1 results about "Dideoxynucleotide" patented technology

This invention relates to a PAP-liquid phase chip and a high-throughput, high-specificity detection method for gene mutations. The chip contains primers and probes. The primers include forward and reverse primers. The 5' end of the forward primer is biotin-labeled or fluorescently labeled, and the 3' ends of both the forward and reverse primers are mutation-specific nucleotides, with the last nucleotide being a dideoxynucleotide. The 5' end of the probe has an amino group, followed by an 18-25 bp spacer arm, and then a 15-30 bp specific probe containing the mutation site. The probe is covalently linked to a magnetic sheet. The chip combines PAP technology with digital liquid phase chip technology, enabling the detection of rare mutations at a single point in a single tube, as well as the detection of multiple mutation sites in a single tube. This reduces reagent usage and significantly lowers detection costs. It is suitable for both single-sample detection and large-scale sample screening, while offering high sensitivity, high specificity, and high throughput.