Method for improving survival rate of plant seeds by N-acylethanolamines after ultralow-temperature preservation and application of N-acylethanolamines

A technology of acylethanolamine and plant seeds, which is applied in the fields of plant preservation, botanical equipment and methods, and application, can solve unseen problems, and achieve the effect of improving survival rate and good application prospect.

Inactive Publication Date: 2015-02-25
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no report in the prior art that exogenous application

Method used

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  • Method for improving survival rate of plant seeds by N-acylethanolamines after ultralow-temperature preservation and application of N-acylethanolamines
  • Method for improving survival rate of plant seeds by N-acylethanolamines after ultralow-temperature preservation and application of N-acylethanolamines

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Experimental program
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Effect test

Embodiment 1

[0025] Exogenous application of 100 μM N-acylethanolamines (N-acylethanolamines, NAE) significantly increased the survival rate and regeneration rate of hypocotyls of Yunnan camellia and Pu'er tea during cryopreservation.

[0026] In this experiment, the hypocotyls of the intermediate seeds of Yunnan camellia (Camillia reticulate) and tea (Camillia sinensis) were taken for pretreatment and cryopreservation experiments, and germination tests were carried out to detect vigor. After peeling the seeds of Yunnan camellia and Puer tea, soak them in 75% alcohol for 1 minute for surface disinfection, and then wash them with clean water. Under aseptic conditions, the hypocotyls of the seeds were taken out, and every 10 hypocotyls was regarded as a repetition, and each treatment had 5 repetitions, and they were placed in the treatment solution of 1ml water, 100μM DMSO and 100μM NAE respectively, and placed in the dark at 25°C. Process for 3 hours. The hypocotyls were taken out and drie...

Embodiment 2

[0028] Exogenous application of 100 μM N-acylethanolamines (N-acylethanolamines, NAE) changed the membrane lipid composition of Yunnan Camellia hypocotyls during cryopreservation, thereby improving the survival rate and regeneration rate of hypocotyls after cryopreservation.

[0029] In this experiment, the hypocotyls of the intermediate seed Yunnan camellia (Camillia reticulate) seeds were selected for pretreatment and cryopreservation experiments, and lipid detection and analysis were carried out. After peeling the seeds of Camellia yunnanensis, soak them in 75% alcohol for 1 minute for surface disinfection, and then wash them with clean water. Under aseptic conditions, the hypocotyls of the seeds were taken out, and every 10 hypocotyls was regarded as a repetition, and each treatment had 5 repetitions, and they were placed in the treatment solution of 1ml water, 100μM DMSO and 100μM NAE respectively, and placed in the dark at 25°C. Process for 3 hours. The hypocotyls were ...

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Abstract

The invention provides a method for improving survival rate of plant seeds by N-acylethanolamines (NAE) after ultralow-temperature preservation and application of N-acylethanolamines. The method comprises the following steps: taking plumular axes of the plant seeds and applying the N-acylethanolamines to the plumular axes of the plant seeds; carrying out pretreatment and an ultralow-temperature preservation experiment; and carrying out a germination force experiment to detect the vitality. Meanwhile, the invention provides application of the N-acylethanolamines to the ultralow-temperature preservation of the plant seeds. Before the seeds with the plumular axes which are sensitive to dehydration are subjected to the ultralow-temperature preservation, the survival rate of the seeds can be remarkably improved if an inhibitor NAE (100 micromoles) of PLDalpha is exogenously applied;lipidomics analysis is carried out in a whole process and shows that in an ultralow-temperature preservation process of the plumular axis seeds treated with the NAE, substrate phospholipids (PG, PE and PC) for hydrolysis of PLD alpha are remarkably improved and the formation of membrane lipid is changed, so that the survival rate of the plumular axes after the ultralow-temperature preservation process is improved; and the method has a very good application prospect in the preservation of germplasm resources of the sensitive seeds.

Description

Technical field: [0001] The invention belongs to the technical field of seed physiology and seed storage, and in particular relates to a method for improving the survival rate of plant seeds after cryopreservation by using N-acylethanolamine and its application. Background technique: [0002] Seed preservation is an important means of long-term preservation of plant germplasm resources. It can provide important physical materials and genetic resources for the fields of new crop variety selection and variety improvement, ecosystem restoration or reconstruction, and plant diversity protection. According to the latest statistical report of the United Nations World Food and Agriculture Organization (FAO), from 1996 to 2009, the germplasm resources of wild plants collected and preserved in various seed banks around the world increased from 900,000 to 1.332 million (FAO, 2010). However, 7-25% of plants (up to 47% in tropical rainforests) produce seeds that cannot be preserved by c...

Claims

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Application Information

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IPC IPC(8): A01N3/00A01C1/00G01N5/04
Inventor 李唯奇陈虹颖马俊超
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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