68 results about "Membrane lipids" patented technology

The invention provides a composition for delivering at least one biologically active compound to a living organism, said composition comprising at least one micelle-forming membrane lipid

The invention discloses a new application of sodium hydrosulfide for promoting germination of wheat seeds under the stress of heavy metals, namely, the application is that the sodium hydrosulfide is taken as a sprout-promoting agent of germination in the soil polluted by heavy metals of Al<3+>, Cd<2+>, Cr<3+>, Cr<6+>, Pb<2+>, Hg<2> and the like. The invention can effectively improve the germinability of the wheat seeds under the stress of heavy metals, and induces the seeds to germinate fast and regularly. Research proves that the sodium hydrosulfide of the hydrogen sulfide donor is adopted to preprocess the wheat seeds, which can remarkably induce the activity expression of hydrolytic enzyme of amylase, protease, lipase and the like, in the seeds, enhances the oxygenolysis of storage matter, relieves the oxidative damage caused by the stress of the heavy metals in the seeds and maintains the lower membrane lipid overoxidation level in the seeds and the seedlings, thus being beneficial to the normal growth of embryo and radicle. The invention solves the key problem of restoring the production of the wheat in the areas polluted by heavy metals, has simple operation; the needed reagent is single and is easy to be obtained.

The invention relates to a new application of sodium hydrosulfide for promoting the morphogenesis of plant roots, namely, the application is that the sodium hydrosulfide is taken as root stimulate to promote the morphogenesis of side roots and adventitious roots of the seedling of the plant and the morphogenesis of the adventitious roots of cutting branches of the plant. The sodium hydrosulfide has obvious root-promoting effect to the seedlings of the plant such as rapes, mung beans, soybeans, cucumbers and the like, the hypocotyledonary axis of the cut segment thereof and the cutting branches of the plant such as willow branches, peach branches, grapes, batatas and the like, promotes the formation and growth of the side roots and the adventitious roots of the plant, enhances the water absorbing capacity of the plant, maintains the water balance in the body of the plant, reduces membrane lipid overoxidation damage of the hypocotyledonary axis of the cut segment and the cutting branches, maintains the relative stability of membrane structure of the explant and promotes the growth and propagation of the plant. The root-promoting agent can be used for growing roots of various plant seedlings, the hypocotyledonary axis of the cut segment and the cutting branches and is applicable to the root-promoting effect of promoting and culturing the seedlings of transplanting and cutting and the like, in the agriculture, forestry and gardening.

The invention relates to a plant freezing injury resistant nutrient solution containing potassium hydroxide hydrolysate of plant seed dregs and peat, phosphoric acid, a microelement fertilizer and polyatomic alcohol and a preparation method thereof. The nutrient solution has the functions of promoting photosynthesis of plants, reducing the moisture in plant cells, preventing membrane lipid from being converted from liquid to gel at low temperature, has the advantages of reasonable formulation, low energy consumption in production, simple and convenient production technique, convenient use, increase of the crop output, reduction of the labor intensity, and the like, and has wide application in the fields of agriculture, forestry and the like.

The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.

The invention provides infant formula milk powder rich in milk fat globule membrane protein and phospholipid. By adding alpha-lactalbumin, milk fat globule membrane and a physiological activator, suchas bifidobacterium animalis Bb-12, milk fat globule membrane lipids and protein, N-acetylneuraminic acid (sialic acid), SN-2 structure lipid, human milk oligosaccharides (HMOs) and human milk oligose(HMO). By simultaneously adjusting the using amount ratio of all the components, especially sphingomyelin, gangliosides, the milk fat globule membrane protein, the human milk oligosaccharides, the human milk oligose, adiponectin and lysozyme, and the proportion between mucin, xanthine oxidoreductase, mucin 15, CD 36, butyrophilin, adipose differentiation-related protein and fatty acid binding proteins, the content of the active ingredients in the milk powder is closer to the content of active ingredients in human milk, the constitution of the infant formula milk powder is optimized, and the formula milk powder obtained by using the method is closer to the human milk.

A composition in the form of a dry powder and which comprises: a) at least one membrane lipid, and b) at least one biologically active compound comprising a xanthine and/or a carboxylic acid, and which forms structured lipid assemblies when dispersed/dissolved in an aqueous medium. A method of preparing such a composition is also provided, together with a dispersion of structured lipid assemblies suspended in a solution of at least one biologically active compound and a method of preparing the same. The compositions and dispersions are suitable for use in creams and lotions for skin care.

The invention discloses a compound regulator for preventing premature senility and increasing yield of corn. Every kilogram of the compound regulator comprises 100-300 mg/kg of butane diacid, 1,000-3,000 mg/kg of chitin, 0.00001-0.00003 mg/kg of forchlorfenuron and 100,000-150,000 mg/kg of potassium dihydrogen phosphate (molecular formula: KH2PO4). The invention further discloses a method for applying the compound regulator. Due to the adoption of the compound regulator, the germination of corn seeds and the growth of seedlings can be promoted, the germination process of seeds is accelerated, the growth and the development of corn leaves and root systems are promoted, the leaf area and the root quantity of corn are increased, the chlorophyll content of leaves and the root system activity of corn are increased particularly at the middle and later periods of grain filling, the activities of anti-oxidases such as SOD (Superoxide Dismutase) and CAT (Catalase) and the like in the corn leaves and root systems are increased remarkably, the accumulation of active oxygen in the leaves and root systems is reduced, the peroxidation action of the membrane lipid is restrained, premature senility is prevented, and the yield of corn kernels is increased.

The invention provides a preparation method of guanidinated chitosan. By guanidination modification for chitosan, the biological alkalinity and the water solubility of the chitosan are improved, and the action capacity of the chitosan to an electronegative cell membrane is enhanced; the provided preparation method of the guanidinated chitosan aims to increase the grafting rate of chitosan guanidination and avoid side reaction. The invention further provides a method for preparing antibacterial nano microspheres from the guanidinated chitosan. The method comprises the steps of by taking dextran sulfate as a crosslinking agent, preparing the lysozyme-coated guanidinated chitosan nano microspheres; by the use of the puncture action of the guanidinated chitosan to the cell membrane, the stability of the outer membrane lipid structure of gram negative bacteria (G-) is destroyed to expose peptidoglycan on the inner wall layer, and the peptidoglycan is hydrolyzed through lysozyme; under the synergistic action of the guanidinated chitosan and the lysozyme-coated guanidinated chitosan nano microspheres, the inhibition effect on G- is enhanced.

The invention concerns antimicrobial compositions comprising free fatty acids emulsified with membrane lipids or hydrolysed derivatives thereof, and pharmaceutical formulations comprising same. The compositions can be used in the treatment of prophylaxis of microbial infections. They can also regulate the rate of blood clotting rendering them suitable for incorporation in catheter locking solutions and for use in wound care.

The invention discloses a DSE (Dark Septate Endophyte) bacterium and application thereof in promoting growth and preventing drought of blueberry, and belongs to the field of agricultural microorganisms. The invention provides the DSE bacterium R10, and the preservation number is CGMCC No.13882. When the DSE bacterium R10 is inoculated, the biomass of ground parts of blueberry, the photosynthetic rate of leaves, the protection enzyme activities such as SOD (Superoxide Dismutase) and POD (Peroxidase) are remarkably improved, and the contents of malondialdehyde and H2O2 in leaves are remarkably reduced, so that by adopting the strain, the accumulation of organic matters can be improved by increasing the photosynthetic rate of leave of host plants on one hand, on the other hand, reactive oxygen can be effectively eliminated by improving the activity of the protection enzymes, the peroxidation degree of drought degrees to membrane lipid is reduced, and the drought resistance of the blueberry is improved.

The invention belongs to the technical field of agricultural production, and particularly relates to a cold-resistant agent for brassica rapa L. The cold-resistant agent for the brassica rapa L. seedlings is mainly characterized by comprising raw materials as follows: 15-25 mg/L of ABA (abscisic acid) and the balance of water, wherein the purity of the ABA (abscisic acid) is higher than or equal to 99.9%. The cold-resistant agent aims to solve the problem that the brassica rapa L. in the northern cold and arid regions is vulnerable to low-temperature freezing damage, growth of the overground part and the root system of the brassica rapa L. is promoted with chemical control means, especially the thickening growth of roots, the outstanding performance refers to increase of dry matter accumulation, and safe wintering of the brassica rapa L. is guaranteed; meanwhile, permeability (relative electrical conductivity) of leaf cell membranes as well as accumulation of membrane lipid peroxidation malonaldehyde can be reduced, so that the low-temperature freezing degree of leaves of the brassica rapa L. is reduced, the cold resistance of the brassica rapa L. is remarkably improved, and the cold-resistant agent has great practical significance on brassica rapa L. production.

The invention provides a brain tissue membrane protein extraction method, and relates to the technical field of biology. A used scale removal agent is very mild; in addition, the concentration is verylow; a reducing agent and a protein allosteric agent are not used; the protein structure and activity can be maintained. Membrane lipid surrounding the extracted protein is not completely lost; a tertiary structure of the protein can be effectively remained, so that the combination state of the protein with other protein can be stabilized. By using the extraction method provided by the invention,80 percent of membrane protein or more can be effectively dissolved. The formula of a used buffer solution system is simple; only conventional reagents and instrument equipment in a laboratory are needed; the result and the activity of the protein are not influenced at all by the used stain removal agent and the dosage; the extracted membrane protein can be used for biochemical tests with higherdifficulty in co-immunoprecipitation and the like and higher protein extraction requirements.

The invention provides a method for improving survival rate of plant seeds by N-acylethanolamines (NAE) after ultralow-temperature preservation and application of N-acylethanolamines. The method comprises the following steps: taking plumular axes of the plant seeds and applying the N-acylethanolamines to the plumular axes of the plant seeds; carrying out pretreatment and an ultralow-temperature preservation experiment; and carrying out a germination force experiment to detect the vitality. Meanwhile, the invention provides application of the N-acylethanolamines to the ultralow-temperature preservation of the plant seeds. Before the seeds with the plumular axes which are sensitive to dehydration are subjected to the ultralow-temperature preservation, the survival rate of the seeds can be remarkably improved if an inhibitor NAE (100 micromoles) of PLDalpha is exogenously applied;lipidomics analysis is carried out in a whole process and shows that in an ultralow-temperature preservation process of the plumular axis seeds treated with the NAE, substrate phospholipids (PG, PE and PC) for hydrolysis of PLD alpha are remarkably improved and the formation of membrane lipid is changed, so that the survival rate of the plumular axes after the ultralow-temperature preservation process is improved; and the method has a very good application prospect in the preservation of germplasm resources of the sensitive seeds.

The present invention relates to a vaccine comprising a particle, said particle comprising (i) at least one Epstein-Barr virus (EBV) structural polypeptide, (ii) at least one EBV lytic polypeptide, (iii) membrane lipids, said particle being devoid of EBV DNA, wherein (a) the B-cell transformation capacity of one or more EBV polypeptides required for B-cell transformation as comprised in said particle is disabled while their immunogenicity is maintained; and/or (b) said particle is devoid of one or more EBV polypeptides required for B-cell transformation. Furthermore, the invention relates to a method for generating a particle, to a cell obtained in the method of the invention, a kit comprising the vaccine or the particle generated according to the method of the invention. Also, the invention relates to the use of the vaccine or the particle generated according to the method of the invention for generating CD8+ cells specific for an EBV antigen.

The invention relates to a rice cold-resistant agent and a preparation method thereof, and belongs to the technical field of agriculture. According to the rice cold-resistant agent, plant endogenous cold-resistance substances in carex lanceolata boott and pyrola rotundifolia are extracted, and are compounded with allogenic materials to promote rice growth, improve rice disease resistance and stress and allelopathy resistance, improve tillering, height, accumulation of above-ground and underground biomass and improve cold resistance of rice; and the cytomembrane stability can be kept due to mineral ions in mineral liquid, normal functions of cytomembrane can be maintained, related enzyme systems, such as catalase, peroxidase and superoxide dismutase, are improved to reduce the oxidation effect of free radicals for membrane lipid, the cytomembrane functions can be kept, the cell ultrastructure is prevented from being destroyed, cold resistance of plants can be improved, plant cells are stimulated to secrete peroxidase into cell walls and improve the activity of cells, the firmness of cell walls can e reinforced, and cell collapse injury caused by extracellular freezing and dehydration can be prevented.

The invention discloses application of red yeast rice to reduction of methane discharged by ruminant such as sheep. When the red yeast rice is added into ruminant feed to reduce methane discharged by ruminant, the additive amount of the red yeast rice in the daily ration of sheep depends on the average daily feed intake of the sheep, the content ratio of nutrients to lovastatin in the red yeast rice and the ratio of opening loops to closed loops of lovastatin. The red yeast rice is adopted for reducing methane discharged by sheep, and the added red yeast rice naturally containing lovastatin can competitively and powerfully inhibit 3-hydroxy-3-methylglutaryl-coenzyme A reductase and cut off the passage for synthesizing methane bacteria membrane lipid, thereby effectively inhibiting the growth of methane bacteria and eventually reducing methane discharged by ruminant such as sheep.

The invention discloses a method for cultivating pomegranates. The method includes steps of (1), managing fertilizers and water; (2), managing moisture, carrying out pruning, controlling growth of twigs and promoting blossom, thinning flowers and fruits, controlling diseases and insect pests, carrying out micro-membrane bagging by the aid of novel high-lipid membranes and carrying out uniform spraying on fruit surfaces. The method has the advantages that the fruit setting percentage of the pomegranates cultivated by the aid of the method can be increased, and the pomegranates rarely suffer from the diseases and insect pests, have uniform fruit sizes and are high in yield.

The present invention relates to novel bifunctional agents possessing antioxidant and antiarrhytmic activity, methods for the synthesis of the same and their applications in treating ischemia-reperfusion injury, as well as a variety of disorders related to free radicals and/or arrhythmias. These bifunctional drugs should preferentially segregate in the membrane and produce their antiarrhytmic effects while, at the same time, help in protecting the membrane lipids by scavenging free radicals. The present invention comprises compounds represented by Formula (I), wherein R₁, R₂, R₃, R₄, R₅ are further defined

The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.

The invention relates to a hydroxydecyl quinone derivative for treating or preventing nerve diseases. Specifically, the invention relates to the hydroxydecyl quinone derivative as shown in formula I, a pharmaceutical composition containing the compound as shown in the formula I, and a preparation method and uses of the hydroxydecyl quinone derivative in preparation of medicaments for cerebrovascular diseases. The compound disclosed by the invention can effectively treat or prevent the diseases or disease symptoms: the cerebrovascular diseases, improve cerebral metabolism and improve mental symptoms, activate brain mitochondrial respiratory activity, improve cerebral energy metabolism of cerebral ischemia, improve the utilization rate of cerebral glucose, increase the production of cerebral triphosadenine, inhibit the generation of lipid hydroperoxide from brain mitochondria and inhibit the membrane disorder caused by overoxidation action of the brain mitochondrial membrane lipid, reduce impaired brain functions caused by chronic cerebrovascular diseases, cerebral trauma and the like, improve subjective symptoms, language, anxiety, depression, hypomnesia, intelligence decrease and other mental and behavioral disorders, promote the intelligence, and activate brain mitochondrial functions, improve the cerebral energy metabolism and improve brain functions.