Enzymatic synthesis method of phytosterol ester/phytostanol ester by utilizing ultrasonic enhancement
A technology of phytostanol esters and phytosterol esters, which is applied in the field of synthesis of sterol esters, can solve problems such as few reports, and achieve the effects of high total reaction yield, increased concentration, and improved catalytic activity
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Embodiment 1
[0029] like figure 1 Shown, a kind of method that utilizes ultrasonic strengthening enzymatic synthesis phytosterol ester, it comprises the steps:
[0030] 1) Prepare heterogeneous enzyme reaction system: Weigh 413g (1M) phytosterol (stigmasterol), add 417g (1.5M) α-linolenic acid into the reaction kettle, and then add 10L isooctane (at this time, the concentration of phytosterol is 100mmol / L), add 150g of Candida lipolytica lipase, the amount of molecular sieve added is 600g, the temperature of the solution is heated to 55°C, and fully mixed by mechanical stirring.
[0031] 2) Ultrasound-assisted enzymatic esterification (enzymatic esterification reaction system): keep the temperature of the reaction system at 55°C, stir and mix evenly at a speed of 100rpm, immerse the ultrasonic probe in the reaction solution, turn on the ultrasonic generator, and adjust the frequency to 20Hz. The power is 100W, and the mechanical stirring is stopped after 40 minutes of ultrasonic irradiati...
Embodiment 2
[0034] A kind of method that utilizes ultrasound to strengthen enzymatic synthesis of phytosterol ester, it comprises the steps:
[0035] 1) Prepare heterogeneous enzyme reaction system: weigh 621g (1.5M) phytosterol (sitosterol), add 630g (2.25M) linoleic acid into the reaction kettle, then add 10L n-hexane (at this time, the concentration of phytosterol is 150mmol / L), the amount of molecular sieve added was 400g, and 100g of Candida antarctica immobilized enzyme (Novozym 435, Novozym (China) Biotechnology Co., Ltd.) was added.
[0036] 2) Ultrasound-assisted enzymatic esterification (enzymatic esterification reaction system): keep the temperature of the reaction system at 50°C, stir and mix evenly at a speed of 200rpm, immerse the ultrasonic probe in the reaction solution, turn on the ultrasonic generator, and adjust the frequency to 25Hz. The power is 200W, and the mechanical stirring is stopped after 1 hour of ultrasonic irradiation, and the mechanical stirring reaction i...
Embodiment 3
[0039] A kind of method that utilizes ultrasound to strengthen enzymatic synthesis of phytosterol ester, it comprises the steps:
[0040] 1) Prepare a heterogeneous enzyme reaction system: Weigh 800g (2M) phytosterol (camesterol), add 672g (2.4M) mixed fatty acids obtained by hydrolysis of sunflower oil into the reactor, and then add 10L isooctane (at this time The concentration of phytosterol is 200mmol / L), 80g of Candida rugosa enzyme is added, the amount of molecular sieve added is 200g, the temperature of the solution is heated to 55°C, and fully mixed by stirring.
[0041] 2) Ultrasound-assisted enzymatic esterification (enzymatic esterification reaction system): keep the temperature of the reaction system at 55°C, stir and mix evenly at a speed of 200rpm, immerse the ultrasonic probe in the reaction solution, turn on the ultrasonic generator, adjust the frequency to 30kHz, The power is 300W, stop after 1.5h of mechanical stirring and ultrasonic irradiation, continue stir...
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