59 results about "Cell Membrane Lipids" patented technology

The invention discloses a targeted delivery method of a plant-derived biological regulator, and relates to the technical field of bioengineering, and the targeted delivery method comprises the following steps: in the application process of an electroporation technology, monitoring and acquiring cell membrane lipid change data information in real time through a biosensor technology; the method comprises the following steps: preprocessing original data acquired by a biosensor, and organizing and storing the preprocessed data according to rules to form a structured data set; the system can accurately control the state of the plant cell membrane by monitoring the lipid change of the cell membrane in real time and combining intelligent evaluation and dynamic adjustment of the electric field parameters. When membrane lipid is oversaturated, the electric field intensity and the pulse frequency are dynamically adjusted, membrane damage is reduced, cell integrity is protected, and meanwhile effective delivery of allogenic materials is ensured. According to the method, the stability and the accuracy of an electroporation technology are improved, the targeted delivery of the plant-derived biological regulator is optimized, and the application potential of the plant-derived biological regulator in the fields of agriculture and biomedicine is expanded.

The invention relates to the technical field of genetic engineering, and discloses an engineering bacterium for high-yield production of D-pantothenic acid based on sugar uptake regulation and a preparation method of the engineering bacterium. The method comprises the following steps: firstly, stabilizing an RNAP promoter open complex formed on an rRNA (Ribonucleic Acid) gene by intensively expressing a necessary global regulation transcription factor CarD of mycobacterium neoaurum; secondly, performing enhanced expression on mycobacterium neoaurum Rpf / Sps family protein RpfB which serves as a resuscitation promoting factor to play a role and catalyze peptidoglycan synthesis to accelerate cell wall growth; meanwhile, the inactivated cell wall lipoprotein Lpp accumulates the outer membrane vesicles; in addition, lipoprotein NlpI responsible for adjusting the activity of endopeptidase MopS and PBP4 is deleted, and the super vesicles are generated; in addition, the mutant membrane lipid asymmetric pathway protein MlaE disturbs the homeostasis of the outer membrane and increases the formation of vesicles; in addition, the membrane anchoring protein TolA is destroyed to improve the membrane permeability; and finally, the escherichia coli gene engineering strain with greatly improved D-pantothenic acid production performance is obtained.

The invention discloses a functional lipid compound for nano-liposome wrapping and a preparation method, and relates to the technical field of functional lipid compounds, the functional lipid compound comprises (a) a polyphenol-lipid covalent conjugate which is composed of a polyphenol active unit selected from curcumin, dihydroquercetin or derivatives thereof, and (b) a lipid covalent conjugate which is composed of a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof and a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof; the conjugate is covalently linked to a phosphatidyl ethanolamine or cholesterol skeleton through a cleavable linking group, and the conjugate can be integrated into a nano-liposome membrane and has the functions of structural support and biological activity; (b) a microenvironment-responsive functional phospholipid, which is a phospholipid derivative having a functional group responsive to an inflammatory microenvironment specific stimulus modified on a hydrophilic head group or a hydrophobic chain of a phospholipid molecule, the specific stimulus being selected from local pH < = 6.5, reactive oxygen species (ROS) concentration > = 10 [mu] M or matrix metalloproteinase MMP-2 / 9 overexpression; and (c) a bionic exosome membrane lipid component, wherein the bionic exosome membrane lipid component comprises sphingosine-based glycolipid or sulfated cholesterol separated from the plant-derived exosome.

Nanolipoprotein particles having at least a scaffold protein component and a membrane lipid component and related compositions, methods and systems are described. The membrane lipid component includes at least one or more membrane forming lipids, one or more polymerized lipids and / or one or more polymerizable lipids.

The invention discloses a preparation method of a high-activity mitochondrial targeting MAPS polypeptide preparation, and relates to the technical field of biologication.The preparation method comprises the steps of S1, polypeptide design and synthesis, S2, thiol oriented cross-linking, S3, cutting and purification, S4, liposome assembly and S5, freeze-drying preparation molding.The preparation method has the advantages that by designing an amphipathic alpha-spiral polypeptide structure, the high-activity mitochondrial targeting MAPS polypeptide preparation is obtained; a stable helical conformation is spontaneously formed in a physiological environment by utilizing the alternative arrangement characteristic of hydrophobic and hydrophilic residues; the hydrophobic surface of the conformation can effectively interact with the mitochondrial membrane lipid bilayer, so that the transmembrane resistance is reduced; meanwhile, the hydrophilic surface is combined with the high negative membrane potential of the mitochondrial inner membrane through charge interaction to form a directional driving effect, so that the active transfer efficiency of the polypeptide under the mitochondrial membrane potential is remarkably improved; in addition, the integrated mitochondrial positioning signal further specifically recognizes the mitochondrial outer membrane transporter, the polypeptide is assisted to be accurately anchored to the targeting site, and non-specific diffusion is reduced.

The invention belongs to the technical field of biological medicines, and particularly relates to a thiophenol compound as well as a preparation method and application thereof. The prepared thiophenol compound is applied to human kidney cells HK-2 and living mice, it is found that the compound can remarkably inhibit peroxidation of cell membrane lipid molecules induced by hypoxia-reoxygenation and further induce cell ferroptosis, and the thiophenol compound has a long-term stable inhibition effect. By pretreating the compound, the levels of creatinine and urea nitrogen in serum after kidney injury can be remarkably reduced, which indicates that the compound can relieve acute kidney injury. Besides, the compound prepared by the invention is used for pretreatment, so that kidney external marrow region injury caused by ischemia-reperfusion is remarkably relieved, lesions such as renal tubular epithelial cell necrosis and the like are effectively relieved, and a new treatment strategy is provided for ferroptosis inhibitors when the compound is applied to kidney ischemia-reperfusion injury repair.

The present invention belongs to the field of biomedicine technology, and in particular to a thiophenol compound and its preparation method and application. The present invention uses the prepared thiophenol compound for human kidney cells HK-2 and live mice, and finds that the above-mentioned compound can significantly inhibit the peroxidation of cell membrane lipid molecules induced by hypoxia-reoxygenation, and then induces cell ferroptosis, and has a long-term stable inhibitory effect. The levels of creatinine and urea nitrogen in serum after kidney damage can be significantly reduced by pre-treating the compound, indicating that it can alleviate acute kidney injury. In addition, the compound pre-treatment prepared by the present invention significantly reduces the damage to the outer medullary region of the kidney caused by ischemia-reperfusion, and effectively alleviates lesions such as renal tubular epithelial cell necrosis, and its application in the repair of renal ischemia-reperfusion injury provides a new treatment strategy for ferroptosis inhibitors.

The invention relates to a genetically engineered bacterium with high capric acid tolerance as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as a starting strain, an aaS gene is knocked out, and meanwhile, a mla Z gene and a mla Y gene are overexpressed; wherein the Gene ID (Identity) of the nucleotide sequence of the aaS gene is 947315; the Gene ID (Identity) of the nucleotide sequence of the mla Z gene is 882402; and the Gene ID (Identity) of the nucleotide sequence of the mla Y gene is 882401. The aaS gene in the escherichia coli is knocked out, and the mla Z and mla Y genes are overexpressed at the same time, so that the membrane lipid strength of the escherichia coli engineering bacteria is effectively enhanced, and the genetically engineered bacteria show better robustness and higher tolerance in the fermentation process of a high-concentration substrate capric acid. When the capric acid is fed to the maximum concentration of 3.0 g / L, the yield of the trans-2-decenoic acid can reach 1.47 g / L, the conversion rate is 49.13%, and the yield is remarkably improved.

The invention belongs to the technical field of biology, and particularly relates to an oil red O modified coupling coloring agent and a frozen section dyeing method thereof. The oil red O modified coupling coloring agent comprises a carboxylated oil red O stock solution, a coupling agent, a buffer solution and Triton X-100, the carboxylated oil red O stock solution is modified through succinic anhydride, EDC and NHS, oil red O can be converted into a specific molecular dye from a hydrophobicity-dependent similar-dissolvable non-specific physical dye, lipid molecules are actively combined, and the specific molecular dye is converted into the specific molecular dye. And different types of lipid molecules are dyed, other cell structures are not adhered, and a non-specific background is eliminated, so that weak signals such as tiny lipid droplets, cell membrane lipid and the like can be clearly dyed and captured, and the imaging quality and the accuracy of quantitative analysis are improved.

The present disclosure relates to the field of biotechnology, and in particular to method for modifying a high-yield L-lysine-producing strain engineering and application thereof. The present disclosure provides a recombinant strain obtained by knocking out the cbrB gene and / or the cbrC gene in the genome of E. coli. Fermentation of the recombinant strain can increase the yield and conversion rate of L-lysine and reduce the mortality rate of the strain during the fermentation process. The present disclosure has significant application value. Through experimental studies, it was found that these two genes have a synergistic effect on E. coli. L-lysine hydrochloride imposes osmotic stress resistance on the strains, and silencing the related gene proteins can alter the membrane lipid composition (increasing a proportion of unsaturated fatty acids), maintain membrane fluidity, improve tolerance to lysine hydrochloride, and reduce the mortality rate in the late stage of fermentation.

The invention relates to the technical field of biological medicine, and particularly discloses a macrophage membrane coated plateau encephaledema drug carrier and a preparation method and application thereof, and the macrophage membrane coated plateau encephaledema drug carrier is composed of a nanoparticle core and a macrophage membrane shell layer wrapping the core; the nanoparticle core comprises an ROS-responsive carrier material and carbon monoxide release molecules MnCO entrapped in the ROS-responsive carrier material; the content of phosphatidylserine in the shell layer of the macrophage membrane is not less than 5 mol% of the total phospholipid of the membrane. According to the invention, a bionic delivery system of ROS response type MnCO nanoparticles coated with a macrophage membrane is constructed, phosphatidylserine on the surface of the membrane is utilized to actively activate a microglial cell Trem2 receptor, a positive feedback cycle of targeting phagocytosis-CO release-Trem2 up-regulation is formed, and at the same time, fatty acid oxidative metabolism reprogramming is promoted by means of degraded membrane lipid, so that the biomimetic delivery system is used for preparing the ROS response type MnCO nanoparticles. And multi-mechanism synergistic efficient brain-targeted therapy is realized.

Nanolipoprotein particles having at least a scaffold protein component and a membrane lipid component and related compositions, methods and systems are described. The membrane lipid component includes at least one or more membrane forming lipids, one or more polymerized lipids and / or one or more polymerizable lipids.

This invention discloses a method for isolating and preparing Chlorella exovesicles. Specifically, Chlorella, conventionally cultured to the early logarithmic growth phase, is induced to develop in a nitrogen-limited medium. The culture medium is then concentrated using a tangential flow filtration system. Finally, the concentrate is subjected to density gradient centrifugation with iodixanol. After centrifugation, components within the density range of 1.10–1.12 g / mL are collected to obtain the Chlorella exovesicle product. This invention achieves concentrated and precise collection of Chlorella exovesicles by regulating membrane lipids during the early induction culture of Chlorella, combined with TFF concentration and density gradient centrifugation. This method effectively eliminates impurities, achieving a recovery rate of over 60% and a purity of 2.56 × 10⁻⁶. 10 The particle / μg method provides an efficient, controllable, and scalable solution for the industrial-scale separation and preparation of microalgal exovesicles.

The invention discloses a photoresponsive antibacterial hydrogel, which comprises a photosensitizer, acrylamide, potassium 3-sulfopropyl methacrylate, chitosan quaternary ammonium salt, an additive and a cross-linking agent, and the structure of the photosensitizer (PD-Se) is shown in the specification. A photosensitizer (PD-Se) is added into hydrogel, the positive charge characteristic of QSA hydrogel remarkably improves the local enrichment concentration of PD-Se in thalli by enhancing the binding force with the bacterial surface, unsaturated fatty acid in a bacterial membrane lipid bimolecular layer is oxidized after photoinduced ROS is generated, guanine bases in a DNA double-helix structure are attacked at the same time, and therefore the bioactivity of the bacterial membrane lipid bimolecular layer is improved. The gel is endowed with photodynamic antibacterial ability, has an excellent sterilizing effect on escherichia coli, staphylococcus aureus and methicillin-resistant staphylococcus aureus, and can be used for preparing hydrogel dressing for wound repair.

This application relates to the field of plant genetic engineering technology, specifically disclosing the application of a sterol synthesis-related gene in the response regulation of plant salt tolerance. This application clarifies the sterol synthesis-related gene. SMT2 This study expands our understanding of the role of sterol biosynthesis in plant stress response by revealing the function of calcium signaling under salt stress; it also unveils a novel mechanism by which sterol-membrane lipid interactions regulate calcium signaling, providing a new perspective for research on plant salt stress signal transduction; furthermore, this application provides new salt tolerance gene resources. SMT2 Genes can be used for molecular breeding of salt-tolerant crops, to breed salt-tolerant crop varieties, or to develop salt-tolerant molecular markers.

The invention provides a double-layer membrane lipid droplet simulating breast milk and a construction method and application thereof, milk phospholipid and a membrane protein concentrate are separated from MFGM-10, butter or cream is used as an oil phase, and phospholipid double-layer membrane lipid droplets and / or phospholipoprotein double-layer membrane lipid droplets are prepared from the milk phospholipid and / or the membrane protein concentrate. Through detection, the steric hindrance is increased due to the existence of cholesterol in the interface of the double-layer membrane lipid droplet, the specific surface area of the double-layer membrane lipid droplet is larger, the interface contains more emulsifiers, and the long-term stability and emulsibility of the double-layer membrane lipid droplet are superior to those of a single-layer membrane lipid droplet with the same components. The double-layer membrane lipid droplet is closer to the interface structure of breast milk fat globules than the single-layer membrane lipid droplet, the long-term stability of dairy products can be improved by adding the double-layer membrane lipid droplet into the dairy products including infant formula milk powder, and more unsaturated fatty acids can be released in the digestion process.

The present application relates to a kind of high capric acid resistant genetically engineered bacteria and its construction method and application.The genetically engineered bacteria take E. coli as starting strain, knock out aaaS gene, simultaneously overexpress mlaZ gene and mlaY gene;Wherein, the Gene ID of aaaS gene nucleotide sequence is 947315;The Gene ID of mlaZ gene nucleotide sequence is 882402;The Gene ID of mlaY gene nucleotide sequence is 882401.The present application effectively strengthens the membrane lipid intensity of E. coli engineering bacteria by knocking out aaaS gene in E. coli and simultaneously overexpressing mlaZ and mlaY gene, so that genetically engineered bacteria show better robustness and higher resistance in the fermentation process of high-concentration substrate capric acid.Trans-2-decenoic acid yield can reach 1.47g / L when capric acid is added to the highest concentration of 3.0g / L, and the conversion rate is 49.13%, which significantly improves the yield.

The invention discloses a saccharomyces cerevisiae genetic engineering strain and application thereof. Based on a chassis strain SquMC13 with high squalene productivity, a sclareol synthesis route is modularly reconstructed, precursor carbon flow distribution is dynamically regulated and controlled, cell wall and membrane system steady states are adjusted, membrane lipid composition is adjusted, a cell cycle is intervened, and a product efflux system is enhanced, so that the problem of cytotoxicity caused by product accumulation is relieved to a certain extent. According to the invention, a plurality of saccharomyces cerevisiae engineering strains with high yield of sclareol are constructed, including Scl14, Scl24, Scl31, Scl38, Scl71 and Scl75, the result of the strain Scl75 is most prominent, the yield of sclareol fermented by a 250 mL shake flask is up to 4.1 g / L, efficient, stable and green biosynthesis of sclareol is realized, and a new strategy and theoretical basis are provided for microbial preparation of complex diterpenoid compounds.

The present application relates to the technical field of medicine preparation, and discloses a macrophage membrane liposome packaged metformin drug delivery system, a preparation method thereof and application in tumor treatment, which comprises a ZIF-8 carrier carrying a medical active substance of metformin hydrochloride, wherein the metformin hydrochloride is encapsulated in ZIF-8 nanoparticles, and the outer layer is packaged with tumor-related macrophage membrane liposomes; in application examples, it is found through in-vitro experiments that the killing effect of the system on human osteosarcoma cell lines is strong, the system can more effectively kill tumor cells and induce immunogenic death; compared with free metformin hydrochloride monomer drugs, the aforementioned delivery system has a more obvious inhibitory effect on tumor cells and can more effectively induce immunogenic death.

The utility model relates to medical instrument technical field provides a kind of flexible plasma patch, including first dielectric barrier layer, electrode layer, second dielectric barrier layer and medical film layer by upper to lower sequentially laminated settings, the electrode layer is suitable for connecting power module, for generating plasma, the medical film layer is suitable for contacting wound surface, the first dielectric barrier layer, the electrode layer, the second dielectric barrier layer and the medical film layer are all correspondingly provided with through-hole. The utility model is through setting corresponding through-hole, on the one hand make outside air pass through through-hole and enter wound surface, increase the generation amount of active particle, effectively improve the efficiency of sterilization and promote wound healing;On the other hand, the ozone generated by electrode layer discharge flows out from through-hole, thereby reducing ozone accumulation, avoiding the tissue oxidation stress caused by ozone accumulation, leading to cell membrane lipid peroxidation.

The invention provides a fruit tree dormant branch cold resistance comparison method, and relates to the technical field of fruit tree cultivation, and the method comprises the following steps: alternately executing polarization mode operation and impedance mode operation on the same detection site of a fruit tree dormant branch; wherein when the polarization mode operation is carried out, a first wave band laser and a second wave band laser are emitted to a detection site at a preset incident angle, and a transmission light intensity data set is acquired; when impedance mode operation is carried out, sweep-frequency current is injected into a detection site, and an impedance component data set of a characteristic frequency point is collected; calculating the intercellular ice crystal density according to the transmission light intensity data set; calculating a membrane lipid phase shift angle according to the impedance component data set; when the intercellular ice crystal density is greater than an activation threshold value, calculating an ice crystal-membrane lipid synergy ratio based on the intercellular ice crystal density and a membrane lipid phase shift angle; calculating a cold-resistant synergy index according to the intercellular ice crystal density and the change rate of the membrane lipid phase shift angle; and judging the cold resistance grade according to the cold resistance synergy index value.

The invention belongs to the technical field of probiotics, and particularly discloses a method for improving the anti-drying capacity of probiotics and application of the method. The preparation method comprises the following steps: S1, inoculating probiotics into a culture medium, adding a fatty acid precursor for culturing, and centrifugally collecting thalli; and S2, washing the thalli collected in the step S1, resuspending the thalli in a protective agent solution, performing spray drying, and performing magnetic stirring in the drying process to obtain dry bacterial powder. The invention discloses a method for improving the anti-drying capacity of probiotics and application of the method, fatty acid precursors are exogenously supplemented in the culture process, the cell membrane lipid composition of the probiotics can be effectively regulated and controlled, the anti-spray-drying capacity of the probiotics is remarkably enhanced, and the key problem that the survival rate of the probiotics is low in the traditional spray drying process is solved.

The invention belongs to the technical field of cell viability on-line discrimination, and particularly relates to a mass spectrum-based cell viability measurement method and a mass spectrum-based cell viability measurement system. The invention provides a label-free endogenous metabolite-based cell viability measurement scheme and a label-free endogenous metabolite-based cell viability measurement system, aiming at the defects that the prior art depends on an exogenous label and cannot be synchronously carried out on line with single cell mass spectrometry. The method comprises the following steps: acquiring continuous single cell metabolome data through single cell electrospray ionization mass spectrometry, identifying a single cell event and a time window by taking ubiquitous membrane lipid as a first characteristic ion, extracting an endogenous metabolite reflecting cell activity in the window as a second characteristic ion, and calculating a signal-to-noise ratio; generating an activity label according to a preset threshold value and counting the cell viability of the group, wherein the system correspondingly comprises a data acquisition module, an event identification module, a marker extraction module, a threshold value judgment module and a viability counting module. According to the invention, synchronous, on-line and label-free accurate measurement of the cell viability in single cell metabonomics analysis is realized, and exogenous interference and data deviation are effectively avoided.

To provide a method for promoting regeneration of a tissue.SOLUTION: A method for promoting regeneration of a tissue of a subject includes a) a step of administering a composition to the subject, where the composition includes a heterogeneous mixture of composites including: i. a first composite containing first cyclodextrin molecules and Wingless (Wnt) protein covalently bonded to first cell membrane lipid; and ii. a second composite containing second cyclodextrin molecules and Hedgehog (Hh) protein covalently bonded to second cell membrane lipid.SELECTED DRAWING: Figure 1

The application discloses a preparation method of a flaky magnetic material for enriching exosomes. The flaky Fe3O4 magnetic material with high specific surface area is taken as a starting point, and a surface of the flaky Fe3O4 magnetic material is coated with silicon dioxide to enhance dispersibility and chemical stability of the flaky Fe3O4 magnetic material, and to provide controllable silicon hydroxyl reaction sites for subsequent surface chemical modification. On this basis, amino active groups are introduced to the surface of the magnetic material through 3-aminopropyl triethoxysilane (APTES), and DSPE-SS-PEG2000-CHO containing a disulfide bond structure is further covalently connected to the surface of the magnetic material. Due to a membrane lipid insertion mechanism, non-antibody-dependent capture of a lipid bilayer exosome can be realized. The flaky magnetic material disclosed by the application shows universality and high affinity advantages, and can realize high affinity capture of a lipid structure of an exosome.

The invention concerns antimicrobial compositions comprising free fatty acids emulsified with membrane lipids or hydrolysed derivatives thereof, and pharmaceutical formulations comprising same. The compositions can be used in the treatment of prophylaxis of microbial infections. They can also regulate the rate of blood clotting rendering them suitable for incorporation in catheter locking solutions and for use in wound care.

The present invention relates to the technical field of skin care products, and provides a biomimetic sebum membrane lipid nanoparticle with a repairing function, a preparation method, an application, and a product. The biomimetic sebum membrane lipid nanoparticle is composed of the following ingredients by weight: wax ester 8%-10%, triglyceride 15%-20%, squalane 3%-6%, sterol 0.1%-2%, ceramide 0.05%-0.25%, lecithin 1%-5%, antioxidant 0.1%-1%, polyol 5%-22.5% and the balance water; the weight content of phosphatidylcholine in the lecithin is ≥45%, and the particle size of the biomimetic sebum membrane lipid nanoparticle is ≤200nm. The sterol added in the present invention has a synergistic effect with the lecithin, so that the biomimetic sebum membrane lipid nanoparticle has a better repairing effect, and adding it to cosmetics can more effectively promote the transdermal penetration and absorption of water-soluble active substances in the formula.

The application discloses a kind of L-lysine high-yield production strain modification method and its application, belong to the field of bioengineering technology.The application obtains recombinant bacteria by knocking out CbrB gene and / or CbrC gene on the genome of escherichia coli, and the yield and conversion rate of L-lysine can be improved by fermenting the recombinant bacteria, and the mortality in the fermentation process of strain can be reduced.The application has important application value.The application finds through experiment that the influence of the two genes on escherichia coli has synergistic effect, L-lysine hydrochloride has osmotic pressure stress effect on strain, and silencing related gene protein can change membrane lipid composition (increase the proportion of unsaturated fatty acid), maintain membrane fluidity.Improve the tolerance to lysine hydrochloride, reduce the mortality in later fermentation stage.

This invention provides a method, program, and apparatus for more easily designing delivery carriers suitable for target cells. [Solution] A method for predicting delivery carrier composition comprises the steps of preparing a delivery carrier prediction tool constructed by machine learning using a delivery carrier composition dataset of multiple types of delivery carriers exhibiting different component compositions, a non-target cell membrane composition dataset of multiple types of cells other than target cells relating to cell membrane lipid composition, and a non-target cell delivery amount dataset of multiple types of delivery carriers relating to the amount of delivery substances delivered to multiple types of cells other than target cells as training datasets, and using the delivery carrier prediction tool to obtain a delivery carrier composition that satisfies a target value based on the cell membrane lipid composition of target cells.

The invention provides an extraction method of fructus cannabis oil and the fructus cannabis oil, and particularly relates to the technical field of oil extraction. The extraction method comprises the following steps: adding fructus cannabis powder into an ionic liquid for soaking, carrying out ultrasonic extraction, and finally separating to obtain the fructus cannabis oil, wherein the ionic liquid is formed by choline derivatives and polybasic organic acid. According to the extraction method, the extraction efficiency and quality of the fructus cannabis oil are remarkably improved by adopting an ionic liquid and ultrasonic synergistic assistance technology. The ionic liquid effectively destroys a lignin-hemicellulose composite structure in fructus cannabis cell walls through hydrogen-bond interaction, increases the fluidity of a cell membrane lipid layer, forms micro-jet by combining with a cavitation effect generated by ultrasonic waves, promotes permeation of the ionic liquid and synergistically dissociates a protein-lipid complex, and realizes efficient release and dissolution of grease. And the used ionic liquid is non-toxic, degradable and recyclable, so that the production cost is remarkably reduced.