28results about How to "Improve seed setting rate" patented technology

The invention discloses application of OsHTT4 protein in regulation and control of heat resistance and yield of rice. According to the invention, a CRISPR / Cas9 gene editing system is utilized to perform mutation on a rice OsHTT4 gene, and an OsHTT4 protein function deletion mutant strain is constructed. The heat stress of the obtained mutant strain shows that the tolerance of the rice to the heat stress can be remarkably improved by inhibiting the expression of the OsHTT4 protein. In addition, by inhibiting the expression of the OsHTT4 protein, the grain number per ear, the yield per plant, the tiller number and the maturing rate of the rice can be remarkably improved, and the rice yield is improved. On the basis, the invention further provides a method for improving the heat stress tolerance and / or yield of the rice, and the method is beneficial to cultivation of heat stress-resistant and high-yield rice varieties.

The invention provides a method for rapidly breeding a nonglutinous rice restorer line with japonica rice consanguinity. According to the method, a high-quality high-yield japonica rice three-line sterile line is used as a female parent, a plurality of high-quality disease-resistant indica rice varieties are used as male parents, and a large number of different combinations of F1 seeds are obtained through pairwise hybridization. The combination with high maturing rate is screened from the F2 generation, is planted and selected generation by generation, and is backcrossed with the indica rice variety for multiple times, and finally, the indica rice restorer line with japonica rice consanguinity is quickly obtained. According to the method, parent sources are expanded, genetic diversity is improved, manual castration operation is omitted, hybridization efficiency is improved, and the breeding period is remarkably shortened. The bred restorer line has the characteristics of high yield, disease resistance, high maturing rate and excellent agronomic traits, and is suitable for large-scale agricultural production and application.

The invention discloses application of soybean GmABCG5L gene in improving plant seed setting rate, and belongs to the technical field of plant breeding. In order to solve the problem of how to promote plants to improve seed setting rate, the invention provides a method for promoting plants to improve seed setting rate, wherein a recombinant vector is obtained by constructing a GmABCG5L gene and connecting it with a pROK vector, the recombinant vector is transferred into Agrobacterium to prepare Agrobacterium carrying the GmABCG5L gene, and then a transgenic plant carrying the GmABCG5L gene is obtained by using an Agrobacterium-mediated leaf disc method, and by culturing the transgenic plant carrying the GmABCG5L gene, it is found that the transgenic plant carrying the GmABCG5L gene has a good growth state, an enlarged inflorescence compared with a wild type, an increased number of florets, and a significantly improved total seed amount compared with a wild type, indicating that the GmABCG5L gene can improve the plant seed setting rate.

The invention provides a plant growth regulator for improving the maturing rate of leymus chinensis and a spraying method of the plant growth regulator. The plant growth regulator for improving the maturing rate of Chinese wildrye comprises one or more of 2, 4-dichlorphenoxyacetic acid, indole-3-acetic acid, gibberellin and 6-benayl aminopurine. The concentration of the 2, 4-dichlorphenoxyacetic acid is 10 mg / L, the concentration of the indole-3-acetic acid is 2.8 mg / L, the concentration of the gibberellin is 2.5 mg / L, and the concentration of the 6-benayl aminopurine is 5 mg / L. The plant growth regulator for improving the maturing rate of leymus chinensis and the spraying method thereof have the advantages of being high in preparation efficiency and relatively simple in spraying method.

The invention discloses a soapberry SmSEP3 gene and application thereof, the gene is expressed in a plant to regulate and control the flowering time of the plant and promote pistil development, sepal curling and pod development, and the gene is a nucleotide sequence as shown in a sequence table Seq ID NO.1 or a nucleotide sequence of an amino acid sequence as shown in a coding sequence table Seq ID NO.2. The gene is used for cultivating new soapberry varieties with different flowering times, different flower types and larger fruits. Through the analysis of transgenic arabidopsis thaliana, the bolting and flowering time of the plant can be advanced through the overexpression of the arabidopsis thaliana, the pistil is obviously lengthened, sepals are curled, and the fruit volume is larger. The flowering time, bolting days and rosette leaf number of the transgenic arabidopsis thaliana plant are obviously different from those of a wild plant, pistil development is longer, fruit pods are increased, the breeding cycle of the sapindus tree species is accelerated, ornamental varieties of different flower types are cultivated, and cultivation of high-yield new varieties is accelerated.

This invention discloses a plant growth regulator composition for improving rice quality and resistance to stress, and its preparation method. It relates to the fields of plant growth regulators and agricultural stress-resistant cultivation technology. The plant growth regulator composition is a water-dispersible granule or wettable powder, comprising, by weight percentage: 0.05%-0.30% 5-aminolevulinic acid, 1.0%-5.0% γ-aminobutyric acid, 8.0%-20.0% betaine, 3.0%-10.0% trehalose, 2.0%-8.0% chitosan oligosaccharide, 1.0%-5.0% fucoidan oligosaccharide, and salicylic acid or its agricultural grade. Acceptable components include 0.05%–0.50% salt, 20.0%–45.0% potassium dihydrogen phosphate, 3.0%–15.0% water-dispersible silica, 0.5%–3.0% amino acid chelated zinc, 3.0%–10.0% magnesium sulfate, 0.5%–3.0% wetting and dispersing agent, 1.0%–6.0% binder, with the balance being water-soluble filler. Porous water-dispersible silica is used as a carrier to allow sensitive active substances such as 5-aminolevulinate and salicylic acid to enter the carrier pores and be immobilized in the protective matrix formed by chitosan oligosaccharide and trehalose.

The invention relates to the technical field of application of agricultural pollination insects, in particular to a field pollination method for megachile megachile. The invention provides a field pollination method for megachile megachile. The method comprises the following steps: a preparation stage: preparing incubation, arranging a honeycomb and preparing a nest plate; in the pollination stage, megachile clover is released, nesting conditions are observed, and disease and pest control is carried out; and a recovery treatment stage. The method can effectively improve the fruiting performance of alfalfa.

The invention provides a buckwheat stress resistance and lodging resistance yield-increasing regulator and a preparation method and application thereof, belonging to the technical field of plant growth regulators. The buckwheat stress resistance and lodging resistance yield-increasing regulator uses water as a solvent and comprises the following components in concentrations: 7.5-10.5 g / L sodium p-chlorophenoxyacetate, 125-245 g / L cyprodinil, 70-200 g / L polyaspartate and 18-22 ml / L spreading agent. The buckwheat stress resistance and lodging resistance yield-increasing regulator can induce buckwheat to establish a huge root system, improve root system activity, increase the toughness of buckwheat stalks, reduce plant height, and improve buckwheat's ability to resist lodging; shorten branch length, improve canopy structure and function, improve buckwheat leaf photosynthetic performance and spike development; improve buckwheat stress resistance, improve pollen and stigma activity, extend the effective pollination time, and improve pollination rate and fruit setting rate; and promote buckwheat to obtain high and stable yields.

The present invention provides a method for breeding all-female varieties of industrial hemp, which comprises the following steps: after land preparation of an isolated plot, seeds of male-inducing plants are sown in early April, and male-inducing plants are grown after two transplantings; seeds of field plants are sown in early to mid-May, and field plants are grown after two transplantings, and artificial male induction is performed in early to mid-June, and after three field pollinations, field plant grains are harvested in early October. The present invention adopts means such as staged sowing, seedling raising in seedling nutrient blocks, two transplantings, male-inducing plants and batch management of field plants, chemical male induction, artificial pollination, etc., to effectively solve the technical problem of breeding all-female varieties of industrial hemp.

The invention discloses a fertilizer preparation containing phytosterol ester and a use method of the fertilizer preparation. The fertilizer preparation containing the phytosterol ester is prepared from the phytosterol ester and auxiliary materials, the content of the phytosterol ester in the fertilizer preparation containing the phytosterol ester is 0.001 percent to 99.999 percent; according to the fertilizer preparation disclosed by the invention, by adding the phytosterol ester and utilizing the unique molecular structure of the phytosterol ester, the activity of related physiological and biochemical reaction enzymes in cells is activated to resist environmental stress, promote photosynthesis of plant leaves, accelerate elongation and division of plant cells and promote root development; furthermore, absorption of plants to various nutrient substances is promoted, dry matter accumulation and sugar accumulation are promoted, flowering is promoted, the fruiting quantity is increased, and the yield is increased.

The present application relates to the technical field of green prevention and control of rice diseases and pests, and specifically discloses a compound traditional Chinese medicine preparation for preventing and treating apple snails, and a preparation method and application thereof. The compound traditional Chinese medicine preparation comprises the following components in parts by weight: radix veratri 15-25 parts, monkshood 10-20 parts, Chinese toona fruit 15-20 parts, capsicum 18-28 parts, kopsia 8-15 parts, mint 5-10 parts, and rhizoma haemorrhoidalis 3-8 parts. The present application uses natural traditional Chinese medicine materials, has no chemical pesticide components, and can be completely degraded naturally without destroying the ecological balance of the rice field. The present application has the advantages of fast and complete killing speed, long egg inhibition duration, high overall killing rate of apple snails, wide source and low price of traditional Chinese medicine materials, simple preparation process, convenient field operation, multiple target points, no drug resistance of apple snails, continuous use for years, stable and non-declining prevention and control effect, improvement of the water and soil environment of the rice field, inhibition of the occurrence of diseases and pests in the field, promotion of the growth of rice roots, and improvement of the seedling emergence rate and seed setting rate of rice.

The invention discloses a primer for detecting a specific InDel marker of rice OsSTP14 gene and its application, belonging to the technical field of plant molecular biology. The specific InDel marker primer of the invention comprises a forward primer OsSTP14-iF: CGCAATTATTTGACACGG and a reverse primer OsSTP14-iR: CGAAGTAGAGGGAGGAGGT. The specific InDel marker primer can be used to accurately screen rice germplasm with a high fruiting rate through ordinary PCR, which is of great significance for promoting high-yield rice breeding and accelerating the breeding process.

The invention discloses a molecular marker related to the seed setting rate of broccoli seeds and its application. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. The 51st position of the sequence has a C / T polymorphism. The SNP is located at the 37728588th position of the C6 chromosome of the broccoli HDEM (verson 1.0) reference genome. When the base sequence mutates from C to T, the corresponding broccoli seed setting rate is improved. The SNP molecular marker is used to screen broccoli germplasm with a high seed setting rate. The KASP primer is used for PCR amplification. The primer excites HEX fluorescence in broccoli materials with a low seed setting rate, and the SNP excites FAM fluorescence in broccoli materials with a high seed setting rate, thereby realizing early and rapid identification of broccoli germplasm resources by molecular biological means.

The invention discloses an automatic water-saving precise irrigation system for a paddy field, and particularly relates to the technical field of water-saving irrigation, which comprises an automatic water-saving irrigation area division module, an automatic water-saving irrigation monitoring equipment setting module, an automatic water-saving irrigation data acquisition module, an automatic water-saving irrigation analysis decision module and an automatic water-saving irrigation control module, and an automatic water-saving irrigation man-machine interaction module. The automatic water-saving irrigation area division module is used for determining a paddy field in a jurisdiction area as a target area, and dividing the target area into a plurality of monitoring sub-areas which are sequentially marked as 1, 2, 3,..., n; according to the method, the paddy field in the target area is monitored, the water demand of each stage is analyzed according to different growth and development stages, it is ensured that the paddy obtains the needed water in the growth process, and waste of the water is avoided. The growth environment of the rice is optimized, and growth obstacles caused by too much or too little water are reduced.

The present application belongs to the field of fertilizer preparation, and particularly relates to an active silicon-calcium-potassium-magnesium fertilizer, and preparation and application thereof. The active silicon-calcium-potassium-magnesium fertilizer contains biomass ash 60%, mineral humic acid 30% and binder 10% by weight. The active silicon-calcium-potassium-magnesium fertilizer is used as base fertilizer or topdressing. When used as base fertilizer, the application amount is 200 kg, and when used as topdressing, the application amount is 50 kg. The present application uses biomass ash of a biomass thermal power plant as the main raw material, adds appropriate amount of mineral humic acid, uses clay as binder, and uses granulation process to prepare granules, and then uses film spraying technology to inoculate functional bacterial agent. On the one hand, the present application solves the resource recycling of a large amount of biomass ash of a biomass power plant, and on the other hand, the present application improves the activity of silicon, calcium, potassium and magnesium in the product, and meets the needs of crops for silicon, calcium, potassium and magnesium.

The invention discloses a method for culturing and transplanting corn transgenic tissue culture seedlings. The method for culturing and transplanting the corn transgenic tissue culture seedlings comprises the steps of immature embryo inoculation, II-type embryonic callus induction, adventitious bud induction, multiplication culture, rooting culture, culture in soil, seedling hardening and transplanting. By adopting the culture and transplanting method provided by the invention, the Zun-dan No.1 corn is used as the explant, and the multiplication culture is carried out under the sterile condition, so that the multiplication is fast, the induction rate is high, the breeding time can be greatly shortened, a large number of tissue culture seedlings can be obtained in a short time, the breeding time is saved, the transgenic breeding process is accelerated, and the method is easy to implement, convenient to operate and suitable for industrial production. A genetic transformation system is easy to establish, technical reference can be provided for exogenous gene transformation and transgenic breeding application of Zun-single No.1 corn, new corn germplasm with beneficial genes can be formed, corn transgenic breeding requirements can be met, the maturing rate of tissue culture seedlings and the seed number of a single plant can be increased, and accordingly planting benefits are improved.

The invention relates to the technical field of durian planting, and particularly provides a durian fruit retention method. According to the invention, the two effective components brassinolide and compound sodium nitrophenolate are combined and compounded into the durian fruit retention liquid medicine, the action mechanisms in the durian fruit retention aspect are different, cross resistance does not exist, and after being combined, the two effective components are compounded into the liquid medicine, so that an obvious synergistic interaction effect is achieved; and the durian fruit retention effect is obviously superior to that of a single agent of brassinolide and compound sodium nitrophenolate. The liquid medicine is used for treating flowers in the flowering period, regulating and controlling the physiological mechanism of flower organs and cooperating with artificial supplementary pollination, so that the pollination and fertilization capacity of the flowers is improved, the flowering and fruit setting rate is obviously increased, physiological fruit falling is reduced, effective fruit retention is achieved, the commodity rate and the yield of fruits are increased, and economic benefits are increased.

The invention discloses a rice nitrogen efficient utilization gene OsNAC15, a protein coded by the gene OsNAC15 and application of the gene OsNAC15, and belongs to the technical field of molecular breeding. The invention discloses the effect of the OsNAC15 gene in efficient utilization of rice nitrogen for the first time, the overexpression of the rice OsNAC15 gene significantly improves the nitrogen utilization rate and yield of a plant, and the mutation of the OsNAC15 gene significantly reduces the nitrogen utilization rate and yield of the plant; the mutant and / or overexpressed OsNAC15 gene has the advantages that the plant development is normal, the regulation and control of the nitrogen nutrition utilization rate of the rice can be realized by precisely regulating and controlling the expression level of the gene, the biomass and yield of crops are further improved, the environmental pollution is reduced, and the application prospect in the agricultural field is wide. Especially in the aspect of cultivating crop varieties capable of efficiently absorbing and utilizing nitrogen fertilizer, such as rice, corn, wheat, soybean, cotton, rape and other key crops, the wide application of the strain is expected to lead the agricultural production to be more environment-friendly and efficient, and a powerful scientific and technological support is provided for solving global grain safety problems and environmental protection challenges.

The invention discloses a water-soluble fertilizer for inhibiting absorption and accumulation of all-metal cadmium of rice grain weight and a preparation method of the water-soluble fertilizer. The water-soluble fertilizer is prepared from the following components in parts by weight: 20 to 25 parts of biochemical fulvic acid, 35 to 40 parts of compound amino acid, 10 to 15 parts of zinc sulfate monohydrate, 3 to 6 parts of ferric sodium ethylene diamine tetraacetate, 0.6 to 0.9 part of manganese sodium ethylene diamine tetraacetate, 0.3 to 0.6 part of boric acid, 0.4 to 0.8 part of pH (Potential of Hydrogen) regulator, 0.7 to 1.2 parts of chelating agent, 0.8 to 1.2 parts of surfactant and 80 to 100 parts of water. The preparation method of the water-soluble fertilizer comprises the following steps: S1, adding biochemical fulvic acid and compound amino acid into water, stirring and dissolving to obtain mother liquor A, S2, adding a pH regulator into the mother liquor A, stirring and dissolving to obtain mother liquor B, s3, respectively adding a chelating agent, zinc sulfate monohydrate, ferric sodium ethylene diamine tetraacetate, manganese sodium ethylene diamine tetraacetate and boric acid into the mother liquor B, stirring and dissolving to obtain mother liquor C; and S4, adding a surfactant into the mother liquor C, stirring and dissolving to obtain the water-soluble fertilizer.

The present invention provides the application of AeMYB30 and / or AeUF3GaT1 in improving the fruit setting rate of golden sunflower, belonging to the field of plant cultivation and genetic engineering. In the present invention, the hyperoside improves the plant fruit setting rate through the mediation of the complex MYB30-UF3GaT1, and the plant fruit setting rate can be simply, quickly and efficiently improved by spraying the hyperoside preparation on the plant. Spraying the hyperoside preparation of the present invention can significantly improve the fruit setting rate and thousand-grain weight of understory economic plants. Taking golden sunflower as an example, the fruit setting rate can reach up to 87.4% and the thousand-grain weight can reach up to 80.3g.

The present application belongs to the field of agricultural biotechnology, and discloses the use of overexpression of rice SnRK1 alpha gene in improving plant resistance to abiotic stress and yield. The present application also discloses the use of rice SnRK1 alpha protein or its coding nucleic acid in improving plant resistance to abiotic stress and / or improving plant yield, comprising: constructing a recombinant plant expression vector containing the coding nucleic acid of rice SnRK1 alpha protein; transforming the constructed recombinant plant expression vector into plant tissue or plant cells; cultivating and screening the transgenic plants with improved resistance to abiotic stress or improved yield. The present application has very important theoretical and practical significance for improving and enhancing the stress resistance of rice, cultivating high-yield and stress-tolerant varieties, and accelerating the process of stress-resistant molecular breeding.

The invention discloses a method for producing rice haploids and application of the method in breeding, by editing one or more of OsFEB1, OsFEB2 and OsFEB3 genes in rice, the functions of the OsFEB1, OsFEB2 and / or OsFEB3 genes are lost, a rice haploid induction line is obtained, generation of haploids is induced through sexual reproduction, the gene sequence of the OsFEB1 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, and the gene sequence of the OsFEB3 is as shown in SEQ ID NO.2. And the gene sequence of the OsFEB3 is as shown in SEQ ID NO. 3. The haploid induction line obtained by the method provided by the invention has the capability of inducing generation of haploid plants for breeding. An RHG1 haploid induction line is combined with an MIME system, and an apomixis material is artificially created to fix the heterosis of rice.

The invention discloses a protein for controlling the grain number per ear of rice and a coding gene thereof, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: carrying out chemical mutagenesis on an indica rice restorer line 'Lehui 188' to obtain a mutant rub1, and carrying out high-throughput sequencing and MutMap analysis to obtain a candidate gene LOCOs04g41030 (OsBIR3). Researches show that the OsBIR3 gene significantly affects the number of branches on the upper portion of a rice panicle, and further affects the number of grains per panicle and the yield of rice. It is found for the first time that the OsBIR3 gene has the functions of positively regulating and controlling the number of branches on the upper portion of a rice spike, the number of grains per spike, the plant height, the maturing rate, the grain length and the thousand grain weight. The method lays a theoretical foundation for plant type improvement and genetic engineering breeding of rice, and has very important theoretical value and practical significance.

The invention provides a filler-free synergistic fertilizer and a preparation method thereof. The fertilizer comprises the following components in parts by weight: 240-300 parts of urea, 80-110 parts of potassium chloride, 55-80 parts of ammonium chloride, 4-7 parts of polyglutamic acid, 3-10 parts of polyaspartic acid, 2-5 parts of potassium fulvate, 3-6 parts of alginic acid, 2-5 parts of zinc sulfate and 2-6 parts of boric acid. According to the preparation process disclosed by the invention, the synergist can be uniformly and stably arranged in the internal structure of the ultrahigh-concentration fertilizer granules on the premise of not introducing any filler, and the ultrahigh-concentration fertilizer granules can be used for improving agronomic characters of crops, especially rice.

The invention belongs to the technical field of rice gene engineering, and particularly relates to a polyploidy rice high-seed setting gene OsTHS-A1 and application thereof in increasing the seed setting rate of polyploidy rice. The OsTHS-A1 is highly expressed in a rice meiosis period, a nucleotide sequence of a CDS region of the gene is shown as SEQ ID NO.1 in a sequence table, and an amino acid sequence of a protein coded by the gene is shown as SEQ ID NO.2 in the sequence table. Functional verification shows that expression of the OsTHS-A1 gene in the high-seed-setting polyploidy rice is inhibited, and the seed setting rate is remarkably reduced; when the OsTHS-A1 gene is excessively expressed in the low-fructification polyploidy rice, the maturing rate is remarkably increased. Through over-expression of the OsTHS-A1 gene, the setting rate of the low-setting polyploidy rice is effectively improved.

The utility model discloses a pollen spreading device, which belongs to the technical field of pollen spreading and comprises a second fixing plate, a first fixing plate fixedly connected to one side of the second fixing plate, a fixing groove fixedly connected to one side of the first fixing plate, a sliding plate slidably connected into the fixing groove, an empty groove formed in the sliding plate, and a storage box fixedly connected to the bottom of the sliding plate. One side of the storage box is fixedly connected with a connecting plate, one side of the first fixing plate is fixedly connected with an air cylinder, an output shaft of the air cylinder is fixedly connected with the connecting plate, and one side of the first fixing plate is fixedly connected with a third fixing plate. When the pollination device is used, the storage box is driven to move forwards, the movable plate is separated from the storage box, the pollen material falls into the spreading box, the amount of pollen spread every time can be controlled more accurately, the best pollination effect can be achieved, and the maturing rate and fruit quality of plants are improved.

The invention belongs to the technical field of interplanting of cumin and corn, and discloses a planting method for interplanting of cumin and corn through drip irrigation under mulch in southern Xinjiang, comprising the following steps: S1, preparation before sowing: applying a base fertilizer to soil, and performing closed weeding treatment; s2, selecting varieties and treating seeds; s3, mulching film laying and sowing; s4, management after sowing: first water is dripped after the cumin seeds are white, and fertilization is carried out along with water; controlling water and fertilizer before budding from whole seedlings of cumin; after cumin buds appear, second water is dripped, and fertilizer is applied along with water; sowing corn after the second water, dripping third water, and applying fertilizer along with the water; controlling water and fertilizer after third watering and before filling and expanding of the cumin seeds; dripping water after the cumin grains are filled and expanded; s5, cumin harvesting, wherein a harvester is used for threshing and storing; s6, corn single growth period management, wherein after cumin is harvested, water drips, and fertilizer is applied along with water; s7, harvesting the corn; the problems that in an existing cumin planting technology, benefits are low, and the resource utilization rate is low are solved.

The invention belongs to the technical field of plant genetic engineering and plant genetic improvement, and particularly relates to application of negative regulation of OsABA8ox1 in improvement of alkali resistance of rice and / or transfer level of rice stem sheath assimilates. The OsABA8ox1 gene is a newly found key gene for promoting NSC transport of the stem sheaths of the rice and is also a main gene for improving alkali resistance of the rice, the NSC transport efficiency of the stem sheaths can be improved through genetic improvement, the maturing rate can be increased, the harvest index can be increased, meanwhile, the stress resistance of the rice is enhanced, the alkali resistance of the rice is remarkably improved, and the application prospect is wide.