The invention provides a preparation method and application of a
potamogeton crispus alpha-
glucosidase inhibitor for reducing
blood sugar. Fresh
potamogeton crispus stone buds and / or fresh
potamogeton crispus are / is washed, dried, smashed and sieved to obtain
raw material powder, 80% ethyl
alcohol is used as a decolorizing agent to be mixed with the
raw material powder, a precipitate 1 is obtained through ultrasonic-assisted decolorization, heat preservation and
centrifugation, the precipitate 1 continues to be treated with the 80% ethyl
alcohol in the same mode, and a precipitate 2 is obtained; adding water into the precipitate 2 for swelling, adjusting the pH value, carrying out ultrasonic-assisted extraction, heating extraction and
centrifugation, repeating the operation on the precipitate twice, merging the three supernatants, adjusting the pH value, and carrying out vacuum concentration to obtain a concentrated solution; adding
ethanol into the concentrated solution for
alcohol precipitation, centrifuging to obtain a precipitate 6, adding water into the precipitate 6 for dissolving, adjusting the pH value, standing and centrifuging to obtain a precipitate 7, namely the potamogeton crispus extract. The potamogeton crispus extract can significantly inhibit the activity of alpha-
glucosidase, can be used as an alpha-
glucosidase inhibitor, and is used for preparing drugs for improving
insulin resistance, drugs for treating
diabetes mellitus and drugs for reducing
blood sugar.