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Method for preparing functional grease rich in phytosterol ester and diglyceride

A technology of phytosterol ester and diglyceride, which is applied in biochemical equipment and methods, oil/fat refining, edible oil/fat, etc., to achieve the effects of simple operation, non-oxidation, and simple equipment

Active Publication Date: 2013-10-16
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of functional oils rich in sterol esters and diglycerides with two nutritionally active ingredients by one-step enzyme catalysis has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of a functional oil rich in phytosterol esters and diglycerides, comprising the steps of:

[0022] (1) Pretreatment of raw materials: the reaction solvent (n-hexane) was dehydrated with anhydrous sodium sulfate (moisture <0.1 wt%), sitosterol (phytosterol) was vacuum-dried at 80°C for 12 hours (moisture <1 wt%), triglyceride The ester adopts refined linseed oil (moisture <0.2 wt%);

[0023] (2) Addition of reaction raw materials: Add 5L of n-hexane, 207g of phytosterols (100mM), 880g of linseed oil (200mM), and molecular sieves (50g / L, ie 250g) into the reaction kettle, heat to 50°C and stir Homogeneous (stirring time is 30 minutes) to obtain a premix;

[0024] (3) Non-aqueous enzymatic transesterification reaction: Add 25g (5g / L) Candida antarctica lipase, add natural vitamin E antioxidant 200ppm, stir and react at 50°C for 12h under normal pressure, stop heating, and cool naturally to room temperature;

[0025] (4) Post-treatment of the product...

Embodiment 2

[0027] A preparation method of a functional oil rich in phytosterol esters and diglycerides, comprising the steps of:

[0028] (1) Pretreatment of raw materials: the reaction solvent (isooctane) was dehydrated with anhydrous sodium sulfate (moisture <0.1 wt%), stigmasterol (phytosterol) was vacuum-dried at 100°C for 10 hours (moisture <1 wt%), glycerol The triester adopts refined rapeseed oil (moisture<0.2 wt%);

[0029] (2) Addition of reaction raw materials: Add 5L isooctane, 310.5g phytosterol (stigmasterol 150mM), 2616g (600mM) rapeseed oil, and molecular sieve (addition amount is 50g / L, ie 250g) into the reaction kettle, heat Stir evenly at 55°C (stirring time is 30 minutes) to obtain a premix;

[0030] (3) Non-aqueous enzymatic transesterification reaction: add 50g (10g / L) Candida antarctica lipase, add fat-soluble tea polyphenol antioxidant 150ppm, stir and react at 55°C for 10h, stop heating, and naturally Cool to room temperature.

[0031] (4) Post-treatment of the...

Embodiment 3

[0033] A preparation method of a functional oil rich in phytosterol esters and diglycerides, comprising the steps of:

[0034] (1) Pretreatment of raw materials: the reaction solvent (n-heptane) was dehydrated with anhydrous sodium sulfate (moisture <0.1 wt%), brassicasterol was vacuum-dried at 120°C for 8 hours (moisture < wt%), triglycerides were refined sunflower oil (moisture <0.2 wt%);

[0035] (2) Adding the reaction raw materials: Add 5L of n-heptane, 414g of phytosterols (brassicasterol 200mM), 5232g of sunflower oil (1200mM), and molecular sieves (the addition amount is 50g / L, that is, 250g) into the reaction kettle, and heat Stir evenly at 65°C (stirring time is 30 minutes) to obtain a premix;

[0036] (3) Non-aqueous enzymatic transesterification reaction: Add 200g (20g / L) Candida lipolytica lipase, add L ascorbyl palmitate antioxidant 200ppm, stir and react at 65°C for 12h under normal pressure, stop heating, Naturally cool to room temperature.

[0037] (4) Post...

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Abstract

The invention relates to a method for preparing functional grease rich in phytosterol ester and diglyceride. The method is characterized by comprising the following steps of: (1) adding reaction raw materials: adding phytosterol, triglyceride, a reaction solvent and molecular sieves into a reaction kettle in a manner that the mole ratio of the phytosterol to the triglyceride is 1: 2 to 1: 6, the proportioning ratio of the phytosterol to the reaction solvent is 100-200mmol: 1L, and the added amount of the molecular sieves is 50 g / L, thereby obtaining a premix; (2) carrying out non-aqueous enzymatic transesterification reaction: adding 5-20 g / L of lipase into the premix, adding 100-200ppm of antioxidant, and carrying out stirring reaction for 8-12 h at the temperature of 50-65 DEG C and normal pressure; and (3) carrying out after-treatment on a product: after the reaction is completed, carrying out centrifugation so as to remove the lipase and the molecular sieves, and carrying out rotary evaporation under reduced pressure so as to remove the reaction solvent, thereby obtaining the functional grease rich in phytosterol ester and diglyceride. The method has the advantages that the functional grease rich in two nutritional active ingredients can be obtained through one-step reaction, the product does not need separation and purification, and the operation is simple and convenient.

Description

technical field [0001] The invention relates to a preparation method of functional oil rich in phytosterol ester and diglyceride, in particular to a method for synthesizing functional oil rich in phytosterol ester and diglyceride in one step through a non-aqueous enzyme-catalyzed transesterification method. Background technique [0002] Studies have shown that phytosterol esters obtained by esterification modification of phytosterols can not only significantly improve their own fat solubility, thereby expanding their application range in oil or oil-containing foods. In addition, the absorption and utilization rate of phytosterol ester is 5 times that of phytosterol, and it has better lipophilicity and better cholesterol-lowering effect. It is a new type of functional food base material. In 2010, phytosterol esters were listed as new food resources by the Ministry of Health of my country. Diacylglycerol is a structural oil formed by combining two molecular fatty acids to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02C12P7/64
CPCC12P33/00C12Y301/01003C12P7/6436A23D9/007C11B3/00C11B3/003C11B3/12C11C3/04A23D9/02
Inventor 黄凤洪郑明明黄清郭萍梅李文林邓乾春杨湄万楚筠钮琰星黄庆德刘昌盛许继取
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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