50 results about "Sclareol" patented technology

The present invention provides a method of producing sclareol, said method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, said method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human host organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.

The invention discloses a method for constructing a high-yield sclareol engineering strain by a synthetic biology methdo, and an application thereof. The construction method for the strain relates to express plant sourced key enzymes for synthesizing sclareol, wherein the key enzymes comprise a pyrophosphoric acid ladanum enediol ester synthase and a sclareol synthase; and overexpress a combination of one or more yeast endogenous enzyme-based genes, wherein the enzyme-based genes comprise a key enzyme of a mevalonic acid approach-(hydroxymethyl) coenzyme A reductase, and two enzymes of an isopentenylpyrophosphate approach-a farnesyl pyrophosphate synthase and a geranylgeranyl diphosphate synthase. The method can efficiently produce sclareol, with a shake flask fermentation level reaches 9 mg/l, and has industrial application prospects.

The invention discloses a cream for anti-oxidation and improvement of the human immunity and a preparation method thereof, the invention is the cream which is prepared by taking metallothionein, coenzyme-Q10 chelate lipoic acid, sclareol, chamomile, L-carnitine, rosemary oil, cimicifuga racemosa, dextran, Kamm, jojoba oil, distilled water and collagen protein as raw materials, the cream has the advantages that the effects can be improved by a plurality of times of the effects before the chelation by the organic compound formula of a plurality of anti-oxidation natural organisms for improving the human immunity, such as, MT, coenzyme-Q10 chelate and so on, thus having good improving effects on swollen welling-abscess, rhinitis and asthma which are caused by the damages of the immune system and having great eliminating effect on the prevention of the tumors; furthermore, the skin can be moisturized, whitened and replenished with water by smearing the cream of the invention.

The epoxy sclareol is synthesized with sclareol as main material, hydrogen peroxide solution as oxidant, and water and/or organic solution of transition elements, especially VB or VIB transition elements as the catalyst for activating hydrogen peroxide. Inside the homogeneous or two-phase water-organic solvent system, there is also of kind of phase transfer catalyst, so as to epoxidize sclareol into epoxy sclareol and to obtain epoxy sclareol through conventional separation and purification. Compared with available technology, the present invention uses cheap and clean hydrogen peroxide aqua as oxidant and has the advantages of simplicity, cleanness, high efficiency, high safety and low cost.

The invention discloses a preparation technology of sclareol, which adopts a molecular still with a distance of 2cm to 5cm between an evaporating surface and a condensing surface. The technology of the invention is characterized in the operation under the following technological conditions is carried out and comprises the following steps of: operating pressure for the still is 1Pa to 150Pa, distilling temperature is 100 DEG C to 400 DEG C, cooling temperature is minus 8 DEC G to minus 15 DEG C, rotating speed of a film applicator is 10rpm to 300rpm, remains of the first-step distillation are deemed as raw materials for a next distillation and are taken 2 times to 3 times of distillation, temperature contrast between the evaporating surface and the condensing surface is kept as 175 DEG C to 275 DEG C for each time of distillation, so as to completely separate and extract sclareol from clary sage concrete and to ensure the yield of sclareol to reach 98 percent. The technology of the invention has advantages of simple technology, short operating time, nonuse of solvent, nonproduction of other high boiling materials and pollution-free. The technology of the invention has high efficiency on extracting the sclareol, high product yield and high benefit, and is applicable to large-scale production of the sclareol.

The invention discloses a method for processing aromatic tea seed oil. The method comprises the following steps: S1, selecting high-quality tea seeds, drying with microwave, threshing, grinding, mixing with a mixed solution A, and performing enzymatic hydrolysis to obtain a material B; S2, adding salvia sclarea essential oil and sclareol into the material B, and sufficiently mixing to obtain a material C; S3, squeezing the material C to obtain press cake and tea seed crude oil; and S4, filtering the tea seed crude oil, removing wax, adding modified sodium-montmorillonite, stirring, standing, and filtering to obtain the aromatic tea seed oil. According to the method, high-temperature steaming or frying is not required in the preparing process, and no toxic or harmful substance is generated. The method has the advantages of less loss of tea seed nutrition, high oil yield, high tea seed oil quality, excellent oxidation stability and health-protection effect, and can be used for prolonging the preservation period of tea seed oil.

The invention relates to a method for preparing fragrant perilla alcohol by using a fragrant perilla alcohol extract. The invention principally relates to the following steps: the fragrant perilla alcohol extract is placed into an organic solvent; saponification is carried out under the condition of temperature of 50 to 130 DEG C and no existing inorganic base. The obtained organic phase is washed by the buffer solution with a pH value of 5.0 to 8.0 and is then crystallized under the temperature of minus 20 to 0 DEG C for obtaining the target product. Compared with the present method of preparing the fragrant perilla alcohol by using the fragrant perilla alcohol extract, the invention has the advantages that the preparation process is simple and is friendly with the environment (the solvent used in the preparation process can all be recycled and reused), and the obtained product has a high purity (the content of the fragrant perilla alcohol is more than 93 wt percent).

The invention relates to a method for biologically synthesizing sclareolide. The method comprises the steps of taking a yeast culture medium and separately adding sclareol and a light white cryptococcus protoplast fluid into the yeast culture medium to obtain a mixture, wherein the sclareol accounts for 3-8% of the total mass of the yeast culture medium, and the light white cryptococcus protoplastfluid accounts for 4-10% of the total mass of the yeast culture medium; placing the mixture in a glass vessel, and then shaking the vessel for fermentation for 10 days on a shaking table at the temperature of 23-28 DEG C and the speed of 140-180 r/min to obtain sclareolide. According to the method, a microorganism conversion method is utilized, and sclareol is used as a substrate and converted bylight white cryptococcus to prepare sclareolide, so that the problems that a synthesis technology process of sclareolide based on a chemical method is environmentally unfriendly, complicated in step,low in safety and the like are solved, and a new way is provided for synthesizing sclareolide.

A method for the identification of a composition useful in the treatment of an overweight or obese person to reduce the person's body mass index is provided which comprises obtaining an extract of an ethnobotanical plant, and evaluating the activity of the extract in an assay selected from the group consisting of a lipolysis assay, an assay that measures the amount of glycerol introduced by a cell into a suspension medium of the cell, an adipocyte differentiation assay, an assay that measures the level of the enzyme glycerol-3-phosphate dehydrogenase, an assay that measures the inhibition of differentiation of preadipocytes to adipocytes, an assay that measures the accumulation of lipid in an adipocyte, an assay that measures the de-differentiation of adipocytes into preadipocytes, and combinations thereof. Sclareol and sclareol-like compounds and sclareolide and sclareolide-like compounds are identified as useful in the treatment of overweight and obese conditions and disorders and conditions associated with adipose tissue abnormalities. These compounds can be formulated for oral administration.

The present invention belongs to the technical field of livestock and poultry breeding and particularly relates to an immunity enhancing feed for pigs. The feed for the pigs consists of corns, soybean meal, monopterus albus bones, egg shells, B12, eichhornia crassipes, skim rice bran, phymatopsis engleri, polygonum aviculare, compound vitamins, fructo-oligosaccharides, duchesnea indica, sclareol, cucumis melo var. agrestis, zinc sulfate, copper sulfate and pumpkin seeds. The feed for the pigs is full in nutrition and good in palatability, can increase appetite of the pigs, promotes intestinal health, promotes digestion, strengthens immune capacity of the pigs, improves disease resistance, reduces the prevalence, and is safe and free of hormones.

The invention relates to the technical field of medicinal material cultivation, in particular to a cultivation method for cinnamon. The cultivation method comprises the following steps: (1) selection and preparation of a land; (2) breeding and seedling growth: selecting intact and full seeds, immersing the selected seeds with seed soaking solution for 30 to 40 minutes, fishing out the seeds and sowing the seeds by adopting a drilling method, wherein the seed soaking solution is prepared from sclareol, sodium diacetate, calcium propionate, hemsleya roots, biogas slurry, human feces, plant ashes, sheep urine, fresh apricot flesh, potassium chloride, ammonium sulfate, chicken manure and lithocarpus pachylepis; (3) field management; and (4) nutrient and water management. According to the cultivation method for the cinnamon, the germination rate of cinnamon seeds is improved, the germination time of the cinnamon seeds is shortened and the yield of the cinnamon is improved.

The invention discloses a strain combination for producing sclareol, an application and a method for producing sclareol. The strain combination for producing sclareol is composed of saccharomyces cerevisiae engineering bacteria and fungi with the preservation number of ATCC 20624, and the saccharomyces cerevisiae engineering bacteria externally express lysimachidonediol pyrophosphate synthase andsclareol synthase. Compared with the prior art, the saccharomyces cerevisiae has the remarkable advantages that 1, a precursor GGPP for synthesizing sclareol can be provided by a metabolic pathway ofthe saccharomyces cerevisiae, a microbial synthesis method of the sclareol can be realized by introducing two exogenous genes, and a raw material is provided for producing sclareol; 2, in a co-culturesystem, the fungus ATCC 20624 takes sclareol generated by yeast as a substrate and converts the sclareol into sclareol, so that de novo biosynthesis of sclareol can be realized, and 3, the fungus ATCC 20624 converts the sclareol, so that the yeast can be further promoted to produce the sclareol.

The invention discloses a method for extracting and purifying sclareol from sclareol fermentation liquor. The method comprises the following steps: (1) centrifuging fermentation liquor to obtain thalli, and drying and crushing the thalli; (2) extracting sclareol in the thalli by using an organic solvent under the assistance of microwaves to obtain an extract; and (3) extracting the extract with an acetone aqueous solution, and recrystallizing to obtain sclareol. After the fermentation liquor is extracted and purified by the method, the purity of the obtained sclareol can reach 95.11%. The recovery rate of sclareol in fermentation liquor can reach 63.95%. The extraction and purification process has few steps, the operation is simple, the used solvent can be recycled, and the method is environment-friendly.

The invention discloses an application of a composition containing cryptolepine analogues and small molecule terpene compounds in prevention and treatment of plant diseases. The bactericidal composition comprises the following effective components: cryptolepine, neocryptolepine, sclareol, sclareolide, (+/-)-limonene, citronellol, ambergris, perilla alcohol, perillaldehyde, 2-hexenal, 4-terpene alcohol, terpilenol, alpha-terpilenol, camphor, citral, geraniol, menthol, nerolidol, eudesmol, curcumenol, cedrol and beta-ionone. The bactericidal composition disclosed by the invention can be used for preventing and treating plant diseases caused by rhizoctonia solani, Rape sclerotinia stem rot bacteria, botrytis cinerea, fusarium graminearum, magnaporthe oryzae and phytophthora capsici. The components are all natural products, have the characteristics of low toxicity, low residue, environmental compatibility, difficult generation of drug resistance and the like, can cope with occurrence of drug resistance of diseases and reduce the drug cost, and are beneficial to comprehensive management of the diseases.

The invention discloses a preparation process of sclareol, and relates to the technical field of plant extraction. According to the preparation process of sclareol, sclareol is used as a raw material, an organic solvent is used as a solvent, and after continuous countercurrent extraction and solvent recovery, a sclareol product is prepared. According to the preparation process of sclareol disclosed by the invention, while the advantages of various traditional processes are retained, the following advantages which cannot be achieved by the traditional processes are created: the extraction speed is high, the effective components are fully extracted, the extraction yield is high, the solvent consumption is low, the feed liquid concentration is high, evaporation and concentration are reduced and the like; the extraction time is short, materials are extracted in a mild dynamic environment, the heating temperature is low, effective components are less damaged, the content of impurities in feed liquid is low, and the process belongs to continuous production and is high in treatment capacity.

The invention provides a method for separation purification of sclareolide from synthetic sclareol composites by using molecular distillation device for operation under pressure of 0.01-100.0Mpa, evaporative temperature 50-200 deg. C, cool-down temperature -15-80 deg. C, temperature difference between the evaporator and condenser 30-215 deg. C, the agitating speed 10-1000 rp.

The invention discloses a production technology of sclareolide synthesized from sclareol. The technology successively comprises the following steps of: cracking station, hydrolysis station, acidifying station, ring closing station and purification so as to obtain 95% of sclareolide. An oxidizing agent used in the method is a hydrogen peroxide composite oxidizing agent and a catalyst is heteropolyacid salt. The invention has the following beneficial effects: by the use of the composite oxidizing agent and the catalyst heteropolyacid salt for reaction instead of using a hydrogen peroxide single oxidizing agent, conversion rate is raised by 22-25%, reaction time is greatly shortened, and the reaction time is reduced from more than 20 hours to less than 10 hours.

The invention relates to a preparation method of sclareolide, comprising the following steps of: (a). oxidizing sclareol to obtain a crude sclareolide product; and (b) crystallizing the crude sclareolide product obtained in the step (a) to obtain a pure sclareolide product. The preparation method is characterized in that sclareolide is recovered and processed from crystallization mother liquor obtained in the step (b), and the obtained sclareolide is mergered into the step (b) and crystallized and purified together with the synthesized crude sclareolide product. The invention adopts the environment-friendly method with simple operation, realizes the purpose of recovering sclareolide from the sclareolide crystallization mother liquor, improves the total recovery of the sclareolide and establishes a solid foundation for improving the commercial value of the sclareolide.

The invention discloses an anti-acne and freckle-removing facial mask, which comprises components such as linden bee pollen enzyme, sclareol, picrosteroid, glycerin, propylene glycol, betaine, sodium hyaluronate and the like. In the present invention, the compound use of linden bee pollen enzyme, sclareol and picrosteroid can exert the best skin care effect: linden tree bee pollen enzyme can promote cell metabolism and accumulate harmful substances, thereby reducing cell damage and pigmentation deposition; bitter steroids can scavenge free radicals and reduce cell structure damage caused by lipid peroxidation; sclareol can further inhibit microbial reproduction. The ingredients act synergistically to achieve good anti-acne and anti-pigmentation effects; and can improve facial microcirculation, promote skin repair, and maintain skin health: the mask of the present invention is high in safety, mild and non-irritating, and has a good Permeable, can deeply replenish and lock water, and deeply nourish the skin.