28 results about "Phytosterol esters" patented technology

The invention discloses a nanometer fiber yarn-based phytosterol Pickering emulsion as well as a preparation method and application thereof, and the nanometer fiber yarn-based phytosterol Pickering emulsion comprises the following components in parts by weight: 0.5-1 part of nanometer fiber yarn; 1 to 10 parts of phytosterol / phytosterol ester; 95 to 105 parts of a water phase component; 95 to 105 parts of an oil phase component; the nanofibers are extracted from agricultural wastes; according to the invention, the nanofiber is used as the only or main stabilizer of the phytosterol Pickering emulsion, so that the problems of solubility, emulsion stability and bioaccessibility of phytosterol can be solved at the same time; and efficient interface stability and steric hindrance stability are realized by utilizing a unique nano network structure of the nano fiber yarns, and a natural, safe and efficient phytosterol delivery system is provided.

The invention discloses diglyceride composite edible oil assisting in reducing blood sugar and blood fat and a preparation method thereof, and relates to the technical field of functional edible oil. The diglyceride composite edible oil for assisting in reducing blood sugar and blood fat is prepared from the following raw materials in parts by weight: 40 to 60 parts of high-purity diglyceride oil, 2 to 3 parts of modified flaxseed oil micro powder, 2 to 3 parts of modified camellia oleosa seed oil microcapsule powder, 5 to 8 parts of perilla seed oil, 2 to 5 parts of phytosterol ester, 1 to 3 parts of resistant dextrin, 0.1 to 0.5 part of rosemary extract and 0.05 to 0.15 part of potassium citrate. The compound edible oil is scientific in formula, and the high-purity diglyceride oil is used as a core and matched with various functional components such as the modified flaxseed oil micro powder to synergistically play a role in assisting in reducing blood sugar and blood fat. Through an exclusive modification process, gradient high-pressure homogenization and other standardized preparation processes, the stability and dispersion uniformity of the components are improved, the absorption rate of the active components is high, the storage life is long, no artificially synthesized additive is added, the food is safe to eat, and practicability and health are both considered.

The invention discloses flammulina velutipes polysaccharide nano-liposome as well as a preparation method and application thereof, and belongs to the technical field of biological functional materials. The preparation method specifically comprises the following steps: (1) sequentially performing hot water extraction, deproteinization, alcohol precipitation, dialysis and freeze-drying on flammulina velutipes raw material powder; (2) dissolving in a phosphoric acid buffer solution; (3) mixing and dissolving phospholipid, cationic lipid and a membrane regulator in an organic solvent; (4) mixing and ultrasonic treatment; and (5) carrying out reduced-pressure rotary evaporation, hydration with a phosphate buffer solution, ultrasonic treatment and extrusion. According to the flammulina velutipes polysaccharide nano-liposome and the preparation method thereof, cholesterol is replaced by phytosterol ester to prepare the flammulina velutipes polysaccharide nano-liposome, flammulina velutipes polysaccharide (FVP) is encapsulated in a water core in the liposome, the safety is higher, and the encapsulation efficiency, the storage stability and the immunocompetence of a system are improved. In-vitro experiments prove that the phytosterol ester lipidosome has the effect of promoting macrophage proliferation and nitric oxide generation, and the effect is obviously better than that of the traditional cholesterol lipidosome under the same condition.

The invention discloses a red yeast rice powder-phytosterol ester blood fat reducing formula and an embedding technology, and relates to the technical field of health food, the red yeast rice powder-phytosterol ester blood fat reducing formula comprises, by weight, 30-50 parts of red yeast rice powder, 20-40 parts of phytosterol ester, 10-15 parts of fructo-oligosaccharide, and 1-3 parts of a rosemary extract; through the scientifically verified compounding proportion, the competition of the two components in the intestinal absorption stage is avoided, and the bioavailability is improved. Fructo-oligosaccharide is used as prebiotics, so that intestinal health is promoted, and the absorption capacity of intestinal tracts to active ingredients is improved; the rosemary extract has an anti-oxidation effect, can prolong the oxidation induction period of phytosterol ester to 120 hours, can protect monacolin K and phytosterol ester in the red yeast rice powder from being damaged by external factors such as light and heat, and further improves the stability.

The application discloses a phytosterol and phytosterol ester complex agent and application thereof, wherein the complex agent comprises phytosterol and phytosterol ester with a mass ratio of 1:10. The application aims to improve the water solubility and bioavailability of phytosterol, realize synergistic effect by compounding phytosterol and phytosterol ester, interfere with the lipid acquisition of coccidia from multiple targets, up-regulate the expression of cecal tight junction protein to repair the intestinal barrier, inhibit the release of serum and cecal pro-inflammatory factors to relieve inflammatory reaction, regulate the expression of genes related to lipid synthesis and fatty acid oxidation to remodel lipid metabolism homeostasis, and then effectively inhibit the growth and development of coccidia, and significantly improve the growth performance of broilers infected with coccidia.

This invention discloses a camel milk formula powder for assisting in the regulation of blood lipids and its preparation process, belonging to the field of functional dairy product deep processing technology. This formula powder constructs a five-in-one synergistic lipid-regulating network of "exogenous absorption inhibition, endogenous synthesis regulation, oxidative stress protection, intestinal microecological regulation, and vascular endothelial protection." Its core components include a specific ratio of compounded phytosterol esters, microencapsulated complex oils, and medicinal and edible extracts. The preparation process employs stepwise low-temperature microencapsulation and cold spray agglomeration granulation technology to achieve full-process low-temperature protection of active ingredients, with lactoferrin retention ≥90% and ω-3 fatty acid bioavailability increased by more than 25%. This invention's product has comprehensive lipid-regulating effects, 3 times improved oxidative stability, and produces no oil residue or off-odor when mixed, making it suitable for long-term consumption by people with borderline elevated blood lipids. It can also achieve continuous industrial production with a batch mixing uniformity RSD ≤2%.

The invention relates to the technical field of food processing, in particular to a functional oligosaccharide-containing glycolipid double-control composite tablet candy, which is prepared from the following raw materials: functional oligosaccharide, a lipid control component, a sweet base, microcrystalline cellulose, magnesium stearate, monoglyceride and beta cyclodextrin, the functional oligosaccharide is selected from one or more of fructo-oligosaccharide, isomaltooligosaccharide and galactooligosaccharide; the fat control component is selected from one or more of phytosterol ester and conjugated linoleic acid glyceride; the sweet base material comprises maltitol and isomaltulose; the raw materials can also comprise a mulberry leaf extract. According to the invention, a compound system of the functional oligosaccharide, the special fat control component and the auxiliary sugar control component is adopted, and all the components form a synergistic effect, so that not only can the glycemic index be effectively reduced, but also the fat absorption can be obviously inhibited, the double control of the sugar and the fat in a real sense is realized, and compared with the existing single-function product, the efficacy is more comprehensive and more obvious.

The invention discloses a medical sterilizing, antagonistic-free and fat-retaining disinfectant hand sanitizer and a preparation method thereof, and relates to the technical field of hand sanitizers. CHG is used as a main bactericide, sophorolipid, CAB and alpha-glucan oligosaccharide are used as a composite surfactant, and the antagonistic effect of sterilization effect reduction caused by CHG wrapping after a conventional surfactant forms micelles is eliminated; meanwhile, phytosterol ester, glycosylglycerol and madecassoside are introduced as skin fat-retaining components, so that the sebum retention rate is greater than or equal to 95%, meanwhile, the antibacterial rate on staphylococcus aureus is still kept 100%, and the hand washing liquid is suitable for high-frequency hand washing of medical staff.

The invention discloses a multi-structure carrier of recombinant III-type humanized collagen microspheres as well as a preparation method and application of the multi-structure carrier. The multi-structure carrier comprises an inner phase, a middle phase and an outer phase, the recombinant III-type humanized collagen microspheres and hydrogenated lecithin form a chimera which is located in the inner phase; the middle phase is composed of three kinds of grease similar to skin structures, namely phytosterol ester, squalane and polyglycerol-3 diisostearate; the external phase is a sodium hyaluronate aqueous solution. Through W / O / W double emulsification design, recombinant III-type humanized collagen microspheres and hydrogenated lecithin form a chimera and then are wrapped heavily, so that the environmental stability, biocompatibility and transdermal performance of the recombinant III-type humanized collagen microspheres are improved, and the recombinant III-type humanized collagen microspheres have immediate moisturizing and slow-release effects and can be applied to preparation of a skin care product. The effects of long-acting slow release, efficient transdermal and three-dimensional repair of the recombinant III-type humanized collagen microspheres are achieved, and the recombinant III-type humanized collagen microspheres are suitable for high-end cosmetics or medical instruments.

The invention discloses hyaluronic acid modified astaxanthin liposome, a preparation method and application thereof and an oral medicine for treating CD44 overexpression diseases, and belongs to the technical field of biological materials. According to the technical scheme, hyaluronic acid modified astaxanthin lipidosome is combined with a CD44 receptor through hyaluronic acid, and astaxanthin is delivered to liver cancer cells over-expressed by the CD44 receptor in a targeted mode; according to the hyaluronic acid modified astaxanthin lipidosome, the hyaluronic acid modified lipidosome is used as a carrier of astaxanthin, and the hyaluronic acid modified astaxanthin lipidosome is delivered in an oral administration mode; the liposome comprises lecithin and phytosterol ester; and the hyaluronic acid modified astaxanthin liposome takes lecithin and phytosterol ester as wall materials. The invention is applied to the aspect of CD44 overexpression disease treatment, and solves the problem that the application of astaxanthin in preparation of oral medicines for treating CD44 overexpression diseases is limited due to the fact that astaxanthin cannot be well combined with a targeted biomarker CD44 receptor on liver cancer cells when astaxanthin is orally delivered in the prior art.

The invention discloses a wood nanocellulose hydrophobic oil-resistant agent as well as a preparation method and application thereof, and belongs to the technical field of oil-resistant agent preparation. According to the preparation method of the wood nanocellulose hydrophobic oil-resistant agent provided by the invention, a hydrogen bond acceptor, a Bronsted acid donor and a Lewis acid metal salt are adopted to construct a ternary eutectic solvent, a lignin network in chemi-mechanical pulp is cooperatively cracked, and a catalytic environment is provided; synchronously adding chemi-mechanical pulp and a bio-based modifier coumaric acid-phytosterol ester into the solvent, and integrally completing in-situ nanocrystallization dissociation and surface hydrophobic esterification functionalization of the fibers in an inert atmosphere; and carrying out shearing dispersion assisted by dihydrolevoglucosenone, centrifugal purification and reduced pressure distillation on the reaction mixture to obtain the wood nanocellulose hydrophobic oil-resistant agent. According to the method, synchronous construction of efficient nano dissociation and a modified catalytic environment of the chemi-mechanical pulp is achieved through the ternary eutectic solvent, a nano-crystallization-hydrophobic oil-resistant modification integrated process is achieved, toxic and harmful substances are avoided, and the method is green and environmentally friendly.

The invention relates to the technical field of microbial feed additives, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the steps that lactobacillus plantarum and / or enterococcus faecalis are / is used as strains for activation and expanding culture, agricultural and sideline product waste is used as a substrate for liquid-state deep fermentation, and mature fermentation liquor is obtained; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide, granulating, and pre-drying at low temperature to obtain a semi-dry particle core; and coating with kappa-carrageenan / konjac glucomannan / phytosterol ester composite gel, drying and packaging to obtain a finished product. The double essential oils, the chitosan and the theabrownin are combined to form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; porous silicon dioxide actively maintains low water activity of particles to prevent lactic acid bacteria from being recovered in advance; the product has the advantages of high viable count, strong heat and acid resistance and excellent storage stability.

The invention discloses a freeze-dried powder base containing a bionic sebum composition and a preparation method of the freeze-dried powder base, and belongs to the technical field of cosmetics. The freeze-dried powder substrate comprises a freeze-dried powder phase and a liquid matrix phase in a weight ratio of 1: (3-6), the freeze-dried powder phase is composed of a phase A, a phase B, a phase C and a phase D, the phase C contains a bionic sebum composition, and the composition is formed by compounding ceramide, a phytosterol ester compound and squalane according to a specific weight ratio; the liquid matrix phase comprises a water phase and an oil phase, and the components of the phases are proportioned according to specific weight percentages. Through the cooperation of the bionic sebum composition and the freeze-drying process, the foundation can quickly reconstruct a human sebum-like film, skin cutin gaps are filled, and the technical problems that a traditional foundation make-up is poor in skin attaching performance, prone to powder clamping and short in make-up lasting time are solved; and meanwhile, the freeze-drying process can stabilize functional components which are easy to inactivate at high temperature, so that the function of combining base makeup and care is realized.

The invention discloses a high-internal-phase emulsion with stable food-grade lipid eutectic Pickering particles as well as a preparation method and application of the high-internal-phase emulsion. The preparation method comprises the following steps: preparing lipid eutectic Pickering particles by taking high-melting-point lipid and phytosterol ester as a lipid eutectic matrix and supplemented with an emulsifier aqueous solution, respectively taking the lipid eutectic Pickering particles as a continuous phase and vegetable oil as a dispersed phase, setting the volume fraction of the inner phase to be 75-85%, and setting the volume fraction of the outer phase to be 75-85%; the oil-in-water type high internal phase emulsion stabilized by food-grade lipid eutectic Pickering particles is prepared. Liquid drops of the high-internal-phase emulsion are arranged in a compact and extruded mode, the stability of the emulsion in hot processing is improved due to the existence of an interface eutectic particle layer, raw material composition protection is achieved, and grease oxidation and high-temperature bad product formation in hot processing are reduced. The invention provides a feasible way for constructing the high-internal-phase emulsion with the active eutectic interface layer, and is beneficial to wide application of the high-internal-phase emulsion in the hot-processing food industry.

The invention relates to the technical field of functional preparations, in particular to a composition for inhibiting activity of 5 alpha-reductase and application of the composition. The composition disclosed by the invention is prepared from the following components in parts by weight: 1 to 40 parts of lycopene, 80 to 400 parts of phytosterol and / or phytosterol ester and 0.7 to 3.3 parts of melia azedarach leaf evodia rutaecarpa cornus officinalis B. In the composition, all the components are mutually matched and supplement each other, the composition has a remarkable effect of inhibiting the activity of 5alpha-reductase, and androgen dependent diseases such as androgenetic alopecia, benign prostatic hyperplasia and the like can be effectively improved. The raw materials of the composition are all natural active components, so that the composition has good effects of improving benign prostatic hyperplasia and inhibiting androgenetic alopecia, is safe and effective, and has a wide application prospect.

The utility model relates to a separation and purification system of phytosterol ester, which comprises a pre-mixing module, an extraction module, a saponification module, a liquid separation module, an adsorption module, an evaporator and a dryer, the pre-mixing module is used for mixing and heating an extraction liquid A and an extraction liquid B, the extraction liquid A is insoluble in water and can dissolve the phytosterol ester, and the extraction module is used for extracting the phytosterol ester. The pre-mixing module is provided with an extraction liquid B, the extraction liquid B is dissolved in water and can dissolve phytosterol, a crude product to be separated and purified is added into the extraction module, a weak base solution is added into the saponification module, the adsorption module is filled with activated carbon, and the pre-mixing module, the extraction module, the saponification module and the adsorption module are micro-channel continuous flow modules. The device disclosed by the utility model has the beneficial effects that esterification crude products of fatty acid and phytosterol are separated by utilizing a modularized micro-channel continuous flow extraction technology, and the problem that sterol, fatty acid and sterol ester in the crude products coexist and are difficult to separate is solved, so that high-purity phytosterol ester is obtained.

The invention relates to the technical field of textile, and discloses a deerskin-imitated velvet sock and a preparation method thereof, and the method comprises the steps of sock blank weaving, dyeing treatment, preparation of temperature self-adaptive multi-element synergistic post-treatment liquid, padding treatment, drying and shaping, after-treatment and the like. Wherein the post-treatment liquid is composed of seven components including a phospholipid compound, sodium alginate, xanthan gum, fatty alcohol-polyoxyethylene ether, a lanolin derivative, medium-chain triglyceride and phytosterol ester in a synergistic manner, and a double-layer structure including an elastic bottom layer and a soft surface layer is formed on the surface of the fiber; the medium-chain triglyceride and the phytosterol ester respectively provide low-temperature anti-freezing performance and high-temperature stability performance, so that temperature self-adaptive regulation and control are realized. The method solves the technical problems of insufficient suede fullness, easy flock lodging, non-uniform treatment and poor temperature adaptability of the existing imitation deerskin velvet socks, and enables the socks to keep stable suede effect and soft hand feeling in a wide temperature range of-5 DEG C to 45 DEG C.

This invention discloses a liposomal curcumin composition for regulating cardiovascular and cerebrovascular health and its application, belonging to the field of food and health food technology. The core components of the composition include: 60-80nm liposomal curcumin, prebiotics (2-8g / serving), cardamom extract (10-60mg / serving), animal brain-derived neurotrophic microcapsule powder (200mg daily), and one or more of the following: nattokinase, phytosterol esters (phytosterol content ≥90%, 500-2000mg / serving), coenzyme Q10, and hawthorn extract (total flavonoids ≥5%, 100-400mg / serving). This invention constructs a five-fold protective system through the synergistic effect of multiple components, encompassing intestinal regulation, blood purification, vascular care, myocardial enhancement, and brain nerve nourishment, achieving integrated regulation of cardiovascular and cerebrovascular health. Its bioavailability is more than 12 times higher than that of ordinary curcumin, and it achieves a comprehensive improvement rate of 86.7% for sub-health conditions of cardiovascular and cerebrovascular health in middle-aged and elderly individuals. It can be prepared as an oral liquid, solid beverage, soft capsule, or tablet, possessing extremely high application value.

The invention discloses a warm tonifying type food health-care formula capable of replenishing qi to invigorate the spleen and a preparation method, the formula comprises tremella, lily, jujube juice, passion fruit juice, walnut kernels, mung beans and xylitol, and further comprises fructo-oligosaccharide, konjaku flour, phytosterol ester and a proper amount of purified water, and the processing method comprises the following steps: cleaning fresh tremella, adding the purified water, keeping for 1520 minutes, and filtering to obtain filtrate; the preparation method comprises the following steps: cleaning 20% of Chinese dates, heating the Chinese dates to 100 DEG C in purified water according to 15 times of the amount of the Chinese dates, and keeping for 60-80 minutes until the Chinese dates are boiled to be broken but not broken and have no sweet taste; crushing lily into particles and steaming; cooking the mung beans into porridge in purified water; crushing the walnut kernels into particles with the diameter of about 5mm; dissolving xylitol with 10 times of purified water; mixing the treated tremella, lily, walnut kernel and mung bean with the jujube juice, the passion fruit juice, the xylitol solution and the jujube juice, and adding purified water to adjust to be atherosclerotic; the steamed bun is palatable and delicious in taste, low in nutrition loss, quick to heat, convenient and quick to eat and convenient to carry, and can also be eaten cold.

The application provides a phytosterol ester emulsion and a preparation method and application thereof, and the method comprises the following steps: S1, respectively preparing an emulsifier solution and a stabilizer solution, the emulsifier is sodium caseinate or egg albumin, the mass concentration of the emulsifier solution is 0.5-2%, and the stabilizer is high methoxyl pectin, sodium carboxymethyl cellulose or propylene glycol alginate, and the mass concentration of the stabilizer solution is 0.5-2%; S2, mixing the emulsifier solution with phytosterol ester, the mass ratio of the emulsifier solution to the phytosterol ester is 80:20-95:5, and the intermediate emulsion is obtained through high-speed shearing and high-pressure homogenization; and S3, mixing the intermediate emulsion with the stabilizer solution, the mass ratio of the intermediate emulsion to the stabilizer solution is 1:2-2:1, adjusting pH, and high-speed shearing to obtain the phytosterol ester emulsion. The application improves the dispersibility and stability of phytosterol ester in water, so that the phytosterol ester can be conveniently applied in liquid beverages, and the phytosterol ester has high stability and bioavailability.

The invention discloses a total nutrient compound based on nutrition and metabolism coordinated regulation and a preparation method thereof. The compound comprises a three-level function coordinated system, namely a nutrition foundation system, a metabolism regulation core system and a metabolism environment guarantee system. The nutrition foundation system comprises a slow-release energy supply unit and a basic nutrition enhancement and anti-oxidation unit; the metabolism regulation core system comprises a glycolipid metabolism regulation unit and an intestinal micro-ecological regulation unit; the glycolipid metabolism regulation unit consists of organic trivalent chromium, organic magnesium and organic selenium; the intestinal micro-ecological regulation unit is composed of a psyllium husk extract and micro-capsule embedded composite probiotics; the metabolic environment guarantee system comprises a nerve-endocrine homeostasis unit and a blood vessel health guarantee unit; the nerve-endocrine homeostasis unit consists of a South African drunken solanum extract, L-theanine and gamma-aminobutyric acid; the blood vessel health guarantee unit consists of nattokinase, phytosterol ester, a grape seed extract and a deep sea fish oil extract.

The present application relates to a kind of novel anti-freezing anti-cracking hand cream, according to percentage by weight, including, water phase:55~65%, oil phase:28~38%, emulsification-stable system:3~6%, active repair phase:2~4%, buffer-preservation-fragrance:0.3~0.8%;Wherein, water phase includes, bamboo leaf oligosaccharide:5~10%, 1,3-propanediol:8~12%, erythritol:3~6%, purified water: remainder to water phase 100%;Oil phase includes, camellia seed oil:8~12%, mango seed fat:6~10%, coconut oil MCT:4~8%, phytosterol ester:2~4%, rice bran wax microparticle:2~4%;Emulsification-stable system includes, sucrose stearate:2~3.5%, hydrogenated lecithin:1.5~2.5%, sphingomonad fermenting polysaccharide:0.3~0.7%.The present application also relates to a kind of novel anti-freezing anti-cracking hand cream preparation process.The present application gives quantifiable systematic breakthrough to the current hand cream "thick heavy stuff, high temperature inactivation, low temperature failure, poor wash resistance, slow repair, non-environmental protection " six structural pain points: absorption is fast, extrusion force is small, wash resistance is prolonged, and fissured healing is fast.

The application belongs to the technical field of food engineering, and particularly relates to a food-grade emulsion enzyme catalysis system, a construction method and application in functional lipid synthesis. The food-grade Pickering emulsion is formed by taking water-insoluble protein-chitin nanoparticles and a lipase CRL compound as the water phase and taking a reaction substrate fatty acid as the oil phase, and is used for preparing phytosterol esters. The water-insoluble protein-chitin nanoparticles have excellent dispersibility, stability and high surface positive charge, the lipase is adsorbed on the surface of the nanoparticles through electrostatic and hydrogen bond interaction, has reduced interfacial tension and enhanced interfacial activity, the lipase in the Pickering emulsion stabilized by the nanoparticles is distributed on the oil-water interface, the catalytic efficiency of the lipase is effectively improved, and the food-grade Pickering emulsion interface catalysis is used to efficiently synthesize phytosterol esters.

The invention relates to the technical field of feed additives, in particular to a feed regulating agent for reducing egg cholesterol, which comprises the following components in parts by mass: 8-12 parts of phytosterol ester; 5 to 8 parts of chitosan oligosaccharide; 15 to 20 parts of soybean lecithin; 3-5 parts of tea polyphenol; 3-5 parts of choline chloride; 2 to 4 parts of soy isoflavone; 5-10 parts of inulin; 1 to 2 parts of inositol; 2 to 3 parts of compound probiotics; and 30-50 parts of a carrier. According to the scheme, the cholesterol is further reduced by about 10% compared with the prior art by utilizing a cholesterol absorption mechanism from the perspective of blocking cholesterol absorption through component synergy, and the cholesterol content is only about 60% of that of normally bred and produced eggs.

The application relates to a multi-zone temperature control biological reaction equipment for improving phytosterol ester synthesis transmission efficiency, and relates to the technical field of synthesis equipment, which comprises a reaction bucket, two partition plates are arranged on the inner wall of the reaction bucket, the reaction bucket is sequentially divided into a first reaction cavity, a second reaction cavity and a third reaction cavity by the two partition plates, a first motor is fixedly installed at the top of the reaction bucket, and a central shaft is fixedly connected to the output end of the first motor. The self-rotation movement of the plurality of overturning blades fixedly connected to the bevel gear side is driven, the precipitate after scraping is driven to be lifted upwards, the phytosterol ester precipitate is beneficial to being brought to the central position of the third reaction cavity, mixing with liquid phytosterol ester is realized, the lifted phytosterol ester precipitate is gradually restored to liquid state, the phytosterol ester precipitation is reduced, the yield and the yield of phytosterol ester synthesis are improved, and the synthesis transmission is facilitated.

The invention relates to the technical field of food, and particularly discloses a composition for improving hearing of middle-aged and elderly people and application. The composition for improving hearing of middle-aged and elderly people provided by the invention comprises the following components: phytosterol ester, colostrum basic protein and N-acetylglucosamine. The composition for improving hearing of the middle-aged and elderly people can improve age-related hearing loss through ear-intestine axis bidirectional adjustment. According to the composition for improving hearing of the middle-aged and elderly people, through the synergistic effect of all the components, the abundance of beneficial bacteria bifidobacterium in intestinal tracts can be improved, the abundance of enterobacter can be reduced, the auditory brainstem reflection threshold value under stimulation of different frequencies (4 kHz to 40 kHz) can be relieved, meanwhile, the oxidative stress process is inhibited, and the composition can be used for preparing products for improving hearing of the middle-aged and elderly people.

This invention relates to the field of functional product technology, and more particularly to a composition for assisting in lowering blood lipids and its application. The invention provides a composition comprising *Bifidobacterium animalis* subsp. *lactamase* MN-Gup, microalgae oil, and phytosterol esters. The composition of this invention can simultaneously and effectively reduce triglyceride and total cholesterol levels, particularly significantly reducing low-density lipoprotein cholesterol while increasing high-density lipoprotein cholesterol levels. Compared to each individual component, it exhibits significant advantages, demonstrating significant auxiliary lipid-lowering efficacy with good safety and industrial feasibility. This provides a basis for the development of auxiliary lipid-lowering products and offers a new intervention strategy for blood lipid health management.

The application discloses a preparation method and application of a hollow hierarchical porous MOF material, and successfully generates the hollow hierarchical porous MOF material with a hollow hierarchical porous core-shell structure by adopting a combined strategy of PVP surface modification and Tris-HCl buffer etching, and the structural integrity of the hollow hierarchical porous MOF material is maximally maintained in a water-involved condition as a carrier for an enzyme molecule encapsulation process. In addition, the hollow hierarchical porous metal organic framework is modified by 3-aminopropyltrimethoxysilane, and higher enzyme loading efficiency is achieved. The experimental results show that the maximum enzyme loading capacity is 216.3 mg / mL, the relative activity of the immobilized enzyme is increased by 15 times compared with the CRL@ZIF-8 activity, and the immobilized enzyme has good thermal, chemical and operation stability. The immobilized enzyme is used for catalyzing synthesis of phytosterol ester, and the maximum conversion rate is 88.8%, and the immobilized enzyme shows excellent catalytic performance. The application provides a new idea for developing the MOF immobilized enzyme for biotechnological applications.