite carrier which is accurately modified by chitosan and specific double precursors are added; the method comprises the following steps: inoculating bacillus subtilis, and inoculating bacillus atrophaeus when the pH value of fermentation liquor is naturally reduced to 6.0-6.2, thereby constructing a'metabolism interlocking 'and'signal space-time regulation' double closed-loop system. The method is simple in process and low in cost, the product synergistic interaction effect is remarkable, the prepared antiviral liquid can be self-assembled to form intelligent micelles, the inhibition effect on tobacco mosaic virus (TMV) is excellent, the lasting period is long, and the application prospect in green plant protection is wide.

The present invention relates to Lacticaseibacillus paracasei HP7 with preventive or alleviative efficacy for functional dyspepsia and uses thereof. The composition comprising the Lacticaseibacillus paracasei HP7 strain (accession number: KCTC 13143 BP) of the present invention as an active ingredient was confirmed to increase expression of intestinal tight junction genes, improve gastrointestinal peristalsis and gastric emptying effects, enhances gastric weight and gastric emptying levels associated with gastric emptying, and significantly increase blood hormone levels related to digestive activity, thereby improving dyspepsia.

The invention discloses a compound enzyme bacterium fermented agricultural and sideline product feed and a production process thereof, and relates to the technical field of biological fermentation, the compound enzyme bacterium fermented agricultural and sideline product feed is obtained by anaerobic fermentation of compound enzyme bacterium fermented agricultural and sideline products; the agricultural and sideline products are cotton straws, corn flour, cottonseed hulls, cottonseed meal and bran; the compound enzyme bacteria are obtained by mixing soft rot fungi, trichoderma, cellulase, clostridium, manganese peroxidase and lactic acid bacteria; according to the compound enzyme bacterium fermented agricultural and sideline product feed disclosed by the invention, the cotton byproducts are treated by adopting a microbial fermentation method, after microbial fermentation, straw lignin, hemicellulose, gossypol, crude fibers and other components, which are not suitable for being used as feed, in the cotton byproducts are degraded, and the anti-nutritional factor level in the feed is remarkably reduced; the nutritional value of the feed is greatly improved; the feed can be used as a total mixed ration, can be used for raising livestock such as cattle and sheep, greatly reduces the feeding cost, and solves the problem of low utilization rate of cotton by-products.

The invention provides an anti-hair-loss nourishing shampoo and a preparation method thereof, and the anti-hair-loss nourishing shampoo comprises the following raw materials by mass: 0.25-0.5 part of lipid, 0.5-1 part of a thickening agent, 0.5-1 part of a preservative, 0.5-1 part of a preservative, 0.5-1 part of an anti-aging agent, and the balance of water. 0.06 to 0.12 part of a bioactive peptide; 3-4 parts of a humectant; 2-3 parts of a herbal extract; 0.15 to 0.3 part of hydroxypropyl-beta-cyclodextrin; 0.4 to 0.7 part of trehalose; 1.5 to 2 parts of a bacillus fermentation product; 0.2 to 0.3 part of polyquaternium-10; 0.25 to 0.4 part of silk amino acid; 0.2 to 0.4 part of panthenol; 0.1 to 0.2 part of dipotassium glycyrrhizinate; 0.1 to 0.2 part of an organic salt; 2.5 to 3.5 parts of glycine betaine; 0.8 to 1 part of PEG-150 (Polyethylene Glycol) pentaerythritol tetrastearate; 1 to 1.5 parts of cocamide MEA; 10 to 13 parts of an anionic surfactant; 3-5 parts of an ampholytic surfactant; 0.4 to 0.8 part of a cosolvent; 0.2 to 0.5 part of organic acid; 0.5-1 part of sodium chloride; 1-1.6 parts of a preservative; 0.1 to 0.3 part of costus oil; and the balance of water. The anti-hair-loss nourishing shampoo can improve the stretching function, glossiness and hair density of hair bundles.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

The invention relates to a livestock and poultry feed composition which comprises lactic acid bacteria and bacillus as active ingredients and is mixed with basal feed for use, the composition is prepared by adding lactic acid bacteria and bacillus into a basal feed according to a specific proportion, and can be applied to the livestock and poultry feeding process; the preparation method comprises the steps of strain culture, mixing and drying. Tests show that the composition can be used for feeding livestock and poultry such as pigs, chickens and cattle.

The invention discloses a high-temperature-resistant and acid-resistant multifunctional compound microbial agent and application thereof in silage, and belongs to the technical field of agricultural microorganisms. The microbial agent is prepared by compounding lactobacillus CYM6 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36209, bacillus CYC6 with the preservation number of CGMCC No.36210 and Vickers ham yeast CYY6 with the preservation number of CGMCC No.35997 according to the ratio of 8: 1: 1. Through the synergistic effect of the three bacteria, the lactic acid bacteria CYM6 are used for rapidly producing acid to reduce the pH value and inhibit putrefying bacteria; cellulose degrading bacteria CYC6 are used for degrading cellulose, so that the palatability and the digestibility are improved; saccharomycetes CYY6 are used for synthesizing mycoprotein, so that the content of crude protein in the feed is increased. The microbial agent can quickly become a dominant flora in an external high-temperature environment, effectively inhibit spoilage microorganisms, reduce the pH value of silage, degrade the cellulose content of forage grass raw materials, reduce dry matter loss and protein degradation, and significantly improve the fermentation quality and nutritional value of the hybrid pennisetum silage; and an effective technical support is provided for solving the production problem of high-quality silage in southern areas.

The invention discloses a preparation process of a live microorganism fermented high-protein pet food, and relates to the technical field of animal feeds, prepared raw materials are added according to nutritional requirements, the raw materials are one or more of chicken meal, deep sea fish meal, low-temperature detoxified soybean meal and puffed soybean meal, the raw materials are subjected to live microorganism fermentation treatment, and the high-protein pet food is obtained. The live microorganisms are a combination of at least two of mould, bacillus, saccharomycetes and lactic acid bacteria, and sequential control is implemented based on synergistic fermentation of multiple live microorganisms. According to the invention, through sequential control of aerobic, facultative and anaerobic, growth characteristics of mould, bacillus, saccharomycetes and lactic acid bacteria are accurately adapted, and strain nutrition competition and metabolism antagonism are eliminated. The raw materials are enzymatically hydrolyzed by the mould, the bacillus degrades protein, the yeast optimizes flavor, the lactic acid bacteria inhibits bacteria and protects intestines, and the whole link synergistically exerts power, so that the defect that the function of a single strain is poor is avoided, and more efficient nutrition conversion and function enhancement effects are realized.

The invention discloses a microbial agent for efficiently treating organic solid waste as well as a preparation method and application of the microbial agent, and relates to the technical field of fermentation engineering. The microbial agent comprises the following components in parts by weight: 50-70 parts of bacillus subtilis, 30-50 parts of bacillus, 10-30 parts of lactic acid bacteria, 10-30 parts of trichoderma, 10-30 parts of saccharomycetes, 5-10 parts of auxiliary materials, 5-10 parts of a protective agent and 1-5 parts of an additive. The invention further provides a preparation method of the microbial inoculum and application of the microbial inoculum in efficient treatment of organic solid waste. According to the microbial agent for efficiently treating the organic solid waste and the preparation method and application of the microbial agent, the organic solid waste can be effectively degraded, the degradation rate is high, harmful substances are not generated, energy is saved, environment friendliness is achieved, meanwhile, the survival rate of strains is high, storage and use are convenient, and the problems that an existing microbial agent is low in organic solid waste degradation rate, low in cost and the like can be solved. And the survival rate is poor.