Method for effectively synthesizing phytosterol ester

A technology of phytosterol ester and synthesis method, which is applied in the field of high-efficiency synthesis of phytosterol ester, can solve the problems of harsh operating conditions and low catalyst safety, and achieve the effects of not easy oxidative denaturation, less by-products, and high product safety

Inactive Publication Date: 2010-09-29
JIANGNAN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to develop a method for efficiently synthesizing phytosterol esters for the shortcomings of low catalyst safety and harsh operating conditions in the current phytosterol ester synthesis process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the synthesis of phytosterol laurate

[0019] Take by weighing 0.8g of mixed phytosterols and 0.8g of lauric acid in a 50mL Erlenmeyer flask with a stopper, add 1% free lipase (derived from Rhizopus Aspergillus niger, activity 700000U / g) of the total mass of phytosterols and lauric acid raw materials as a catalyst, Add 16 mL of n-hexane, control the water activity at 0.41, and react at a constant temperature of 45°C for 24 hours. The obtained reaction product is separated and purified to obtain phytosterol laurate with a purity of 95%.

Embodiment 2

[0020] Embodiment 2: the synthesis of phytosterol docosahexaenoate

[0021] Weigh mixed phytosterol 0.8g, docosahexaenoic acid (DHA) 4.0g in 50mL Erlenmeyer flask with stopper, add phytosterol and docosahexaenoic acid raw material gross mass 10% immobilized lipase (source In Candida, activity 10000U / g) as a catalyst in the above-mentioned Erlenmeyer flask with a stopper, add 20 mL of mixed solvent of n-hexane: cyclohexane: isooctane=6: 2: 1 (v / v) in the Erlenmeyer flask . Control the water activity at 0.87, and react at a constant temperature of 40°C for 96h. The obtained reaction product is separated and purified to obtain phytosterol docosahexaenoate with a purity of 92%.

Embodiment 3

[0022] Embodiment 3: the synthesis of phytosterol fatty acid ester

[0023] Weigh 0.8 g of mixed phytosterols and 8 g of mixed fatty acids in a 50 mL Erlenmeyer flask with a stopper. First add phytosterols and 5% free lipase (derived from Rhizopus, activity 30000U / g) of the total mass of mixed fatty acid raw materials as a catalyst in the above-mentioned Erlenmeyer flask with a stopper, and add 5 mL of solvent n-heptane in the Erlenmeyer flask. Control the water activity to 0.62, and after 24 hours of constant temperature reaction at 55°C, change the water activity of the system to 0.12, then add 3% immobilized lipase (derived from Aspergillus niger, 50000U / g) of the total mass of raw materials, and continue the reaction for 24 hours. The obtained reaction product is separated and purified to obtain a phytosterol fatty acid ester with a purity of 96%.

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Abstract

The invention relates to a method for effectively synthesizing phytosterol ester, belonging to the technical fields of food, medicine and cosmetics. The method has the technical scheme that the high-purity phytosterol ester is synthesized by the steps of directly mixing phytosterol and fatty acid, adding biocatalyst and organic solvent for reaction at the temperature of 35-55 DEG C for 24-96h, and controlling the water activity of a reaction system to be 0.1-0.9. By adopting a biological method to prepare the phytosterol ester, the method has high-efficiency and green processing technique, safety and high conversion rate, and the obtained product has the functional characteristics of reducing cholesterol, preventing and curing cardiovascular and cerebrovascular diseases, antioxidation and the like, so that the invention can be widely applied to multiple fields such as food, medicine, cosmetics and the like.

Description

technical field [0001] The invention relates to a method for efficiently synthesizing phytosterol esters, in particular to a method for efficiently synthesizing high-purity phytosterol esters by lipase in a non-aqueous phase system. The application development of the product of the invention relates to the technical fields of food, medicine and cosmetics. Background technique [0002] With the continuous improvement of living standards, the incidence of cardiovascular and cerebrovascular diseases is increasing. A large number of studies have shown that the high concentration of cholesterol in the blood, especially the high concentration of low-density lipoprotein cholesterol, is the main factor causing various cardiovascular and cerebrovascular diseases. Reducing the concentration of cholesterol in the blood can significantly reduce the incidence of cardiovascular and cerebrovascular diseases. Therefore, the current dietary and drug treatments for these diseases all start ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/685C12R1/72C12R1/845
Inventor 江波缪铭刘虹蕾
Owner JIANGNAN UNIV
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