88 results about "Structured lipid" patented technology

The invention discloses a pet food with a synergistic anti-inflammatory function and a preparation method thereof, and the method comprises the following steps: preparing an anti-inflammatory nano-carrier, preparing an anti-inflammatory synergistic emulsion, emulsifying and dispersing active ingredients, tempering, extruding, puffing and drying, preparing probiotic particles, performing step-by-step vacuum spraying and the like. And a multi-level active component protection and delivery system is systematically constructed. Wherein the nanostructured lipid carrier and the microencapsulated ginger active extract are compounded according to a specific proportion, and a synergistic interaction system is formed through a fat source medium; and the probiotics are sprayed and added after being subjected to a multi-layer embedding and low-temperature granulation technology. The pet food prepared by the method comprises an anti-inflammatory synergistic composite unit and a probiotic particle unit, and experiments prove that the bioavailability of the core anti-inflammatory component reaches 52%, the in-vitro TNF-alpha inhibition rate reaches 65%, and the survival rate of probiotics exceeds 4.2 * 10 < 8 > CFU / g, so that the pet food is obviously superior to the traditional process, and the synergistic improvement of the anti-inflammatory effect, the component stability and the processing adaptability is realized.

The invention discloses a multilevel liver targeting drug delivery system based on ionizable amino lipid and a preparation method thereof, the system comprises the following components: target modified ionizable amino lipid, ionizable amino lipid, auxiliary lipid and structural lipid in a molar ratio of (10-30): (30-60): (5-40): (15-55), according to the system, nano lipid particles exist in a water-in-oil-in-water nano emulsion form; wherein the ionizable ammonia lipid is a compound with a structure as shown in a formula (I). The system is suitable for delivering nucleic acid, protein and other active pharmaceutical ingredients to the liver so as to improve the accuracy and treatment effect of drug delivery and reduce the side effects of non-target organs.

The purpose of the present invention is to provide: lipid nanoparticles useful as a nucleic acid delivery carrier; and a novel pH-sensitive cationic lipid for producing the lipid nanoparticles. The problem is solved by: a pH-sensitive cationic lipid comprising an asymmetric hydrocarbon chain; and lipid nanoparticles comprising the pH-sensitive cationic lipid as a constituent lipid.

The invention relates to the field of synthetic biology, in particular to a structural lipid, a preparation method thereof, a structural lipid composition containing the structural lipid and used for protecting liver and eyes, and a product for protecting liver and eyes. The preparation method comprises the following steps: carrying out reaction on mixed oil and lipase, and carrying out enzyme deactivation to prepare structural lipid; wherein the weight ratio of omega3 fatty acid to omega6 fatty acid to omega9 fatty acid in the oil material is (0.8-1.2): (0.8-1.2): (0.8-1.2). The lipase is adopted to carry out hydrolysis reaction on appropriate oil, sn-2 site fatty acid with the liver and eye protection effect can be prepared, and the sn-2 site fatty acid is small in molecular weight and beneficial to absorption.

Provided herein are targeting compounds (e.g., a compound of Formula I, a stereoisomer thereof, a tautomer thereof, and / or a pharmaceutically acceptable salt thereof), lipid nanoparticle (LNP) compositions comprising such targeting compounds and the use thereof. The LNP compositions described herein may further comprise one or more selected from ionizable lipids, PEG-lipids, phospholipids, and structural lipids.

The invention belongs to the technical field of fat synthesis, and particularly discloses a method for synthesizing 1, 3-dilinoleic acid-2-palmitic acid triglyceride, which comprises the following steps: S1, dissolving palm stearate in a solvent, and purifying to obtain PPP; s2, mixing the PPP purified in the step S1 with LA, dissolving, and adding immobilized lipase ANL-MARE to carry out primary reaction; after the primary reaction is completed, carrying out acid removal; and then adding LA again to carry out secondary reaction, filtering to remove enzyme after the reaction is completed, and separating and purifying to obtain the LPL structure lipid. According to the method, PPP-rich palm stearin and linoleic acid are used as raw materials, and immobilized lipase is utilized to catalyze two-step acidolysis reaction in a solvent-free system to synthesize the structured triglyceride rich in LPL. Compared with a common one-step acidolysis method, the method has the advantages that the relative content of LPL is increased by 10.27%, the relative content of sn-2PA is kept unchanged, the usage amount of enzyme is effectively reduced, and the LPL efficiently synthesized through enzymatic catalysis of the method has the potential of being used for production of human milk fat substitutes.

A composition including ionizable lipids is provided. Methods of making the ionizable lipids are also provided. Also provided are compositions forming lipid nanoparticles, wherein the composition includes the ionizable lipid, a helper lipid, a structural lipid or sterol, and a polymer-conjugated lipid. Methods of using the ionizable lipid and lipid nanoparticles are also provided.

The invention provides a rucotinib phosphate nanostructure lipid carrier, a preparation method and application thereof, and a rucotinib phosphate composition, and belongs to the technical field of pharmaceutical preparations. According to the rucotinib phosphate nanostructure lipid carrier and the rucotinib phosphate composition provided by the invention, the solid lipid and the liquid lipid are compounded, and the anti-crystallization agent is introduced, so that the rucotinib phosphate is encapsulated in the nanostructure lipid carrier core formed by the solid lipid and the liquid lipid; the problem of unique crystallization caused by a pyrazole structure of rucotinib phosphate is solved, and the compound has the advantages of high encapsulation efficiency and good stability. According to the invention, the rucotinib phosphate nanostructure lipid carrier is uniformly dispersed in a dermatologically acceptable matrix to obtain a composition containing a therapeutically effective amount of rucotinib phosphate, and the composition shows significantly enhanced drug stability and skin retention ability, and reduces the risk of system exposure.

The present application relates to the technical field of nucleic acid drug preparation, and discloses a lipid nanoparticle based on asiatic acid and a preparation method and application thereof, the lipid nanoparticle is composed of ionizable cationic lipids, structural lipids, neutral lipids, PEG lipids and asiatic acid with a molar ratio of 10-85:20-60:1-30:0.1-10:0.1-50, and is used as a delivery carrier of nucleic acid drugs for delivering nucleic acid drugs. The present application innovatively uses natural product asiatic acid to partially replace the structural lipids (such as cholesterol) in the traditional LNP formula, significantly optimizes the micro morphology, and greatly enhances the transfection and expression efficiency of mRNA in vitro and in vivo. The mRNA vaccine based on the carrier can induce the body to produce high-titer specific antibodies and significantly enhance the cytotoxic T lymphocyte (CTL) killing activity. The LNP system performs outstandingly in the field of tumor immunoprevention and treatment, and shows superior conversion application potential and broad market prospects.

The invention belongs to the technical field of biochemical engineering, and particularly relates to a method for preparing medium-chain fatty glyceride through double-enzyme catalysis. The method provided by the invention comprises the following steps: firstly, catalyzing raw materials such as coconut oil to be completely hydrolyzed by utilizing lipase, and separating medium-chain fatty acid with the purity of more than or equal to 99.5% by adopting a molecular distillation cooling crystallization technology; then, high-purity fatty acid and glycerol are used as substrates, immobilized lipase is used as a catalyst, directional esterification is performed in a reactor provided with a dehydration membrane block, and the water activity of the system is controlled to be 0.1-0.3. The content of medium-chain fatty glyceride in the final product is higher than 98%, and the final product does not contain long-chain fatty acid impurities. The process is green and energy-saving, the catalyst can be recycled, the economical efficiency is good, and an innovative solution is provided for large-scale production of the high-purity structured lipid.

The invention relates to the technical field of progesterone sustained-release nanoparticle production, in particular to a progesterone sustained-release nanoparticle which comprises progesterone and further comprises solid lipid and liquid lipid according to the formula ratio of 1: 1.4: 1.2: 0.8. A preparation method of the progesterone sustained-release nanoparticle comprises raw material preparation of progesterone, solid lipid and liquid lipid and melt emulsification. The prepared progesterone lipid nanoparticles have high drug entrapment efficiency which can reach 77.48% at most, and have obvious drug sustained and controlled release performance, although the particle size of the nanoparticles is increased along with the increase of the content of liquid lipid-oleic acid in the lipid nanoparticles, the drug entrapment efficiency is improved, the in-vitro release of the drug is accelerated, and the progesterone lipid nanoparticles have good application prospects. According to the progesterone nanostructure lipid carrier, the drug content in the preparation process of the nanoparticles is improved, the drug encapsulation efficiency of the prepared progesterone nanostructure lipid carrier is reduced, but the drug loading capacity of the nanoparticles can be improved, and when the dosage reaches 20%, the drug loading capacity of the nanoparticles can reach 11.57%.

The invention discloses a diosmetin nanostructure lipid carrier, which is prepared from the following raw materials by weight: 5-10% of diosmetin; 50-70% of a lipid material; 8-20% of cholesterol modified by mannose; and 15-25% of an emulsifier, belonging to the field of biological medicines. By preparing the nanostructured lipid carrier, the stability of diosmetin is improved, the release speed of the drug in vivo is delayed, the blood concentration and bioavailability are improved, the lung targeting property of the drug is also improved, the cytotoxicity of the drug is reduced, and the nanostructured lipid carrier has a good application prospect in clinical treatment of non-small cell lung cancer.

The present invention discloses a production method of a structured lipid containing medium-chain or short-chain fatty acids, comprising: S1, mixing a raw material oil and a liquid lipase in an enzyme-oil micro-mixer M1 to form an emulsified oil containing the liquid lipase; S2, starting a feed pump P3, and after preheating medium-chain triglycerides or short-chain triglycerides through a second preheating pipe L2, feeding them into a reaction micro-mixer M2 to carry out hydrolysis and transesterification reactions with the emulsified oil, using a delay pipe L3 to extend the reaction time to make the reaction reach equilibrium, and then subjecting the reaction product after the reaction to high-temperature enzyme inactivation through a quenching pipe L4, and then releasing and flowing out through a back pressure valve BPV. The present invention uses a liquid lipase as a catalyst and adopts a microchannel reaction system for production, which improves the mass transfer efficiency between the lipase and the oil and has the advantages of high reaction efficiency, fast reaction speed and low production cost. It not only improves the mass transfer efficiency between the lipase and the oil, but also realizes the continuous production of the structured lipid containing medium-chain or short-chain fatty acids, and improves the production efficiency of the structured lipid containing medium-chain or short-chain fatty acids.

This invention discloses an oil-based nanostructured lipid carrier based on MLCT, its preparation method, and its application. The method includes mixing long-chain oil with decanoic acid triglyceride, adding immobilized lipase, vacuum drying, stirring, filtering, deacidification, and vacuum evaporation to obtain MLCT-structured lipids. Glyceryl monostearate is mixed with the MLCT-structured lipids and heated to form the oil phase. Trichosanthes kirilowii seed oil is mixed with ultrapure water and heated to form the aqueous phase. The aqueous and oil phases are then mixed, heated, ultrasonically broken up, and subjected to an ice bath to obtain the oil-based nanostructured lipid carrier. This invention replaces traditional small-molecule emulsifiers with natural oil bodies (OB) as emulsifiers, meeting consumer health needs while exhibiting excellent stability and safety; it also reduces the risk of chemically synthesized residues. Furthermore, the introduction of MLCT revolutionizes the digestibility of NLCs at the metabolic mechanism level. In vitro simulated digestion experiments show that the free fatty acid release rate is higher than 80%, providing a new solution for the development of functional food and drug delivery systems.

The invention discloses a method for making a sponge cake by completely replacing butter with oleogel, and belongs to the technical field of food processing. The butter in a traditional sponge cake formula is completely replaced with the oleogel according to the mass ratio of 100%. According to the oleogel, a pea protein-high methoxyl pectin electrostatic compound is used as a gel agent, a three-dimensional structured lipid network is constructed by regulating and controlling the pH value of a system and the concentration of pectin, a liquid oil phase is effectively fixed, and the oleogel has excellent structural stability, thixotropic restorability and thermal stability. In the stirring and baking process, the oleogel provides necessary mechanical support and keeps a batter bubble system and a cake tissue structure stable, so that the sponge cake which is ideal in specific volume, uniform in pore and soft in texture is obtained, and the quality of the sponge cake reaches the level equivalent to that of a traditional butter cake. Zero-trans and low-saturated-fat healthy fat replacement is achieved, meanwhile, the processing adaptability and sensory quality of the sponge cake are kept, and a new technical approach and application prospects are provided for development of healthy baked food.

The present application discloses PLPC-DB, an ultrapurified phospholipoproteomic composition derived from the supernatant of peripheral blood mononuclear cells (PBMCs). It comprises essential phospholipids, bioactive proteins, regulatory peptides, and intercellular signaling factors. The composition exceeds 99% purity through a multi-stage purification process combining high-speed centrifugation and selective ultrafiltration (1-50 kDa), followed by lyophilization. This process ensures structural stability for ≥24 months and inter-batch variability <2%. PLPC-DB supports reproducibility and molecular integrity in research and diagnostic settings. Key components include phosphatidylcholine and phosphatidylserine for membrane stabilization and intracellular signaling; structural and regulatory lipids for membrane biogenesis and immune-relevant components involved in antigen presentation, cytokine modulation, and membrane-associated signaling. These may include structurally defined molecules compatible with immunological evaluation in experimental and non-therapeutic settings. The composition is formulated for bioaccessible delivery via sublingual, endonasal, transdermal, and injectable routes, and is intended exclusively for non-therapeutic use in experimental and translational models.

The invention discloses a preparation method of a silybin nanostructure lipid carrier, which comprises the following steps: step 1, heating ethanol and silybin in a water bath, and uniformly stirring to obtain a silybin solution; dispersing a co-emulsifier in deionized water, and heating and stirring in a water bath to obtain a water phase; step 2, adding solid lipid, liquid lipid, a main emulsifier and a co-emulsifier into the silibinin solution, heating for melting, and uniformly stirring to obtain an oil phase; under the condition of constant-temperature stirring, transferring the oil phase into the water phase, mixing the solution, and shearing to obtain a coarse dispersion system; and 3, heating the coarse dispersion system to concentrate the coarse dispersion system into an emulsion. The silibinin nanostructure lipid carrier prepared by the invention can improve the solubility of silibinin, and is good in characterization performance; the preparation cost is controllable, and macro preparation can be realized; the silybin nano-structure lipid carrier can be used for effectively preventing acute liver injury caused by CCl4 of animals.

The invention relates to the technical field of nucleic acid medicine preparations, and discloses lipid nanoparticles based on asiatic acid and a preparation method and application thereof.The lipid nanoparticles are composed of ionizable cationic lipid, structural lipid, neutral lipid, PEG lipid and asiatic acid according to the molar ratio of (10-85): (20-60): (1-30): (0.1-10): (0.1-50) and serve as delivery carriers of nucleic acid medicine. For delivery of nucleic acid drugs. According to the invention, a natural product asiatic acid is creatively adopted to partially replace a structural lipid (such as cholesterol) in a traditional LNP formula, and the micromorphology of the LNP is obviously optimized, so that the in-vivo and in-vitro transfection and expression efficiency of mRNA is greatly enhanced. The mRNA vaccine constructed based on the vector can induce an organism to generate a high-titer specific antibody, and the killing activity of cytotoxic T lymphocytes (CTL) is remarkably enhanced. The LNP system has outstanding performance in the field of tumor immunoprophylaxis and treatment, and shows excellent transformation application potential and wide market prospect.

The application provides a kind of detection method and application of multiple functional structural lipids in infant biscuit, belong to food detection technical field. Including: after sample is pulverized, using n-hexane-isopropyl alcohol-methanol-1,4-dioxane mixed extraction liquid extraction, obtain crude extract; Using modified graphene / silica gel composite adsorbent for solid phase extraction purification, obtain the test solution; Using high performance liquid chromatography-double detector combination system for detection, wherein C18 chromatographic column is combined with optimized gradient elution program to realize baseline separation of three C52 structural lipids, separation degree is greater than or equal to 1.5, ultraviolet detector and evaporation light scattering detector are connected in series respectively for determination of high concentration and low concentration target. The application realizes simultaneous detection of OPO, OPL and LPL three functional structural lipids in infant biscuit for the first time, has the advantages of comprehensive detection material, complex applicable matrix, excellent separation effect, high sensitivity, simple operation and the like.

Provided is a lipid nanoparticle, which comprises an active component, an ionizable lipid, an auxiliary lipid, a structural lipid, and a polymer lipid. The polymer lipid accounts for 0-0.8 mol% of the total lipid existing in the lipid nanoparticle. The reduction in the content of the polymer lipid in the lipid nanoparticle can change the delivery specificity of the lipid nanoparticle, such that the lipid nanoparticle can target the spleen or immune cells.

Provided herein are targeting compounds (e.g., a compound of Formula I, a stereoisomer thereof, a tautomer thereof, and / or a pharmaceutically acceptable salt thereof), lipid nanoparticle (LNP) compositions comprising such targeting compounds and the use thereof. The LNP compositions described herein may further comprise one or more selected from ionizable lipids, PEG-lipids, phospholipids, and structural lipids.

The invention discloses immobilized lipase, a preparation method and application of the immobilized lipase in preparation of structural lipid. The preparation method comprises the following steps: synthesis of an immobilized carrier, immobilization of enzyme and synthesis of the structural lipid. The lipase is immobilized by the nano mesoporous silica material, so that the reaction time is greatly shortened, the enzyme activity is better reserved, the use frequency of the enzyme is increased, and the problems of high cost and low utilization rate of the lipase in the industrial production process of sterol ester are solved. Two beneficial substances of sterol ester and diglyceride are synthesized at the same time through a one-step reaction, and the product can be directly used as functional grease without purification.

The present invention relates to a material for local drug delivery and use thereof. Specifically provided is a nanoparticle composition. The nanoparticle composition comprises a cationic lipid, a PEG lipid, and a structural lipid, wherein the lipid component of the PEG lipid is 1.0-5.5 mol%, or a range between any two of the described values. The nanoparticle composition of the present invention is delivered only at the site of administration.

The present invention discloses an enzymatic synthesis method of 1-palmitic acid-2-oleic acid-3-triglyceride stearate, belonging to the technical field of structured lipid processing. In the present invention, a crude product rich in 1-palmitic acid-2-oleic acid-3-triglyceride stearate is obtained by reacting for 2-10 h at 20-70° C. with 1-palmitic acid-2-diglyceride oleate as an acyl acceptor, stearic acid or a derivative thereof as an acyl donor and a lipase as a catalyst, wherein the reaction system is an inorganic or organic solvent reaction system. The present invention provides a highly efficient enzymatic synthesis method of POS, thereby resolving the disadvantage of low POS content and limited use provided by the current synthesizing product of structured lipid. The technology adopted by the present invention certainly has an application prospect in the process of preparing cocoa butter equivalents.

The invention discloses a nano-structure lipid carrier composite hydrogel capable of slowly releasing lemon fragrance and a preparation method of the nano-structure lipid carrier composite hydrogel. The method comprises the following steps: preparing a nano-structure lipid carrier containing lemon fragrance by adopting a hot melting ultrasonic method, adding the nano-structure lipid carrier into carboxymethyl chitosan / sodium alginate hydrogel, and incubating in a water bath at 37 DEG C to obtain the composite hydrogel. The lemon aroma component nanostructure lipid carrier composite hydrogel is prepared into different forms to be applied to the acid beverage. The nano-structure lipid carrier composite hydrogel provided by the invention has good acid stability and realizes good controlled release of lemon aroma substances.

Disclosed herein are novel lipids that can be used in combination with other lipid components, such as helper lipids, structural lipids, and cholesterols, to form lipid nanoparticles for delivery of therapeutic agents, such as nucleic acids (e.g., circular polynucleotides), both in vitro and in vivo.

The application discloses an anti-wrinkle and firming composition suitable for multiple skin types and a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition contains, in mass parts, 2.5-9.5 parts of an active functional component, 5-10 parts of a targeted delivery carrier system and cosmetically acceptable adjuvants. The active functional component is composed of ginsenoside Rb1, green tea extract-white resveratrol compound and asiaticoside-dipotassium glycyrrhizinate compound, and the targeted delivery carrier system is a transdermal peptide modified liposome and a natural nano-structured lipid carrier. The application constructs a double-targeted delivery system according to different skin aging mechanisms and skin characteristics, realizes single formula adaptation to multiple skin types, improves the transdermal efficiency of active ingredients and the targeted enrichment effect on the dermis, has no carrier residue, and multiple components synergistically play the effects of anti-wrinkle and firming, soothing and protection, is safe, and can be widely applied to the preparation of anti-aging skin care products.

This invention, in some embodiments thereof, provides a core- shell nanoparticle including a lipid-PSMA conjugate, encapsulating a liquid oil-based core. The invention further provides aqueous compositions including the core-shell nanoparticles, and methods for using same, such as for the treatment of proliferative diseases associated with PSMA upregulated expression.

The invention discloses an emulsion type suppository practical for vaginal administration, and relates to the technical field of pharmaceutical preparations, the suppository is a solid at room temperature, and can be converted into a cream type preparation in situ under vaginal temperature and wetting conditions; the suppository is composed of active and pharmaceutically acceptable auxiliary materials, the auxiliary materials comprise structural lipid, an emulsifier, a gel matrix and a pH regulator, and a suppository body is softened at body temperature and mixed with body fluid to form a cream mixture easy to coat on the mucous membrane surface in situ. The transformation process avoids greasy feeling and local discomfort possibly brought by a traditional greasy suppository, and also eliminates the trouble that the prefabricated cream needs to be matched with a special administration device, so that the local medication experience of a patient is improved while the administration convenience is ensured. Besides, through reasonable selection of auxiliary materials, the cream formed through conversion can provide proper adhesion, and the retention time of the medicine at the action part can be prolonged.