Microbial amino acid-containing water-soluble fertilizer and preparation method thereof

Abstract

The invention relates to the technical field of fertilizers, in particular to a microbial amino acid-containing water-soluble fertilizer and a preparation method thereof. The method comprises the following steps: providing a first corn steep liquor and a second corn steep liquor; adding a Trichoderma harzianum-Purpureocillium lilacinum complex microbial inoculant and lactic acid bacteria into the first corn steep liquor, and carrying out first fermentation to obtain a first fermentation broth; mixing the first fermentation liquor with the second corn steep liquor to obtain mixed corn steep liquor; adding bacillus subtilis into the mixed corn steep liquor, and carrying out second fermentation to obtain second fermentation liquor; and sequentially adding a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer into the second fermentation liquor to obtain the water-soluble fertilizer containing microbial amino acids. The microbial amino acid-containing water-soluble fertilizer prepared by the preparation method provided by the invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility, promote the root development of the crops and improve the resistance of the crops to adversity.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and particularly relates to a microbial amino acid water-soluble fertilizer and a preparation method thereof. Background Art

[0002] In the field of modern agricultural technology, the research on the efficient utilization of natural resources and the improvement of crop growth quality is becoming increasingly in-depth. Among them, corn, as a widely planted food crop, is not only an important source of human food, but its by-products also contain rich bioactive ingredients and nutrients, providing valuable secondary resources for agricultural production. Various processing wastes directly derived from the raw material origin of corn, such as corn steep liquor, are regarded as a natural treasure trove for improving soil fertility and crop stress resistance because they contain a large amount of amino acids, vitamins, minerals, and various bioactive substances.

[0003] Corn steep liquor, as a by-product in the corn wet milling process, contains water-insoluble substances such as corn silk and corn husk. These components are often overlooked, but in fact, they are rich in dietary fiber, polyphenolic compounds, and unused starch and protein, and have extremely high nutritional value. Traditional treatment methods often neglect the in-depth development of these valuable resources, resulting in resource waste. In recent years, with the progress of biotechnology, by adopting advanced processing technologies, such as treatment with a pipeline high-shear emulsifier, not only can the cell wall structure in corn steep liquor be effectively broken to release more intracellular nutrients, but also impurities can be removed through fine filtration, laying a good foundation for the subsequent biological fermentation process.

[0004] Microbial fermentation technology, as an environmentally friendly and efficient conversion means, can convert complex organic substances in corn steep liquor into organic nutrients easily absorbed and utilized by crops, such as amino acids, organic acids, vitamins, etc. These substances have significant effects on promoting crop growth and enhancing crop immunity. However, the selection and optimization of the fermentation process are directly related to the quality and efficiency of the final product. Adding all the corn steep liquor to the fermentation system at one time for fermentation, although the operation is simple, often leads to low fermentation efficiency and uneven product quality due to problems such as too high substrate concentration and uneven release of nutrient components. Summary of the Invention

[0005] To solve the deficiencies in the above-mentioned prior art, the present invention provides a microbial amino acid water-soluble fertilizer and a preparation method thereof;

[0006] To solve the above technical problems, one of the technical solutions provided by the present invention is as follows:

[0007] A preparation method of a microbial amino acid water-soluble fertilizer, comprising the following steps:

[0008] Provide a first corn steep liquor and a second corn steep liquor;

[0009] Add Trichoderma harzianum and Purpureocillium lilacinum complex microbial agent and lactic acid bacteria to the first corn steep liquor for the first fermentation to obtain a first fermentation broth;

[0010] Mix the first fermentation broth and the second corn steep liquor to obtain a mixed corn steep liquor;

[0011] Add Bacillus subtilis to the mixed corn steep liquor for the second fermentation to obtain a second fermentation broth;

[0012] Add nitrogen fertilizer, phosphate fertilizer and potassium fertilizer to the second fermentation broth in sequence to obtain a microbial amino acid water-soluble fertilizer.

[0013] In one embodiment, the mass ratio of the first corn steep liquor to the second corn steep liquor is 1:1.

[0014] In one embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the Trichoderma harzianum and Purpureocillium lilacinum complex microbial agent is 1000:1; and / or

[0015] The mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the lactic acid bacteria is 1800:1.

[0016] In one embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the Bacillus subtilis is 200:1.

[0017] In one embodiment, the temperature of the first fermentation is 20 - 30 °C, and the time of the first fermentation is 7 days; and / or

[0018] The temperature of the second fermentation is 25 - 30 °C, and the time of the second fermentation is 7 days.

[0019] In one embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is (25:4) - (10:1);

[0020] Preferably, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is 25:4;

[0021] Preferably, the nitrogen fertilizer is selected from any one or a combination of at least two of nitrate nitrogen, ammonium nitrogen, and amide nitrogen.

[0022] In one embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is (20:1) - (50:1);

[0023] Preferably, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is 20:1;

[0024] Preferably, the phosphate fertilizer is selected from any one or a combination of at least two of monoammonium phosphate, diammonium phosphate, and potassium dihydrogen phosphate.

[0025] In one embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the potassium fertilizer is 100:3;

[0026] Preferably, the potassium fertilizer is selected from any one or a combination of at least two of potassium chloride, potassium sulfate, and potassium nitrate.

[0027] In one embodiment, after the nitrogen fertilizer is added, at least 2 hours elapse before adding the phosphate fertilizer and the potassium fertilizer in sequence.

[0028] The second technical solution provided by the present invention is as follows:

[0029] A microbial amino acid water-soluble fertilizer prepared by the method as described above.

[0030] Based on the above, compared with the prior art, the microbial amino acid water-soluble fertilizer prepared by the preparation method provided by the present invention can effectively promote the growth of soybeans, improve the quality of soybeans, increase the yield and its components, and increase soil fertility, and improve the resistance of crops to adversity.

[0031] Other features and beneficial effects of the present invention will be described in the subsequent description, and, in part, will be obvious from the description or will be understood by implementing the present invention. The objectives and other beneficial effects of the present invention can be achieved and obtained through the structures specifically pointed out in the description and claims. Detailed Embodiments

[0032] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments; the technical features designed in different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other; based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0033] In the description of the present invention, it should be noted that all terms used in the present invention (including technical terms and scientific terms) have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains, and should not be construed as limiting the present invention; it should be further understood that the terms used in the present invention should be understood as having a meaning consistent with their meaning in the context of this specification and the relevant art, and should not be understood in an idealized or overly formal sense, unless clearly defined as such in the present invention.

[0034] An embodiment of the present invention provides a preparation method of a microbial amino acid water-soluble fertilizer, comprising the following steps:

[0035] Step 1: Provide a first corn steep liquor and a second corn steep liquor;

[0036] In a preferred embodiment, the mass ratio of the first corn steep liquor to the second corn steep liquor is 1:1. This is because corn steep liquor is rich in nutrients such as protein, amino acids, reducing sugars, and growth factors. Adding corn steep liquor in stages with a mass ratio of 1:1 can ensure the continuous supply of nutrients in the fermentation system and avoid nutrient surplus or deficiency caused by a one-time addition. This ratio helps microorganisms obtain suitable nutrients at different fermentation stages and promotes the coordinated progress of their metabolic activities. At the same time, the acidity and composition of corn steep liquor have a significant impact on the fermentation environment. By adding in stages, the pH value and nutrient concentration of the fermentation broth can be better controlled. For example, the metabolites produced in the first fermentation (such as lactic acid) can adjust the acidity of the fermentation environment and create more suitable conditions for the second fermentation. This way of adding in stages helps maintain the stability of the fermentation system and the activity of microorganisms. Moreover, adding corn steep liquor in stages can avoid the catabolic repression effect caused by a one-time high-concentration fermentation. In the first fermentation, microorganisms can utilize the nutrients in corn steep liquor for growth and metabolism to produce beneficial intermediate products. After adding the second corn steep liquor, these intermediate products can be further transformed and optimized, thereby improving the overall quality of the fermentation products. During the fermentation process, different microorganisms (such as Trichoderma harzianum, Lactobacillus, and Bacillus subtilis) have different metabolic characteristics. Adding corn steep liquor in stages can provide a more suitable growth environment for these microorganisms, promote their synergistic effects, and thus improve the fermentation efficiency and the biological activity of the products.

[0037] Step 2: Add a Trichoderma harzianum - Purpureocillium lilacinum complex bactericide and Lactobacillus to the first corn steep liquor for the first fermentation to obtain a first fermentation broth;

[0038] In this step, the corn steep liquor contains rich nutrients such as carbohydrates, proteins, and minerals. Trichoderma harzianum and Purpureocillium lilacinum can secrete extracellular enzymes to decompose complex organic substances in the corn steep liquor and convert them into simple nutrients that are more easily utilized by other microorganisms. Lactobacillus ferments to produce lactic acid, reducing the pH value of the fermentation environment and creating an acidic condition for subsequent fermentation. Moreover, Trichoderma harzianum and Purpureocillium lilacinum have broad-spectrum biocontrol capabilities and can inhibit the growth and reproduction of pathogenic bacteria. They can reduce the living space of harmful bacteria through nutrient competition, hyperparasitism, etc. In addition, their fermentation products also have antibacterial activity and can inhibit pathogenic bacteria in the soil and prevent soil-borne diseases. These strains will produce some plant growth promoting factors during the fermentation process, such as bioenzymes and active substances, which can stimulate the growth of plant roots and improve the nutrient absorption efficiency of plants.

[0039] In a preferred embodiment, the temperature of the first fermentation is 20 - 30 °C, and the time of the first fermentation is 7 days;

[0040] In some preferred embodiments, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the Trichoderma harzianum - Purpureocillium lilacinum complex microbial agent is 1000:1; and / or the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the lactic acid bacteria is 1800:1.

[0041] Step 3: Mix the first fermentation broth and the second corn steep liquor to obtain a mixed corn steep liquor;

[0042] In the mixing process of this step, first, for the fermentation process, corn steep liquor is rich in nutrients such as soluble proteins, sugars, and auxins. The corn steep liquor after the first fermentation has been partially transformed, and the nutrients have been preliminarily decomposed, while the unfermented corn steep liquor added for the second time provides new nutrients. This mixing method can provide a more balanced carbon source and nitrogen source for microorganisms, avoiding nutrient deficiency or excess, thus maintaining the stability and efficiency of the fermentation process; at the same time, during the first fermentation process, microorganisms such as lactic acid bacteria will convert the sugars in the corn steep liquor into lactic acid, reducing the pH value of the fermentation broth. This acidic environment is beneficial to the growth of Trichoderma harzianum and Purpureocillium lilacinum, while inhibiting the reproduction of harmful bacteria. After adding the new unfermented corn steep liquor, the pH value of the fermentation broth can be further adjusted to make it more suitable for the growth of subsequent microorganisms; and because Trichoderma harzianum and Purpureocillium lilacinum will produce various bioactive substances during the fermentation process, such as antibacterial components and enzymes. Mixed fermentation can extend the fermentation time and increase the accumulation of these active substances, thereby improving the biological control effect of the fermentation product; second, for microorganisms, Trichoderma harzianum and Purpureocillium lilacinum require suitable nutrients and environmental conditions during the fermentation process. Mixed fermentation can provide richer nutrients, and at the same time, by adjusting the pH value of the fermentation broth, it is made more suitable for the growth and metabolism of these two kinds of bacteria. In addition, the fermentation broth of Trichoderma harzianum has been proven to improve the structure of the bacterial community in the soil and promote the growth of other beneficial bacteria; and because lactic acid bacteria have already converted part of the sugars into lactic acid during the first fermentation, reducing the pH value of the fermentation broth. After adding the new corn steep liquor, lactic acid bacteria can obtain more sugars as a carbon source, further enhancing their metabolic activity and producing more lactic acid and other metabolites; in addition, Trichoderma harzianum, Purpureocillium lilacinum, and lactic acid bacteria have a synergistic effect during the fermentation process. Mixed fermentation can promote the interaction between these microorganisms and optimize the types and contents of metabolites. For example, Trichoderma harzianum can decompose complex organic substances to provide more easily utilized sugars for lactic acid bacteria, and the acidic environment produced by lactic acid bacteria is beneficial to the growth of Trichoderma harzianum.

[0043] Step 4: Add Bacillus subtilis to the mixed corn slurry for secondary fermentation to obtain a secondary fermentation broth;

[0044] Further adding unfermented secondary corn slurry can further optimize the fermentation environment. Since Bacillus subtilis is an aerobic bacterium that can grow and reproduce in an acidic environment. Based on the first-stage fermentation, Bacillus subtilis can utilize the remaining nutrients in the corn slurry to further optimize the composition of the fermentation products. Moreover, Bacillus subtilis will produce spores during the fermentation process. The spores have characteristics such as high temperature resistance and dry resistance, which can significantly improve the stability and shelf life of the fermentation products. Not only that, Bacillus subtilis can produce a variety of antibacterial substances and has an inhibitory effect on a variety of pathogenic bacteria. Its synergistic effect with Trichoderma harzianum and Purpureocillium lilacinum can more effectively inhibit pathogenic bacteria and enhance the overall biological control effect.

[0045] In a preferred embodiment, the temperature of the secondary fermentation is 25 - 30 °C, and the time of the secondary fermentation is 7 days;

[0046] In a preferred embodiment, the mass ratio of the sum of the first corn slurry and the second corn slurry to Bacillus subtilis is 200:1.

[0047] Step 5: Add nitrogen fertilizer, phosphate fertilizer, and potassium fertilizer to the secondary fermentation broth in sequence to obtain a microbial amino acid water-soluble fertilizer.

[0048] Specifically, nitrogen fertilizer can promote the growth of plant branches and leaves, increase the chlorophyll content, improve the photosynthesis efficiency of plants, make plants lush, and improve quality indicators such as the protein content of agricultural products; phosphate fertilizer plays an important role in plant root development, flowering, and fruiting, can promote plants to flower and fruit earlier, improve the quality and yield of fruits, and enhance the stress resistance of plants, such as drought resistance and cold resistance; potassium fertilizer helps plant stems to be strong, enhances the lodging resistance of plants, promotes the absorption and transformation of nutrients such as nitrogen and phosphorus by plants, improves the taste, color, etc. of agricultural products, and also enhances the disease resistance of plants; when cooperating with the microorganisms in the fermentation broth, the microorganisms can decompose organic substances in the soil and release more nutrients for plants to absorb. At the same time, nitrogen, phosphate, and potassium fertilizers provide additional nutrition for the growth and reproduction of microorganisms, promote the activity of microorganisms, and enable them to better play roles such as improving the soil and inhibiting harmful microorganisms; at the same time, the amino acid water-soluble fertilizer is rich in various nutrients, can promote the root development of crops, enhance photosynthesis, improve the chlorophyll content and enzyme activity of crops, thereby increasing crop yields. After adding nitrogen, phosphate, and potassium fertilizers, the amino acids in the formed microbial amino acid water-soluble fertilizer can form complexes with nitrogen, phosphorus, and potassium, reducing the volatilization and loss of nutrients and further improving the fertilizer utilization rate.

[0049] In a preferred embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is (25:4)-(10:1);

[0050] Preferably, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is 25:4;

[0051] Preferably, the nitrogen fertilizer is selected from any one or a combination of at least two of nitrate nitrogen, ammonium nitrogen, and amide nitrogen.

[0052] More preferably, after the nitrogen fertilizer is added, at least 2 hours should elapse before adding the phosphate fertilizer and potassium fertilizer in sequence.

[0053] One of the purposes of this operation is to avoid nutrient antagonism. For example, nitrogen-phosphorus antagonism: a large amount of ammonium nitrogen will affect the plant's absorption of phosphorus. If a large amount of nitrogen fertilizer and phosphate fertilizer are added simultaneously, it may cause ammonium ions in the soil to combine with phosphate ions to form insoluble ammonium phosphate salts, reducing the effectiveness of phosphorus and affecting the plant's absorption of phosphorus. Adding at intervals can reduce this antagonistic effect and enable phosphorus to be better absorbed and utilized by plants; another example is nitrogen-potassium antagonism: excessive potassium ions will inhibit the plant's absorption of ammonium ions, and at the same time, a high concentration of ammonium nitrogen will also affect the absorption of potassium ions. Adding nitrogen fertilizer first and allowing the plant to have a certain time to absorb and utilize part of the nitrogen before adding potassium fertilizer can reduce the antagonistic effect between the two and ensure the normal absorption of nitrogen and potassium by the plant;

[0054] Moreover, microorganisms have different nutrient requirements at different stages. Adding nitrogen fertilizer first can provide the nitrogen source required for the growth and reproduction of microorganisms, promoting their massive proliferation and metabolic activities. After a period of time, the metabolic process of microorganisms will change the soil environment. At this time, adding phosphate fertilizer and potassium fertilizer is more conducive to microorganisms using these nutrients for further metabolism and the transformation of soil nutrients.

[0055] During the plant growth process, the requirements for nitrogen, phosphorus, and potassium have a sequential order and different proportions. Generally, the demand for nitrogen is relatively large in the early growth stage to promote the growth of branches and leaves. As the growth process progresses, the demand for phosphorus and potassium gradually increases for root development, flowering, and fruiting. Adding at intervals in the order of nitrogen first and then phosphorus and potassium is more in line with the law of phased nutrient requirements during plant growth, can improve the utilization rate of fertilizers, and promote the healthy growth of plants;

[0056] In addition, ammonium nitrogen or urea in nitrogen fertilizer needs a certain time to be converted in the soil. For example, urea needs to be converted into ammonium nitrogen under the action of urease to be better absorbed by plants and adsorbed and fixed by the soil. Adding nitrogen fertilizer first and leaving an interval is conducive to the preliminary conversion and stabilization of nitrogen fertilizer in the soil. After that, adding phosphate fertilizer and potassium fertilizer can enable the three fertilizers to be gradually released and utilized in the soil, reducing nutrient loss and volatilization, and improving the overall effectiveness and utilization rate of fertilizers.

[0057] In a preferred embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is (20:1)-(50:1);

[0058] Preferably, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is 20:1;

[0059] Preferably, the phosphate fertilizer is selected from any one or a combination of at least two of monoammonium phosphate, diammonium phosphate, and potassium dihydrogen phosphate.

[0060] In a preferred embodiment, the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the potassium fertilizer is 100:3;

[0061] Preferably, the potassium fertilizer is selected from any one or a combination of at least two of potassium chloride, potassium sulfate, and potassium nitrate.

[0062] Step Six: Fill the prepared microbial amino acid water-soluble fertilizer into barrels, and then place it at room temperature of 15-30 °C for at least 3 days before use.

[0063] In this step, since the microbial amino acid water-soluble fertilizer contains various beneficial microorganisms (such as Trichoderma harzianum, lactic acid bacteria, etc.), these microorganisms are already in an active state during the fermentation process. Placing it at room temperature of 15-30 °C for a period of time can allow the microorganisms to gradually adapt to the new environment and reduce the activity fluctuations caused by environmental changes such as temperature and humidity. In addition, this temperature range is also beneficial for the further stabilization of the metabolites of the microorganisms, avoiding unstable fertilizer efficiency due to overly active microorganisms during use. Moreover, the amino acids, nitrogen, phosphorus, potassium and other components in the amino acid water-soluble fertilizer may not have reached a completely stable state during the preparation process. Placing it at room temperature of 15-30 °C for 3 days can allow these components to further combine and stabilize, forming a more stable complex or chelate state, thereby improving the stability and absorption efficiency of the fertilizer. Not only that, during the preparation process, the metabolic activities of the microorganisms may cause gas generation, resulting in the phenomenon of barrel swelling. Placing it at room temperature for a period of time can gradually weaken the metabolic activities of the microorganisms and complete the gas release, thereby reducing the risk of barrel swelling. This treatment method has been proven in practical applications to significantly improve the storage stability of the fertilizer. At the same time, after placing it at room temperature for a period of time, the microorganisms and nutrient components in the fertilizer will reach a relatively balanced state. This balanced state is beneficial for improving the fertilizer efficiency of the fertilizer and avoiding poor fertilizer efficiency or uneven nutrient release caused by insufficiently stable components; finally, the amino acid water-soluble fertilizer may have a certain fermentation smell after preparation, and placing it at room temperature can gradually weaken these peculiar smells. At the same time, the placement process also helps to reduce the loss of volatile components in the fertilizer, thereby improving the utilization rate of the fertilizer.

[0064] During the early-stage experiments of the present invention, corn steep liquor was centrally collected and subjected to a one-time overall fermentation treatment in order to obtain organic fertilizers that can be used in agriculture. However, the experimental results showed that the method of adding all the corn steep liquor at once for fermentation was not ideal. On the one hand, due to the extremely high sugar and other organic matter content in the corn steep liquor, a large amount of input into the fermentation system at one time easily led to difficult control of the fermentation process, low fermentation efficiency, and even the production of adverse fermentation products such as alcohol and acetic acid, which are harmful to crop growth. On the other hand, one-time fermentation was difficult to fully convert all the nutrients in the corn steep liquor, resulting in a significant reduction in the fertilizer efficiency of the final product and still low absorption efficiency of the crop for the fertilizer.

[0065] In contrast, adopting a strategy of adding corn steep liquor in stages and fermenting with different strains showed more superior performance. Specifically, first, a part of the corn steep liquor was added to the fermentation system and inoculated with specific starting strains, which could quickly adapt to the environment and start preliminary fermentation, decomposing some complex organic substances and creating more favorable conditions for the subsequent fermentation process. Subsequently, when the fermentation reached a certain degree, the remaining corn steep liquor was added, and another type of strain that could utilize the products of the previous stage was introduced for relay fermentation. This way of stepwise addition and strain relay not only effectively reduced the substrate inhibition effect, improved the fermentation efficiency, but also promoted the synergy between different strains, maximizing the conversion of nutrients in the corn steep liquor to produce more diversified and higher-quality organic fertilizers or biostimulants.

[0066] The advantage of the stepwise addition strategy is that it can effectively control the fermentation process, avoid fermentation out of control and nutrient loss caused by a large amount of addition at one time, and enable the organic substances in the corn steep liquor to be more fully and evenly converted into nutrient components that are easy for crops to absorb. In addition, after fermentation, according to the growth requirements of crops, inorganic fertilizer components such as N, P, and K were added to make a microbial amino acid water-soluble fertilizer. This fertilizer not only greatly improved the utilization rate of corn steep liquor, achieved high-value utilization of waste, reduced environmental pollution, but also significantly reduced the production cost of enterprises.

[0067] In practical applications, this new type of water-soluble fertilizer demonstrated excellent fertilizer efficiency. It can effectively increase the yield and quality of crops, improve soil structure, enhance soil fertility, promote the development of crop roots, and improve the resistance of crops to adversity. At the same time, due to its easy solubility and absorption characteristics, nutrient loss was reduced, and the fertilizer utilization rate was improved, thus bringing significant economic and environmental benefits to agricultural production.

[0068] The beneficial effects of this application will be described below in combination with specific examples and comparative examples.

[0069] Example 1

[0070] This embodiment provides a preparation method of a microbial amino acid water-soluble fertilizer, comprising the following steps:

[0071] Step 1: Provide a first corn steep liquor and a second corn steep liquor, and the mass ratio of the first corn steep liquor to the second corn steep liquor is 1:1. In this embodiment, the first corn steep liquor is 5000 kg, and the second corn steep liquor is 5000 kg;

[0072] Step 2: Add a Trichoderma harzianum - Purpureocillium lilacinum complex bactericide and lactic acid bacteria to the first corn steep liquor for the first fermentation to obtain a first fermentation broth; the temperature of the first fermentation is 20 - 30 °C, and the time of the first fermentation is 7 days; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the Trichoderma harzianum - Purpureocillium lilacinum complex bactericide is 1000:1; the Trichoderma harzianum - Purpureocillium lilacinum complex bactericide uses Trichoderma harzianum - Purpureocillium lilacinum (produced by Laoshi Yinuo, with the effective viable count ≥ 500 million / g); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the lactic acid bacteria is 1800:1.

[0073] Step 3: Mix the first fermentation broth and the second corn steep liquor, and stir for 20 min to obtain a mixed corn steep liquor.

[0074] Step 4: Add Bacillus subtilis to the mixed corn steep liquor for the second fermentation to obtain a second fermentation broth; the temperature of the second fermentation is 25 - 30 °C, and the time of the second fermentation is 7 days; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to Bacillus subtilis is 200:1; Bacillus subtilis is used (produced by Shandong Weilan Biotechnology Co., Ltd.).

[0075] Step 5: Sequentially add nitrogen fertilizer, phosphate fertilizer, and potassium fertilizer to the second fermentation broth to obtain a microbial amino acid water-soluble fertilizer. The phosphate fertilizer and potassium fertilizer are added 2 h after the addition of the nitrogen fertilizer; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is 10:1; the nitrogen fertilizer uses a water-soluble polymorphic nitrogen fertilizer (produced by Xinjiang Yuxiang Poplar Chemical Co., Ltd., N - P2O5 - K2O: 40 - 0 - 0); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is 50:1; the phosphate fertilizer uses monoammonium phosphate (produced by Yuntu New Energy Materials (Jingzhou) Co., Ltd., N - P2O5 - K2O: 12 - 61 - 0); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the potassium fertilizer is 100:3; the potassium fertilizer uses fully water-soluble agricultural potassium sulfate (produced by Guotou Xinjiang Lop Nur Potash Co., Ltd., with the water-soluble potassium oxide (K2O) ≥ 53.8%).

[0076] Step 6: Fill the prepared microbial amino acid water-soluble fertilizer into barrels, and then place it at room temperature of 25 °C for 3 days.

[0077] Example 2

[0078] This embodiment provides a preparation method of a microbial amino acid water-soluble fertilizer, which includes the following steps:

[0079] Step 1: Provide a first corn steep liquor and a second corn steep liquor, and the mass ratio of the first corn steep liquor to the second corn steep liquor is 1:1. In this embodiment, the first corn steep liquor is 5000 kg and the second corn steep liquor is 5000 kg;

[0080] Step 2: Add a Trichoderma harzianum - Purpureocillium lilacinum complex microbial agent and lactic acid bacteria to the first corn steep liquor for the first fermentation to obtain a first fermentation broth; the temperature of the first fermentation is 20 - 30 °C, and the time of the first fermentation is 7 days; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the Trichoderma harzianum - Purpureocillium lilacinum complex microbial agent is 1000:1; the Trichoderma harzianum - Purpureocillium lilacinum complex microbial agent uses Trichoderma harzianum - Purpureocillium lilacinum (produced by Laoshi Yinuo, with an effective viable count of ≥ 500 million / g); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the lactic acid bacteria is 1800:1.

[0081] Step 3: Mix the first fermentation broth and the second corn steep liquor, and stir for 20 min to obtain a mixed corn steep liquor.

[0082] Step 4: Add Bacillus subtilis to the mixed corn steep liquor for the second fermentation to obtain a second fermentation broth; the temperature of the second fermentation is 25 - 30 °C, and the time of the second fermentation is 7 days; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to Bacillus subtilis is 200:1; Bacillus subtilis is used (produced by Shandong Weilan Biotechnology Co., Ltd.).

[0083] Step 5: Sequentially add nitrogen fertilizer, phosphate fertilizer, and potassium fertilizer to the second fermentation broth to obtain a microbial amino acid water-soluble fertilizer. The phosphate fertilizer and potassium fertilizer are added 2 h after the addition of the nitrogen fertilizer; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is 50:7; the nitrogen fertilizer uses a water-soluble polymorphic nitrogen fertilizer (produced by Xinjiang Yuxiang Poplar Chemical Co., Ltd., N-P2O5-K2O: 40-0-0); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is 100:3; the phosphate fertilizer uses monoammonium phosphate (produced by Yuntu New Energy Materials (Jingzhou) Co., Ltd., N-P2O5-K2O: 12-61-0); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the potassium fertilizer is 100:3; the potassium fertilizer uses fully water-soluble agricultural potassium sulfate (produced by Guotou Xinjiang Lop Nur Potash Co., Ltd., with water-soluble potassium oxide (K2O) ≥ 53.8%).

[0084] Step 6: Fill the prepared microbial amino acid water-soluble fertilizer into barrels and then place them at room temperature of 25°C for 3 days.

[0085] Example 3

[0086] This embodiment provides a preparation method of a microbial amino acid water-soluble fertilizer, including the following steps:

[0087] Step 1: Provide a first corn steep liquor and a second corn steep liquor, and the mass ratio of the first corn steep liquor to the second corn steep liquor is 1:1. In this embodiment, the first corn steep liquor is 5000 kg and the second corn steep liquor is 5000 kg.

[0088] Step 2: Add a Trichoderma harzianum - Purpureocillium lilacinum complex bactericide and lactic acid bacteria to the first corn steep liquor for the first fermentation to obtain a first fermentation broth; the temperature of the first fermentation is 20 - 30°C, and the time of the first fermentation is 7 days; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the Trichoderma harzianum - Purpureocillium lilacinum complex bactericide is 1000:1; the Trichoderma harzianum - Purpureocillium lilacinum complex bactericide uses Trichoderma harzianum - Purpureocillium lilacinum (produced by Laoshi Yinuo, with an effective viable count ≥ 500 million / g); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the lactic acid bacteria is 1800:1.

[0089] Step 3: Mix the first fermentation broth and the second corn steep liquor and stir for 20 min to obtain a mixed corn steep liquor.

[0090] Step 4: Add Bacillus subtilis to the mixed corn steep liquor for the second fermentation to obtain a second fermentation broth; the temperature of the second fermentation is 25 - 30°C, and the time of the second fermentation is 7 days; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to Bacillus subtilis is 200:1; Bacillus subtilis is used (produced by Shandong Weilan Biotechnology Co., Ltd.).

[0091] Step 5: Add nitrogen fertilizer, phosphate fertilizer, and potassium fertilizer to the second fermentation broth in sequence to obtain a microbial amino acid water-soluble fertilizer. The phosphate fertilizer and potassium fertilizer are added 2 hours after the addition of the nitrogen fertilizer; the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is 50:7; the nitrogen fertilizer used is a water-soluble polymorphic nitrogen fertilizer (produced by Xinjiang Yuxiang Huayang Chemical Co., Ltd., N-P2O5-K2O: 40-0-0); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is 100:3; the phosphate fertilizer used is monoammonium phosphate (produced by Yuntu New Energy Materials (Jingzhou) Co., Ltd., N-P2O5-K2O: 12-61-0); the mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the potassium fertilizer is 100:3; the potassium fertilizer used is a fully water-soluble agricultural potassium sulfate (produced by SDIC Xinjiang Lop Nur Potash Co., Ltd., water-soluble potassium oxide (K2O) ≥ 53.8%).

[0092] Step 6: Fill the prepared microbial amino acid water-soluble fertilizer into barrels and then place them at room temperature of 25°C for 3 days.

[0093] Comparative Example 1 :

[0094] The difference between this comparative example and Example 3 is that the first corn steep liquor and the second corn steep liquor are mixed, and a compound microbial agent of Trichoderma harzianum and Purpureocillium lilacinum, lactic acid bacteria, and Bacillus subtilis are added thereto.

[0095] Test Example

[0096] (1) Determination of soybean agronomic traits, yield, and yield components

[0097] At the soybean maturity stage, in each plot (the plot area is 666.7 m 2 , with 18,000 plants per mu and machine sowing; for all treatments, 5000 ml / mu is root-applied with water each time at the soybean seedling stage, full-bloom stage, and pod-filling stage, and 150 ml / mu is foliar-sprayed, and other management conditions are the same as those of conventional cultivation), 10 plants with uniform growth and representativeness are selected in each plot, dug out as a whole plant, tied with labels, and taken back to the laboratory to measure agronomic traits, yield components, quality, and soil fertility. The agronomic traits such as plant height, bottom pod height, stem diameter, and effective branch number are measured with a tape measure and a vernier caliper, the grain weight per plant and 100-seed weight are measured with an electronic scale, and the fat content is measured by the first method of GB 5009.6-2016.

[0098] Table 1 Effects on soybean growth

[0099]

[0100]

[0101] Compared with the comparative example, the examples can significantly increase the stem diameter of soybeans, increase the number of nodes on the main stem of soybeans, and increase the number of effective branches; among them, the stem diameter of Example 3 is the largest, 1.54 cm higher than that of the control, and the number of nodes on the main stem and the number of effective branches of Example 2 are the highest, increasing by 7.04% and 75% respectively compared with the control.

[0102] Table 2 Effects on the quality, yield and component factors of soybeans

[0103] Number of pods per plant (pcs) Low pod height (cm) Number of grains per plant (pcs) Number of grains per pod (pcs) Example 1 36.20±6.73b 27.00±8.12ab 81.50±21.73bc 2.23±0.27a Example 2 52.40±7.71a 26.00±4.47ab 130.10±29.95a 2.47±0.31a Example 3 48.80±10.74a 25.40±6.04ab 112.40±28.28ab 2.30±0.14a Comparative Example 1 23.25±5.75c 24.21±9.24b 60.25±15.51c 2.32±0.28a Grain weight per plant (g) 100-grain weight (g) Yield per mu (kg) Fat (g / 100g) Example 1 13.46±4.05b 17.64±0.72b 205.94±61.93b 15.47±0.42a Example 2 24.48±6.25a 19.50±0.16a 374.56±95.57a 13.47±0.15bc Example 3 17.76±4.72b 17.37±0.31bc 271.65±72.29b 13.80±0.10b Comparative Example 1 8.11±2.14c 16.99±0.20c 123.8±45.92c 13.42±0.12c

[0104] Compared with the comparative example, the examples can significantly increase the number of pods per plant, the grain weight per plant, the yield per mu in terms of conversion, etc. of soybeans; among them, the number of pods per plant, the grain weight per plant, the yield per mu in terms of conversion and the number of grains per plant of Example 2 are the highest, increasing by 29.15, 16.37 g, 250.76 kg and 69.85 respectively compared with Comparative Example 1.

[0105] (2) Determination of soil nutrient content

[0106] Before winter plowing after soybean harvest, soil samples of 0-40 cm soil layer were collected from each treatment to determine soil fertility by using the five-point sampling method with reference to the methods of Ma Mingze et al. The soil organic matter content was determined by the external heating method with potassium dichromate, the total nitrogen was determined by the semi-micro Kjeldahl method, the total potassium was determined by the sodium hydroxide melting method, the available phosphorus content was extracted by sodium bicarbonate and determined by the molybdenum-antimony anti-colorimetric method, the available potassium was determined by atomic absorption method, the nitrate nitrogen was determined by flow analyzer, the soil pH value was measured by pH meter, the soil EC value was measured by EC meter (Ma Mingze), and the available zinc and iron contents in the soil were determined by atomic absorption method according to NY / T 890-2004.

[0107] Table 3 Effects on the soil nutrients of soybeans

[0108]

[0109] Compared with the comparative example, the examples can significantly increase the nutrient content in the soil, which is beneficial to the growth and development of soybeans; among them, the soil organic matter content of Example 2 is the highest, followed by Example 3 and Example 1, which are increased by 2.88, 0.87, 2.01 g / kg compared with Comparative Example 1.

[0110] In summary, compared with the prior art, the microbial amino acid water-soluble fertilizer prepared by the preparation method provided by the present invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility, promote the root development of crops, and improve the resistance of crops to adversity.

[0111] In addition, those skilled in the art should understand that although there are many problems in the prior art, each embodiment or technical solution of the present invention can be improved in only one or several aspects, and it is not necessary to solve all the technical problems listed in the prior art or the background art at the same time. Those skilled in the art should understand that the content not mentioned in a claim should not be regarded as a limitation to that claim.

[0112] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features. These modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a water-soluble fertilizer containing microbial amino acids, characterized in that: The following steps are involved: providing a first corn steep liquor and a second corn steep liquor; Adding a composite bacterial agent of Trichoderma harzianum and Pseudomonas lilacinus and lactic acid bacteria to the first corn steep liquor for first fermentation to obtain a first fermentation liquid; mixing the first fermentation liquid and the second corn steep liquor to obtain a mixed corn steep liquor; adding Bacillus subtilis to the mixed corn steep liquor for second fermentation to obtain a second fermentation liquid; Nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer are added to the second fermentation liquid in sequence to obtain a water-soluble fertilizer containing microbial amino acids.

2. The method for preparing a water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: The mass ratio of the first corn slurry to the second corn slurry is 1:

1.

3. The method for preparing a water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: The mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the composite bacterial agent of Trichoderma harzianum and Pseudomonas lilacinus is 1000:1; and / or The mass ratio of the sum of the first corn steep liquor, the second corn steep liquor and the lactic acid bacteria is 1800:

1.

4. The method for preparing the water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: The mass ratio of the sum of the first corn steep liquor, the second corn steep liquor and the Bacillus subtilis is 200:

1.

5. The method for preparing the water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: The temperature of the first fermentation is 20-30° C. and the time of the first fermentation is 7 days; and / or The temperature of the second fermentation is 25-30°C, and the time of the second fermentation is 7 days.

6. The method for preparing the water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: The mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the nitrogen fertilizer is (25:4)-(10:1); Preferably, the nitrogen fertilizer is selected from any one of nitrate nitrogen, ammonium nitrogen, and amide nitrogen, or a combination of at least two of them.

7. The method for preparing a water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: The mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the phosphate fertilizer is (20:1)-(50:1); Preferably, the phosphate fertilizer is selected from any one of monoammonium phosphate, diammonium phosphate, and potassium dihydrogen phosphate, or a combination of at least two of them.

8. The method for preparing the water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: The mass ratio of the sum of the first corn steep liquor and the second corn steep liquor to the potash fertilizer is 100:3; Preferably, the potash fertilizer is selected from any one of potassium chloride, potassium sulfate, potassium nitrate, or a combination of at least two of them.

9. The method for preparing a water-soluble fertilizer containing microbial amino acids according to claim 1, characterized in that: After the nitrogen fertilizer is added, the phosphorus fertilizer and the potassium fertilizer are added in sequence at least 2 hours apart.

10. A water-soluble fertilizer containing microbial amino acids obtained according to the method described in any one of claims 1 to 9.

Images