34 results about "Sclareol" patented technology

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The invention belongs to the technical field of biosynthesis, and particularly relates to a strain for producing sclareol as well as a construction method and application of the strain. In order to solve the problem of microbial synthesis of sclareol, phosphoketolase in an NOG pathway is mutated and optimized, phosphoketolase NaXpk from nanoarchaea archaeon is subjected to site-directed mutagenesis, a sclareol synthesis pathway is constructed in an escherichia coli host, the influence of NaXpk before and after mutation on the yield of sclareol is compared, and the yield of sclareol is improved. The S472F mutant and the S472Y mutant are determined, the S472F mutant and the S472Y mutant are applied to a sclareol production path, the sclareol yield of the finally obtained production strains can reach 623.3 mg / L and 655.2 mg / L, and the effect is remarkable.

The present invention relates to the technical field of aromatherapy, and specifically discloses a compound essential oil for alleviating anxiety, its preparation method and application. A compound essential oil for alleviating anxiety comprises raw materials in the following parts by mass: 50 - 65 parts of sweet almond oil, 12 - 16 parts of French lavender oil, 0.5 - 1.2 parts of Xinjiang lavender oil, 6 - 9 parts of cypress oil essential oil, 1 - 3 parts of Virginia cypress oil, 0.1 - 0.3 parts of complete ylang-ylang oil, 0.7 - 1.3 parts of Xinjiang sclareol oil, 2 - 4 parts of bergamot oil, and 2 - 4 parts of eucalyptus oil. The preparation method of the compound essential oil for alleviating anxiety is to mix the raw materials to obtain the compound essential oil. The present application also provides an application of the compound essential oil for alleviating anxiety in aromatherapy products. The compound essential oil for alleviating anxiety in the present application has obvious effects of alleviating anxiety emotions and improving sleep quality.

The invention provides an engineering bacterium and a method for synthesizing sclarediol. Wherein the engineering bacterium comprises a sclareone synthase gene and a Bayer-Villiger monooxygenase gene, and the sclareone synthase comprises: (a1) a protein having any sequence in SEQ ID NOs: 1-25, or (a2) a protein having any sequence in SEQ ID NOs: 2-25, or (a3) a protein having any sequence in SEQ ID NOs: 2-25; (a2) protein which has more than 70% of homology with the amino acid sequence limited in (a1) and has sclareone synthase activity; the Bayer-Villiger monooxygenase comprises: (b1) a protein having any one sequence in SEQ ID NOs: 26-50, or (b2) a protein having any one sequence in SEQ ID NOs: 26-50; (b2) is protein which has more than 70% of homology with the amino acid sequence limited in (b1) and has Bayer-Villiger monooxygenase activity. The method can solve the problem of difficulty in efficient biosynthesis of sclarediol in the prior art, and is suitable for the field of synthetic biology.

The present invention belongs to the field of microbial metabolic engineering and synthetic biology technology applications, and particularly relates to a Pichia pastoris engineered strain that produces high sclareol production, as well as its construction method and application. A sclareol biosynthesis pathway is constructed in a host strain, and the intracellular mevalonate metabolic pathway and the central metabolic pathway are optimized, thereby obtaining an engineered bacterial strain A; the host strain is Pichia pastoris; or, metabolic regulatory factors are overexpressed or knocked out in the above-obtained engineered bacterial strain A, thereby obtaining an engineered bacterial strain B; or, in the above-obtained engineered bacterial strain B, a cell compartmentalization strategy is used to target the synthesis pathway to the peroxisome and optimize it, thereby obtaining an engineered bacterial strain C. The present invention provides a Pichia pastoris chassis cell for synthesizing the diterpenoid compound sclareol and an engineered strain for synthesizing sclareol; by introducing and optimizing the expression of the sclareol synthesis pathway, the sclareol yields in shake flask batch fermentation and bioreactor batch fed-batch fermentation reach 631.6 mg / L and 10.5 g / L, respectively.

The invention provides a preparation method of sclareolide and a catalyst, and belongs to the technical field of organic catalysis. The method comprises the following steps: dissolving sclareol by using a first solvent, adding a catalyst, adjusting the pH value of the solution under microwave conditions, dropwise adding hydrogen peroxide, heating and stirring for reaction, separating the catalyst, reusing, extracting the product by using a second solvent, and heating and refluxing to obtain sclareolide; the catalyst is imidazolyl ionic liquid / polyethylene glycol / maleic acid modified graphene oxide loaded with vacancy heteropolyacid salt. According to the preparation method of sclareolide, microwave-assisted reaction is adopted, so that the product yield of sclareolide is greatly improved. According to the invention, two mutually compatible reaction systems of alcohol and water are adopted, so that the environmental protection property of the process is greatly improved, and meanwhile, under the action of the catalyst, the method has a phase catalytic transfer effect, the reaction efficiency is improved, the reaction condition is mild, the product yield is high, and the method has a wide application prospect.

The present invention discloses a method for producing perillaldehyde and / or perillyl lactone, comprising: contacting a first component with a second component to produce perillaldehyde and / or perillyl lactone; wherein the first component is selected from components containing lysine-2-diol, and the second component is selected from enzymes or microorganisms or their cell lysates or enzyme extracts capable of converting lysine-2-diol into perillaldehyde and / or perillyl lactone.

The invention belongs to the technical field of sclareol extraction, and discloses a sclareol extraction device which comprises a separation barrel and a motor, a treatment cavity and a bottom cavity are formed in the separation barrel, a conveying assembly is fixedly arranged in the treatment cavity, and a buoyancy suction assembly is arranged on the outer side of the conveying assembly in a communicating mode. According to the invention, through the cooperation of the buoyancy suction assembly and the eduction pipe, in the process of mixing and extracting materials and an extracting agent, the conveying assembly and the buoyancy suction assembly are utilized to suck a large amount of floating solid materials and the extracting agent together into the conveying assembly along the position above the liquid level; the floating solid material is introduced and immersed into the extracting agent liquid again through the leading-out pipe after being led out upwards, on one hand, the mixing contact effect of the floating solid material and the extracting agent is improved, the problems of low contact area under floating and poor lifting efficiency are avoided, on the other hand, disturbance treatment of the mixed material liquid is realized, and a stirring mechanism does not need to be added; therefore, liquid disturbance and uniform mixing can be realized, and the comprehensive use effect is good.

An agent containing at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl)ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide is effective for masking an unpleasant smell and for making oral products in which an unpleasant smell is masked.

Variants of squalene hopene cyclase (SHC) are provided for enzymatically converting homofarnesoic acid to sclareolide, which can be non-enzymatically converted to ambrox.

To provide a masking agent for unpleasant odors derived from plants, in particular a grassy odor of soybean and an unpleasant odor derived from fats and oils of processed meat products (such as a hexanal odor), a method for masking unpleasant odors using the compound, and a method for producing an oral product in which unpleasant odors are masked.SOLUTION: The present invention provides an unpleasant-odor masking agent for oral products which contains at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl) ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide.SELECTED DRAWING: None

The present invention provides a clary sage oil and a preparation method thereof. Compared with the prior art, the present invention simultaneously extracts clary sage essential oil and separates sclareol using high-speed countercurrent chromatography. The sclareol obtained by high-speed countercurrent chromatography is then added to the clary sage essential oil to produce a new clary sage oil. This new clary sage oil has a high sclareol content, is effective and targeted, and significantly improves the quality of the essential oil, with a more pronounced amber-ambergris aroma and better smoking performance. Application in cigarettes can significantly improve the smoking experience of cigarettes.

This invention belongs to the field of biosynthesis technology, specifically relating to a strain for producing perillaldehyde, its construction method, and its application. Addressing the microbial synthesis problem of perillaldehyde, this invention mutates and optimizes the phosphotransketase in the NOG pathway, using a strain derived from nanoarchaemonas (…). Nanoarchaeota archaeon Site-directed mutagenesis was performed on the phosphoketolase NaXpk, and a perillaldehyde synthesis pathway was constructed in E. coli. The effects of NaXpk mutation on perillaldehyde yield were compared to identify the S472F and S472Y mutants. These mutants were then applied to the perillaldehyde production pathway, and the resulting production strains achieved perillaldehyde yields of 623.3 mg / L and 655.2 mg / L, respectively, demonstrating significant effectiveness.

The invention provides an application of a TRPV4 ion channel inhibitor in preparation of a medicine for preventing and / or treating psoriasis. Specifically, through screening of natural product monomer drugs, it is found that sclareol can inhibit a TRPV4 ion channel in a dose-dependent mode, application of sclareol in the field of psoriasis prevention and treatment is further developed, and a new strategy is provided for clinical treatment of psoriasis.

To provide a masking agent for unpleasant odors derived from plants, in particular a grassy odor of soybean and an unpleasant odor derived from fats and oils of processed meat products (such as a hexanal odor), a method for masking unpleasant odors using the compound, and a method for producing an oral product in which unpleasant odors are masked.SOLUTION: The present invention provides an unpleasant-odor masking agent for oral products which contains at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl) ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide.SELECTED DRAWING: None

The present invention relates to the technical field of sclareol preparation, and discloses a sclareol fermentation broth purification method, which comprises: centrifuging a sclareol fermentation broth at a rotation speed of 5000-15000 rpm for 15-30 min, removing an upper layer bacterial liquid, collecting lower layer thalli, and drying. The product I and the product II are respectively analyzed and measured by liquid chromatography, and the analysis result is that the content gt of sclareol in the product I; the yield is 97%, and the yield is gt; 70%; the content gt of the eulalcohol in the product; the yield is gt; 65%; the total yield of the product I and the product II can reach more than 90%, and the method has the advantages of few steps in the technological process, simplicity in operation, simplicity in used solvent, low toxicity, cyclic utilization and environmental friendliness. Meanwhile, the yield is high, the total recovery rate of sclareol in fermentation liquor can reach 90% or above, the production cost is greatly reduced, and industrialization of a process route for producing sclareol by using a synthetic biological means can be possibly realized.

The present application belongs to the field of biosynthesis, and particularly relates to a sclareol synthase and a yeast engineering bacterium for producing sclareol. In order to solve the problem of low yield of sclareol biosynthesis in the prior art, the sclareol synthase is mutated and optimized in the present application, a SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability existing in the expression of free plasmid in some Saccharomyces cerevisiae strains, the present application constructs a sclareol synthesis pathway in the yeast genome, strengthens the acetyl coenzyme A synthesis pathway, the MVA pathway and the sclareol synthesis pathway, and knocks out part of the transcriptional repressor to modify the chassis bacterium for synthesizing sclareol. The yield in a 5 L fermenter reaches 21.13 g / L g / L, which is greatly improved compared with the prior art.

The invention discloses a method for extracting sclareol from fermentation liquor. The method comprises the following operation steps: 1, treating the fermentation liquor; standing the fermentation liquor needing to be treated for 1-3 hours, putting the standing fermentation liquor into a centrifugal machine, and carrying out centrifugal treatment, so as to obtain mixed liquor of bacterial liquid and sclareol; 2, performing secondary treatment on the fermentation liquid; the centrifuged bacterial liquid and the mixed liquid are treated, the bacterial liquid is located on the upper layer of the mixed liquid, the bacterial liquid above the mixed liquid is removed, the mixed liquid is obtained, a cosolvent is added into the mixed liquid for mixing, and sclareol in the mixed liquid is dissolved by the mixed cosolvent; according to the method, the cosolvent is added into the fermentation liquor to re-dissolve the sclareol crystal part, then low-temperature crystal growing is conducted, the sclareol crystal part in the fermentation liquor is dissolved and then recrystallized into enlarged particles, the filtering difficulty is reduced, the product yield is guaranteed, and by means of subsequent drying, solvent extraction and crystallization, the yield of the sclareol is improved. The extraction process is simplified, and the product content is improved.

The invention provides an extraction and purification method of sclareolide. The extraction and purification method comprises the following steps: mixing a sclareolide raw material with an organic solvent, and collecting an organic phase to obtain a sclareolide mixed solution; concentrating the sclareolide mixed solution, and carrying out cooling crystallization to obtain a sclareolide coarse crystal; and carrying out recrystallization or crystal washing on the sclareolide coarse crystal to obtain the sclareolide, the sclareolide raw materials comprise the sclareolide and sclareol. The method can solve the problem of complex process for extracting and purifying sclareolide in the prior art, and is suitable for the field of preparation of sclareolide.

The invention discloses cryptococcus albidus and application of the cryptococcus albidus in synthesis of norambrox ether, and belongs to the technical field of bioengineering. The preservation number of the cryptococcus albidus JMIC881 is CCTCC (China Center for Type Culture Collection) NO: M 20251916, the preservation date is September 1, 2025, and the preservation unit is the China Center for Type Culture Collection. The strain JMIC881 is separated from clary sage flowers, and is a microbial strain obtained by natural breeding and ARTP mutation breeding of wild cryptococcus light white yeast. The invention also provides a method for producing norambrox ether by using sclareol as a substrate through fermentation by using the strain JMIC881, and the method has the advantages of mild reaction conditions, no chemical pollutant emission, high product selectivity, low energy consumption and the like, solves the environmental problem caused by the traditional chemical synthesis method, reduces the equipment cost and energy consumption of industrial production, and is suitable for industrial production. And a new path is provided for green preparation of the ambrogyl ether.

This invention provides a recombinant lipolytic yeast strain with high perillaldehyde production, its preparation method, and its applications. This recombinant lipolytic yeast strain can produce perillaldehyde with high yield and efficiency, effectively solving the problem of low yield in existing recombinant Yersinia lipolytic yeast fermentation for perillaldehyde production. Furthermore, the batch-to-batch stability of perillaldehyde production using this recombinant lipolytic yeast strain is good, meeting the requirements for product stability in large-scale industrial production. Therefore, the recombinant lipolytic yeast strain of this application has high production efficiency in perillaldehyde production, thus showing good prospects for industrial application.

The invention belongs to the technical field of microorganisms, and relates to bacillus for redried tobacco, a culture method and application in tobacco fermentation, the bacillus strain S-209 has the preservation number of CGMCC No.35010, and is preserved in China General Microbiological Culture Collection Center on June 26, 2025; the bacillus strain S-209 disclosed by the invention can be used as a fermentation strain to act on redried tobacco leaves to play a role; according to the bacillus disclosed by the invention, the content of dihydroactinidiolide, 9-hydroxy-4, 7-megabean diene-3-ketone, 6-ethyl-5, 6-dihydro-2H-pyradine-2-ketone, ethyl palmitate, 4-cyclopentene-1, 2, 4-triazole-3-ketone, 4-cyclopentene-1, 2, 3-triazole-3-ketone, 4-cyclopentene-1, 2, 3-triazole-3-ketone, 4-cyclopentene-1, 2, 3-triazole-3-ketone, the contents of 1, 3-diketone, 4-hydroxy-beta-dihydrodamascone, farnesyl acetone and geranyl acetone are increased, and aroma components such as sclareol and geranyl aromatic alcohol are added, so that the aroma components in smoke are increased, and the smoking quality is improved.

The invention belongs to the technical field of chemical engineering, and relates to a microbial catalyst, in particular to a method for increasing the content of sclareol in aromatic tobacco. The method comprises the following specific steps: after sorting raw tobaccos, applying a microbial catalyst to the surface of the selected raw tobaccos according to a set tobacco leaf mass ratio; the microbial catalyst is bacillus ST-2 (Bacillus sp.) which is preserved in the China General Microbiological Culture Collection Center on May 17, 2024, and the preservation number of the bacillus ST-2 is CGMCC NO: 30672; and adjusting the moisture content of the tobacco leaves to a set amount, and standing for a set time under the conditions of set temperature and humidity. According to the technical scheme, the microbial catalyst is used for degrading cis-abienol, and the content of sclareol in the aromatic tobacco leaves sprayed with the microbial catalyst is remarkably increased compared with the content of sclareol in the aromatic tobacco leaves not sprayed with the microbial catalyst.

The present disclosure relates to the field of personal care and more particularly to a method for improving scalp condition and hair growth, which comprises applying to the scalp a composition comprising an active agent selected among sclareol, sclareol-like, sclareolide or sclareolide-like and a physiologically acceptable carrier

The invention discloses a high-yield sclareol recombinant strain as well as a construction method and application thereof, and belongs to the technical field of microbial metabolism engineering. The invention provides recombinant yarrowia lipolytica SC37 with high yield of sclareol, which is characterized in that a sclareol biosynthesis path is firstly constructed, then acetyl coenzyme A in peroxidase is guided to a mevalonic acid path, and then a precursor acetyl coenzyme A and a cofactor NADPH are introduced by optimizing an intracellular mevalonic acid metabolism path to obtain the recombinant strain SC37. The invention provides a recombinant strain SC37 which takes waste oil as a substrate and is high in sclareol yield, and by introducing and optimizing the expression of a sclareol synthesis path, the sclareol yields in shake flask batch fed-batch fermentation and bioreactor batch fed-batch fermentation respectively reach 2.5 g / L and 20.18 g / L. The recombinant strain SC37 has important values for reducing environmental pollution and increasing waste oil economy, and has a good application prospect.

To provide a masking agent for unpleasant odors derived from plants, in particular a grassy odor of soybean and an unpleasant odor derived from fats and oils of processed meat products (such as a hexanal odor), a method for masking unpleasant odors using the compound, and a method for producing an oral product in which unpleasant odors are masked.SOLUTION: The present invention provides an unpleasant-odor masking agent for oral products which contains at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl) ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide.SELECTED DRAWING: None

The invention belongs to the technical field of strain fermentation, and particularly relates to a strain fermentation method for synthesizing sclareol, which comprises the following steps: step 1, constructing a strain; step 2, adding hosts; 3, the Yarrowia lipolytica is recombined; step 4, fermentation of sclareol; and 5, fermenting other flower buds to produce sclareol. In the invention, the construction method relates to expression of plant-derived sclareol to synthesize two key enzymes, i.e., lysodanediol pyrophosphate synthase and sclareol synthase, and overexpression of a combination of one or more enzyme genes endogenous by yeast. Comprising a mevalonic acid pathway key enzyme-hydroxymethyl coenzyme A reductase and an isopentenyl pyrophosphate pathway two-enzyme-farnesyl pyrophosphate synthase and dibovine pyrophosphate synthase, sclareol can be efficiently produced, and compared with a method adopting tobacco flower buds as a unique raw material, no additional nutritional ingredients are added, and the production cost is reduced. The sclareol can be synthesized in a green and efficient manner only by fermenting the tobacco flower buds with the edible yeast as a leavening agent.

The invention belongs to the technical field of sclareol production, and particularly relates to a sclareol production fermentation tail gas treatment device which comprises a fermentation kettle body, a feed crushing mechanism, a tail gas purification filter element and a liquid box. According to the invention, the kettle cover is closed during fermentation, generated tail gas enters the gas inlet pipe, and when the pressure in the fermentation kettle body is gradually increased, the sealing plug moves downwards, so that the pressure spring is stretched until the sealing plug moves to the lower part of the gas release port, and the gas release port communicates the upper and lower areas of the sealing partition plate; the tail gas can automatically enter an area below the sealing partition plate through the gas inlet pipe and the gas outlet, and the sealing plug can be in contact with the touch switch during the period, so that the infusion pump is started and the absorption liquid is sprayed into the fermentation kettle body through the pipeline and the atomizing nozzle, and the tail gas and the absorption liquid are fully mixed; and the mixed liquid is treated through the filter screen and the tail gas purification filter element, so that the treatment quality is guaranteed, and the treated tail gas can be normally discharged.

The invention relates to the technical field of condensing equipment, in particular to sclareol fermentation liquor extracting and condensing equipment which comprises a cooling tower, a plurality of condensing pipes are fixedly arranged in the cooling tower, a discharge mechanism is movably arranged in each condensing pipe, and a blocking mechanism is arranged in each discharge mechanism. By arranging the flow guide groove and the liquid inlet, the condensed sclareol fermentation liquor flows into the liquid accumulation sliding cylinder, and due to the insertion effect of the plug I and the discharge port, the sclareol fermentation liquor in the liquid accumulation sliding cylinder cannot be discharged through the discharge port, so that the sclareol fermentation liquor is continuously accumulated in the liquid accumulation sliding cylinder; when the sclareol fermentation liquor is completely condensed, part of the high-temperature gaseous sclareol fermentation liquor is not completely condensed and enters the liquid accumulation sliding cylinder, so that the sclareol fermentation liquor can be cooled again in the process of accumulating the sclareol fermentation liquor through the liquid accumulation sliding cylinder, and the effect of complete liquefaction is achieved.

The invention belongs to the field of biotechnology and microbial fermentation engineering, and particularly relates to a cryptococcus albidus strain and application thereof in production of sclareolide. A mutant strain with the most excellent performance is screened out through systematic wild strain screening, natural breeding, ARTP mutation breeding and other technical means, the mutant strain is named as Cryptococcus albidus FM1120, the preservation number is CCTCC NO: M 20251915, the preservation date is September 1, 2025, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915. The preservation unit is China Center for Type Culture Collection. The invention also provides an application of the strain FM1120 in the production of sclareolide, sclareol is taken as a substrate, the conversion rate of sclareol reaches more than 90% through fermentation, the fermentation liquor has the advantages of few impurities, easiness in separation and purification of sclareolide, high purity and the like, and a new path is provided for the green preparation of sclareolide.