The present invention discloses a preparation method of nymphaea hybrid Pu'er tea. The nymphaea hybrid Pu'er tea is prepared from the raw or cooked Pu'er tea and dried nymphaea hybrid as raw materials, and by the steps of flower selecting, flower petal selecting, tea leaf selecting, kneading and blending, flower shaping, bag cake kneading, pressing and shaping, cooling and drying, and packaging and storing. The weight ratio of the Pu'er tea to the dried nymphaea hybrid is (2-4):(0.5-1.5). The method comprises the following steps: the Pu'er tea and the dried nymphaea hybrid are respectively weighted, the weighed materials are mixed, the mixture is rolled, the rolled mixture is fumigated, then the fumigated mixture is put into bags to conduct the cake kneading, the kneaded mixture is loaded into molds, the shaped nymphaea hybrid is added into the molded mixture, the mixture is pressed and shaped for some time, the pressed and shaped mixture is prepared into the nymphaea hybrid Pu'er tea with different weights, a qualified inspection report is provided, and the nymphaea hybrid Pu'er tea is packaged. The nymphaea hybrid Pu'er tea combines the special effects of the nymphaea hybrid. In the preparation process, the traditional preparation technology of the Pu'er tea is used, and the effects of the flowers and tea are fully retained, so that the effects, soup color and mouthfeel of the nymphaea hybrid and Pu'er tea are perfectly combined, the functional ingredients of the nymphaea hybrid are fully released, the production technology is simple, and the nymphaea hybrid Pu'er tea can be subjected to a large-scale production.