Rapid cultivation device and method of daphnia magna laboratory

Abstract

The invention provides a rapid cultivation device and method of a daphnia magna laboratory and belongs to the field of cultivation of zooplankter. The rapid cultivation device comprises supports, base plates, water bath tanks, a water storage tank, cultivation cups, heating rods, thermometers, turbulence pumps, a fluorescent lamp and a time controller. Each base plate is arranged at the bottom of the corresponding support. The storage water tank is arranged on the base plate of the bottommost support. The water bath tanks are arranged on the base plates of the two upper supports. One thermometer, one turbulence pump and two heating rods are arranged in the water storage tank. Each water bath tank is provided with one thermometer, two heating rods and six cultivation cups. The fluorescent lamp is located above the portion deviating from the center of the support and is parallel to the support. The time controller is in contact with the fluorescent lamp. The rapid cultivation device is easy and convenient to operate, high in operability, easy to set up and free of a complicated device structure. Daphnia magna cultivated through the rapid cultivation device is rapid in reproduction and large in quantity, the life condition of the daphnia magna is stable, and the individual difference of the daphnia magna is small, so that results of toxic tests of fleas are stable and reliable, the test quality is guaranteed, and enough test materials can be provided for tests such as removal of zooplankter pollution to water in the industry of water treatment at the same time.

Description

technical field [0001] The invention belongs to the field of zooplankton culture, and relates to a culture device and a culture method of Daphnia magna. Background technique [0002] Daphnia magna belongs to Arthropoda, Crustacea, and Cladocera. It is a common freshwater zooplankton and plays an important role in aquatic ecosystems. Because of its wide distribution, short life cycle, fast reproduction speed, high reproductive capacity, easy cultivation, and sensitivity to poisons, it is widely used in aquatic toxicity tests. [0003] At present, Daphnia magna is an internationally recognized standard test indicator organism. At the same time, many countries have established standard methods for testing Daphnia magna toxicity, and have made relevant regulations on the cultivation conditions of Daphnia magna. However, at present, the cultivation of Daphnia magna in most laboratories in my country lacks effective culture conditions or devices, and it is difficult to ensure the...

AI Technical Summary

Problems solved by technology

However, at present, the cultivation of Daphnia magna in most laboratories in my country lacks effective culture conditions or devices, and it is difficult to ensure the cultivation effect. This makes the cultivation environment of Daphnia magna not stable enough, it is difficult to maintain parthenogenesis for a long time, and the number of Daphnia magna is difficult to guarantee

Therefore, it is difficult to obtain the magna fleas required for the test, such as different ages of the magna fleas, individuals not neat enough, and different life states, etc., which will have a great impact on the test results, and the quality of the test cannot be guaranteed

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