119 results about "Sporolactobacillus" patented technology

The invention discloses a preparation technology of directional debitterizing yellow tea, and belongs to the technical field of tea processing. Fresh tea leaves are used as raw materials, the yellow tea is prepared through the processes of pretreatment, fermentation, fixation, rolling, heaping for yellowing and drying, during fermentation, a compound bacteria solution and the spread and aired fresh leaves are mixed and fermented, strains in the compound bacteria solution comprise lactic acid bacteria, saccharomycetes and bacillus, and through the synergistic effect of the lactic acid bacteria, the saccharomycetes and the bacillus, the yellow tea is prepared. Balancing of directional degradation of bitter and astringent substances and generation of flavor substances is achieved, high-quality yellow tea is prepared through efficient debitterizing, the stage heaping yellowing technology is adopted, primary heaping and secondary heaping are adopted for two-stage heaping yellowing, the quality of the yellow tea is enhanced, and poor taste caused by excessive heaping yellowing is avoided. According to the finished tea prepared by the preparation process, the content of tea polyphenol is reduced to 18%-20% through detection, the content of amino acid reaches 4.5%-5.0%, bitter taste and astringent taste are relieved, sweet taste is added, and the taste is mellow.

The invention relates to the technical field of agricultural waste recycling, and discloses a formula and a preparation method of an amaranthus hypochondriacus and moso bamboo powder microbial fermentation feed, and the materials comprise amaranthus hypochondriacus straw, moso bamboo powder, corn flour, a mineral premix, salt and a microbial fermentation agent. After fermentation, the content of crude fibers is obviously reduced, the content of crude proteins is increased, the effect is optimal according to the proportion of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5), lignin in the moso bamboo powder is partially degraded, polysaccharide substances are released, the palatability of the feed is improved, the pH value of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5) in the optimal fermentation group is reduced to 4.2, the content of lactic acid reaches 2.3 g / kg, the total amount of VFA is increased, and the reproduction of harmful bacteria is inhibited; the reasonable proportion of the amaranthus hypochondriacus and the moso bamboo powder can balance carbon and nitrogen sources and promote synergistic fermentation of microorganisms, degradation of crude fibers in the fermentation process is related to acid production of lactic acid bacteria and cellulase secretion of bacillus, and the fermented feed can be used as a coarse feed substitute for ruminants or used in pig feed to improve intestinal health.

The invention discloses a mulberry leaf-vinasse fermentation composition which comprises the following raw materials: vinasse, mulberry leaves and a complex microbial inoculant, the complex microbial inoculants comprise saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum. The mulberry leaf-vinasse fermentation composition is obtained by performing anaerobic solid state fermentation on the raw materials, namely crushing vinasse and mulberry leaves to 20-50 meshes, uniformly mixing, adjusting the water content of the raw materials to be 50-70wt%, inoculating a complex microbial inoculant, uniformly stirring, filling into a breathing bag, sealing at 37 + / -0.5 DEG C, and fermenting for 4-6 days, thereby obtaining the mulberry leaf-vinasse fermentation composition. According to the mulberry leaf-vinasse fermentation composition, the mulberry leaves and the vinasse are mixed, then the complex microbial inoculant is inoculated for solid-state fermentation, the pH value of the prepared mulberry leaf-vinasse fermentation composition is remarkably reduced, meanwhile, the lactic acid content is remarkably increased, the antinutritional factor content is reduced, true protein is well preserved, the antioxidant activity is more reserved, and the fermentation effect of the mulberry leaves and the vinasse is improved; the feeding value of the mulberry leaves and the vinasse is effectively improved.

The invention discloses a special organic fertilizer for kiwi fruits and a preparation method thereof. The organic fertilizer comprises the following raw materials in parts by weight: 50-70 parts of livestock and poultry manure, 30-50 parts of kiwi fruit branch and leaf residues, 10-20 parts of soybean meal, 5-10 parts of bone meal, 3-8 parts of humic acid and 1-3 parts of a microbial agent. The microbial agent comprises bacillus subtilis, bacillus mucilaginosus, saccharomycetes and lactic acid bacteria in a ratio of 1: 1: 1: 1. The raw materials are pretreated and then subjected to a mixed fermentation process to prepare the finished product. The organic fertilizer is comprehensive in nutrition, can meet the nutritional requirements of kiwi fruit growth, improves the soil environment, improves the fruit quality and yield, and has remarkable economic benefits and environmental benefits.

The present invention discloses a process for preparing lactic acid by mechanically enhanced ammonia method from straw-like biomass. The enzymatic hydrolysate of solid fibers obtained by the mechanically enhanced ammonia method from straw-like biomass is used as the carbon source of the fermentation medium in the process of fermenting lactic acid. The preparation of the carbon source in the fermentation medium comprises the following steps: S1: treating straw-like lignocellulosic biomass with a pretreatment liquid containing ammonia water and inorganic ammonium salts in a twin-screw device; S2: subjecting the obtained material to a heat preservation reaction, and after solid-liquid separation, obtaining solid fibers containing holocellulose; S3: enzymatically hydrolyzing the obtained solid fibers with Trichoderma reesei cellulase and xylanase, and performing solid-liquid separation, and the obtained supernatant is used as the carbon source in the fermentation medium for fermenting L-lactic acid. The present invention uses the sugar liquid obtained by enzymatic hydrolysis and saccharification of pretreated holocellulose, inoculates Bacillus coagulans to ferment L-lactic acid after supplementing nitrogen source and inorganic salts, and can greatly improve the concentration and conversion rate of L-lactic acid.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention relates to the technical field of fermentation, in particular to a bean sour soup processing method based on bean clear liquid fermentation, and the processing method comprises the following steps: S1, filtering bean clear liquid, adjusting dry matter concentration, adding glucose, pasteurizing, and cooling; s2, inoculating a mixed strain of aspergillus oryzae and aspergillus sojae, and performing aerated fermentation; s3, inoculating a lactic acid bacteria mixed strain, and carrying out sealed anaerobic fermentation; s4, carrying out hot filtration, centrifugation and ultra-high temperature sterilization on the fermentation liquid, inoculating bacillus velezensis and gluconacetobacter, carrying out aeration stirring fermentation, inoculating saccharomycetes, and standing for anaerobic fermentation; and S5, carrying out solid-liquid separation on the fermentation liquor, and sterilizing to obtain the bean sour soup product. According to the method, multi-stage ordered fermentation is combined with a multi-strain synergistic effect, and parameters such as filtration, inoculation amount and temperature in each stage are optimized, so that efficient debitterizing, umami enhancement, aroma level enrichment and sour taste softening are realized, sanitation, safety and quality stability of products are guaranteed, and the taste and quality of the bean sour soup are effectively improved.

The invention discloses a high-yield caproic acid bacterium and application thereof in production of caproic acid by anaerobic fermentation of white spirit brewing yellow water. According to the invention, a high-yield caproic acid bacterium, namely bacillus aryabhattai H1 (CCTCC NO: M 20241040), is obtained through separation and screening, the high-yield caproic acid bacterium can grow and synthesize caproic acid by independently taking ethanol or lactic acid as a substrate, and the caproic acid production efficiency is optimal when ethanol and lactic acid are taken as a composite substrate. According to the bacillus aryabhattai H1, under the open type fermentation condition that a substrate does not need to be sterilized, the caproic acid can be efficiently produced by taking the white spirit brewing byproduct yellow water as the substrate, and the yield, the yield and the production intensity of the caproic acid can reach 12.5 g / L, 2.5 kg / m < 3 > / d and 22% or above respectively.

The invention relates to a method for preparing fish feed by secondary utilization of traditional Chinese medicine wastes, and belongs to the technical field of fish feed. After the traditional Chinese medicine waste is pretreated to remove heavy metal, primary fermentation of bacillus subtilis and aspergillus niger is carried out, the bacillus subtilis can degrade cellulose, the aspergillus niger decomposes antinutritional factors, then fermentation of lactobacillus lactis and candida utilis is carried out, lactic acid bacteria produce acid to inhibit infectious microbes, flavor substances are synthesized, and palatability is improved. Through traditional Chinese medicine residue pretreatment, multi-strain synergistic fermentation and a scientific feed formula, efficient reutilization of traditional Chinese medicine waste is successfully realized, and the fish feed which is rich in nutrition, high in safety and remarkable in economic benefit is produced.

The present invention discloses an application of a moisturizing coating with a flavoring function on cigarettes. The moisturizing coating comprises: a composite bacterial solution, a tobacco polysaccharide solution, and a chitosan solution. The volume ratio of the composite bacterial solution, the tobacco polysaccharide solution, and the chitosan solution is (3-4): (2-3): (4-5). The composite bacterial solution is formed by mixing lactic acid streptococci, bacillus, and yeast in a certain proportion. In the moisturizing coating of the present application, the secondary metabolites of lactic acid streptococci can inhibit miscellaneous bacteria, chitosan is a natural and harmless antibacterial film, and tobacco polysaccharides themselves have moisturizing properties. The composite bacterial solution can interact with tobacco polysaccharides and chitosan to better inhibit miscellaneous bacteria, and the protective film has a better protective effect. The metabolites of bacillus and yeast produce aroma-causing substances during the combustion and cracking process of cigarettes, thereby increasing the aroma of cigarettes.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The invention discloses a compound microbial agent and application thereof, and belongs to the technical field of microbial agents. The method specifically comprises the following steps: adding clostridium butyricum fermentation centrifugate into a fermentation tank, adding a nitrogen source, a carbon source and inorganic salt into the fermentation tank, then adding a bacillus subtilis solution and a bacillus licheniformis solution, stirring at a certain temperature and a certain rotating speed, fermenting for a period of time, then adding a lactic acid bacteria solution, and fermenting for a period of time at a certain rotating speed; stirring and fermenting at a certain rotating speed at a certain temperature, detecting the pH value after fermenting for a period of time, and when the pH value is reduced to 4.3-4.5, obtaining the compound microbial agent. The prepared compound microbial agent can improve the saline-alkali soil, effectively improve the pH of the saline-alkali soil, consume soil salinity and improve the crop germination rate.

The application discloses a Qingjiang Aurantii Fructus extract with soothing and repairing effects on atopic dermatitis and application thereof, and a preparation method of the Qingjiang Aurantii Fructus extract comprises the following steps: A1, crushing Qingjiang Aurantii Fructus, adding water to soak, and then sterilizing; A2, inoculating probiotics in the solution after sterilization in step A1 to perform fermentation; A3, filtering the obtained fermentation liquor after sterilization, and purifying to obtain the Qingjiang Aurantii Fructus extract; wherein the probiotics are at least one selected from lactic acid bacteria, bacillus and yeast fungi. The Qingjiang Aurantii Fructus extract obtained by fermenting Qingjiang Aurantii Fructus with specific probiotics has significant soothing, repairing and anti-inflammatory effects, and has the soothing and repairing effects on atopic dermatitis in particular.

The invention relates to the technical field of fertilizers, in particular to a microbial amino acid-containing water-soluble fertilizer and a preparation method thereof. The method comprises the following steps: providing a first corn steep liquor and a second corn steep liquor; adding a Trichoderma harzianum-Purpureocillium lilacinum complex microbial inoculant and lactic acid bacteria into the first corn steep liquor, and carrying out first fermentation to obtain a first fermentation broth; mixing the first fermentation liquor with the second corn steep liquor to obtain mixed corn steep liquor; adding bacillus subtilis into the mixed corn steep liquor, and carrying out second fermentation to obtain second fermentation liquor; and sequentially adding a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer into the second fermentation liquor to obtain the water-soluble fertilizer containing microbial amino acids. The microbial amino acid-containing water-soluble fertilizer prepared by the preparation method provided by the invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility, promote the root development of the crops and improve the resistance of the crops to adversity.

The invention discloses a compound microorganism fermented attenuated aroma-enhanced potato residue feed and a preparation method thereof, and belongs to the field of feed processing. The feed comprises potato residues, crop straw particles, corn flour, bran, soybean meal, urea, ammonium sulfate and a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis and lactobacillus plantarum. The preparation method comprises raw material pretreatment; preparing a microbial agent; performing mixed fermentation; and post-processing. The bacillus velezensis secretes glycosidase, and glucosidic bonds of the glycoalkaloid are subjected to enzymolysis; lactobacillus plantarum is metabolized to generate lactic acid to create an acidic environment to promote chemical degradation of glycoalkaloid, and the double bacteria cooperate to inhibit growth of mould, so that efficient attenuation is realized. The bacillus velezensis metabolizes to generate glucose, ethanol and free amino acid, and the lactobacillus plantarum metabolizes to generate ester flavor substances, so that the feed is endowed with strong fermentation aroma. Meanwhile, the pungent smell of volatile aldehyde substances is eliminated through double-bacterium synergism.

The invention discloses an artificial bait additive for improving intestinal flora of yellow fin tuna, which comprises 15-30% of fish meal, 15-30% of soybean meal, 5-10% of wheat starch, 2-5% of fish oil, 1-3% of rapeseed oil, 5-10% of mulberry leaf powder, 5-15% of fly maggot powder, 1-5% of microalgae, 0.1-0.5% of bile acid, 1-3% of pine needle meal, 5-15% of brown rice and 1-5% of cow milk casein. The Chinese herbal medicine active components comprise 0.01%-0.1% of an acanthopanax senticosus extract, 0.05%-0.5% of ophiopogon japonicus polysaccharide, 1%-5% of bitter gourd dry powder, 0.01%-0.1% of a platycodin extract and 1%-5% of a fresh peach leaf fermentation product; the probiotic complexing agent comprises the following components in percentage by weight: 0.01%-0.5% of bacillus subtilis fermentation liquor, 0.01%-0.2% of lactobacillus acidophilus freeze-dried powder and 0.01%-0.3% of bacillus licheniformis fermentation liquor, and the auxiliary components comprise 1%-5% of a brewer's grain fermentation substrate, 0.1%-0.5% of a fish oil emulsifier and 0.1%-1% of fructo-oligosaccharide. According to the present invention, the growth performance of the yellow-fin tuna can be comprehensively promoted, the immunity is enhanced, the intestinal health of the yellow-fin tuna is optimized, and the feed conversion rate can be increased.

The invention belongs to the technical field of slow-release fertilizers, and relates to a slow-release microbial humic acid compound fertilizer as well as a preparation method and application thereof. The invention provides a preparation method of a slow-release microbial humic acid compound fertilizer. The preparation method comprises the following steps: calcining vermiculite to obtain expanded vermiculite; uniformly mixing expanded vermiculite, tetrabutyl titanate and water, placing at normal temperature for 48-72 hours, drying and collecting to obtain modified expanded vermiculite; uniformly mixing the modified expanded vermiculite, bacillus subtilis and bacillus mucilaginosus to obtain a mixture; stirring is kept, a sodium alginate-humic acid mixed solution is sprayed to the mixture, a 2-5 wt% calcium chloride solution continues to be sprayed into the rotary drum, a reaction is conducted for 5-15 min for crosslinking, and finally a semi-finished fertilizer product is prepared after freezing and vacuum drying; and mixing the fertilizer semi-finished product with arbuscular mycorrhizal fungi to prepare the slow-release microbial humic acid compound fertilizer. The fertilizer provided by the invention can effectively improve the morbidity of salvia miltiorrhiza during continuous cropping and improve the quality of medicinal materials.

The invention discloses a method for preparing an amino acid fertilizer by multi-strain synergistic starter propagation and a liquid enzymolysis method, which comprises the following steps: selecting 25%-35% of bacillus subtilis, 10%-20% of trichoderma reesei, 15%-25% of aspergillus oryzae, 10%-20% of lactic acid bacteria, 5%-15% of saccharomyces cerevisiae and 5%-15% of aspergillus niger as composite strains; according to the method, six strains such as bacillus subtilis and trichoderma reesei form a composite microbial system, soybean meal and bran are used as raw materials, and the problems of low degradation efficiency, unstable enzyme activity and the like of a traditional process are solved through a process of'three-stage temperature-controlled starter propagation and four-stage liquid fermentation '; the method is characterized in that a'bacillus-mould-saccharomycetes-lactic acid bacteria 'four-flora synergistic system is constructed, a starter propagation-fermentation multi-parameter coupling regulation and control process is initiated, a'process-quality-effect' closed-loop mechanism is established, compared with a traditional process, the efficiency is improved by 35%-50%, the ton cost is reduced by 15%-20%, and the product is suitable for seed treatment and foliage spraying of various crops and has wide application prospects. The industrialization potential is remarkable, and the requirements of workers are met.

The present invention relates to a composite treatment agent for oily wastewater and its application, belonging to the technical field of wastewater treatment. The composite treatment agent for oily wastewater of the present invention is mainly composed of the following components by weight: 25-35 parts of an aluminum salt flocculant, 8-12 parts of sodium polyacrylate, 4-9 parts of ethylenediaminetetraacetic acid salt, 2-6 parts of pyrophosphate, 1-4 parts of xanthan gum, 2-5 parts of an alkali metal salt of hexadecylbenzenesulfonate, 3-8 parts of alginate, 1-2 parts of a biological agent, and 5-10 parts of rare earth-modified attapulgite; the biological agent includes yeast, Bacillus subtilis, and lactic acid bacteria. The raw materials used in the composite treatment agent for oily wastewater of the present invention are low in toxicity, can avoid secondary pollution to the environment, are easy to use, require a small amount, have a high oil removal rate, and have a very obvious water purification effect. The water quality of the treated oilfield wastewater can meet the standards for reinjection into the formation and the comprehensive water quality discharge standards, and can reduce environmental pollution.

The invention belongs to the field of microbial agents, and particularly relates to a method for regulating and controlling compost heat production of waste agaric fungus bags in a layered manner by using a complex microbial agent, which comprises the following steps: mixing crushed waste agaric fungus bags with chicken manure to obtain a fermentation raw material; the fermentation raw materials are tiled in a fermentation container in a layered mode, after each layer of fermentation raw materials is tiled, the water content is adjusted, complex microbial inoculants are evenly sprayed on the surface of each layer of fermentation raw materials, the first layer of fermentation raw materials at the bottom is provided with ureabacillus thermosphaeroides, ureabacillus thermosphaeroides and clostridium thermophile, and the second layer of fermentation raw materials at the bottom is provided with actinomycetes and lactic acid bacteria; bacillus subtilis, bacillus licheniformis and bacillus cereus are arranged on the third layer of fermentation raw materials at the bottom; saccharomycetes are arranged on the fourth layer of fermentation raw materials; and in the high-temperature period, the device is connected with a heating system through a heat exchange pipe pre-buried between the two layers of fermentation raw materials, and the device is suitable for efficient degradation and heat energy recovery of the agaric fungus bags.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

The invention relates to the technical field of ruminant stress resistance, in particular to a composition for rapidly relieving ruminant stress and promoting growth and application of the composition. The composition comprises gamma-aminobutyric acid and a preparation containing bacillus subtilis and lactobacillus acidophilus. The composition can be used for preparing products for resisting ruminant stress, improving growth performance and promoting growth. The bacillus subtilis and the lactobacillus acidophilus are used as a symbiotic bacterium system and are combined with the gamma-aminobutyric acid, so that rapid colonization of rumen bacteria is realized, oxidative stress and inflammatory response of ruminants are relieved, and the effects of rapidly relieving stress of the ruminants and promoting growth are achieved.

The subject invention relates to two-stage inoculation and two-stage fermentation process which are performed by the inoculation time difference and staged fermentation. In the first stage, Bacillus coagulans, lactic acid bacteria and Acetobacter aceti are inoculated into a fermentation tank to perform primary fermentation to produce primary fermented material. In the second stage, yeast is added into the primary fermented material in a container to perform the secondary fermentation to produce secondary fermented material, and then the secondary fermented material is pasteurized to deactivate yeast, lactic acid bacteria and Acetobacter aceti in the container and stop fermentation reaction. Finally, the secondary fermented material is cooled to ambient temperature to prepare the kombucha fermented beverage preserving active Bacillus coagulans at ambient temperature. The invention is help to improve the preservation convenience of the kombucha fermented beverage and avoid excessive alcohol concentration.

The invention provides a feed additive for increasing the content of n-3 polyunsaturated fatty acids in pork and a compound feed. The feed additive consists of spartina alterniflora powder and a bacterial enzyme additive, the bacterial enzyme additive is prepared from lactobacillus plantarum, aspergillus niger, yarrowia lipolytica, bacillus subtilis, cellulase, beta-mannase and protease; the compound feed comprises a basic ration and the feed additive. Through development and utilization of spartina alterniflora powder and practice, waste is turned into wealth, a new source of the feed additive is expanded, and the feed additive is added into daily ration, so that the digestibility of neutral detergent fibers of the daily ration is improved, the antioxidant capacity of serum is enhanced, and the contents of alpha-linolenic acid, arachidonic acid and eicosapentaenoic acid in pork are increased.

The present invention discloses an enteric Weizmannella coagulans strain RS804 and its application. The present invention provides an enteric Weizmannella coagulans strain RS804 that can efficiently utilize sugar beet sucrose. The strain has certain heat and acid resistance, good polysaccharide hydrolase activities such as cellulase, xylanase and amylase, and can produce L-lactic acid by saccharification and fermentation. In addition, the enteric Weizmannella coagulans strain RS804 can also metabolize and produce a certain amount of bacteriocin, which can effectively inhibit strains with certain competitiveness such as Priesteria gigantea and Bacillus subtilis, and help prevent and control fermentation contamination. The enteric Weizmannella coagulans strain RS804 disclosed in the present invention is of great significance for reducing the production cost of L-lactic acid fermentation and increasing the yield of L-lactic acid.

This invention relates to the field of bio-infectious agent metabolites, and discloses a bio-infectious agent metabolite for the safe improvement of agricultural products and its preparation method. The bio-infectious agent metabolite is obtained by fermenting a fermentation raw material composed of a carbon source and a nitrogen source with an immobilized composite microbial agent on an organic-inorganic composite carrier. The organic-inorganic composite carrier immobilized composite microbial agent is prepared by immobilizing the composite microbial agent onto a chitosan-sodium alginate organic embedding carrier and an inorganic adsorbent carrier using immobilization loading technology. The composite microbial agent consists of *Bacillus circinus*, *Bacillus licheniformis*, *Bacillus amyloliquefaciens*, and *Bacillus subtilis*. This invention provides a method for preparing bio-infectious agent metabolites, and yields a bio-infectious agent metabolite containing carbohydrate compounds, amino acid compounds, organic acid compounds, and plant hormones, which has a quality-improving effect on agricultural products.

The invention relates to the technical field of pet food, in particular to an eye-benefiting and liver-protecting fermentation type canine nutrient and a preparation method thereof, and the eye-benefiting and liver-protecting fermentation type canine nutrient comprises the following components: a liver-protecting component, an eye-benefiting component, a chitosan-calcium compound solution, beer yeast powder, honey, lactobacillus plantarum freeze-dried powder and bacillus subtilis freeze-dried powder. Porous starch of the liver protection component carries and protects N-acetylcysteine, and carnosine and quercetin modified by zein remove fermentation byproducts. The eye benefiting component is loaded and protected by octenyl succinic acid modified starch, and the dispersity and the stability of the xanthophyll are improved by grafting a hydrophobic cavity of sulfobutyl-beta-cyclodextrin. The laccase fixed on the surface of the porous starch catalytically oxidizes phenolic hydroxyl groups in a tannic acid-gelatin network on the surface of the eye benefiting component by using dissolved oxygen, a protective shell is formed by in-situ crosslinking and curing, and a chitosan-calcium compound solution closes the physical distance between the laccase and the tannic acid-gelatin network, so that the enzymatic oxidation reaction rate is increased.

The invention relates to the technical field of silage, and provides a silage additive for degrading mycotoxin and application of the silage additive. The composite microbial inoculant is composed of lactobacillus paracasei and bacillus subtilis; or the microbial agent is a composite microbial agent composed of lactobacillus paracasei, bacillus subtilis and pediococcus pentosaceus. By screening degrading bacteria aiming at ochratoxin, aflatoxin, zearalenone and vomitoxin for compounding and combination, the degrading effects of different lactic acid bacteria on various toxins are respectively exerted, the various mycotoxins are effectively degraded, and particularly, the degrading rate of the ochratoxin can reach 100%. The degradation rate of the mixed bacteria to other mycotoxins can exceed that reported in the prior art, and faster degradation or higher degradation rate can be achieved by adjusting the addition amount.