Enzymatic synthesis method of phytosterol ester/phytostanol ester by utilizing ultrasonic enhancement

A technology of phytostanol esters and phytosterol esters, which is applied in the field of synthesis of sterol esters, can solve problems such as few reports, and achieve the effects of high total reaction yield, increased concentration, and improved catalytic activity

Active Publication Date: 2012-08-01
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are not many reports on ultrasound-assisted enzymatic esterification, and there is no public information on the application of ultrasound in the enzymatic synthesis of sterol esters / phytostanol esters.

Method used

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  • Enzymatic synthesis method of phytosterol ester/phytostanol ester by utilizing ultrasonic enhancement
  • Enzymatic synthesis method of phytosterol ester/phytostanol ester by utilizing ultrasonic enhancement

Examples

Experimental program
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Effect test

Embodiment 1

[0029] like figure 1 Shown, a kind of method that utilizes ultrasonic strengthening enzymatic synthesis phytosterol ester, it comprises the steps:

[0030] 1) Prepare heterogeneous enzyme reaction system: Weigh 413g (1M) phytosterol (stigmasterol), add 417g (1.5M) α-linolenic acid into the reaction kettle, and then add 10L isooctane (at this time, the concentration of phytosterol is 100mmol / L), add 150g of Candida lipolytica lipase, the amount of molecular sieve added is 600g, the temperature of the solution is heated to 55°C, and fully mixed by mechanical stirring.

[0031] 2) Ultrasound-assisted enzymatic esterification (enzymatic esterification reaction system): keep the temperature of the reaction system at 55°C, stir and mix evenly at a speed of 100rpm, immerse the ultrasonic probe in the reaction solution, turn on the ultrasonic generator, and adjust the frequency to 20Hz. The power is 100W, and the mechanical stirring is stopped after 40 minutes of ultrasonic irradiati...

Embodiment 2

[0034] A kind of method that utilizes ultrasound to strengthen enzymatic synthesis of phytosterol ester, it comprises the steps:

[0035] 1) Prepare heterogeneous enzyme reaction system: weigh 621g (1.5M) phytosterol (sitosterol), add 630g (2.25M) linoleic acid into the reaction kettle, then add 10L n-hexane (at this time, the concentration of phytosterol is 150mmol / L), the amount of molecular sieve added was 400g, and 100g of Candida antarctica immobilized enzyme (Novozym 435, Novozym (China) Biotechnology Co., Ltd.) was added.

[0036] 2) Ultrasound-assisted enzymatic esterification (enzymatic esterification reaction system): keep the temperature of the reaction system at 50°C, stir and mix evenly at a speed of 200rpm, immerse the ultrasonic probe in the reaction solution, turn on the ultrasonic generator, and adjust the frequency to 25Hz. The power is 200W, and the mechanical stirring is stopped after 1 hour of ultrasonic irradiation, and the mechanical stirring reaction i...

Embodiment 3

[0039] A kind of method that utilizes ultrasound to strengthen enzymatic synthesis of phytosterol ester, it comprises the steps:

[0040] 1) Prepare a heterogeneous enzyme reaction system: Weigh 800g (2M) phytosterol (camesterol), add 672g (2.4M) mixed fatty acids obtained by hydrolysis of sunflower oil into the reactor, and then add 10L isooctane (at this time The concentration of phytosterol is 200mmol / L), 80g of Candida rugosa enzyme is added, the amount of molecular sieve added is 200g, the temperature of the solution is heated to 55°C, and fully mixed by stirring.

[0041] 2) Ultrasound-assisted enzymatic esterification (enzymatic esterification reaction system): keep the temperature of the reaction system at 55°C, stir and mix evenly at a speed of 200rpm, immerse the ultrasonic probe in the reaction solution, turn on the ultrasonic generator, adjust the frequency to 30kHz, The power is 300W, stop after 1.5h of mechanical stirring and ultrasonic irradiation, continue stir...

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Abstract

The invention relates to a preparation method of sterol ester and in particular relates to an enzymatic synthesis method of phytosterol ester / phytostanol ester by utilizing ultrasonic enhancement. The method is characterized by comprising the following steps: 1) preparing a heterogeneous enzyme reaction system: weighing the phytosterol ester / phytostanol ester and fatty acid at a molar ratio of 1:(1.2-2), adding a reaction solvent, and adding enzyme and a molecular sieve; 2) performing enzymatic esterification with ultrasonic assistance: immersing an ultrasonic probe in a reaction solution, opening an ultrasonic wave generator, constantly radiating with ultrasonic wave for 0.5 to 3 hours under mechanical stirring, and continuously reacting for 4 to 10 hours under mechanical stirring after the radiation is completed; and 3) removing the fatty acid with ultrasonic assisted saponification to obtain the product. According to the method, the enzymatic esterification reaction time can be remarkably shortened, the usage of enzyme can be reduced, and the saponification speed of excessive fatty acid can be accelerated, so that the efficiency of separation and purification can be improved, and the obtained phytosterol ester / phytostanol ester product has the characteristics of light color, high purity and safety without contamination.

Description

technical field [0001] The present invention relates to a method for synthesizing sterol esters, in particular to a method for synthesizing phytosterol esters / phytostanol esters through ultrasonic-assisted enhanced enzyme-catalyzed esterification, which is used to increase the speed of enzymatic esterification and the saponification of excess fatty acids removal efficiency. Background technique [0002] Studies have shown that phytosterol esters / phytostanol esters prepared by esterification of phytosterols and fatty acids can greatly increase the fat solubility of phytosterols / phytostanol esters, so that they can be added to oils or fats more easily. In fatty foods. In addition, the absorption and utilization rate of phytosterol ester is 5 times that of phytosterol, and it has better lipophilicity and better cholesterol-lowering effect. It is a new type of functional food base material. In 2010, phytosterol esters were listed as new food resources by the Ministry of Health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02
Inventor 黄凤洪郑明明郭萍梅李文林邓乾春杨湄黄庆德万楚筠钮琰星刘昌盛许继取杨金娥
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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