56 results about "Sodium Alginate Microspheres" patented technology

The invention discloses a culture method of anaerobic ammonia oxidation granular sludge with a polydopamine modified charcoal-loaded Fe-Cu sodium alginate microsphere kernel, which comprises the following steps: modifying charcoal by using polydopamine, then carrying out Fe-Cu ion loading, finally preparing sodium alginate microspheres, and taking the prepared sodium alginate microspheres as the kernel to prepare the anaerobic ammonia oxidation granular sludge with the polydopamine modified charcoal-loaded Fe-Cu sodium alginate microsphere kernel. And co-culturing the sodium alginate microspheres and activated bacillus DMF-4 in an oxygen-free environment, continuously culturing for 3 weeks, and collecting by using a gravity settling or centrifugal method to obtain the anaerobic ammonium oxidation granular sludge with the sodium alginate microsphere core. The sodium alginate microspheres serving as the core provide large density and good morphological support for sludge particles, the problem of sludge bulking is avoided, and the settling performance is improved. According to the present invention, the sludge anti-shearing performance is improved, the adsorption capacity of the slowly released and continuously slowly released iron ions on organic matters, ammonia nitrogen and heavy metals is enhanced, the sewage treatment effect is improved, and the iron ions are the biodegradable material so as not to cause secondary pollution in the use process.

The invention discloses an in-situ injectable bone repair adhesive material with a double-sequence osteogenesis promoting function as well as a preparation method and application of the in-situ injectable bone repair adhesive material. The preparation method comprises the following steps: 1) sequentially carrying out dual chemical grafting modification on hyaluronic acid by using methacrylic anhydride and dopamine hydrochloride to obtain a DA-HAMA biomacromolecule with UV curing and adhesion functions; (2) preparing a Ca < 2 + > physical cross-linked sodium alginate microsphere material loaded with an osteogenesis promoting drug by using a micro-fluidic system; and (3) preparing a DA-HAMA solution under dark and heating conditions, adding the vascular promoting factors and the drug-loaded microspheres when the DA-HAMA solution is cooled to room temperature, uniformly mixing, and carrying out centrifugal defoaming and irradiation sterilization to obtain the in-situ injectable bone repair adhesive material. The bone repair adhesive material has the functions of being degradable, injectable, capable of being adhered in a wet state, promoting blood vessels and promoting bone formation, and a brand new strategy is provided for bone defect repair.

The invention relates to hydrogel for treating ischemic brain injury and a preparation method thereof. The hydrogel is prepared from the following raw materials: methylacryloylated hyaluronic acid, methylacryloylated gelatin, curcumin loaded HA-PPS (Hydroxypropyl-Polyphenylene Sulfite) nano-micelles and dendrobium polysaccharide loaded sodium alginate microspheres. Through combination of a nanotechnology and an intelligent response material, HA-PPS is adopted to load curcumin and sodium alginate microspheres are adopted to encapsulate dendrobium polysaccharide, so that the drug activity can be more effectively protected, the drug is protected to penetrate through a blood brain barrier and be accurately delivered to a brain injury area, and targeted release of the drug is realized; the anti-oxidation and anti-inflammatory effects are better under the combined action of the curcumin and the dendrobium polysaccharides, and meanwhile, a slow release function is also achieved.

The invention relates to the technical field of high polymer materials, and provides a controllable dissolution microsphere preparation method based on sodium alginate and pectin, which comprises the following steps: obtaining a sodium alginate solution and a pectin solution, mixing the sodium alginate solution and the pectin solution based on a first preset proportion, and uniformly stirring and mixing by using preset stirring equipment, mixing a pre-constructed calcium chloride aqueous solution and absolute ethyl alcohol based on a second preset proportion, uniformly stirring and mixing through stirring equipment to obtain a cross-linking agent solution, dropwise adding the mixed gel solution into the cross-linking agent solution through a preset dropwise adding method, obtaining mixed gel beads in the cross-linking agent solution, and drying the mixed gel beads in a drying oven to obtain the hydrogel. And washing the mixed microgel beads to obtain the controllably dissolved microspheres. The invention mainly aims to solve the problems that pectin microspheres are different in shape and low in mechanical strength and are not suitable for cell culture and sodium alginate microspheres are still long in dissolving time when the concentration of a dissolving agent is high, and meanwhile, the microspheres which are high in pelletizing rate and controllable in dissolution are prepared.

The invention discloses a preparation method of a polydopamine modified biochar loaded Fe-Cu sodium alginate microsphere, which comprises the following steps: preparing biochar, modifying the biochar by using polydopamine, then loading the polydopamine modified biochar by using Fe-Cu, and finally obtaining the polydopamine modified biochar loaded Fe-Cu sodium alginate microsphere. According to the preparation method, the sodium alginate microspheres with different particle sizes can be prepared, the prepared sodium alginate microspheres have the function of adsorbing ammonia nitrogen, nitrate nitrogen, chlorobenzene, phosphorus, chromium ions and lead ions, the sodium alginate microspheres are used as the inner core to prepare anaerobic ammonia oxidation granular sludge, large density and good morphological support are provided for sludge particles, the problem of sludge bulking is avoided, and the anaerobic ammonia oxidation granular sludge can be prepared by using the sodium alginate microspheres as the inner core. The sewage treatment agent has the advantages that the settling performance is improved, the adsorption capacity on organic matters, ammonia nitrogen and heavy metals is enhanced, the sewage treatment effect is improved, and meanwhile, the sewage treatment agent is a biodegradable material and does not cause secondary pollution in the use process.

The invention belongs to the technical field of drug delivery, and particularly relates to a multi-response colitis disease targeting hydrogel microsphere as well as a preparation method and application thereof. The hydrogel microsphere comprises a calcium ion cross-linked sodium alginate microsphere and HA-Cys / Cur NPs which is encapsulated in the calcium ion cross-linked sodium alginate microsphere, the preparation method comprises the following steps: self-assembling cysteine ethyl ester modified hyaluronic acid and curcumin to form nano-particles, encapsulating HA-Cys / Cur NPs into sodium alginate microspheres by an emulsification method, and carrying out calcium ion crosslinking, so as to obtain the hydrogel. The hydrogel microspheres have pH and GSH response characteristics and hyaluronic acid-mediated active targeting capability, are stable in a gastric acid environment, are accurately released at a colon part and target macrophages at an inflammation part, and NPs disconnects disulfide bonds and releases curcumin in an intracellular high GSH environment, so that synergistic treatment of anti-inflammation, anti-oxidation and intestinal mucosa repair is realized, and the curative effect is good. The medicine has a good application prospect in treatment of ulcerative colitis.

The present invention discloses a chloroplast-sodium alginate microsphere and its preparation method and use. The preparation method of the chloroplast-sodium alginate microsphere comprises: mixing chloroplasts, sodium alginate and distilled water to obtain a mixed solution of chloroplasts and sodium alginate as a dispersed phase; dissolving a nonionic surfactant and CaI2 or CaCl2 in liquid paraffin to form an oil phase as a continuous phase; the dispersed phase and the continuous phase are cross-linked under the operation of a microfluidic device to form chloroplast-sodium alginate microspheres. The chloroplast-sodium alginate microspheres of the present invention have the ability to generate oxygen under illumination and are sustainable for up to two weeks. Chloroplasts are immunomodulated by reprogramming sugar metabolism to achieve anti-inflammatory and repair-promoting effects. The chloroplast-sodium alginate microspheres are suitable for wound repair, especially for chronic hypoxic wound repair similar to diabetic wounds.

The invention discloses an echinacea micromolecule peptide composition for promoting yield increase of natural calculus bovis. The echinacea micromolecule peptide composition comprises four sections of micromolecule peptides of peptide sequences A, B, C and D, the composition is extracted from echinacea through a specific process, and the proportion of each peptide sequence is 15-35%. When the sodium alginate microsphere carrier is prepared into a solution to be injected or loaded on the sodium alginate microsphere carrier to be implanted near the gallbladder of cattle, the formation rate of the natural calculus bovis can be remarkably improved to 60-65%, the average weight is greatly increased, and cholecystitis can be relieved. Compared with a traditional method, the technology is safe, efficient, wide in raw material source, low in cost and capable of specifically acting on bezoar to form a key target spot, and bezoar formation is promoted through cooperation of multiple links.

The invention provides a pH-responsive on-demand drug release double-layer hydrogel patch and a preparation method thereof, and relates to the technical field of medicines.The preparation method comprises the following steps that curcumin-loaded sodium alginate microspheres are prepared through a microfluidic device; preparing drug release layer hydrogel: dissolving acrylamide and tannic acid in deionized water, adding the prepared curcumin-loaded sodium alginate microspheres, carrying out ultrasonic treatment to uniformly disperse the curcumin-loaded sodium alginate microspheres, adding a cross-linking agent and an initiator after the dispersion is finished, and after the curcumin-loaded sodium alginate microspheres are completely dissolved, putting the curcumin-loaded sodium alginate microspheres into a drying oven for incubation to obtain the drug release layer hydrogel; and preparing the double-layer hydrogel patch: uniformly mixing a PDMS component of polydimethylsiloxane and a curing agent, casting the mixture on the prepared hydrogel of the drug release layer, then putting the mixture into a drying oven for curing, and forming a protective layer on the drug release layer to obtain the double-layer hydrogel patch. The double-layer hydrogel patch prepared by the invention can respectively release different medicines for resisting bacteria and inflammation based on the pH value of the wound, so that the wound has a better healing effect.

The invention discloses a method for preparing sodium alginate / silicon dioxide core-shell microspheres by a template method, which comprises the following steps: (1) preparing sodium alginate microspheres: preparing sodium alginate microspheres with different sizes as template cores coated by a silicon dioxide layer according to the required size by using a microsphere preparation instrument, and preparing the sodium alginate microspheres by using a template method; and (2) coating of a silicon dioxide shell layer: putting the sodium alginate microspheres in the step (1) into an aqueous solution containing tetraethoxysilane (TEOS), and carrying out in-situ polymerization on the surfaces of the sodium alginate microspheres through hydrolysis and self polycondensation of the TEOS to generate the silicon dioxide layer so as to obtain the sodium alginate / silicon dioxide core-shell structure microspheres with different sizes. The method does not depend on ethanol catalysis in the preparation process, so that the condensation reaction between silicon hydroxyl and hydroxyl in sodium alginate is weakened, a silicon dioxide coating is formed through the hydrolysis effect of TEOS in a water phase system and polycondensation between silicon hydroxyl, the surface of the sodium alginate microsphere is coated with the silicon dioxide coating, and the surface of the sodium alginate microsphere is coated with the silicon dioxide coating. Controllable preparation of sodium alginate / silicon dioxide core-shell microspheres of different sizes can be achieved, and in addition, the influence of the sizes of the core-shell structure microspheres on the adsorption performance of the microspheres is further studied.

The present application provides a double-layer hydrogel patch that releases drugs on demand in response to pH and a preparation method thereof, which relates to the field of medical technology. The preparation method comprises the following steps: using a microfluidic device to prepare sodium alginate microspheres loaded with curcumin; preparing a drug-release layer hydrogel: dissolving acrylamide and tannic acid in deionized water, adding the prepared sodium alginate microspheres loaded with curcumin and ultrasonically dispersing them uniformly, adding a cross-linking agent and an initiator after the dispersion is completed, and incubating them in an oven after they are completely dissolved to obtain a drug-release layer hydrogel; preparing a double-layer hydrogel patch: uniformly mixing the PDMS component of polydimethylsiloxane and a curing agent, casting them on the prepared drug-release layer hydrogel, and then putting them in an oven for curing, forming a protective layer on the drug-release layer, and obtaining a double-layer hydrogel patch. The double-layer hydrogel patch prepared in the present application can release different drugs for antibacterial and anti-inflammatory effects based on the pH value of the wound, so that the wound has a better healing effect.

The invention relates to the technical field of drug carriers, and discloses a sodium alginate microsphere and a preparation method and application thereof.The preparation method comprises the steps that a suspension containing cellulose and sodium alginate is added into an oil phase to be emulsified, a water-in-oil emulsion is formed, then a cross-linking agent is stirred and mixed to be subjected to a cross-linking reaction with the water-in-oil emulsion, original microspheres are obtained, and the sodium alginate microsphere is prepared. Finally, the original microspheres are treated with etching liquid, and the sodium alginate microspheres are obtained. According to the water-in-oil emulsion, cellulose cannot be dissolved in water and oil, so that cellulose in the water-in-oil emulsion is dispersed in liquid drops in a suspended state; in the cross-linking reaction process, cellulose does not participate in cross-linking and is embedded into the interior and the outer surface of a sodium alginate skeleton formed by the cross-linking reaction; after the original microspheres are treated with an etching solution, cellulose is degraded to different degrees, so that pore channels are reserved in the microspheres, the finally prepared sodium alginate microspheres are rich in pore channels, large in specific surface area and small in density, and the drug loading rate of the sodium alginate microspheres is increased.

The invention discloses a selenium-rich spirulina culture method, and belongs to the technical field of spirulina culture, the method comprises the following steps: curing spirulina by using dopamine modified sodium alginate microspheres containing magnetic particles, and carrying out two-stage culture in a culture solution with low selenium concentration and high selenium concentration containing hexanoyl-L-homoserine lactone to obtain the selenium-rich spirulina. The selenium-rich spirulina obtained through culture is high in total selenium content and organic selenium content and high in organic selenium conversion rate.

The invention discloses a construction method of a 3D printing liver organoid scaffold of FH1-loaded sodium alginate microsphere assembled mesenchymal stem cells. The construction method comprises the following steps: preparing CaO2 (at) ZIF-8 (at) SL; preparing sodium alginate microspheres FH1 (at) SA carrying FH1; and preparing the 3D printing liver organ stent. The 3D printing liver organ-like scaffold prepared by the method can significantly enhance the differentiation of human umbilical cord mesenchymal stem cells to functional stem cells, and can be used for constructing a human liver organ-like model.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses a tranexamic acid tablet capable of improving bioavailability and a preparation method of the tranexamic acid tablet. The tranexamic acid tablet is prepared from the following components in parts by weight: 50 to 65 parts of tranexamic acid, 15 to 25 parts of hydroxypropyl-beta-cyclodextrin, 5 to 8 parts of poloxamer 407, 3 to 5 parts of sodium alginate microspheres, 2 to 4 parts of carboxymethyl starch sodium and 0.5 to 1 part of stabilizer. The structure of the stabilizer is as follows: # imgabs0: tranexamic acid and hydroxypropyl-beta-cyclodextrin form an inclusion compound through a molecular inclusion technology, and the dissolution rate of the medicine is synergistically improved by combining the micelle solubilization effect of poloxamer 407. 0.5%-1% of magnesium stearate pre-coated sodium alginate microspheres are adopted to construct a pH response controlled release system, and the stability of the medicine is guaranteed through the anti-oxidation effect in cooperation with an independently researched and developed stabilizer. Animal experiments show that the bioavailability reaches 71.6%-83.2% and is improved by 40% or above compared with a traditional preparation, and the technical problems that an existing tablet is serious in oxidative degradation and low in absorption efficiency are solved.

The application discloses a construction method of a 3D printing liver organoid support loaded with mesenchymal stem cells assembled by FH1 sodium alginate microspheres, and comprises the following steps: preparing CaO2@ZIF-8@SL; preparing FH1-loaded sodium alginate microspheres FH1@SA; and preparing a 3D printing liver organoid support. The 3D printing liver organoid support prepared by the method can significantly enhance the differentiation of human umbilical cord mesenchymal stem cells into functional stem cells, and can be used for constructing a human liver organoid model.

The present invention relates to a hydrogel for the treatment of ischemic brain injury and a preparation method thereof. The hydrogel comprises the following raw materials: methacrylated hyaluronic acid, methacrylated gelatin, HA-PPS nanomicelles loaded with curcumin, and sodium alginate microspheres loaded with dendrobium officinale polysaccharide. By combining nanotechnology and intelligent response materials, the present invention uses HA-PPS to load curcumin and sodium alginate microspheres to encapsulate dendrobium officinale polysaccharide, which can more effectively protect the drug activity, protect the drug from crossing the blood-brain barrier, accurately deliver it to the brain injury area, and achieve targeted release of the drug; moreover, the antioxidant and anti-inflammatory effects of curcumin and dendrobium officinale polysaccharide acting together are better, and at the same time, it also has a sustained release function.

The application discloses a drug-loaded microalgae motor system for colon-targeted delivery and a preparation method and application thereof. The drug-loaded microalgae motor system comprises a Chlamydomonas reinhardtii microalgae motor core, the surface of the core is adsorbed with amino-modified ferroferric oxide nanoparticles through electrostatic adsorption, is wrapped with quercetin-modified chitosan quaternary ammonium salt, forms a nano-composite with magnetic responsiveness, and is encapsulated in a sodium alginate microsphere. The application utilizes Chlamydomonas reinhardtii as an antioxidant for adjuvant therapy, in a colon environment, the sodium alginate microsphere can be degraded in response to specific conditions, release the microalgae motor to autonomously move in intestinal fluid, and under the guidance of an external magnetic field, precisely target a lesion site in the intestinal tract. The application has good biocompatibility, biodegradability, gastric acid avoidance ability and magnetic / chemical dual driving targeting function, can effectively improve the treatment effect on colon diseases, and significantly reduce the systemic side effects of drugs, and expands the application of micro / nano motors in the biomedical field.

The invention discloses a preparation method of protein-embedded sodium alginate microspheres and application of the protein-embedded sodium alginate microspheres in aquaculture. According to the preparation method, after the prepared sodium alginate microspheres are used for feeding fish, the embedded protein can be prevented from being digested by gastric juice and is digested and absorbed in intestinal tracts, so that the problem of waste caused by feeding of protein or polypeptide active substances through digestive tracts is solved, efficient utilization of the protein or polypeptide active substances is realized, and the utilization rate of the protein or polypeptide active substances is increased. The method can be used for embedding various protein or polypeptide active substances. After tilapia mossambica is fed with the sodium alginate microspheres embedded with the functional neuropeptide UII, the sodium alginate microspheres can protect the functional neuropeptide, can be prevented from being digested by gastric juice, and can be digested and absorbed in intestinal tracts. After tilapia mossambica is fed with the UII embedded with sodium alginate, expression of tilapia mossambica reproduction related genes is improved, gonad development of male and female tilapia mossambica is remarkably promoted, and the UII has a good application prospect in fish culture and breeding.

The application discloses a pH response type coating agent and application thereof in seed germination of alkali grass. The application comprises the following steps: pretreating alkali grass seeds with a mixed salt alkali solution with a specific ratio, coating the seeds with a double-layer coating agent composed of an outer layer of dimethylaminoethyl methacrylate-acrylic acid copolymer and an inner layer of sodium alginate microspheres loaded with Bacillus subtilis GB03, and culturing the seeds under alternating light and darkness and variable temperature conditions; when the environmental pH reaches a critical value, the coating agent precisely swells and ruptures to release live bacteria, and the salt alkali solution activates the bacteria, thereby significantly improving the seed germination rate of the alkali grass and effectively solving the technical problem of low seed germination rate in the reconstruction of vegetation in saline-alkali soil. The method for efficiently germinating alkali grass is simple and easy to implement, and does not require complex operations, and can quickly and effectively improve the seed germination rate of the alkali grass. The method can be applied to production, thereby solving the problem of low seed germination rate of the alkali grass in the production process, and has important significance for the construction of artificial grassland of the alkali grass.

The invention belongs to the technical field of medicine, and discloses a dressing for treating diabetic foot ulcer, a preparation method and application. The dressing is characterized in that PLGA nanoparticles loaded with deferasirox are fixed on the inner walls of meshes of a silicone mesh stent through a polydopamine adhesion layer; the sodium alginate microspheres encapsulating the WAY 316606 are embedded into the carboxymethyl cellulose matrix, and the surface of the deferasirox-loaded PLGA nanoparticle layer is coated with the sodium alginate microspheres; and after the poloxamer 407 temperature-sensitive hydrogel containing rhPOSTN protein is molded by casting, the poloxamer 407 temperature-sensitive hydrogel containing rhPOSTN protein is coated with the sodium alginate microsphere layer for encapsulating WAY 316606. The thought that skin POSTN + fibroblasts regulate and control diabetic foot skin injury re-healing through iron metabolism regulation and interaction of the skin POSTN + fibroblasts and endothelial cells is proved, a molecular mechanism is disclosed, and a powerful basis is provided for later research.

The present invention relates to a circadian rhythm regulation-based pH responsive anti-decline microsphere composition and application thereof, the circadian rhythm regulation-based pH responsive anti-decline microsphere composition comprises an inner sodium alginate microsphere and an outer chitosan coating layer, the inner sodium alginate microsphere encapsulates berberine and hyaluronic acid; the outer chitosan coating layer is used for coating nicotinamide, resveratrol and the inner sodium alginate microspheres. The pH response type anti-decline microsphere composition is applied to a skin care product. Compared with the prior art, the preparation method has the advantages that the circadian rhythm adaptive release of active ingredients is realized by constructing a pH response type double-layer microsphere structure of'sodium alginate on the inner layer and chitosan on the outer layer '.

The application discloses sulfhydrylated oxidized guar gum / sodium alginate microspheres and a preparation method and application thereof, and belongs to the field of biomedical materials. The sulfhydrylated oxidized guar gum and a sodium alginate base material are used to prepare and construct microspheres with pH response, colon targeting and intestinal adhesion by an emulsion gel method, the microsphere technology is used to embed probiotics, and the survival rate of the probiotics in gastric juice is improved. Through in-vitro verification, the method of embedding the microspheres successfully prevents the direct contact of the probiotics with a harsh environment, improves the ability of the probiotics to resist strong acid in the gastrointestinal tract, and enhances the adhesion and colonization effect of the probiotics in the intestinal tract. Through an animal experiment of simulating colitis, the LGG microspheres have the best treatment effect on colitis. Compared with a large number of side effects caused by antibiotic therapy, the treatment method has a certain clinical application prospect as a means with biological safety to treat inflammatory bowel disease.

The present application belongs to the technical field of biological medicine, and particularly relates to a sodium alginate microsphere co-loading nano-gold probiotics and curcumin albumin nanoparticles and preparation and application thereof. The present application synthesizes nano-gold by modifying probiotics Escherichia coli Nissle 1917, prepares curcumin albumin nanoparticles by using the principle of self-assembly, and then prepares the sodium alginate microsphere co-loading nano-gold probiotics and curcumin albumin nanoparticles by using the method of high-voltage electrostatic, which is used for the treatment of inflammatory bowel disease. Compared with the traditional preparation of nano-gold, the present application proposes a new green synthesis method, while keeping the activity of probiotics. In order to improve the problem of poor water solubility of curcumin, the present application uses albumin to load drugs, prepares curcumin albumin nanoparticles, and then combines with the sodium alginate microsphere, which has colon targeting, improves the bioavailability of probiotics and curcumin, and is effectively used for the treatment of inflammatory bowel disease.

The invention provides a composition for treating anemia and a preparation method thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: carrying out water extraction and alcohol precipitation on caulis spatholobi, angelica sinensis and pseudo-ginseng to obtain compound polysaccharide, fermenting solid residues through iron-rich saccharomycetes and lactobacillus plantarum, separating to obtain zymophyte and a fermented product, carrying out enzymolysis on the zymophyte by using helicase and lysozyme to obtain an iron-rich lysozyme yeast product, activating succinic acid, coupling with lactoferrin and vitamin C, complexing iron, and carrying out freeze-drying to obtain the iron-rich lysozyme yeast product. And mixing the lactoferrin succinic acid / vitamin C iron with a fermentation product and an iron-rich bacteria-dissolving yeast product, and embedding the mixture in carboxylation-modified compound polysaccharide and sodium alginate microspheres to prepare the composition for treating anemia. The iron-supplementing gestational women iron-supplementing tablet has high bioavailability, basically has no stimulation effect on gastrointestinal mucosa, is high in safety, has obvious effects of rapidly supplementing iron for anemia of the gestational women and promoting bone marrow blood regeneration, and has a wide application prospect.

The invention belongs to the technical field of microbial agents, and particularly relates to a charcoal-based nitrogen-fixing bacterium agent as well as a preparation method and application thereof. According to the invention, a biochar-humic acid-sodium alginate embedding mode is adopted, so that a porous protection cabin can be provided to adsorb plant rhizosphere nutrients. Meanwhile, a low-oxygen microcell is created to protect nitrogenase, physical protection is provided for thalli, and external environmental stress is buffered. Wherein humic acid is used as a high-quality organic carbon source and is beneficial to stimulating thalli to rapidly proliferate in embedded particles and rhizosphere, and a large number of active cells can be obtained in a short time. The biochar-based nitrogen-fixing bacterium agent (namely biochar-humic acid-sodium alginate microspheres) prepared by the invention has the advantages of simple and convenient preparation method, stable storage performance and the like, and compared with other biological agents, the biomass of the dried microbial agent reaches 2.2 * 10 < 10 > CFU / g, so that the biochar-based nitrogen-fixing bacterium agent has a good application prospect.

The invention relates to the technical field of biomedical materials, and discloses an injectable biocompatible hydrogel based on dynamic supramolecular assembly, and the hydrogel comprises the following components by weight: 3.0-5.0% of zinc oxide nanocrystalline regenerated cellulose; 3.0%-5.0% of chondroitin sulfate; 1.5% to 2.5% of recombinant human source collagen; 1%-2% of beta-sodium glycerophosphate; 0.7%-1% of a cyclodextrin polymer; 0.4%-0.7% of an adamantane derivative; 0.3% to 0.5% of PEI-DNA nano particles; 0.1% to 0.3% of calcium citrate; 0.1% to 0.2% of sodium alginate microspheres; 0.15%-0.2% of a pH buffer agent; the preparation method comprises the following steps: taking collagen as a raw material, taking sterile deionized water and normal saline as residual components, and adjusting the components through subject-object supramolecular interaction, ionic crosslinking, collagen thermosensitive assembly and a step-by-step mixing process and structure, so that the prepared hydrogel has the advantages of dynamic reversible crosslinking, shear thinning and self-healing synergy, high biocompatibility and controllable degradation rate.

The invention discloses an animal feather keratin extraction method which comprises the following steps: (1) mixing pretreated animal feathers with a tea saponin solution, and stirring to obtain degreased feathers; mixing the degreased feathers with an ethanol solution, and performing ultrasonic treatment to obtain loose feathers; (2) preparing liquid drops from the water phase and the oil phase through a micro-fluidic chip process, standing and curing the collected liquid drops, demulsifying and cleaning to obtain sodium alginate-CaCl2 microspheres; (3) mixing the loose feathers, water, the sodium alginate-CaCl2 microspheres and keratinase, then adjusting the pH value, and carrying out enzymolysis; finally, taking supernate; and (4) adjusting the pH value of the supernate, precipitating, dialyzing the precipitated mixed solution, and freeze-drying to obtain the animal feather keratin. According to the method disclosed by the invention, the slow release of calcium ions is realized by adding the sodium alginate-CaCl2 microspheres, so that the enzyme utilization rate is improved.

The present invention discloses a method for preparing sodium alginate / silica core-shell microspheres by a template method, and the method comprises the following steps: (1) Preparation of sodium alginate microspheres: By using a microsphere preparation instrument, sodium alginate microspheres of different sizes are prepared according to the required dimensions as the template cores for subsequent silica layer coating; (2) Coating of the silica shell layer: The sodium alginate microspheres in step (1) are placed in an aqueous solution containing tetraethoxysilane (TEOS), and a silica layer is in-situ polymerized on the surface of the sodium alginate microspheres through hydrolysis and self-condensation of TEOS to obtain sodium alginate / silica core-shell structure microspheres of different sizes. In the preparation process of this method, ethanol catalysis is not relied on, thereby weakening the condensation reaction between silanol groups and hydroxyl groups in sodium alginate. Through the hydrolysis of TEOS in the aqueous phase system and the self-condensation between silanol groups, a silica coating is formed and coated on the surface of the sodium alginate microspheres, and the controllable preparation of sodium alginate / silica core-shell microspheres of different sizes can be realized. In addition, the present invention also studies the influence of the size of the core-shell structure microspheres on their adsorption performance.

The invention discloses an inulin gel compound for nitric oxide delivery and application of the inulin gel compound in treatment of inflammatory bowel diseases, and the compound takes prebiotic inulin as a gel matrix and is loaded with zinc-cerium nano-enzyme and arginine-sodium alginate microspheres. A new strategy is provided for nitric oxide targeted delivery and comprehensive treatment of the inflammatory bowel disease, in the colon focus position, the system releases active components through a flora / pH dual response mechanism, zinc-cerium nano-enzyme catalyzes arginine to react to generate endogenous nitric oxide, excessive active oxygen is synchronously removed, and the endogenous nitric oxide can be effectively released. Further, targeted efficient delivery of nitric oxide is realized, intestinal inflammation is effectively relieved, and intestinal flora balance and intestinal barrier repair are promoted; meanwhile, the compound reduces the whole body and neuroinflammation level by inhibiting lipopolysaccharide from entering blood, so that anxiety and depression-like behaviors accompanied by the inflammatory bowel disease are improved.