6 results about "Cell integrity" patented technology

The utility model provides a multi-stage filter tube for pretreatment of a cell suspension, which is characterized in that each second single tube body serves as a middle tube body and is of a transparent cylinder structure, an external thread structure is arranged at the port of the top end of each second single tube body, and an internal thread structure matched with the external thread structure is arranged at the port of the bottom end of each second single tube body; every two adjacent single pipe bodies can be connected and installed through matched thread structures, and connecting pipe bodies which are sequentially arranged from top to bottom and provided with multiple stages of filtering hole diameters which are reduced step by step are formed. The first single tube body is used as the uppermost tube body; the third single pipe body is used as the lowermost pipe body; and the air pressure adjusting device is communicated with the first single pipe body. According to the utility model, through a multi-stage filtering structure, the aperture is reduced stage by stage, so that the filtering efficiency is improved, and the cell loss and the impurity residue are obviously reduced; the cell integrity is ensured by adopting lower pressure, the damage to the cells caused by overhigh impact force is effectively avoided, and meanwhile, the impurities are ensured to be fully removed.

A low-cell-breakage-rate centrifugal tube for preparing platelet-rich plasma comprises a tube body, an upper tube cover is arranged on the tube body, an input channel is formed in the upper tube cover, a guide-in tube is arranged at the end, facing the tube body, of the input channel, and the guide-in tube is arranged obliquely towards the inner tube wall of the tube body; the upper pipe cover is further provided with a plurality of taking and conveying channels. The arrangement mode that the ingress pipe inclines towards the pipe body is adopted, drainage can be conducted through the inner wall of the pipe body, a very good buffering effect is achieved, damage to cells in the transferring process is avoided, due to the fact that the ingress pipe is short and does not make contact with blood and blood cells in the centrifugal process, damage to cells related to the catheter cannot be caused, and the cell transferring efficiency is improved. Therefore, the integrity of cells prepared from the obtained platelet-rich plasma is ensured, and the activity and clinical effect of the PRP are improved.

The present disclosure provides compositions and methods related to nucleic acid nanopore sensors. In particular, the present disclosure provides nucleic acid sensors that function as nanopores responsive to specific target nucleic. Binding of a target nucleic acid to the complementary toehold domain of the sensor leads to an increase in the size of the nanopore channel in a cell membrane, allowing for the entry or exit of various molecules such as fluorophores. These novel nucleic acid nanopore sensors enable lysis-free sensing, which maintains cell integrity and allows for real-time monitoring of target nucleic acids.

The present application belongs to the field of biotechnology, and particularly relates to a kind of production alpha-ketoglutaric acid recombinant escherichia coli engineering strain and its construction method and application.The present application engineering strain A09 can realize high-efficiency catalysis to high-concentration L-glutamic acid sodium without adding any membrane breaker such as Triton X-100.Under the condition of high-intensity catalysis for 6 hours, the cell membrane structure of the bacterial cell is kept intact without obvious damage.This characteristic completely eliminates the problems such as cell toxicity, product purification complexity, cost increase and process safety reduction caused by the use of surfactant in traditional methods, realizes the perfect unity of "cell integrity" and "high catalytic efficiency", and provides an unprecedented technical solution for the large-scale, industrialized and low-cost production of alpha-ketoglutaric acid.

The invention relates to the technical field of cell culture, in particular to a cell in-vitro anoxic culture device which comprises a transparent sealing box, a cover plate and an inflation sealing ring, a sealing assembly is arranged on one side of the transparent sealing box, a replacement assembly is arranged on one side of the transparent sealing box, and a humidification assembly is arranged on one side of the transparent sealing box; the sealing assembly comprises an inflation sealing ring fixedly connected to the interior of the transparent sealing box, one side of the outer surface of the transparent sealing box is fixedly connected with an air extracting pump, an air outlet pipe is arranged in the air extracting pump, and the end, away from the air extracting pump, of the air outlet pipe is arranged in the inflation sealing ring; the invention aims to provide a cell in-vitro hypoxia culture device to solve the problems that a conventional culture device mostly adopts a static rubber sealing ring, the fitting degree cannot be adjusted along with the change of pressure in a box, negative pressure in the box is easily caused after air exhaust, a cell culture dish cover is tightly sucked, the osmotic pressure of a culture medium is changed, and the cell culture efficiency is low. And even the integrity of nerve astroglia cells is damaged.

The invention belongs to the technical field of biology, and particularly relates to a recombinant escherichia coli engineering strain for producing alpha-ketoglutaric acid as well as a construction method and application of the recombinant escherichia coli engineering strain. The engineering strain A09 disclosed by the invention can realize efficient catalysis of high-concentration L-sodium glutamate under the condition that any membrane rupture agent such as Triton X-100 and the like is not added at all. And in a high-strength catalysis process as long as 6 hours, the cell membrane structure of the thallus is kept complete and is not obviously damaged. According to the characteristic, a series of problems of cytotoxicity, complex product purification, cost increase, process safety reduction and the like caused by use of a surfactant in a traditional method are thoroughly abandoned, and perfect unification of cell integrity and high catalytic efficiency is realized; and an unprecedented technical solution is provided for large-scale, industrial and low-cost production of alpha-ketoglutaric acid.