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Hybridization method for nymphaea

A hybrid method and water lily technology, applied in the field of artificial hybrid breeding of water lilies, can solve the problems of low seed setting rate, complicated operation, easy injury and stigma, and achieve the effect of improving the success rate

Active Publication Date: 2019-05-17
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of complex operation, low efficiency, easy damage to the stigma and low seed setting rate of the existing technology, the present invention creates a pollination liquid for pollination, which can greatly improve the cross-subgenus cross-pollination efficiency and fruiting rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for cross-subgenus cross-breeding of water lilies with high efficiency and high seed setting rate is carried out in the following steps:

[0032] (1) According to the subgenus and color of the required water lily, the breeding target is confirmed, and the wide-tropical water lily named "Blue Goddess" is selected as the male parent, and the cold-resistant water lily named "Fenzhuang" is used as the female parent Book;

[0033] (2) male parent bagging: when the sepals loosened one day before the male parent bloomed, the male parent flower buds were bagged with a transparent bag;

[0034] (3) female parent bagging: the day before the female parent is about to open (the outer petals start to change color, and the sepals are slightly opened) to carry out bagging with a transparent bag;

[0035] (4) Preparation of pollination solution: prepare 50 mL of pure water, 2.5 g of sucrose, 0.05 g of naphthaleneacetic acid, 75% alcohol, put sucrose and NAA dissolved in alcoh...

Embodiment 2

[0043] A method for cross-subgenus cross-breeding of water lilies with high efficiency and high success rate is carried out in the following steps:

[0044] (1) According to the subgenus and color of the required water lily, the breeding target is confirmed, and the subtropical water lily named "Josephine" is selected as the male parent in the Longtan Shuiyi scenic spot in Nanjing, and the cold resistance is named "Wanweisha" water lily as female parent;

[0045] (2) male parent bagging: the male parent flower bud is carried out bagging process with transparent bag before the sepal loosens one day before the male parent blooms;

[0046] (3) Female parent bagging: carry out bagging process with transparent bag one day before female parent is about to open;

[0047] (4) Preparation of pollination solution: prepare 50 mL of pure water, 2.5 g of sucrose, 0.05 g of naphthaleneacetic acid, 75% alcohol, put sucrose and NAA dissolved in alcohol into pure water and stir evenly, prepar...

Embodiment 3

[0055] A kind of embodiment of the method for cross-subgenus cross-breeding of water lilies with high efficiency and high success rate is carried out as follows:

[0056] (1) According to the subgenus and color of the required water lily, the breeding target is confirmed, and the tropical water lily named "Tina" is selected as the male parent in the Longtan Shuiyi scenic spot in Nanjing, and the ancient tropical water lily named "Thai powder" is used as the male parent. female parent;

[0057] (2) male parent bagging: carry out bagging process to male parent flower bud with transparent bag before male parent sepal loosens;

[0058] (3) Female parent bagging: carry out bagging process with transparent bag one day before female parent is about to open;

[0059] (4) Preparation of pollination solution: prepare 50 mL of pure water, 2.5 g of sucrose, 0.05 g of naphthaleneacetic acid, 75% alcohol, put sucrose and NAA dissolved in alcohol into pure water and stir evenly, prepare pol...

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Abstract

The invention discloses a hybridization method for nymphaea. The method comprises the following steps: (1), matching hybridization male and female parents, determining a breeding objective, and then selecting male and female parents which can perform fructification and meet characters of the breeding objective; (2), bagging flower buds of the male parent; (3), bagging the female parent one day before the female parent is about to come into bloom; (4), preparing 30 to 50 ml of pure water, 2.0 to 3.0 g of saccharose, 0.05 g of naphthylacetic acid and ethyl alcohol into pollination liquid; (5), collecting pollen, uniformly stirring the pollination liquid, firstly soaking a cotton swab into the pollination liquid, and then dipping the cotton swab into a stamen of the mature male parent; (6), performing pollination, dipping the cotton swab which dips pollen to a stigma of the female parent, and slight brushing the stigma for a couple of times; then covering a pollination bag, and covering agauze bag to an outmost layer; marking information, and marking with white foam. The pollination method is low in technical requirement on pollination, but is high in efficiency and success rate.

Description

technical field [0001] The invention belongs to the technical field of water lily artificial hybridization and breeding, and relates to a high-efficiency water lily hybridization method. Background technique [0002] Water lily is a perennial aquatic herbaceous plant of the genus Nymphaea in the family Nymphaeaceae. It is known as the "Sleeping Beauty Among Flowers". It has high ornamental value, economic value and unique cultural connotation, and is deeply loved by people. There are more than 50 native species in the world. According to the ecological type classification, they are generally divided into two categories: tropical water lily and cold-resistant water lily; according to subgenus classification, they are generally divided into five subgenuses: wide-tropical, ancient tropical, cold-resistant, Australian, and neotropical. Mainly distributed in tropical, subtropical and temperate regions, 5 species are native to my country. After long-term selection and breeding by ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 徐迎春陈磊王珍陆薇芳金奇江王彦杰
Owner NANJING AGRICULTURAL UNIVERSITY
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