53 results about "Sclareol" patented technology

The invention belongs to the technical field of bioengineering, and discloses a recombinant yarrowia lipolytica capable of efficiently and rapidly producing sclareol, which is characterized in that optimized sclareol synthase related coding genes ScLPPS, ScSCS, GGPPS1, GGPPS2, ERG20 and tHMGR coding genes are inserted into a yarrowia lipolytica genome; inserting optimized pentadienol utilization pathway coding genes such as IPK, THIM and IDI coding genes; and inserting an optimized ATP: citrate lyase coding gene ACLY to obtain the ATP: citrate lyase coding gene ACLY. The recombinant yarrowia lipolytica is based on knockout of a yarrowia lipolytica strain responsible for coding a non-homologous recombination gene ku70, so that the homologous recombination capability of the yarrowia lipolytica strain is enhanced, gene integration is realized through the homologous recombination function of the yarrowia lipolytica, the genetic stability of the introduced gene can be greatly improved, and the recombinant yarrowia lipolytica strain has a good application prospect. The construction method of the recombinant yarrowia lipolytica strain is efficient and simple to operate.

The invention belongs to the technical application field of microbial metabolic engineering and synthetic biology, and particularly relates to a pichia pastoris engineering strain for high-yield sclareol as well as a construction method and application of the pichia pastoris engineering strain. The method comprises the following steps: constructing a biosynthetic pathway of sclareol in a host strain, and optimizing an intracellular mevalonic acid metabolic pathway and a central metabolic pathway to obtain an engineering strain A; the host strain is pichia pastoris; or overexpressing or knocking out a metabolism regulation factor in the obtained engineering bacterium A to obtain an engineering bacterium strain B; or, in the obtained engineering bacterium B, a synthetic pathway is targeted to peroxisome and optimized by adopting a cell region compartment strategy, and an engineering bacterium strain C is obtained. The invention provides a pichia pastoris chassis cell for synthesizing a diterpenoid sclareol and an engineering strain for synthesizing sclareol. By introducing and optimizing the expression of a sclareol synthesis pathway, the fermentation yields of sclareol in shake flask batch and bioreactor batch fed-batch fermentation are respectively 631.6 mg / L and 10.5 g / L.

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The invention belongs to the technical field of biosynthesis, and particularly relates to a strain for producing sclareol as well as a construction method and application of the strain. In order to solve the problem of microbial synthesis of sclareol, phosphoketolase in an NOG pathway is mutated and optimized, phosphoketolase NaXpk from nanoarchaea archaeon is subjected to site-directed mutagenesis, a sclareol synthesis pathway is constructed in an escherichia coli host, the influence of NaXpk before and after mutation on the yield of sclareol is compared, and the yield of sclareol is improved. The S472F mutant and the S472Y mutant are determined, the S472F mutant and the S472Y mutant are applied to a sclareol production path, the sclareol yield of the finally obtained production strains can reach 623.3 mg / L and 655.2 mg / L, and the effect is remarkable.

The invention discloses application of sclareol in preparation of drugs for resisting porcine reproductive and respiratory syndrome virus, and belongs to the field of veterinary drugs. According to the invention, Marc-145 cells are taken as host cells, a plurality of porcine reproductive and respiratory syndrome virus strains are selected for infection, and the antiviral performance of sclareol is tested; experimental results show that 30-50 [mu] M sclareol has no obvious toxicity to host cells, and can significantly inhibit infection of different porcine reproductive and respiratory syndrome virus strains in the host cells. The sclareol has good activity of resisting porcine reproductive and respiratory syndrome virus infection, and the sclareol is a medicine with a natural source, has the advantages of safety, few side effects, low metabolic residue and no pollution, and provides a reliable basis for treatment, prevention and control of porcine reproductive and respiratory syndrome.

The present invention relates to the technical field of aromatherapy, and specifically discloses a compound essential oil for alleviating anxiety, its preparation method and application. A compound essential oil for alleviating anxiety comprises raw materials in the following parts by mass: 50 - 65 parts of sweet almond oil, 12 - 16 parts of French lavender oil, 0.5 - 1.2 parts of Xinjiang lavender oil, 6 - 9 parts of cypress oil essential oil, 1 - 3 parts of Virginia cypress oil, 0.1 - 0.3 parts of complete ylang-ylang oil, 0.7 - 1.3 parts of Xinjiang sclareol oil, 2 - 4 parts of bergamot oil, and 2 - 4 parts of eucalyptus oil. The preparation method of the compound essential oil for alleviating anxiety is to mix the raw materials to obtain the compound essential oil. The present application also provides an application of the compound essential oil for alleviating anxiety in aromatherapy products. The compound essential oil for alleviating anxiety in the present application has obvious effects of alleviating anxiety emotions and improving sleep quality.

The invention belongs to the field of bioengineering, and particularly relates to a bacillus for degrading cis-abienol to generate sclareol and application of the bacillus. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.30672, and the strain is classified and named as bacillus sp. The bacillus disclosed by the invention can convert a Labdan substance cis-abienol into an ambergris substance sclareol, and is used for fermenting tobacco leaves, so that the tobacco leaves are fermented, and the yield of the tobacco leaves is increased. 41.9% or above of cis-abienol can be degraded within 48 h, the quality of tobacco leaves can be improved, and the sensory quality of cigarettes can be improved.

The invention provides an engineering bacterium and a method for synthesizing sclarediol. Wherein the engineering bacterium comprises a sclareone synthase gene and a Bayer-Villiger monooxygenase gene, and the sclareone synthase comprises: (a1) a protein having any sequence in SEQ ID NOs: 1-25, or (a2) a protein having any sequence in SEQ ID NOs: 2-25, or (a3) a protein having any sequence in SEQ ID NOs: 2-25; (a2) protein which has more than 70% of homology with the amino acid sequence limited in (a1) and has sclareone synthase activity; the Bayer-Villiger monooxygenase comprises: (b1) a protein having any one sequence in SEQ ID NOs: 26-50, or (b2) a protein having any one sequence in SEQ ID NOs: 26-50; (b2) is protein which has more than 70% of homology with the amino acid sequence limited in (b1) and has Bayer-Villiger monooxygenase activity. The method can solve the problem of difficulty in efficient biosynthesis of sclarediol in the prior art, and is suitable for the field of synthetic biology.

The invention provides application of sclareol in preparation of a medicine for preventing and treating varicella-zoster virus infection. A series of biological experiments show that the sclareol can obviously relieve the cytopathic effect caused by VZV infection under the concentration without obvious cytotoxicity; the gene expression quantity of VZV characteristic genes including an immediate early gene ORF62, an early gene ORF21 and a late gene ORF68 and the expression of an early protein IE62 and a late protein gE can be remarkably inhibited, so that the effect of resisting VZV infection is achieved. The results show that sclareol has obvious prevention and treatment effects on VZV, has small toxic and side effects, is safe and effective, and has an application prospect in preparation of drugs for treating and / or preventing VZV.

The invention relates to the technical field of genetic engineering, in particular to recombinant yarrowia lipolytica with high yield of sclareol as well as a preparation method and application of the recombinant yarrowia lipolytica. By analyzing the metabolism principle of yarrowia lipolytica, a key metabolic pathway for producing sclareol is regulated and controlled, eight recombinant strains are designed and constructed, CJ-8 overexpresses a tPaGGPPS gene, a four-copy SsSL gene, an ERG19 gene, an ERG13 gene, an ERG12 gene, an ERG8 gene and a CAT2 gene, and a DGA2 gene is knocked out at the same time. Compared with an initial strain and other seven recombinant strains, the CJ-8 can be fermented to synthesize sclareol, the synthesis capability is remarkably improved, and the sclareol yield of the CJ-8 reaches 2747.50 mg / L. In conclusion, when the recombinant yarrowia lipolytica provided by the invention is used for fermentation production of sclareol, the yield of sclareol can be greatly improved.

The present invention belongs to the field of microbial metabolic engineering and synthetic biology technology applications, and particularly relates to a Pichia pastoris engineered strain that produces high sclareol production, as well as its construction method and application. A sclareol biosynthesis pathway is constructed in a host strain, and the intracellular mevalonate metabolic pathway and the central metabolic pathway are optimized, thereby obtaining an engineered bacterial strain A; the host strain is Pichia pastoris; or, metabolic regulatory factors are overexpressed or knocked out in the above-obtained engineered bacterial strain A, thereby obtaining an engineered bacterial strain B; or, in the above-obtained engineered bacterial strain B, a cell compartmentalization strategy is used to target the synthesis pathway to the peroxisome and optimize it, thereby obtaining an engineered bacterial strain C. The present invention provides a Pichia pastoris chassis cell for synthesizing the diterpenoid compound sclareol and an engineered strain for synthesizing sclareol; by introducing and optimizing the expression of the sclareol synthesis pathway, the sclareol yields in shake flask batch fermentation and bioreactor batch fed-batch fermentation reach 631.6 mg / L and 10.5 g / L, respectively.

The invention provides a preparation method of sclareolide and a catalyst, and belongs to the technical field of organic catalysis. The method comprises the following steps: dissolving sclareol by using a first solvent, adding a catalyst, adjusting the pH value of the solution under microwave conditions, dropwise adding hydrogen peroxide, heating and stirring for reaction, separating the catalyst, reusing, extracting the product by using a second solvent, and heating and refluxing to obtain sclareolide; the catalyst is imidazolyl ionic liquid / polyethylene glycol / maleic acid modified graphene oxide loaded with vacancy heteropolyacid salt. According to the preparation method of sclareolide, microwave-assisted reaction is adopted, so that the product yield of sclareolide is greatly improved. According to the invention, two mutually compatible reaction systems of alcohol and water are adopted, so that the environmental protection property of the process is greatly improved, and meanwhile, under the action of the catalyst, the method has a phase catalytic transfer effect, the reaction efficiency is improved, the reaction condition is mild, the product yield is high, and the method has a wide application prospect.

The present invention discloses a method for producing perillaldehyde and / or perillyl lactone, comprising: contacting a first component with a second component to produce perillaldehyde and / or perillyl lactone; wherein the first component is selected from components containing lysine-2-diol, and the second component is selected from enzymes or microorganisms or their cell lysates or enzyme extracts capable of converting lysine-2-diol into perillaldehyde and / or perillyl lactone.

The invention belongs to the technical field of sclareol extraction, and discloses a sclareol extraction device which comprises a separation barrel and a motor, a treatment cavity and a bottom cavity are formed in the separation barrel, a conveying assembly is fixedly arranged in the treatment cavity, and a buoyancy suction assembly is arranged on the outer side of the conveying assembly in a communicating mode. According to the invention, through the cooperation of the buoyancy suction assembly and the eduction pipe, in the process of mixing and extracting materials and an extracting agent, the conveying assembly and the buoyancy suction assembly are utilized to suck a large amount of floating solid materials and the extracting agent together into the conveying assembly along the position above the liquid level; the floating solid material is introduced and immersed into the extracting agent liquid again through the leading-out pipe after being led out upwards, on one hand, the mixing contact effect of the floating solid material and the extracting agent is improved, the problems of low contact area under floating and poor lifting efficiency are avoided, on the other hand, disturbance treatment of the mixed material liquid is realized, and a stirring mechanism does not need to be added; therefore, liquid disturbance and uniform mixing can be realized, and the comprehensive use effect is good.

An agent containing at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl)ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide is effective for masking an unpleasant smell and for making oral products in which an unpleasant smell is masked.

Variants of squalene hopene cyclase (SHC) are provided for enzymatically converting homofarnesoic acid to sclareolide, which can be non-enzymatically converted to ambrox.

To provide a masking agent for unpleasant odors derived from plants, in particular a grassy odor of soybean and an unpleasant odor derived from fats and oils of processed meat products (such as a hexanal odor), a method for masking unpleasant odors using the compound, and a method for producing an oral product in which unpleasant odors are masked.SOLUTION: The present invention provides an unpleasant-odor masking agent for oral products which contains at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl) ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide.SELECTED DRAWING: None

The present invention provides a clary sage oil and a preparation method thereof. Compared with the prior art, the present invention simultaneously extracts clary sage essential oil and separates sclareol using high-speed countercurrent chromatography. The sclareol obtained by high-speed countercurrent chromatography is then added to the clary sage essential oil to produce a new clary sage oil. This new clary sage oil has a high sclareol content, is effective and targeted, and significantly improves the quality of the essential oil, with a more pronounced amber-ambergris aroma and better smoking performance. Application in cigarettes can significantly improve the smoking experience of cigarettes.

This invention belongs to the field of biosynthesis technology, specifically relating to a strain for producing perillaldehyde, its construction method, and its application. Addressing the microbial synthesis problem of perillaldehyde, this invention mutates and optimizes the phosphotransketase in the NOG pathway, using a strain derived from nanoarchaemonas (…). Nanoarchaeota archaeon Site-directed mutagenesis was performed on the phosphoketolase NaXpk, and a perillaldehyde synthesis pathway was constructed in E. coli. The effects of NaXpk mutation on perillaldehyde yield were compared to identify the S472F and S472Y mutants. These mutants were then applied to the perillaldehyde production pathway, and the resulting production strains achieved perillaldehyde yields of 623.3 mg / L and 655.2 mg / L, respectively, demonstrating significant effectiveness.

To provide a technique for inhibiting 2,6-dimethylpyridine odor.SOLUTION: 2,6-dimethylpyridine odor is inhibited in food products and other targets by using sclareol, (3R / 3S)-3-sulfanylpentane-2-one, 2-methyl-3-furanthiol, magnolol, 3,5-dichlorophenol, tridecylic acid, propyl gallate, ethyl gallate, 1,5-pentanedial, phenethyl isothiocyanate, methyl heptadienone, β-elemene, capsaicin, 1-phenyl-1,3-butanedione, methyl isothiocyanate, methyl linolenate, ethyl isothiocyanate, 6-acetyl-1,1,2,4,4,7-hexamethyltetralin, 2,5-dimethyl-3-furanthiol, carvacrol, ethyl mercaptoacetate, pepper seed oil (PEPPER OIL BLACK), apigenin, 1-pentan-3-one, N-(4-hydroxy-3-methoxybenzyl)nonanamide, (E)-β-damascenone, 3-mercapto-1-hexanol, β-caryophyllene, or 2,6-dichlorobenzaldehyde.SELECTED DRAWING: None

The invention provides an application of a TRPV4 ion channel inhibitor in preparation of a medicine for preventing and / or treating psoriasis. Specifically, through screening of natural product monomer drugs, it is found that sclareol can inhibit a TRPV4 ion channel in a dose-dependent mode, application of sclareol in the field of psoriasis prevention and treatment is further developed, and a new strategy is provided for clinical treatment of psoriasis.

To provide a masking agent for unpleasant odors derived from plants, in particular a grassy odor of soybean and an unpleasant odor derived from fats and oils of processed meat products (such as a hexanal odor), a method for masking unpleasant odors using the compound, and a method for producing an oral product in which unpleasant odors are masked.SOLUTION: The present invention provides an unpleasant-odor masking agent for oral products which contains at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl) ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide.SELECTED DRAWING: None

The present invention relates to the technical field of sclareol preparation, and discloses a sclareol fermentation broth purification method, which comprises: centrifuging a sclareol fermentation broth at a rotation speed of 5000-15000 rpm for 15-30 min, removing an upper layer bacterial liquid, collecting lower layer thalli, and drying. The product I and the product II are respectively analyzed and measured by liquid chromatography, and the analysis result is that the content gt of sclareol in the product I; the yield is 97%, and the yield is gt; 70%; the content gt of the eulalcohol in the product; the yield is gt; 65%; the total yield of the product I and the product II can reach more than 90%, and the method has the advantages of few steps in the technological process, simplicity in operation, simplicity in used solvent, low toxicity, cyclic utilization and environmental friendliness. Meanwhile, the yield is high, the total recovery rate of sclareol in fermentation liquor can reach 90% or above, the production cost is greatly reduced, and industrialization of a process route for producing sclareol by using a synthetic biological means can be possibly realized.

The present application belongs to the field of biosynthesis, and particularly relates to a sclareol synthase and a yeast engineering bacterium for producing sclareol. In order to solve the problem of low yield of sclareol biosynthesis in the prior art, the sclareol synthase is mutated and optimized in the present application, a SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability existing in the expression of free plasmid in some Saccharomyces cerevisiae strains, the present application constructs a sclareol synthesis pathway in the yeast genome, strengthens the acetyl coenzyme A synthesis pathway, the MVA pathway and the sclareol synthesis pathway, and knocks out part of the transcriptional repressor to modify the chassis bacterium for synthesizing sclareol. The yield in a 5 L fermenter reaches 21.13 g / L g / L, which is greatly improved compared with the prior art.

The invention discloses a method for extracting sclareol from fermentation liquor. The method comprises the following operation steps: 1, treating the fermentation liquor; standing the fermentation liquor needing to be treated for 1-3 hours, putting the standing fermentation liquor into a centrifugal machine, and carrying out centrifugal treatment, so as to obtain mixed liquor of bacterial liquid and sclareol; 2, performing secondary treatment on the fermentation liquid; the centrifuged bacterial liquid and the mixed liquid are treated, the bacterial liquid is located on the upper layer of the mixed liquid, the bacterial liquid above the mixed liquid is removed, the mixed liquid is obtained, a cosolvent is added into the mixed liquid for mixing, and sclareol in the mixed liquid is dissolved by the mixed cosolvent; according to the method, the cosolvent is added into the fermentation liquor to re-dissolve the sclareol crystal part, then low-temperature crystal growing is conducted, the sclareol crystal part in the fermentation liquor is dissolved and then recrystallized into enlarged particles, the filtering difficulty is reduced, the product yield is guaranteed, and by means of subsequent drying, solvent extraction and crystallization, the yield of the sclareol is improved. The extraction process is simplified, and the product content is improved.

The invention relates to a method for extracting sclareol from yeast fermentation liquor, which comprises the following steps: 1) performing high-pressure homogenizing and crushing on the yeast fermentation liquor containing sclareol by using a high-pressure microjet homogenizer to obtain homogenized liquor; (2) heating the homogenized liquid to 80-105 DEG C, and then cooling and growing crystals to obtain flocculated liquid; (3) filtering the flocculated liquid by using a 50nm ceramic membrane to separate filtrate, so as to obtain a primary concentrated solution; (4) adding an organic solvent into the primary concentrated solution, and dissolving and crystallizing to obtain an extracting solution; (5) sequentially carrying out centrifugal separation, 1-micron fine filtration, 500D nanofiltration and reverse osmosis membrane concentration on the extracting solution to obtain a secondary concentrated solution; and (6) rectifying the secondary concentrated solution, cooling, crystallizing, and centrifugally separating to obtain sclareol crystals. The method disclosed by the invention is simple and convenient in extraction process and easy to realize industrial large-scale production. According to the method, the content of the obtained sclareol product is greater than 98%, and the pure yield of sclareol in the fermentation liquor reaches 90% or above.

The invention provides an extraction and purification method of sclareolide. The extraction and purification method comprises the following steps: mixing a sclareolide raw material with an organic solvent, and collecting an organic phase to obtain a sclareolide mixed solution; concentrating the sclareolide mixed solution, and carrying out cooling crystallization to obtain a sclareolide coarse crystal; and carrying out recrystallization or crystal washing on the sclareolide coarse crystal to obtain the sclareolide, the sclareolide raw materials comprise the sclareolide and sclareol. The method can solve the problem of complex process for extracting and purifying sclareolide in the prior art, and is suitable for the field of preparation of sclareolide.

The invention discloses a method for separating and detecting sclareol, sclareolide and sclarediol, and relates to the technical field of substance separation and detection. The separation and detection method comprises the following steps: preparing a mixed standard solution of sclareol, sclareolide and sclarediol; detecting the mixed standard substance solution by high performance liquid chromatography to obtain a standard curve and a linear regression equation corresponding to sclareol, sclareolide and sclarediol; and carrying out separation detection on sclareol, sclareolide and sclarediol in the catalytic solution to be detected by utilizing a high performance liquid chromatography. According to the method disclosed by the invention, qualitative analysis, quantitative detection and component separation of sclareol, sclareolide and sclarediol in a catalytic solution to be detected catalyzed by fungi ATCC 20624 are realized by utilizing a high performance liquid chromatography, and the quantitative detection accuracy is close to a detection result of a gas chromatography.

The invention discloses cryptococcus albidus and application of the cryptococcus albidus in synthesis of norambrox ether, and belongs to the technical field of bioengineering. The preservation number of the cryptococcus albidus JMIC881 is CCTCC (China Center for Type Culture Collection) NO: M 20251916, the preservation date is September 1, 2025, and the preservation unit is the China Center for Type Culture Collection. The strain JMIC881 is separated from clary sage flowers, and is a microbial strain obtained by natural breeding and ARTP mutation breeding of wild cryptococcus light white yeast. The invention also provides a method for producing norambrox ether by using sclareol as a substrate through fermentation by using the strain JMIC881, and the method has the advantages of mild reaction conditions, no chemical pollutant emission, high product selectivity, low energy consumption and the like, solves the environmental problem caused by the traditional chemical synthesis method, reduces the equipment cost and energy consumption of industrial production, and is suitable for industrial production. And a new path is provided for green preparation of the ambrogyl ether.

The purpose of the present invention is to provide a food composition and a masking agent capable of suppressing unpleasant odor.　Provided is a food composition or a masking agent for suppressing unpleasant odor that contains at least one type selected from the group consisting of sclareolide, sclareol, E-β-damascone, and 2-thiophenethiol, wherein: if sclareolide is contained, the sclareolide content is 0.000001-100 ppm by weight of the entire composition or the entire agent; if sclareol is contained, the sclareol content is 0.01-1,000,000 ppm by weight of the entire composition or the entire agent; if E-β-damascone is contained, the E-β-damascone content is 0.000001-10 ppm by weight of the entire composition or the entire agent; and if 2-thiophenethiol is contained, the 2-thiophenethiol content is 0.0001-10,000 ppm by weight of the entire composition or the entire agent.