Feed containing monosodium glutamate byproduct and production method thereof

Abstract

The invention discloses a feed containing monosodium glutamate by-products and a production method of the feed, and belongs to the technical field of feeds. The fertilizer is prepared from the following raw materials in parts by weight: 60-100 parts of distillers' grains, 20-40 parts of corn sugar residues, 5-12 parts of monosodium glutamate wastewater, 3-8 parts of microbial zymophyte, 15-40 parts of water, 4-10 parts of a traditional Chinese medicine preparation and 8-16 parts of a carrier, wherein the carrier is modified zeolite composite particles. According to the prepared feed containing the monosodium glutamate by-product, recycling of resources is achieved, the storage stability of the feed is improved, the shelf life of the feed is prolonged, meanwhile, the immune antitoxic ability of animals can be improved, and animal growth can be promoted.

Description

Technical Field

[0001] The invention relates to the technical field of feed, and in particular to a feed containing a monosodium glutamate byproduct and a production method thereof. Background Art

[0002] MSG wastewater is an amino acid liquid produced when corn deep processing enterprises produce monosodium glutamate (MSG). Its crude protein (dry matter) is very high, but it contains a lot of non-protein nitrogen, which limits the application of this amino acid liquid in feed.

[0003] At present, the main method of utilizing monosodium glutamate wastewater is to mix it with other carriers and then dry it to produce protein raw materials with high non-protein nitrogen, such as glutamic acid residue, sprayed corn husk, sprayed corn gluten powder, etc. However, these methods have the defect of high non-protein content. Monogastric animals cannot effectively utilize non-protein nitrogen, and the amount added in feed formula is very limited.

[0004] Based on the principle that ruminants can digest some non-protein nitrogen, we use the byproduct of monosodium glutamate, monosodium glutamate wastewater, to produce a product that can be compared with corn dry distillers grains containing soluble matter, distillers grains protein feed (DDGS). The main raw materials of the product are corn sugar residue, monosodium glutamate wastewater, and other carriers. Wet corn sugar residue can provide crude fat and carbon source, and monosodium glutamate wastewater can provide crude protein and nitrogen source. After mixing with yeast, solid-state fermentation is carried out to achieve the production of high-fat protein raw materials with better color, aroma, taste, and indicators than DDGS.

[0005] Chinese invention patent number CN104256056A discloses an animal feed prepared from glutamic acid fermentation waste. Step 1) filtration of fermentation liquid, step 2) decolorization, concentration and crystallization, step 3) hydrolysis, step 4) preparation of composite powder, step 5) preparation of main ingredients, and step 6) preparation of feed. Although the feed prepared by the invention effectively utilizes amino acid fermentation waste, reduces feed production costs, and ensures the edible safety of meat products, the shelf life of the invention is very short and difficult to store for a long time. Summary of the invention

[0006] In view of the above-mentioned deficiencies in the prior art, the present invention provides a feed containing monosodium glutamate by-product and a production method thereof.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention is: A feed containing monosodium glutamate byproducts is composed of the following raw materials: distiller's grains, corn sugar residues, monosodium glutamate wastewater, microbial fermentation bacteria, water, traditional Chinese medicine preparations, and carriers.

[0008] Preferably, the feed containing monosodium glutamate by-product is composed of the following raw materials in parts by weight: 60-100 parts by weight of distiller's grains, 20-40 parts by weight of corn sugar residues, 5-12 parts by weight of monosodium glutamate wastewater, 3-8 parts by weight of microbial fermentation bacteria, 15-40 parts by weight of water, 4-10 parts by weight of traditional Chinese medicine preparations, and 8-16 parts by weight of carriers.

[0009] In the present invention, the monosodium glutamate wastewater refers to the organic wastewater generated in the monosodium glutamate production process, and the wastewater includes high-concentration monosodium glutamate wastewater, and the COD value of the high-concentration monosodium glutamate wastewater is 80000-100000 mg / L, and the NH3-N value is 28000-30000 mg / L.

[0010] Adding MSG wastewater as a nitrogen source to the vinegar lees culture medium, using the growth metabolism of microbial fermentation bacteria to convert inorganic nitrogen into organic nitrogen, thereby increasing the true protein content in the feed.

[0011] The microbial fermentation bacteria is at least one of Bacillus lysinicus HQ08, Bacillus coagulans, and Kluyveromyces marxianus; preferably, the microbial fermentation bacteria is composed of Bacillus lysinicus HQ08, Bacillus coagulans, and Kluyveromyces marxianus in a mass ratio of (2-4): (1-3): 1; further, the microbial fermentation bacteria is composed of Bacillus lysinicus HQ08, Bacillus coagulans, and Kluyveromyces marxianus in a mass ratio of 3:2:1.

[0012] The preparation method of the traditional Chinese medicine preparation comprises the following steps: respectively taking 10-30 parts by weight of astragalus, 10-20 parts by weight of calamus, 5-15 parts by weight of andrographis paniculata, 10-20 parts by weight of panax notoginseng, 10-20 parts by weight of angelica, 10-20 parts by weight of coptis chinensis and 10-20 parts by weight of liquorice, washing with water, drying, crushing and passing through a 60-100 mesh sieve to obtain traditional Chinese medicine powder; adding 300-500 parts by weight of water to the traditional Chinese medicine powder, soaking for 0.5-2h, placing in boiling water for heating for 2-5h and filtering to obtain filter residue and filtrate; adding 300-500 parts by weight of water to the filter residue, placing in boiling water for heating for 1-3h and filtering, combining the two filtrates, and concentrating the filtrate under reduced pressure to 1 / 4 of the original volume to obtain the traditional Chinese medicine preparation.

[0013] The Chinese medicine used in the present invention: Astragalus: slight smell, slightly sweet taste, invigorating qi and raising yang, consolidating the exterior and stopping sweating, promoting diuresis and reducing swelling, promoting the production of body fluid and nourishing blood, and relieving stagnation and relieving arthritis; Acorus calamus: aromatic smell, bitter and slightly pungent taste, invigorating the mind and resolving phlegm, refreshing the mind and improving intelligence, removing dampness and stimulating appetite; Andrographis paniculata: enters the heart, lung, large intestine, and bladder meridians, clears away heat and detoxifies, cools blood, and relieves swelling and relieves pain; Panax notoginseng: enters the liver and stomach meridians, disperses blood stasis and stops bleeding, relieves swelling and relieves pain, and is antibacterial; Angelica sinensis, sweet in taste, warm, enters the heart, liver, and spleen meridians, invigorates blood and harmonizes blood, regulates menstruation and relieves pain, moisturizes and lubricates the intestines; Coptis chinensis: enters the heart, spleen, stomach, liver, gallbladder, and large intestine meridians, has the effects of clearing heat and detoxifying, antibacterial and anti-inflammatory, has a broad-spectrum antibacterial effect, and has a significant inhibitory effect on a variety of cocci and bacilli; Licorice: enters the heart, lung, spleen, and stomach meridians, invigorates the spleen and replenishes qi, clears away heat and detoxifies, relieves urgency and relieves pain, and harmonizes various medicines.

[0014] The effects of the Chinese medicine preparation used in the present invention are as follows: the present invention uses Astragalus and Panax notoginseng to enhance the immunity of animals, and is considered to have the potential to improve the function of the animal immune system. They may help reduce the risk of animal diseases and improve growth performance; Coptis chinensis can be used for anti-inflammatory, which may help reduce the inflammatory response in animals and promote health; Angelica sinensis is traditionally believed to have the effect of nourishing blood, which may help improve the hemoglobin level of animals and improve blood quality; Licorice Chinese medicine is considered to have an anti-stress effect, which helps to alleviate physiological disorders caused by environmental pressure, diseases, etc. Simultaneous use can have a positive effect on the digestive system of animals, promote the absorption and utilization of nutrients, reduce dependence on antibiotics, and improve the natural resistance of animals to diseases. After the Chinese medicine preparation and microbial fermentation bacteria are combined for fermentation, on the one hand, they can play their respective positive roles, and on the other hand, the Chinese medicine components in the fermentation process can provide nutrients for bacterial growth, reduce the toxicity of Chinese medicine, and have a synergistic effect. The Chinese medicine preparation is composed of Astragalus, Acorus calamus, Andrographis paniculata, Panax notoginseng, Angelica sinensis, Coptis chinensis, and Licorice, which can improve disease resistance and immunity and promote the digestion and absorption of feed.

[0015] Feed is prone to mold and adsorb mycotoxins when left for a long time. Modified zeolite can effectively inhibit mold growth, significantly reduce mycotoxin content, extend feed storage period, and at the same time enhance animals' immune resistance and promote animal growth.

[0016] Zeolite is a hydrated aluminosilicate composed of silicon-oxygen tetrahedron and aluminum-oxygen tetrahedron. It has a uniform pore structure and abundant pores inside the crystal, which gives it a huge surface area and a certain adsorption capacity. However, due to the small adsorption capacity of the original zeolite, it needs to be frequently regenerated, which affects its adsorption performance and needs to be modified. The role of modified zeolite can also give full play to the active ingredients in traditional Chinese medicine that play a pharmacological role. The production process of this additive is simple, the overall cost is low, and it can be directly added to the diet for use, greatly improving the immunity of animals.

[0017] The present invention modifies zeolite, uses malic acid and organic iron ions as cationic composite modifiers, and utilizes iron ion intercalation modification to replace the original place-occupying ions of zeolite crystals. After the treatment, the pore size or crystal structure of the zeolite can be changed, the internal surface area can be increased, and more surface binding sites can be exposed, which can not only improve the adsorption capacity of mycotoxins in feed, but also improve the trace nutrients essential for animal growth and development.

[0018] The carrier is modified zeolite or modified zeolite composite particles.

[0019] The preparation method of the modified zeolite is as follows: S1. Wash 8-14 parts by weight of zeolite powder with water, dry it, add 20-30wt% hydrochloric acid to the dried zeolite powder according to the material-liquid ratio of 1g:(20-40)ml, stir at room temperature and 300-500rpm for 0.5-2h, filter and wash it to obtain pretreated zeolite powder; S2 adds the above-mentioned pretreated zeolite powder to a 0.5-2 mol / L aqueous solution of ferric citrate, wherein the mass ratio of the pretreated zeolite powder to ferric citrate is 1:1, stirs at 50-70°C and 300-500rpm for 1-3h, filters and dries, calcines at 450-600°C for 3-6h, and cools to obtain a first-level modified zeolite powder; adds 3-6 parts by weight of a 40-60mg / mL malic acid aqueous solution to the first-level modified zeolite powder, stirs at 50-70°C and 300-500rpm for 1-3h, filters, washes, dries and passes through a 100-300 mesh sieve to obtain a modified zeolite.

[0020] Preferably, the preparation method of the modified zeolite composite particles is as follows: S1: washing the zeolite powder with water, drying it, adding hydrochloric acid to the dried zeolite powder, stirring it, filtering it, washing it, and obtaining the pretreated zeolite powder; S2: adding the above-mentioned pretreated zeolite powder to the ferric citrate aqueous solution, stirring, filtering, drying, calcining, cooling, and obtaining a first-level modified zeolite powder; adding malic acid aqueous solution to the first-level modified zeolite powder, stirring, suction filtering, washing, drying, and sieving to obtain a modified zeolite; S3: adding the modified zeolite to the ethanol aqueous solution and stirring, then adding the essential oil particles, continuing to stir, centrifuging, and drying to obtain the zeolite composite particles.

[0021] The present invention first performs acid leaching treatment on zeolite powder, which is helpful to eliminate soluble impurities in the zeolite, which is helpful to improve the purity of the zeolite on the one hand, and on the other hand, it is helpful to expand the pore volume of the zeolite and improve the pore structure. The modified zeolite is obtained by iron doping and then modified by malic acid. The modified zeolite has a larger specific surface area and porosity, which is helpful to improve the adsorption of essential oil particles and better play the role of the essential oil particles.

[0022] The present invention makes plant essential oil into nanoparticles. In order to improve the stability of plant essential oil and improve the stability in water, the essential oil molecules can be evenly dispersed in the feed, and can be continuously and slowly released into the feed to play a mildew-proof role, thereby improving the storage stability of the feed. Compared with directly adding lipophilic and water-insoluble essential oil particles to the feed, the modified zeolite is used to adsorb the essential oil particles and then add them to the feed, which helps to improve the stability of grapefruit essential oil and garlic essential oil, reduce the volatility and decomposition rate, and improve the utilization rate of the essential oil particles, thereby promoting the development of the digestive system of animals and enabling animals to better absorb nutrients; the essential oil particles are allowed to enter the animal body for circulation, thereby further improving the animal's immune ability, reducing the diarrhea rate of animals to a certain extent, thereby promoting the growth of animals.

[0023] Furthermore, the preparation method of the modified zeolite composite particles is as follows: S1. Wash 8-14 parts by weight of zeolite powder with water, dry it, add 20-30wt% hydrochloric acid to the dried zeolite powder according to the material-liquid ratio of 1g:(20-40)ml, stir at room temperature and 300-500rpm for 0.5-2h, filter and wash it to obtain pretreated zeolite powder; S2: adding the above-mentioned pretreated zeolite powder to a 0.5-2 mol / L aqueous solution of ferric citrate, wherein the mass ratio of the pretreated zeolite powder to the ferric citrate is 1:1, stirring at 50-70°C and 300-500rpm for 1-3h, filtering, drying, calcining at 450-600°C for 3-6h, cooling, and obtaining a first-level modified zeolite powder; adding 3-6 parts by weight of a 40-60mg / mL malic acid aqueous solution to the first-level modified zeolite powder, stirring at 50-70°C and 300-500rpm for 1-3h, suction filtering, washing, drying, and passing through a 100-300 mesh sieve to obtain a modified zeolite; S3: add 100-200 parts by weight of 35-60wt% ethanol aqueous solution to the modified zeolite, stir at 300-500rpm for 20-60min, add 2-5 parts by weight of essential oil particles, continue stirring for 3-6h, centrifuge, and dry to obtain zeolite composite particles.

[0024] The preparation method of the essential oil particles is as follows: 1-3 parts by weight of grapefruit essential oil, 1-3 parts by weight of garlic essential oil, 0.5-2 parts by weight of cyclodextrin, and 0.5-2 parts by weight of choline chloride are mixed and stirred to obtain the particles.

[0025] The method for producing the feed containing the monosodium glutamate by-product comprises the following steps: (1) Weigh the above raw materials in parts by weight; (2) mixing corn sugar residue, monosodium glutamate wastewater and water, and stirring at 100-400 rpm for 40-60 min to obtain a mixture; (3) Dry the distiller's grains to a moisture content of 8-15%, crush them, pass them through a 80-20 mesh sieve to obtain distiller's grains particles, add them to the above mixture, stir them at 200-400 rpm for 20-50 min, sterilize them at 110-130° C. for 15-30 min, inoculate them with microbial fermentation bacteria, seal and ferment them at 30-37° C. for 8-14 d, add a carrier and stir them at 300-500 rpm for 50-80 min, and after conditioning, granulation, drying, cooling and packaging, a feed containing monosodium glutamate by-product is obtained.

[0026] Beneficial effects of the invention: The invention provides a feed containing monosodium glutamate byproducts and a production method, which reduces the cost of feed production and realizes the transformation of waste into treasure. The raw materials are distiller's grains, corn sugar residue, monosodium glutamate wastewater, microbial fermentation bacteria, Chinese medicine preparations, carriers, etc. as feed raw materials, and a variety of Chinese medicine ingredients are added to the Chinese medicine preparations. Among them, the glycosides, flavonoids, polysaccharides, lipid substances and other active ingredients contained in astragalus, calamus, andrographis paniculata, notoginseng, angelica, coptis root, and licorice have the effect of promoting antibody production and immune regulation, have good antiviral and antibacterial effects, can inhibit the growth of pathogenic bacteria, thereby greatly improving the immune ability of the animal body and promoting the digestion and absorption of nutrients. The addition of carriers can not only improve the stability of plant essential oils, allowing the essential oil molecules to be evenly dispersed in the feed, and can be continuously and slowly released into the feed to play an anti-mildew role and improve the storage stability of the feed; it can also improve the utilization rate of essential oil particles, thereby promoting the development of the animal's digestive system and enabling the animal to better absorb nutrients; it allows the essential oil particles to enter the animal's body circulation, thereby further improving the animal's immunity, reducing the animal's diarrhea rate to a certain extent, and promoting the animal's growth. DETAILED DESCRIPTION

[0027] The above content of the present invention is further described in detail below in conjunction with specific implementation modes, but this should not be understood as the scope of the above subject matter of the present invention being limited to the following embodiments.

[0028] Introduction of some raw materials in this application: The lees were purchased from Shandong Yiguo New Materials Co., Ltd., with a crude fat content of 12%, a crude fiber content of 8%, and a crude protein content of 28%.

[0029] Corn sugar residue was purchased from Huamo (Shandong) Biotechnology Co., Ltd.

[0030] Lysinibacillus HQ08 was provided by the General Microbiology Center of China Microorganism Culture Collection Administration, with the number CGMCC No.24221.

[0031] Bacillus coagulans was provided by China General Microorganism Culture Collection Center, with the number CGMCC No.1.6576.

[0032] Kluyveromyces marxianus was provided by the General Microbiology Center of China Culture Collection Administration of Microorganisms, with the number CGMCC No.24455.

[0033] The commercially available conventional feed in the control group was purchased from Shouguang Baijulong Biological Feed Co., Ltd., and the nutritional components were as follows: moisture content: 10%, crude protein: 24%, crude fiber: 8.0%, crude fat: 2.0%, and amino acid content: 22.1%.

[0034] Example 1

[0035] A feed containing monosodium glutamate byproduct is composed of the following raw materials in parts by weight: 80 parts by weight of distiller's grains, 25 parts by weight of corn sugar residues, 6 parts by weight of monosodium glutamate wastewater, 5 parts by weight of microbial fermentation bacteria, 20 parts by weight of water, 6 parts by weight of traditional Chinese medicine preparations, and 10 parts by weight of carriers.

[0036] The microbial fermentation bacteria are composed of Bacillus lysinicus HQ08, Bacillus coagulans and Kluyveromyces marxianus in a mass ratio of 3:2:1.

[0037] The preparation method of the traditional Chinese medicine preparation comprises the following steps: respectively taking 20 parts by weight of astragalus, 15 parts by weight of calamus, 10 parts by weight of andrographis paniculata, 10 parts by weight of panax notoginseng, 10 parts by weight of angelica, 15 parts by weight of coptis chinensis and 15 parts by weight of liquorice, washing with water, drying, crushing and passing through an 80-mesh sieve to obtain traditional Chinese medicine powder; adding 400 parts by weight of water to the traditional Chinese medicine powder, soaking for 1 hour, heating at 100°C for 3 hours and filtering to obtain filter residue and filtrate; adding 400 parts by weight of water to the filter residue, heating at 100°C for 2 hours and filtering, combining the two filtrates, and concentrating the filtrate under reduced pressure to 1 / 4 of the original volume to obtain the traditional Chinese medicine preparation.

[0038] The carrier is zeolite.

[0039] The method for producing the feed containing the monosodium glutamate by-product comprises the following steps: (1) Weigh the above raw materials in parts by weight; (2) mixing corn sugar residue, monosodium glutamate wastewater and water, and stirring at 200 rpm for 45 minutes to obtain a mixture; (3) Dry the distiller's grains to a moisture content of 10%, crush them, and pass them through a 100-mesh sieve to obtain distiller's grains particles, add them to the above mixture, stir them at 300 rpm for 30 min, sterilize them at 121°C for 20 min, inoculate them with microbial fermentation bacteria, seal and ferment them at 35°C for 10 d, add a carrier and stir them at 400 rpm for 60 min, and after conditioning, granulation, drying, cooling and packaging, you can get a feed containing monosodium glutamate by-products.

[0040] Example 2

[0041] A feed containing monosodium glutamate byproduct is composed of the following raw materials in parts by weight: 80 parts by weight of distiller's grains, 25 parts by weight of corn sugar residues, 6 parts by weight of monosodium glutamate wastewater, 5 parts by weight of microbial fermentation bacteria, 20 parts by weight of water, 6 parts by weight of traditional Chinese medicine preparations, and 10 parts by weight of carriers.

[0042] The microbial fermentation bacteria are composed of Bacillus lysinicus HQ08, Bacillus coagulans and Kluyveromyces marxianus in a mass ratio of 3:2:1.

[0043] The preparation method of the traditional Chinese medicine preparation comprises the following steps: respectively taking 20 parts by weight of astragalus, 15 parts by weight of calamus, 10 parts by weight of andrographis paniculata, 10 parts by weight of panax notoginseng, 10 parts by weight of angelica, 15 parts by weight of coptis chinensis and 15 parts by weight of liquorice, washing with water, drying, crushing and passing through an 80-mesh sieve to obtain traditional Chinese medicine powder; adding 400 parts by weight of water to the traditional Chinese medicine powder, soaking for 1 hour, heating at 100°C for 3 hours and filtering to obtain filter residue and filtrate; adding 400 parts by weight of water to the filter residue, heating at 100°C for 2 hours and filtering, combining the two filtrates, and concentrating the filtrate under reduced pressure to 1 / 4 of the original volume to obtain the traditional Chinese medicine preparation.

[0044] The carrier is a modified zeolite, and the preparation method of the modified zeolite is as follows: S1: 10 parts by weight of zeolite powder was washed with water and dried, and 25 wt% hydrochloric acid was added to the dried zeolite powder according to a material-liquid ratio of 1 g: 25 ml, and stirred at room temperature and 400 rpm for 1 hour, filtered, and washed to obtain pretreated zeolite powder; S2: Add the above-mentioned pretreated zeolite powder to a 1 mol / L aqueous solution of ferric citrate, wherein the mass ratio of the pretreated zeolite powder to ferric citrate is 1:1, stir at 60°C and 400 rpm for 2 h, filter and dry, calcine at 500°C for 4 h, and cool to obtain a first-level modified zeolite powder; add 5 parts by weight of a 50 mg / mL aqueous solution of malic acid to the first-level modified zeolite powder, stir at 60°C and 400 rpm for 2 h, filter, wash, and dry through a 200-mesh sieve to obtain a modified zeolite.

[0045] The method for producing the feed containing the monosodium glutamate by-product comprises the following steps: (1) Weigh the above raw materials in parts by weight; (2) mixing corn sugar residue, monosodium glutamate wastewater and water, and stirring at 200 rpm for 45 minutes to obtain a mixture; (3) Dry the distiller's grains to a moisture content of 10%, crush them, and pass them through a 100-mesh sieve to obtain distiller's grains particles, add them to the above mixture, stir them at 300 rpm for 30 min, sterilize them at 121°C for 20 min, inoculate them with microbial fermentation bacteria, seal and ferment them at 35°C for 10 d, add a carrier and stir them at 400 rpm for 60 min, and after conditioning, granulation, drying, cooling and packaging, you can get a feed containing monosodium glutamate by-products.

[0046] Example 3

[0047] A feed containing monosodium glutamate byproduct is composed of the following raw materials in parts by weight: 80 parts by weight of distiller's grains, 25 parts by weight of corn sugar residues, 6 parts by weight of monosodium glutamate wastewater, 5 parts by weight of microbial fermentation bacteria, 20 parts by weight of water, 6 parts by weight of traditional Chinese medicine preparations, and 10 parts by weight of carriers.

[0048] The microbial fermentation bacteria are composed of Bacillus lysinicus HQ08, Bacillus coagulans and Kluyveromyces marxianus in a mass ratio of 3:2:1.

[0049] The preparation method of the traditional Chinese medicine preparation comprises the following steps: respectively taking 20 parts by weight of astragalus, 15 parts by weight of calamus, 10 parts by weight of andrographis paniculata, 10 parts by weight of panax notoginseng, 10 parts by weight of angelica, 15 parts by weight of coptis chinensis and 15 parts by weight of liquorice, washing with water, drying, crushing and passing through an 80-mesh sieve to obtain traditional Chinese medicine powder; adding 400 parts by weight of water to the traditional Chinese medicine powder, soaking for 1 hour, heating at 100°C for 3 hours and filtering to obtain filter residue and filtrate; adding 400 parts by weight of water to the filter residue, heating at 100°C for 2 hours and filtering, combining the two filtrates, and concentrating the filtrate under reduced pressure to 1 / 4 of the original volume to obtain the traditional Chinese medicine preparation.

[0050] The carrier is a modified zeolite composite particle, and the preparation method of the modified zeolite composite particle is as follows: S1: 10 parts by weight of zeolite powder was washed with water and dried, and 25 wt% hydrochloric acid was added to the dried zeolite powder according to a material-liquid ratio of 1 g: 25 ml, and stirred at room temperature and 400 rpm for 1 hour, filtered, and washed to obtain pretreated zeolite powder; S2: adding the above-mentioned pretreated zeolite powder to a 1 mol / L aqueous solution of ferric citrate, wherein the mass ratio of the pretreated zeolite powder to the ferric citrate is 1:1, stirring at 60°C and 400 rpm for 2 h, filtering, drying, calcining at 500°C for 4 h, cooling, and obtaining a first-level modified zeolite powder; adding 5 parts by weight of a 50 mg / mL aqueous solution of malic acid to the first-level modified zeolite powder, stirring at 60°C and 400 rpm for 2 h, suction filtering, washing, drying, and passing through a 200-mesh sieve to obtain a modified zeolite; S3: Take the modified zeolite and add 120 parts by weight of 40 wt% ethanol aqueous solution, stir at 400 rpm for 30 min, then add 3 parts by weight of essential oil particles, continue stirring for 4 h, centrifuge, and dry to obtain zeolite composite particles.

[0051] The preparation method of the essential oil particles is as follows: 2 parts by weight of grapefruit essential oil, 2 parts by weight of garlic essential oil, 1 part by weight of cyclodextrin and 1 part by weight of choline chloride are mixed and stirred to obtain the particles.

[0052] The method for producing the feed containing the monosodium glutamate by-product comprises the following steps: (1) Weigh the above raw materials in parts by weight; (2) mixing corn sugar residue, monosodium glutamate wastewater and water, and stirring at 200 rpm for 45 minutes to obtain a mixture; (3) Dry the distiller's grains to a moisture content of 10%, crush them, and pass them through a 100-mesh sieve to obtain distiller's grains particles, add them to the above mixture, stir them at 300 rpm for 30 min, sterilize them at 121°C for 20 min, inoculate them with microbial fermentation bacteria, seal and ferment them at 35°C for 10 d, add a carrier and stir them at 400 rpm for 60 min, and after conditioning, granulation, drying, cooling and packaging, you can get a feed containing monosodium glutamate by-products.

[0053] Example 4

[0054] A feed containing monosodium glutamate byproduct is composed of the following raw materials in parts by weight: 80 parts by weight of distiller's grains, 25 parts by weight of corn sugar residues, 6 parts by weight of monosodium glutamate wastewater, 5 parts by weight of microbial fermentation bacteria, 20 parts by weight of water, 6 parts by weight of traditional Chinese medicine preparations, and 10 parts by weight of carriers.

[0055] The microbial fermentation bacteria are composed of Bacillus lysinicus HQ08, Bacillus coagulans and Kluyveromyces marxianus in a mass ratio of 3:2:1.

[0056] The preparation method of the traditional Chinese medicine preparation comprises the following steps: respectively taking 20 parts by weight of astragalus, 15 parts by weight of calamus, 10 parts by weight of andrographis paniculata, 10 parts by weight of panax notoginseng, 10 parts by weight of angelica, 15 parts by weight of coptis chinensis and 15 parts by weight of liquorice, washing with water, drying, crushing and passing through an 80-mesh sieve to obtain traditional Chinese medicine powder; adding 400 parts by weight of water to the traditional Chinese medicine powder, soaking for 1 hour, heating at 100°C for 3 hours and filtering to obtain filter residue and filtrate; adding 400 parts by weight of water to the filter residue, heating at 100°C for 2 hours and filtering, combining the two filtrates, and concentrating the filtrate under reduced pressure to 1 / 4 of the original volume to obtain the traditional Chinese medicine preparation.

[0057] The carrier is a modified zeolite composite particle, and the preparation method of the modified zeolite composite particle is as follows: S1: 10 parts by weight of zeolite powder was washed with water and dried, and 25 wt% hydrochloric acid was added to the dried zeolite powder according to a material-liquid ratio of 1 g: 25 ml, and stirred at room temperature and 400 rpm for 1 hour, filtered, and washed to obtain pretreated zeolite powder; S2: Take the above-mentioned pretreated zeolite powder and add 120 parts by weight of 40wt% ethanol aqueous solution, stir at 400rpm for 30min, then add 3 parts by weight of essential oil particles, continue stirring for 4h, centrifuge, and dry to obtain zeolite composite particles.

[0058] The preparation method of the essential oil particles is as follows: 2 parts by weight of grapefruit essential oil, 2 parts by weight of garlic essential oil, 1 part by weight of cyclodextrin and 1 part by weight of choline chloride are mixed and stirred to obtain the particles.

[0059] The method for producing the feed containing the monosodium glutamate by-product comprises the following steps: (1) Weigh the above raw materials in parts by weight; (2) mixing corn sugar residue, monosodium glutamate wastewater and water, and stirring at 200 rpm for 45 minutes to obtain a mixture; (3) Dry the distiller's grains to a moisture content of 10%, crush them, and pass them through a 100-mesh sieve to obtain distiller's grains particles, add them to the above mixture, stir them at 300 rpm for 30 min, sterilize them at 121°C for 20 min, inoculate them with microbial fermentation bacteria, seal and ferment them at 35°C for 10 d, add a carrier and stir them at 400 rpm for 60 min, and after conditioning, granulation, drying, cooling and packaging, you can get a feed containing monosodium glutamate by-products.

[0060] Example 5

[0061] A feed containing monosodium glutamate byproduct is composed of the following raw materials in parts by weight: 80 parts by weight of distiller's grains, 25 parts by weight of corn sugar residues, 6 parts by weight of monosodium glutamate waste water, 5 parts by weight of microbial fermentation bacteria, 26 parts by weight of water, and 10 parts by weight of a carrier.

[0062] The microbial fermentation bacteria are composed of Bacillus lysinicus HQ08, Bacillus coagulans and Kluyveromyces marxianus in a mass ratio of 3:2:1.

[0063] The carrier is zeolite.

[0064] The method for producing the feed containing the monosodium glutamate by-product comprises the following steps: (1) Weigh the above raw materials in parts by weight; (2) mixing corn sugar residue, monosodium glutamate wastewater and water, and stirring at 200 rpm for 45 minutes to obtain a mixture; (3) Dry the distiller's grains to a moisture content of 10%, crush them, and pass them through a 100-mesh sieve to obtain distiller's grains particles, add them to the above mixture, stir them at 300 rpm for 30 min, sterilize them at 121°C for 20 min, inoculate them with microbial fermentation bacteria, seal and ferment them at 35°C for 10 d, add a carrier and stir them at 400 rpm for 60 min, and after conditioning, granulation, drying, cooling and packaging, you can get a feed containing monosodium glutamate by-products.

[0065] Test Example 1

[0066] Growth performance test method: 120 28-day-old weaned piglets (half male and half female) in good health were selected and randomly divided into 6 groups (5 test groups and 1 control group). The control group was fed with commercially available pig feed, and the remaining 5 test groups were fed with feed containing monosodium glutamate byproducts prepared in Examples 1-5. The test period was 30 days. All test growing pigs were free to eat and drink water. The feeding method followed the principle of small amounts and multiple times. Feed was added every day according to the schedule of 6:00, 11:00, 16:00, and 21:00. Attention was paid to ventilation and temperature to ensure that the average house temperature was 25°C. Growth indicators were recorded: daily weight gain, daily feed intake, number of diarrhea heads, and meat-to-feed ratio. The results are shown in Table 1.

[0067] Table 1. Growth performance Daily weight gain (kg / d) Daily feed intake (kg / d) Diarrhea frequency Meat to feed ratio (F / G) Example 1 0.31 0.56 5 2.59 Example 2 0.37 0.63 3 2.17 Example 3 0.41 0.76 0 1.89 Example 4 0.38 0.71 1 2.03 Example 5 0.28 0.51 10 2.96 Control group 0.27 0.49 15 2.99

[0068] Test Example 2 Detection method: Take 60 g of the feed containing monosodium glutamate by-products prepared in Examples 1-4 of the present invention, adjust the water content to 25%, add it to a culture dish and spread it flat, add 20 ml of a 0.01% aqueous solution of Aspergillus niger and Aspergillus green spores, respectively, culture it at 35°C and 70% humidity for 36 hours, store it at 40°C±0.5°C in the dark for 30 days, and then test its stability after storage for 1 day and 30 days.

[0069] Among them: "-" means that no hyphae growth can be seen on the surface of the feed and there is no mold; "+" means that there is slight hyphae on the surface of the feed, but no hyphae are seen inside, which is mild mold; "++" means that the mold on the surface of the feed is heavier, but no hyphae are seen inside, which is moderate mold; "+++" means that the mold on the surface is serious and hyphae are seen inside, which is severe mold. The test results are shown in Table 2.

[0070] Table 2. Anti-mildew performance test results

[0071] It can be seen from the above results that the feed containing monosodium glutamate byproducts prepared by the present invention can promote the growth of pigs compared with conventional feeds available on the market. Comparing Example 1 and Example 4, it can be seen that the Chinese medicine preparation used in the present invention can provide nutrients for bacterial growth, has a synergistic effect, can have a positive impact on the digestive system of animals, promote the absorption and utilization of nutrients, improve the natural resistance of animals to diseases, and can effectively promote the growth of pigs. Feed is prone to mold and adsorb mycotoxins when left for a long time, while modified zeolite can effectively inhibit mold growth, significantly reduce the content of mycotoxins, prolong the storage period of feed, and at the same time can enhance the immune resistance of animals and promote animal growth.

[0072] As can be seen from Examples 1-2, the zeolite is modified by using malic acid and organic iron ions as cationic composite modifiers. After modification by iron ion intercalation, the original place-occupying ions of the zeolite crystal are replaced. After treatment, the pore size or crystal structure of the zeolite can be changed, the internal surface area can be increased, and more surface binding sites can be exposed, which can not only improve the adsorption capacity of mycotoxins in feed; at the same time, it increases the trace nutrients that are essential for animal growth and development. The modified zeolite can also give full play to the active ingredients in traditional Chinese medicine that play a pharmacological role.

[0073] As can be seen from Examples 2-4, the present invention makes plant essential oils into nanoparticles, which not only improves the stability of plant essential oils, but also improves the stability in water. Essential oil molecules can be evenly dispersed in the feed, and can be continuously and slowly released into the feed to play an anti-mildew effect, thereby improving the storage stability of the feed. Compared with directly adding lipophilic, water-insoluble essential oil particles to the feed, the use of modified zeolite to adsorb essential oil particles and then add them to the feed helps to improve the stability of grapefruit essential oil and garlic essential oil, reduce the volatile decomposition rate, and improve the utilization rate of essential oil particles, thereby promoting the development of the animal's digestive system and enabling the animal to better absorb nutrients; thereby further improving the animal's immune ability, reducing the animal's diarrhea rate to a certain extent, and promoting the growth of the animal.

Claims

1. A feed containing monosodium glutamate by-product, characterized in that: The invention is composed of the following raw materials: distiller's grains, corn sugar residue, monosodium glutamate wastewater, microbial fermentation bacteria, water, traditional Chinese medicine preparation, and carrier; the carrier is modified zeolite composite particles; the preparation method of the modified zeolite composite particles is as follows: S1: washing the zeolite powder with water, drying it, adding hydrochloric acid to the dried zeolite powder, stirring it, filtering it, washing it, and obtaining the pretreated zeolite powder; S2: adding the above-mentioned pretreated zeolite powder to the ferric citrate aqueous solution, stirring, filtering, drying, calcining, cooling, and obtaining a first-level modified zeolite powder; adding malic acid aqueous solution to the first-level modified zeolite powder, stirring, suction filtering, washing, drying, and sieving to obtain a modified zeolite; S3: adding the modified zeolite to the ethanol aqueous solution and stirring, then adding the essential oil particles, continuing to stir, centrifuging, and drying to obtain the zeolite composite particles.

2. The feed containing monosodium glutamate by-product according to claim 1, characterized in that: The method comprises the following raw materials in parts by weight: 60-100 parts by weight of distiller's grains, 20-40 parts by weight of corn sugar residue, 5-12 parts by weight of monosodium glutamate wastewater, 3-8 parts by weight of microbial fermentation bacteria, 15-40 parts by weight of water, 4-10 parts by weight of traditional Chinese medicine preparations, and 8-16 parts by weight of carriers; The carrier is a modified zeolite composite particle; the preparation method of the modified zeolite composite particle is as follows: S1: washing the zeolite powder with water, drying it, adding hydrochloric acid to the dried zeolite powder, stirring it, filtering it, washing it, and obtaining the pretreated zeolite powder; S2: adding the above-mentioned pretreated zeolite powder to the ferric citrate aqueous solution, stirring, filtering, drying, calcining, cooling, and obtaining a first-level modified zeolite powder; adding malic acid aqueous solution to the first-level modified zeolite powder, stirring, suction filtering, washing, drying, and sieving to obtain a modified zeolite; S3: adding the modified zeolite to the ethanol aqueous solution and stirring, then adding the essential oil particles, continuing to stir, centrifuging, and drying to obtain the zeolite composite particles.

3. The feed containing monosodium glutamate by-product according to claim 1, characterized in that: The microbial fermentation bacteria is at least one of Bacillus lysinicus HQ08, Bacillus coagulans, and Kluyveromyces marxianus.

4. The feed containing monosodium glutamate byproduct according to claim 1, characterized in that: The preparation method of the traditional Chinese medicine preparation comprises the following steps: washing astragalus, calamus, andrographis paniculata, notoginseng, angelica, coptis root and liquorice with water, drying and crushing to obtain the traditional Chinese medicine powder; adding water to soak the traditional Chinese medicine powder, heating and filtering to obtain filter residue and filtrate; adding water to the filter residue, heating, filtering, combining the filtrate, and concentrating under reduced pressure to obtain the traditional Chinese medicine preparation.

5. The feed containing monosodium glutamate by-product according to claim 1, characterized in that: The preparation method of the essential oil particles is as follows: grapefruit essential oil, garlic essential oil, cyclodextrin and choline chloride are mixed and stirred to obtain the essential oil particles.

6. The method for producing feed containing monosodium glutamate byproduct according to any one of claims 1 to 5, characterized in that: The steps include: (1) Weigh the above raw materials in parts by weight; (2) mixing corn sugar residue, monosodium glutamate wastewater and water to obtain a mixture; (3) Drying, crushing and sieving the distiller's grains to obtain distiller's grains particles, adding the particles to the above mixture, stirring, sterilizing, inoculating microbial fermentation bacteria and sealing for fermentation, adding the carrier and stirring, and subjecting the mixture to conditioning, granulating, drying, cooling and packaging to obtain a feed containing monosodium glutamate by-product.

Images