The application belongs to the technical field of
plant genetic engineering, and particularly relates to a genetic transformation method of 'Hanfu'
apple seed callus and application. The transformed
callus is obtained through induction culture, proliferation culture, immersion culture, co-culture and screening culture of the explant, wherein the explant is 'Hanfu'
apple seed; the uninfected 'Hanfu'
apple seed callus induced by the method has weak
fluorescence signal under
ultraviolet excitation light. When the callus is subjected to genetic transformation mediated by
Agrobacterium and the pRI101 GFP vector is introduced, the
fluorescence signal intensity of the callus is significantly increased, indicating that the 'Hanfu' apple callus can be subjected to genetic transformation containing a GFP
label.