41 results about "Sodium Alginate Microspheres" patented technology

The invention belongs to the technical field of drug delivery, and particularly relates to a multi-response colitis disease targeting hydrogel microsphere as well as a preparation method and application thereof. The hydrogel microsphere comprises a calcium ion cross-linked sodium alginate microsphere and HA-Cys / Cur NPs which is encapsulated in the calcium ion cross-linked sodium alginate microsphere, the preparation method comprises the following steps: self-assembling cysteine ethyl ester modified hyaluronic acid and curcumin to form nano-particles, encapsulating HA-Cys / Cur NPs into sodium alginate microspheres by an emulsification method, and carrying out calcium ion crosslinking, so as to obtain the hydrogel. The hydrogel microspheres have pH and GSH response characteristics and hyaluronic acid-mediated active targeting capability, are stable in a gastric acid environment, are accurately released at a colon part and target macrophages at an inflammation part, and NPs disconnects disulfide bonds and releases curcumin in an intracellular high GSH environment, so that synergistic treatment of anti-inflammation, anti-oxidation and intestinal mucosa repair is realized, and the curative effect is good. The medicine has a good application prospect in treatment of ulcerative colitis.

The invention discloses an echinacea micromolecule peptide composition for promoting yield increase of natural calculus bovis. The echinacea micromolecule peptide composition comprises four sections of micromolecule peptides of peptide sequences A, B, C and D, the composition is extracted from echinacea through a specific process, and the proportion of each peptide sequence is 15-35%. When the sodium alginate microsphere carrier is prepared into a solution to be injected or loaded on the sodium alginate microsphere carrier to be implanted near the gallbladder of cattle, the formation rate of the natural calculus bovis can be remarkably improved to 60-65%, the average weight is greatly increased, and cholecystitis can be relieved. Compared with a traditional method, the technology is safe, efficient, wide in raw material source, low in cost and capable of specifically acting on bezoar to form a key target spot, and bezoar formation is promoted through cooperation of multiple links.

The invention discloses a selenium-rich spirulina culture method, and belongs to the technical field of spirulina culture, the method comprises the following steps: curing spirulina by using dopamine modified sodium alginate microspheres containing magnetic particles, and carrying out two-stage culture in a culture solution with low selenium concentration and high selenium concentration containing hexanoyl-L-homoserine lactone to obtain the selenium-rich spirulina. The selenium-rich spirulina obtained through culture is high in total selenium content and organic selenium content and high in organic selenium conversion rate.

The invention discloses a construction method of a 3D printing liver organoid scaffold of FH1-loaded sodium alginate microsphere assembled mesenchymal stem cells. The construction method comprises the following steps: preparing CaO2 (at) ZIF-8 (at) SL; preparing sodium alginate microspheres FH1 (at) SA carrying FH1; and preparing the 3D printing liver organ stent. The 3D printing liver organ-like scaffold prepared by the method can significantly enhance the differentiation of human umbilical cord mesenchymal stem cells to functional stem cells, and can be used for constructing a human liver organ-like model.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses a tranexamic acid tablet capable of improving bioavailability and a preparation method of the tranexamic acid tablet. The tranexamic acid tablet is prepared from the following components in parts by weight: 50 to 65 parts of tranexamic acid, 15 to 25 parts of hydroxypropyl-beta-cyclodextrin, 5 to 8 parts of poloxamer 407, 3 to 5 parts of sodium alginate microspheres, 2 to 4 parts of carboxymethyl starch sodium and 0.5 to 1 part of stabilizer. The structure of the stabilizer is as follows: # imgabs0: tranexamic acid and hydroxypropyl-beta-cyclodextrin form an inclusion compound through a molecular inclusion technology, and the dissolution rate of the medicine is synergistically improved by combining the micelle solubilization effect of poloxamer 407. 0.5%-1% of magnesium stearate pre-coated sodium alginate microspheres are adopted to construct a pH response controlled release system, and the stability of the medicine is guaranteed through the anti-oxidation effect in cooperation with an independently researched and developed stabilizer. Animal experiments show that the bioavailability reaches 71.6%-83.2% and is improved by 40% or above compared with a traditional preparation, and the technical problems that an existing tablet is serious in oxidative degradation and low in absorption efficiency are solved.

The application discloses a construction method of a 3D printing liver organoid support loaded with mesenchymal stem cells assembled by FH1 sodium alginate microspheres, and comprises the following steps: preparing CaO2@ZIF-8@SL; preparing FH1-loaded sodium alginate microspheres FH1@SA; and preparing a 3D printing liver organoid support. The 3D printing liver organoid support prepared by the method can significantly enhance the differentiation of human umbilical cord mesenchymal stem cells into functional stem cells, and can be used for constructing a human liver organoid model.

The present invention relates to a hydrogel for the treatment of ischemic brain injury and a preparation method thereof. The hydrogel comprises the following raw materials: methacrylated hyaluronic acid, methacrylated gelatin, HA-PPS nanomicelles loaded with curcumin, and sodium alginate microspheres loaded with dendrobium officinale polysaccharide. By combining nanotechnology and intelligent response materials, the present invention uses HA-PPS to load curcumin and sodium alginate microspheres to encapsulate dendrobium officinale polysaccharide, which can more effectively protect the drug activity, protect the drug from crossing the blood-brain barrier, accurately deliver it to the brain injury area, and achieve targeted release of the drug; moreover, the antioxidant and anti-inflammatory effects of curcumin and dendrobium officinale polysaccharide acting together are better, and at the same time, it also has a sustained release function.

The application discloses a drug-loaded microalgae motor system for colon-targeted delivery and a preparation method and application thereof. The drug-loaded microalgae motor system comprises a Chlamydomonas reinhardtii microalgae motor core, the surface of the core is adsorbed with amino-modified ferroferric oxide nanoparticles through electrostatic adsorption, is wrapped with quercetin-modified chitosan quaternary ammonium salt, forms a nano-composite with magnetic responsiveness, and is encapsulated in a sodium alginate microsphere. The application utilizes Chlamydomonas reinhardtii as an antioxidant for adjuvant therapy, in a colon environment, the sodium alginate microsphere can be degraded in response to specific conditions, release the microalgae motor to autonomously move in intestinal fluid, and under the guidance of an external magnetic field, precisely target a lesion site in the intestinal tract. The application has good biocompatibility, biodegradability, gastric acid avoidance ability and magnetic / chemical dual driving targeting function, can effectively improve the treatment effect on colon diseases, and significantly reduce the systemic side effects of drugs, and expands the application of micro / nano motors in the biomedical field.

The invention discloses a preparation method of protein-embedded sodium alginate microspheres and application of the protein-embedded sodium alginate microspheres in aquaculture. According to the preparation method, after the prepared sodium alginate microspheres are used for feeding fish, the embedded protein can be prevented from being digested by gastric juice and is digested and absorbed in intestinal tracts, so that the problem of waste caused by feeding of protein or polypeptide active substances through digestive tracts is solved, efficient utilization of the protein or polypeptide active substances is realized, and the utilization rate of the protein or polypeptide active substances is increased. The method can be used for embedding various protein or polypeptide active substances. After tilapia mossambica is fed with the sodium alginate microspheres embedded with the functional neuropeptide UII, the sodium alginate microspheres can protect the functional neuropeptide, can be prevented from being digested by gastric juice, and can be digested and absorbed in intestinal tracts. After tilapia mossambica is fed with the UII embedded with sodium alginate, expression of tilapia mossambica reproduction related genes is improved, gonad development of male and female tilapia mossambica is remarkably promoted, and the UII has a good application prospect in fish culture and breeding.

The application discloses a pH response type coating agent and application thereof in seed germination of alkali grass. The application comprises the following steps: pretreating alkali grass seeds with a mixed salt alkali solution with a specific ratio, coating the seeds with a double-layer coating agent composed of an outer layer of dimethylaminoethyl methacrylate-acrylic acid copolymer and an inner layer of sodium alginate microspheres loaded with Bacillus subtilis GB03, and culturing the seeds under alternating light and darkness and variable temperature conditions; when the environmental pH reaches a critical value, the coating agent precisely swells and ruptures to release live bacteria, and the salt alkali solution activates the bacteria, thereby significantly improving the seed germination rate of the alkali grass and effectively solving the technical problem of low seed germination rate in the reconstruction of vegetation in saline-alkali soil. The method for efficiently germinating alkali grass is simple and easy to implement, and does not require complex operations, and can quickly and effectively improve the seed germination rate of the alkali grass. The method can be applied to production, thereby solving the problem of low seed germination rate of the alkali grass in the production process, and has important significance for the construction of artificial grassland of the alkali grass.

The invention belongs to the technical field of medicine, and discloses a dressing for treating diabetic foot ulcer, a preparation method and application. The dressing is characterized in that PLGA nanoparticles loaded with deferasirox are fixed on the inner walls of meshes of a silicone mesh stent through a polydopamine adhesion layer; the sodium alginate microspheres encapsulating the WAY 316606 are embedded into the carboxymethyl cellulose matrix, and the surface of the deferasirox-loaded PLGA nanoparticle layer is coated with the sodium alginate microspheres; and after the poloxamer 407 temperature-sensitive hydrogel containing rhPOSTN protein is molded by casting, the poloxamer 407 temperature-sensitive hydrogel containing rhPOSTN protein is coated with the sodium alginate microsphere layer for encapsulating WAY 316606. The thought that skin POSTN + fibroblasts regulate and control diabetic foot skin injury re-healing through iron metabolism regulation and interaction of the skin POSTN + fibroblasts and endothelial cells is proved, a molecular mechanism is disclosed, and a powerful basis is provided for later research.

The present invention relates to a circadian rhythm regulation-based pH responsive anti-decline microsphere composition and application thereof, the circadian rhythm regulation-based pH responsive anti-decline microsphere composition comprises an inner sodium alginate microsphere and an outer chitosan coating layer, the inner sodium alginate microsphere encapsulates berberine and hyaluronic acid; the outer chitosan coating layer is used for coating nicotinamide, resveratrol and the inner sodium alginate microspheres. The pH response type anti-decline microsphere composition is applied to a skin care product. Compared with the prior art, the preparation method has the advantages that the circadian rhythm adaptive release of active ingredients is realized by constructing a pH response type double-layer microsphere structure of'sodium alginate on the inner layer and chitosan on the outer layer '.

The application discloses sulfhydrylated oxidized guar gum / sodium alginate microspheres and a preparation method and application thereof, and belongs to the field of biomedical materials. The sulfhydrylated oxidized guar gum and a sodium alginate base material are used to prepare and construct microspheres with pH response, colon targeting and intestinal adhesion by an emulsion gel method, the microsphere technology is used to embed probiotics, and the survival rate of the probiotics in gastric juice is improved. Through in-vitro verification, the method of embedding the microspheres successfully prevents the direct contact of the probiotics with a harsh environment, improves the ability of the probiotics to resist strong acid in the gastrointestinal tract, and enhances the adhesion and colonization effect of the probiotics in the intestinal tract. Through an animal experiment of simulating colitis, the LGG microspheres have the best treatment effect on colitis. Compared with a large number of side effects caused by antibiotic therapy, the treatment method has a certain clinical application prospect as a means with biological safety to treat inflammatory bowel disease.

The present application belongs to the technical field of biological medicine, and particularly relates to a sodium alginate microsphere co-loading nano-gold probiotics and curcumin albumin nanoparticles and preparation and application thereof. The present application synthesizes nano-gold by modifying probiotics Escherichia coli Nissle 1917, prepares curcumin albumin nanoparticles by using the principle of self-assembly, and then prepares the sodium alginate microsphere co-loading nano-gold probiotics and curcumin albumin nanoparticles by using the method of high-voltage electrostatic, which is used for the treatment of inflammatory bowel disease. Compared with the traditional preparation of nano-gold, the present application proposes a new green synthesis method, while keeping the activity of probiotics. In order to improve the problem of poor water solubility of curcumin, the present application uses albumin to load drugs, prepares curcumin albumin nanoparticles, and then combines with the sodium alginate microsphere, which has colon targeting, improves the bioavailability of probiotics and curcumin, and is effectively used for the treatment of inflammatory bowel disease.

The invention belongs to the technical field of microbial agents, and particularly relates to a charcoal-based nitrogen-fixing bacterium agent as well as a preparation method and application thereof. According to the invention, a biochar-humic acid-sodium alginate embedding mode is adopted, so that a porous protection cabin can be provided to adsorb plant rhizosphere nutrients. Meanwhile, a low-oxygen microcell is created to protect nitrogenase, physical protection is provided for thalli, and external environmental stress is buffered. Wherein humic acid is used as a high-quality organic carbon source and is beneficial to stimulating thalli to rapidly proliferate in embedded particles and rhizosphere, and a large number of active cells can be obtained in a short time. The biochar-based nitrogen-fixing bacterium agent (namely biochar-humic acid-sodium alginate microspheres) prepared by the invention has the advantages of simple and convenient preparation method, stable storage performance and the like, and compared with other biological agents, the biomass of the dried microbial agent reaches 2.2 * 10 < 10 > CFU / g, so that the biochar-based nitrogen-fixing bacterium agent has a good application prospect.

The invention relates to the technical field of biomedical materials, and discloses an injectable biocompatible hydrogel based on dynamic supramolecular assembly, and the hydrogel comprises the following components by weight: 3.0-5.0% of zinc oxide nanocrystalline regenerated cellulose; 3.0%-5.0% of chondroitin sulfate; 1.5% to 2.5% of recombinant human source collagen; 1%-2% of beta-sodium glycerophosphate; 0.7%-1% of a cyclodextrin polymer; 0.4%-0.7% of an adamantane derivative; 0.3% to 0.5% of PEI-DNA nano particles; 0.1% to 0.3% of calcium citrate; 0.1% to 0.2% of sodium alginate microspheres; 0.15%-0.2% of a pH buffer agent; the preparation method comprises the following steps: taking collagen as a raw material, taking sterile deionized water and normal saline as residual components, and adjusting the components through subject-object supramolecular interaction, ionic crosslinking, collagen thermosensitive assembly and a step-by-step mixing process and structure, so that the prepared hydrogel has the advantages of dynamic reversible crosslinking, shear thinning and self-healing synergy, high biocompatibility and controllable degradation rate.

The invention discloses an animal feather keratin extraction method which comprises the following steps: (1) mixing pretreated animal feathers with a tea saponin solution, and stirring to obtain degreased feathers; mixing the degreased feathers with an ethanol solution, and performing ultrasonic treatment to obtain loose feathers; (2) preparing liquid drops from the water phase and the oil phase through a micro-fluidic chip process, standing and curing the collected liquid drops, demulsifying and cleaning to obtain sodium alginate-CaCl2 microspheres; (3) mixing the loose feathers, water, the sodium alginate-CaCl2 microspheres and keratinase, then adjusting the pH value, and carrying out enzymolysis; finally, taking supernate; and (4) adjusting the pH value of the supernate, precipitating, dialyzing the precipitated mixed solution, and freeze-drying to obtain the animal feather keratin. According to the method disclosed by the invention, the slow release of calcium ions is realized by adding the sodium alginate-CaCl2 microspheres, so that the enzyme utilization rate is improved.

The present invention discloses a method for preparing sodium alginate / silica core-shell microspheres by a template method, and the method comprises the following steps: (1) Preparation of sodium alginate microspheres: By using a microsphere preparation instrument, sodium alginate microspheres of different sizes are prepared according to the required dimensions as the template cores for subsequent silica layer coating; (2) Coating of the silica shell layer: The sodium alginate microspheres in step (1) are placed in an aqueous solution containing tetraethoxysilane (TEOS), and a silica layer is in-situ polymerized on the surface of the sodium alginate microspheres through hydrolysis and self-condensation of TEOS to obtain sodium alginate / silica core-shell structure microspheres of different sizes. In the preparation process of this method, ethanol catalysis is not relied on, thereby weakening the condensation reaction between silanol groups and hydroxyl groups in sodium alginate. Through the hydrolysis of TEOS in the aqueous phase system and the self-condensation between silanol groups, a silica coating is formed and coated on the surface of the sodium alginate microspheres, and the controllable preparation of sodium alginate / silica core-shell microspheres of different sizes can be realized. In addition, the present invention also studies the influence of the size of the core-shell structure microspheres on their adsorption performance.

The invention discloses an inulin gel compound for nitric oxide delivery and application of the inulin gel compound in treatment of inflammatory bowel diseases, and the compound takes prebiotic inulin as a gel matrix and is loaded with zinc-cerium nano-enzyme and arginine-sodium alginate microspheres. A new strategy is provided for nitric oxide targeted delivery and comprehensive treatment of the inflammatory bowel disease, in the colon focus position, the system releases active components through a flora / pH dual response mechanism, zinc-cerium nano-enzyme catalyzes arginine to react to generate endogenous nitric oxide, excessive active oxygen is synchronously removed, and the endogenous nitric oxide can be effectively released. Further, targeted efficient delivery of nitric oxide is realized, intestinal inflammation is effectively relieved, and intestinal flora balance and intestinal barrier repair are promoted; meanwhile, the compound reduces the whole body and neuroinflammation level by inhibiting lipopolysaccharide from entering blood, so that anxiety and depression-like behaviors accompanied by the inflammatory bowel disease are improved.

The invention belongs to the field of orbit radiotherapy protection, and provides a biological response type conjunctival sac inner protector for orbit radiotherapy, which comprises a lens body, a central light-transmitting area is arranged in the middle of the lens body, a temporal upper shielding area is arranged on one side of the central light-transmitting area, a wearing arc area is arranged on the outer side of the central light-transmitting area, and the wearing arc area is arranged on the outer side of the central light-transmitting area. A side arc area is arranged on the outer side of the wearing arc area, a lower fornix medicine carrying area is arranged on the lower side of the central light-transmitting area, the vertical curvature radius of the lens body is 7.5 mm, the horizontal curvature radius of the lens body is 8.2 mm, and the curvature radius of the side arc area is larger than that of the wearing arc area; through the arrangement of the lens body with the central light-transmitting area, the upper temporal shielding area and the lower fornix drug-loading area, the damage of radiotherapy radiation to the sensitive area of the conjunctival sac can be accurately shielded by combining the directional shielding function of the 120-degree sector section of the upper temporal shielding area, and meanwhile, the drug-loading area integrated with the sodium alginate microspheres is utilized to realize continuous slow release of the drug, so that the curative effect of the drug-loading area is improved. And local tissue protection is enhanced.

The application discloses a selenium-rich spirulina culture method and belongs to the technical field of spirulina culture. The spirulina is solidified by using dopamine-modified sodium alginate microspheres containing magnetic particles, and is subjected to two-stage culture in low-selenium-concentration culture solution and high-selenium-concentration culture solution containing hexanoyl-L-homoserine lactone in sequence. The total selenium content and the organic selenium content of the obtained selenium-rich spirulina are high, and the organic selenium conversion rate is high.

The invention discloses a pH response type coating agent and application thereof in germination of puccinellia tenuifolia seeds. Comprising the following steps: pretreating puccinellia tenuifolia seeds with a mixed saline-alkali solution in a specific ratio, coating the puccinellia tenuifolia seeds with a double-layer coating agent consisting of a dimethylaminoethyl methacrylate-acrylic acid copolymer outer layer and a sodium alginate microsphere inner layer loaded with bacillus subtilis GB03, and culturing the puccinellia tenuifolia seeds under a variable-temperature condition of alternating illumination and darkness; when the pH value of the environment reaches a critical value, the coating agent is accurately swelled and broken to release viable bacteria, the activation effect of the saline-alkali solution is cooperated, the germination rate of the puccinellia tenuifolia seeds is remarkably increased, and the technical problem that the germination rate is low in saline-alkali soil vegetation reconstruction is effectively solved. The efficient germination method for the puccinellia tenuifolia is simple and easy to implement, does not need complex operation, and can quickly and effectively improve the germination rate of the puccinellia tenuifolia seeds. The method can be popularized and applied to production, so that the problem of low germination rate in the production process of the puccinellia sonchifolia is solved, and the method is of great significance to establishment of artificial grassland of the puccinellia sonchifolia.

The invention discloses probiotic freeze-dried powder and a preparation method of the probiotic freeze-dried powder, and the probiotic freeze-dried powder comprises the following raw materials in parts by weight: 1-10 parts of probiotics, 1-10 parts of probiotics and 1-10 parts of probiotics. The probiotic freeze-dried powder is prepared from the following raw materials in parts by weight: 100 to 300 parts of a compound probiotic agent, 30 to 50 parts of hydroxypropyl-beta-cyclodextrin, 20 to 40 parts of sodium alginate microspheres, 40 to 60 parts of galactooligosaccharide, 20 to 30 parts of resistant dextrin, 10 to 15 parts of cranberry concentrated juice, 5 to 10 parts of zinc gluconate, 3 to 8 parts of selenium-enriched yeast and 50 to 150 parts of a freeze-drying medium. According to the invention, the survival rate and the storage stability of thalli are remarkably improved through a composite strain compatibility-directional protective agent-low-temperature stepped freeze-drying synergistic technology; moreover, one-step elution is used for replacing multiple times of centrifugation, so that the energy consumption is obviously reduced.

The present application relates to the field of oocyte / embryo vitrification, and particularly relates to a method for preparing and vitrification of oocyte / embryo-loaded hydrogel microspheres. The present application first prepares oocyte-loaded cell microspheres encapsulated by sodium alginate hydrogel through a microfluidic chip, then places the collected cell-loaded microspheres in a freezing carrier, then places the freezing carrier loaded with the microspheres in a vitrification solution for CPA loading, and finally puts the freezing carrier loaded with the microspheres into liquid nitrogen for freezing preservation. The microfluidic chip used in the present application can stably generate oocyte-loaded sodium alginate microspheres with high dispersity and uniformity, the microsphere empty package rate and oocyte loss rate are low, and the survival rate and later development of the oocytes are good; the oocyte vitrification preservation technology can effectively reduce the concentration of the protective agent and the loading time, reduce the toxic effect and osmotic damage to the cells, and improve the survival rate and development rate of the oocytes.

The application provides a kind of targeted self-driven nanomotor hydrogel and its preparation method and application, belong to the field of biological medicine. Including the following steps: Janus mesoporous silica platinum nanomotor is synthesized by Pickering emulsion method, first loaded liraglutide by amination reaction, then grafted rapeseed DPP-IV inhibiting peptide by amide method, and encapsulated in sodium alginate microspheres, to obtain self-driven nanomotor hydrogel. The self-driven nanomotor described in the application can actively overcome the intestinal mucus barrier, pass through the mucus barrier into the specific site by autonomous navigation, significantly enhance the drug efficiency, avoid degradation in harsh stomach environment, reverse insulin resistance, reduce inflammatory complications such as diabetes, and enhance the safety and bioavailability of drugs.

The invention relates to the technical field of enzyme preparations, and particularly discloses toothpaste containing an enzyme preparation and a preparation method of the toothpaste, and the toothpaste comprises the following components in percentage by weight: 0.05%-0.2% of glucanase, 0.1%-0.3% of lysozyme, 0.02%-0.08% of lactoperoxidase, 3%-8% of nano-hydroxyapatite, 1%-3% of trehalose, 10%-20% of glycerol, 20%-40% of a friction agent, 1%-2% of a foaming agent and the balance of water. And the balance of deionized water. The glucanase, the lysozyme and the lactoperoxidase are respectively wrapped in the sodium alginate microspheres through a gradient pH embedding technology, the thickness of an embedding layer and the pH environment are controlled, external adverse factors are effectively isolated, and the storage stability and the use activity of the enzyme are remarkably improved.

The present invention discloses a method for cultivating anaerobic ammonia oxidation granular sludge with polydopamine-modified biochar loaded Fe-Cu sodium alginate microsphere inner core, comprising the following steps: biochar is modified with polydopamine, and then Fe-Cu ion loading is performed, and finally sodium alginate microspheres are prepared, and the prepared sodium alginate microspheres are used as inner cores, and co-cultured with activated Bacillus DMF-4 in an anaerobic environment. After continuous cultivation for 3 weeks, gravity sedimentation or centrifugation is used to collect, and anaerobic ammonia oxidation granular sludge with sodium alginate microsphere inner cores is obtained. Sodium alginate microspheres as inner cores provide greater density and good morphological support for sludge particles, avoid sludge swelling problems, and improve sedimentation performance. The sludge anti-shear performance is improved, and the iron ions that are slowly released and continuously released are enhanced, and the adsorption capacity of organic matter, ammonia nitrogen and heavy metals is enhanced, thereby improving the effect of sewage treatment. At the same time, it is a biodegradable material and will not cause secondary pollution during use.

The invention discloses a preparation method and application of an aquatic rahnella oral microcapsule sustained-release vaccine for fish, and relates to the field of fishery biological medicines. Sodium alginate and liquid paraffin which are two kinds of insoluble liquid are utilized, a stable emulsion is formed through a high-stirring-speed emulsifier, then the sodium alginate and calcium ions are physically cross-linked to be fixed, then a layer of chitosan coating is wrapped, and the rahnella aquatilis oral micro-capsule sustained-release vaccine is prepared, and the average particle size is 1-5 microns. The coating rate of Rahnella aquatica is measured by using a spectrophotometer after the Rahnella aquatica is subjected to decapsulation with decapsulation liquid, and the coating rate of thalli is 36.6%; the results of a zebra fish challenge experiment show that the protection rate of the vaccine is 92%, and the safety of the vaccine is 100%. In order to further clarify the efficacy of the vaccine, the detection of oral vaccine fish blood indexes and immune related genes such as TLR5, TNFa and uPAR has obvious up-regulated expression, compared with a non-immune group, the up-regulated expression in liver, head and kidney and muscle tissues is higher, and a related basis is provided for elaborating the pathogen and host PAMPs-PRRs process. The results show that the oral micro-capsule sustained-release vaccine has a good disease-resistant immune effect, and a foundation is laid and a thought is provided for development and application of fish oral vaccines. Therefore, the invention develops a novel sodium alginate microsphere oral sustained-release vaccine aiming at rahnella aquatica, and provides a new strategy for solving the problems of disease treatment and drug resistance of rahnella aquatica in aquaculture.

The application discloses a preparation method of polydopamine modified biochar loaded Fe-Cu sodium alginate microspheres, and biochar is prepared, the biochar is modified by polydopamine, the polydopamine modified biochar is loaded with Fe-Cu, and finally, the polydopamine modified biochar loaded Fe-Cu sodium alginate microspheres are obtained. The preparation method can be used for different particle sizes of sodium alginate microspheres, the prepared sodium alginate microspheres have the adsorption functions of ammonia nitrogen, nitrate nitrogen, chlorobenzene, phosphorus, chromium ions and lead ions, anaerobic ammonia oxidation granular sludge is prepared by taking the sodium alginate microspheres as a core, the sludge granule is provided with large density and good morphological support, sludge swelling problems are avoided, and the settling performance is improved; the adsorption capacity of the sludge to organic matters, ammonia nitrogen and heavy metals is enhanced, the effect of sewage treatment is improved, meanwhile, the sludge is a biodegradable material, and no secondary pollution is caused in the use process.

The application discloses a kind of frankincense taste jelly intelligent response type piglet teaching trough compound feed and preparation method, it is related to animal feed technical field, for the problems such as poor palatability of traditional feed, mismatch of nutrient release and physiological demand, high-temperature process destroys heat-sensitive component, the present application is realized accurate release of nutrition by triple signal response system and jelly matrix collaborative design.The feed includes energy raw material, protein raw material, intelligent response component (pH sensitive sodium alginate microsphere load sodium butyrate / glutamine, temperature-sensitive xanthan gum-glucomannan compound gel wrapped antibacterial peptide, enzyme-sensitive lysine-lysine peptide bond crosslinking layer embedded glucose complex enzyme), flavor system (vanillin microcapsule) and konjac gum jelly matrix.pH-temperature-enzyme triple response mechanism, adapt to piglet gastric acid gradient, body temperature fluctuation and pancreatic enzyme activity;Frankincense slow-release and nutrient release space-time cooperation, feeding frequency is increased by 40%;Konjac gum matrix reduces dust, gel strength is ≥750g / cm².