Curly pondweed rigid bud tissue culturing method

Abstract

The invention relates to the technology field of plant tissue culture, wherein potamogeton crispus turions tissue culture and rapid multiplication technology technique is employed for the mass production of high grade potamogeton crispus tissue culture sprouts, which has overcome the problem of different seed germination under natural conditions, thus meeting the need of recovering aquatic vegetation, developing herbivorous fishes, purifying water and introducing seeds into lakes and ponds.

Description

1. Technical field

[0001] The invention belongs to the technical field of plant tissue culture. 2. Background technology

[0002] The pollution of my country's water ecosystem is serious, and the decline of water environment quality has caused serious damage to aquatic biological resources. It is of great significance to restore the balance of aquatic ecosystem, purify water quality, promote the development of fishery, and introduce water purification plants.

[0003] Potamogeton crispus L. is a perennial submerged herbaceous plant of Potamogetonaceae, which has high nutritional value and economic value. Smilax has strong adaptability to water eutrophication, and can also grow densely in water bodies seriously polluted by domestic sewage. It has the function of purifying water bodies and is the main aquatic plant for purifying water quality from winter to early summer. However, because the seeds are extremely difficult to germinate under natural conditions, the growth of st...

Examples

Embodiment Construction

[0019] 1. Establish a sterile system

[0020] After washing the pedicel buds with tap water, soak them in 70% ethanol for 30s, and wash them with 0.1% HgCl 2 Aqueous solution containing a small amount of Tween 80, sterilized for 9 minutes, and washed 5 times with sterile distilled water. The sterilized stone buds were inoculated on the MS medium without exogenous hormones added with 3% sucrose, and an aseptic system was established under the conditions of temperature 25±2°C, light intensity 2000lx, and daily light 12h.

[0021] 2. Method for Tissue Culture of Limegrass Lithium Buds

[0022] The frequency of adventitious bud induction can be increased to 100% through the regulation of hormones.

[0023] The establishment of a sterile system is the key to determine whether it can successfully induce differentiation. By adjusting and improving the explant sterilization treatment conditions and the state of the culture medium, the browning and pollution of the explants have bee...

1. Technical field

[0001] The invention belongs to the technical field of plant tissue culture. 2. Background technology

[0002] The pollution of my country's water ecosystem is serious, and the decline of water environment quality has caused serious damage to aquatic biological resources. It is of great significance to restore the balance of aquatic ecosystem, purify water quality, promote the development of fishery, and introduce water purification plants.

[0003] Potamogeton crispus L. is a perennial submerged herbaceous plant of Potamogetonaceae, which has high nutritional value and economic value. Smilax has strong adaptability to water eutrophication, and can also grow densely in water bodies seriously polluted by domestic sewage. It has the function of purifying water bodies and is the main aquatic plant for purifying water quality from winter to early summer. However, because the seeds are extremely difficult to germinate under natural conditions, the growth of st...

Examples

Embodiment Construction

[0019] 1. Establish a sterile system

[0020] After washing the pedicel buds with tap water, soak them in 70% ethanol for 30s, and wash them with 0.1% HgCl 2 Aqueous solution containing a small amount of Tween 80, sterilized for 9 minutes, and washed 5 times with sterile distilled water. The sterilized stone buds were inoculated on the MS medium without exogenous hormones added with 3% sucrose, and an aseptic system was established under the conditions of temperature 25±2°C, light intensity 2000lx, and daily light 12h.

[0021] 2. Method for Tissue Culture of Limegrass Lithium Buds

[0022] The frequency of adventitious bud induction can be increased to 100% through the regulation of hormones.

[0023] The establishment of a sterile system is the key to determine whether it can successfully induce differentiation. By adjusting and improving the explant sterilization treatment conditions and the state of the culture medium, the browning and pollution of the explants have bee...