Cell freezing solution for preparing dry tumor cell quality control and application of cell freezing solution

Abstract

The invention discloses a cell freezing solution for preparing a dry tumor cell quality control and application of the cell freezing solution. According to a formula, the cell freezing solution is prepared by adding 5-25% of protein, 3-15% of a cell stabilizing agent A, 1-10% of a cell stabilizing agent B and 0.01-0.1% of a bacteriostatic agent into PBS (phosphate buffer solution). The cell freezing solution successfully solves the problems that a cell integrity rate after freeze-drying is very low or active ingredients of cell membrane structural proteins and cell contents cannot be reservedwell in the existing cell freeze-drying technology. A preparation method of the dry tumor cell quality control directly uses human-derived tumor cell strains containing cell membrane structures and contents to prepare the quality control; the quality control is derived from human tumor cells and has the same characteristics as those of similar tumor cells in clinical liquid biopsy detection samples; and the quality control prepared by the method is applicable to the quality control of clinical liquid biopsy and can promote extensive popularization and application of the clinical liquid biopsy.

Description

technical field [0001] The invention belongs to the field of biological detection reagents and relates to a cell freezing solution for preparing dry tumor cell quality control products and an application thereof. Background technique [0002] "Liquid biopsy technology" is to capture free-moving cancer cells or cancer DNA from body fluids (blood, urine or pleural fluid) to obtain information about small tumors or latent tumors. This will allow doctors to monitor cancer patients' response to treatment, detect early signs of recurrence, and even detect early signs of cancer. [0003] Now many companies have accelerated the speed of research and development of related technologies. Established biotech companies such as Qiagen, Roche, and Illumina, as well as startups such as Guardant Health and Foundation Medicine, are racing to commercialize liquid biopsy technology and plan to use it in the clinic in the next few years. [0004] The quality control of the liquid biopsy clini...

AI Technical Summary

Problems solved by technology

For example, in the process of cooling and rewarming, cells are extremely susceptible to "solution damage" or "intracellular ice damage" caused by factors such as solution freezing, melting, and changes in solution osmotic pressure, resulting in a low recovery rate. [1]

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