The application belongs to the technical field of
plant tissue culture and particularly relates to a rapid propagation method of Guoguang aseptic seedlings of apple. The propagation method is realized through the following steps: after the stem segments after
cutting and cleaning are sterilized, the stems are subjected to
bud peeling, post-sterilization treatment and
cutting, and then
germination, the germinated buds are subjected to proliferation and rooting culture. The Guoguang aseptic
seedling system and the
tissue culture and rapid propagation
system thereof are established for the first time, which lays an important foundation for scientific research and agricultural production development of Guoguang apple, promotes the
sustainable development of Guoguang apple, and provides important support for
apple seed industry safety. The rapid propagation method can improve the
germination rate, reduce the
browning rate in the culture process, shorten the time required for propagation, realize the rapid propagation of Guoguang apple in a short time, realize the rapid improvement of Guoguang, accelerate the breeding speed of new varieties, and improve the breeding efficiency and success rate.