87 results about "Cell Membrane Lipids" patented technology

The invention provides a composition for delivering at least one biologically active compound to a living organism, said composition comprising at least one micelle-forming membrane lipid

The present application describes compositions, methods and devices for detecting anti-lipid antibodies and diagnosing diseases such as syphilis. In particular, methods for immobilizing lipid antigens (including cardiolipin, lecithin, and cholesterol) on solid supports (eg, nitrocellulose membranes) are described. The ability to immobilize lipid antigens on membranes fulfills a long known need for a membrane-based assay for the detection of anti-lipid antibodies. An immunoassay device for simultaneous treponema pallidum and non-treponemal testing is also described.

The invention discloses a new application of sodium hydrosulfide for promoting germination of wheat seeds under the stress of heavy metals, namely, the application is that the sodium hydrosulfide is taken as a sprout-promoting agent of germination in the soil polluted by heavy metals of Al<3+>, Cd<2+>, Cr<3+>, Cr<6+>, Pb<2+>, Hg<2> and the like. The invention can effectively improve the germinability of the wheat seeds under the stress of heavy metals, and induces the seeds to germinate fast and regularly. Research proves that the sodium hydrosulfide of the hydrogen sulfide donor is adopted to preprocess the wheat seeds, which can remarkably induce the activity expression of hydrolytic enzyme of amylase, protease, lipase and the like, in the seeds, enhances the oxygenolysis of storage matter, relieves the oxidative damage caused by the stress of the heavy metals in the seeds and maintains the lower membrane lipid overoxidation level in the seeds and the seedlings, thus being beneficial to the normal growth of embryo and radicle. The invention solves the key problem of restoring the production of the wheat in the areas polluted by heavy metals, has simple operation; the needed reagent is single and is easy to be obtained.

An irinotecan preparation having irinotecan and / or a salt thereof highly carried by a closed vesicle carrier, which exhibits a strikingly prolonged retention in blood as compared with that of conventional irinotecan liposome preparations and is capable of being present in blood for a prolonged period of time. There is provided an irinotecan preparation comprising closed vesicles formed of a lipid membrane wherein irinotecan and / or a salt thereof is sealed in a concentration of at least 0.07 mol / mol (medicine mol / total membrane lipid mol). The irinotecan preparation preferably has an ion gradient between an inner water phase and an outer water phase within the closed vesicles. The closed vesicles are preferably liposomes, and the liposomes preferably have only the external surface thereof modified with a surface modifier containing a hydrophilic polymer.

The invention relates to a new application of sodium hydrosulfide for promoting the morphogenesis of plant roots, namely, the application is that the sodium hydrosulfide is taken as root stimulate to promote the morphogenesis of side roots and adventitious roots of the seedling of the plant and the morphogenesis of the adventitious roots of cutting branches of the plant. The sodium hydrosulfide has obvious root-promoting effect to the seedlings of the plant such as rapes, mung beans, soybeans, cucumbers and the like, the hypocotyledonary axis of the cut segment thereof and the cutting branches of the plant such as willow branches, peach branches, grapes, batatas and the like, promotes the formation and growth of the side roots and the adventitious roots of the plant, enhances the water absorbing capacity of the plant, maintains the water balance in the body of the plant, reduces membrane lipid overoxidation damage of the hypocotyledonary axis of the cut segment and the cutting branches, maintains the relative stability of membrane structure of the explant and promotes the growth and propagation of the plant. The root-promoting agent can be used for growing roots of various plant seedlings, the hypocotyledonary axis of the cut segment and the cutting branches and is applicable to the root-promoting effect of promoting and culturing the seedlings of transplanting and cutting and the like, in the agriculture, forestry and gardening.

The invention relates to a multi-functional peanut growth inhibitor which is composed by the weight ratio of following components, paclobutrazol to prohexadione calcium to uniconazole is 3: 1.2-1.5: 0.8-1.2. The invention not only can effectively control the growth of the vegetative part of the peanut on the ground, can prevent the peanut from overgrowing and lodging, but also can effectively avoid the side effect brought by general growth inhibitor (e.g. paclobutrazol). At the same time, in the growth anaphase of the peanut, the invention can remarkably improve LAI (leaf area index), effective LAI, leaves membrane-lipid protective enzymatic activity (SOD, POD, CAT), Pr (soluble protein) content and so on, can reduce MDA content and can postpone the senescence rate of peanut leaves. More importantly, the invention can remarkably improve the output of pods. The production can be increased by 16.6 percent- 23.0 percent more than the production without spraying any inhibitor, and can be increased by 7.6 percent- 13.7 percent more than the production only spraying paclobutrazol. The invention solves the problem that the overgrowth and presenility can not be prevented synchronously in the production process of the peanuts.

The invention relates to a plant freezing injury resistant nutrient solution containing potassium hydroxide hydrolysate of plant seed dregs and peat, phosphoric acid, a microelement fertilizer and polyatomic alcohol and a preparation method thereof. The nutrient solution has the functions of promoting photosynthesis of plants, reducing the moisture in plant cells, preventing membrane lipid from being converted from liquid to gel at low temperature, has the advantages of reasonable formulation, low energy consumption in production, simple and convenient production technique, convenient use, increase of the crop output, reduction of the labor intensity, and the like, and has wide application in the fields of agriculture, forestry and the like.

The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.

The invention provides infant formula milk powder rich in milk fat globule membrane protein and phospholipid. By adding alpha-lactalbumin, milk fat globule membrane and a physiological activator, suchas bifidobacterium animalis Bb-12, milk fat globule membrane lipids and protein, N-acetylneuraminic acid (sialic acid), SN-2 structure lipid, human milk oligosaccharides (HMOs) and human milk oligose(HMO). By simultaneously adjusting the using amount ratio of all the components, especially sphingomyelin, gangliosides, the milk fat globule membrane protein, the human milk oligosaccharides, the human milk oligose, adiponectin and lysozyme, and the proportion between mucin, xanthine oxidoreductase, mucin 15, CD 36, butyrophilin, adipose differentiation-related protein and fatty acid binding proteins, the content of the active ingredients in the milk powder is closer to the content of active ingredients in human milk, the constitution of the infant formula milk powder is optimized, and the formula milk powder obtained by using the method is closer to the human milk.

The present invention relates to hydrophobically modified proteins and methods for their preparation. Hydrophobic groups are attached to the surface amino acid residues of proteins. Hydrophobic groups can be lipids or peptides. Alternatively, the protein can be derivatized by various reactive reactions that suspend hydrophobic structures on the protein. Preferred proteins are mammalian and selected from the group consisting of Sonic, Indian and Desert hedgehog. Hydrophobic groups can be used as convenient linking groups to attach vesicles such as cell membranes, liposomes or micelles.

Use of polyunsaturated fatty acid derivatives as medicaments or functional foods. The present invention relates to the use of 1,2-fatty acid derivatives in the treatment or prevention of common diseases whose etiology is based on alterations (of any type) of the cell membrane lipids, for example, changes in levels, in the composition or in the structure of these lipids. In addition, for diseases in which the regulation of lipid composition and of the structure of the membranes (or proteins that interact with membranes) causes the reversion of pathological state.

A composition in the form of a dry powder and which comprises: a) at least one membrane lipid, and b) at least one biologically active compound comprising a xanthine and / or a carboxylic acid, and which forms structured lipid assemblies when dispersed / dissolved in an aqueous medium. A method of preparing such a composition is also provided, together with a dispersion of structured lipid assemblies suspended in a solution of at least one biologically active compound and a method of preparing the same. The compositions and dispersions are suitable for use in creams and lotions for skin care.

Use of polyunsaturated fatty acid derivatives as medicaments or functional foods. The present invention relates to the use of 1,2-fatty acid derivatives in the treatment or prevention of common diseases whose etiology is based on alterations (of any type) of the cell membrane lipids, for example, changes in levels, in the composition or in the structure of these lipids. In addition, for diseases in which the regulation of lipid composition and of the structure of the membranes (or proteins that interact with membranes) causes the reversion of pathological state.

Compositions for increased antioxidant potency of natural vitamin E (d-alpha-tocopherol), comprising alpha-, beta-, delta-, and gamma-forms of tocopherols and tocotrienols are disclosed. All of these compositions provide 400 International Units (IU), based on one mg of d-alpha-tocopherol provides 1.49 IU. These compositions showed antioxidant activities superior to natural d-alpha-tocopherol. These compositions are designed to provide protection of the cell membrane lipid layer, and protection against heart disease, cancer, and eye disease.

An artificial lipid bilayer membrane formation device is disclosed, which includes: an upper solution chamber (first solution chamber) and a lower solution chamber (second solution chamber), both of which are filled with aqueous solution. It further includes a partition wall disposed between the upper solution chamber and the lower solution chamber so as to part the upper and lower solution chambers from each other. The partition wall has an opening, and a first lipid solution is applied to a portion around the opening, thereby forming an artificial lipid bilayer membrane on the opening. Further, in the formation device, a tubule for lipid substitution is attached to the partition wall so as to be positioned on a bulk phase of the artificial lipid bilayer membrane. A second lipid solution is added via the tubule, thereby forming an artificial lipid bilayer membrane whose lipid composition is changed.

The invention discloses a compound regulator for preventing premature senility and increasing yield of corn. Every kilogram of the compound regulator comprises 100-300 mg / kg of butane diacid, 1,000-3,000 mg / kg of chitin, 0.00001-0.00003 mg / kg of forchlorfenuron and 100,000-150,000 mg / kg of potassium dihydrogen phosphate (molecular formula: KH2PO4). The invention further discloses a method for applying the compound regulator. Due to the adoption of the compound regulator, the germination of corn seeds and the growth of seedlings can be promoted, the germination process of seeds is accelerated, the growth and the development of corn leaves and root systems are promoted, the leaf area and the root quantity of corn are increased, the chlorophyll content of leaves and the root system activity of corn are increased particularly at the middle and later periods of grain filling, the activities of anti-oxidases such as SOD (Superoxide Dismutase) and CAT (Catalase) and the like in the corn leaves and root systems are increased remarkably, the accumulation of active oxygen in the leaves and root systems is reduced, the peroxidation action of the membrane lipid is restrained, premature senility is prevented, and the yield of corn kernels is increased.

The invention provides a preparation method of guanidinated chitosan. By guanidination modification for chitosan, the biological alkalinity and the water solubility of the chitosan are improved, and the action capacity of the chitosan to an electronegative cell membrane is enhanced; the provided preparation method of the guanidinated chitosan aims to increase the grafting rate of chitosan guanidination and avoid side reaction. The invention further provides a method for preparing antibacterial nano microspheres from the guanidinated chitosan. The method comprises the steps of by taking dextran sulfate as a crosslinking agent, preparing the lysozyme-coated guanidinated chitosan nano microspheres; by the use of the puncture action of the guanidinated chitosan to the cell membrane, the stability of the outer membrane lipid structure of gram negative bacteria (G-) is destroyed to expose peptidoglycan on the inner wall layer, and the peptidoglycan is hydrolyzed through lysozyme; under the synergistic action of the guanidinated chitosan and the lysozyme-coated guanidinated chitosan nano microspheres, the inhibition effect on G- is enhanced.

The invention provides a traditional Chinese medicine suppository which is specially used for treating gynaecological vaginitis and a preparation method thereof, according to the pathogenic mechanism and therapeutic principle of the gynaecological vaginitis and the theory of modern traditional Chinese medicine. In the suppository, radices sophorae flavescentis, herba violae, fructus citri sarcodactyli, radix gentianaes and szechuan lovage rhizome, which can clear heat, dry dampness, dispel wind, arrest itching, antisepticise, subdue inflammation and disinfest, are taken as monarch drugs; fructus cnidii, safflower, motherwort herbs, honeysuckle and hydrargyri subchloridum, which can dispel wind, disinfest, treat pruritus vulvae and leucorrhea for cold wetness, are taken as ministerial drugs; fructus kochiae, catechu, matricaria indica and garden burnet root, which can strengthen the curative effects of the monarch drugs and the ministerial drugs, are taken as excipients; and mint, skullcap, lotus plumule, divaricate saposhnikovia root, borneol and seabuckthorn seed oil, which can clear away the heart fire, nourish yin, promote the production of body fluid, and eliminate wetness-evil with fragrant flavor, are taken as adjuvant drugs. The combination of the monarch drugs, ministerial drugs, excipients and the adjuvant drugs can display the inwardness of the drugs and combine the effects of the drugs, thereby changing the composition of cell membrane lipids and killing the epiphyte. Therefore, the suppository has good curative effects on the vulvitis, colpomycosis and trichomonas vaginitis with short course of treatment and high cure rate, and can realize the radical cure of the diseases.

The invention concerns antimicrobial compositions comprising free fatty acids emulsified with membrane lipids or hydrolysed derivatives thereof, and pharmaceutical formulations comprising same. The compositions can be used in the treatment of prophylaxis of microbial infections. They can also regulate the rate of blood clotting rendering them suitable for incorporation in catheter locking solutions and for use in wound care.

The invention concerns antimicrobial compositions comprising free fatty acids emulsified with membrane lipids or hydrolyzed derivatives thereof, and pharmaceutical formulations comprising same. The compositions can be used in the treatment of prophylaxis of microbial infections. They can also regulate the rate of blood clotting rendering them suitable for incorporation in catheter locking solutions and for use in wound care.

The invention discloses a DSE (Dark Septate Endophyte) bacterium and application thereof in promoting growth and preventing drought of blueberry, and belongs to the field of agricultural microorganisms. The invention provides the DSE bacterium R10, and the preservation number is CGMCC No.13882. When the DSE bacterium R10 is inoculated, the biomass of ground parts of blueberry, the photosynthetic rate of leaves, the protection enzyme activities such as SOD (Superoxide Dismutase) and POD (Peroxidase) are remarkably improved, and the contents of malondialdehyde and H2O2 in leaves are remarkably reduced, so that by adopting the strain, the accumulation of organic matters can be improved by increasing the photosynthetic rate of leave of host plants on one hand, on the other hand, reactive oxygen can be effectively eliminated by improving the activity of the protection enzymes, the peroxidation degree of drought degrees to membrane lipid is reduced, and the drought resistance of the blueberry is improved.

The invention provides a brain tissue membrane protein extraction method, and relates to the technical field of biology. A used scale removal agent is very mild; in addition, the concentration is verylow; a reducing agent and a protein allosteric agent are not used; the protein structure and activity can be maintained. Membrane lipid surrounding the extracted protein is not completely lost; a tertiary structure of the protein can be effectively remained, so that the combination state of the protein with other protein can be stabilized. By using the extraction method provided by the invention,80 percent of membrane protein or more can be effectively dissolved. The formula of a used buffer solution system is simple; only conventional reagents and instrument equipment in a laboratory are needed; the result and the activity of the protein are not influenced at all by the used stain removal agent and the dosage; the extracted membrane protein can be used for biochemical tests with higherdifficulty in co-immunoprecipitation and the like and higher protein extraction requirements.

The invention belongs to the field of otology repair materials, and relates to a biological patch for tympanic membrane repair and a preparation method thereof. The otology repair patch of the invention overcomes the shortcomings of the prior patch due to an improper decellularizing process and a complicated process, and causes serious loss of effective active ingredients in the ECM. The biological patch adopts the plant-derived saponin as a decellularizing reagent, which avoids the residue and toxicity of a chemical reagent, and the mild decellularizing reagent is highly targeted and mainly binds to the cell membrane lipid; the decellularizing reagent has little damage on an ECM stereoscopic three-dimensional structure and the active components thereof; and more biologically active components such as glycosaminoglycan (including hyaluronic acid) can be retained. The patch of the invention has a better ECM scaffold structure and more active components, and has stronger ability to induce cell chemotaxis and proliferation, is beneficial to faster and better repair of the tympanic membrane, and also has a certain anti-infective effect; at the same time, the method of the invention issimple and convenient to operate, and has no damage to the ECM.

The present invention relates to a vaccine comprising a particle, said particle comprising (i) at least one Epstein-Barr virus (EBV) structural polypeptide, (ii) at least one EBV lytic polypeptide, (iii) membrane lipids, said particle being devoid of EBV DNA, wherein (a) the B-cell transformation capacity of one or more EBV polypeptides required for B-cell transformation as comprised in said particle is disabled while their immunogenicity is maintained; and / or (b) said particle is devoid of one or more EBV polypeptides required for B-cell transformation. Furthermore, the invention relates to a method for generating a particle, to a cell obtained in the method of the invention, a kit comprising the vaccine or the particle generated according to the method of the invention. Also, the invention relates to the use of the vaccine or the particle generated according to the method of the invention for generating CD8+ cells specific for an EBV antigen.

The invention discloses application of red yeast rice to reduction of methane discharged by ruminant such as sheep. When the red yeast rice is added into ruminant feed to reduce methane discharged by ruminant, the additive amount of the red yeast rice in the daily ration of sheep depends on the average daily feed intake of the sheep, the content ratio of nutrients to lovastatin in the red yeast rice and the ratio of opening loops to closed loops of lovastatin. The red yeast rice is adopted for reducing methane discharged by sheep, and the added red yeast rice naturally containing lovastatin can competitively and powerfully inhibit 3-hydroxy-3-methylglutaryl-coenzyme A reductase and cut off the passage for synthesizing methane bacteria membrane lipid, thereby effectively inhibiting the growth of methane bacteria and eventually reducing methane discharged by ruminant such as sheep.

The invention provides a preparation method of a nanometer material loaded BiOBr / fabric. Specifically, the method comprises dissolving KBr in water, mixing in fabric to obtain a mixed solution A; dissolving Bi(NO3)3*5H2O into acetic acid aqueous solution, and adding the solution into the mixed solution A, stirring the mixture at room temperature, performing ultrasonic treatment, standing and drying to obtain the prepared nanometer material loaded BiOBr / fabric. The prepared nanometer material loaded BiOBr / fabric is applied to microcystis aeruginosa removal. Under light, the nanometer material loaded BiOBr / fabric can consume O2 in culture media and produce highly oxidizing free radicals on the surface of BiOBr particles to deep degrade and oxidize photosynthetic pigments, phycobiliprotein and on-membrane polyunsaturated fatty acids and further to cause collapse of the photosynthetic function, hindering of protein synthesis and peroxidation of cell membrane lipids, thereby damaging alga cells and inhibiting growths of alga cells.

The invention discloses a method for storage and preservation of Pleurotus eryngii. According to the method, a part of culture mediums is retained when Pleurotus eryngii is picked, and then the retained part of the culture mediums is sealed with a PE-LD fresh-keeping ziplock bag and stored at 3-5 DEG C. Through the method, effective maintenance of the hardness and toughness of Pleurotus eryngii, reduction of formation of lignin and maintenance of the unique crisp taste of Pleurotus eryngii are achieved, and reduction of the production amount of malondialdehyde (MDA) and superoxide anions (O<2><->) in the late stage of storage, promotion of activity increase of catalase (CAT), reduction of cell membrane lipid peroxidation, active oxygen accumulation and other abnormal metabolism caused by adversity stress and effective delaying of the aging process of Pleurotus eryngii during storage are achieved; and meanwhile reduction of the loss of soluble protein, reducing sugar and flavonoids andmaximum maintenance of nutrients of Pleurotus eryngii during storage are achieved. The method has the advantages of a significant effect of prolonging of the shelf life of Pleurotus eryngii, low cost,convenient operation and easy promotion, and provides a new method for storage, transportation and preservation of Pleurotus eryngii and other edible mushrooms and a new idea for fresh-keeping technologies.