10 results about "Sclareol" patented technology

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The invention provides an engineering bacterium and a method for synthesizing sclarediol. Wherein the engineering bacterium comprises a sclareone synthase gene and a Bayer-Villiger monooxygenase gene, and the sclareone synthase comprises: (a1) a protein having any sequence in SEQ ID NOs: 1-25, or (a2) a protein having any sequence in SEQ ID NOs: 2-25, or (a3) a protein having any sequence in SEQ ID NOs: 2-25; (a2) protein which has more than 70% of homology with the amino acid sequence limited in (a1) and has sclareone synthase activity; the Bayer-Villiger monooxygenase comprises: (b1) a protein having any one sequence in SEQ ID NOs: 26-50, or (b2) a protein having any one sequence in SEQ ID NOs: 26-50; (b2) is protein which has more than 70% of homology with the amino acid sequence limited in (b1) and has Bayer-Villiger monooxygenase activity. The method can solve the problem of difficulty in efficient biosynthesis of sclarediol in the prior art, and is suitable for the field of synthetic biology.

The invention provides a preparation method of sclareolide and a catalyst, and belongs to the technical field of organic catalysis. The method comprises the following steps: dissolving sclareol by using a first solvent, adding a catalyst, adjusting the pH value of the solution under microwave conditions, dropwise adding hydrogen peroxide, heating and stirring for reaction, separating the catalyst, reusing, extracting the product by using a second solvent, and heating and refluxing to obtain sclareolide; the catalyst is imidazolyl ionic liquid / polyethylene glycol / maleic acid modified graphene oxide loaded with vacancy heteropolyacid salt. According to the preparation method of sclareolide, microwave-assisted reaction is adopted, so that the product yield of sclareolide is greatly improved. According to the invention, two mutually compatible reaction systems of alcohol and water are adopted, so that the environmental protection property of the process is greatly improved, and meanwhile, under the action of the catalyst, the method has a phase catalytic transfer effect, the reaction efficiency is improved, the reaction condition is mild, the product yield is high, and the method has a wide application prospect.

The present invention discloses a method for producing perillaldehyde and / or perillyl lactone, comprising: contacting a first component with a second component to produce perillaldehyde and / or perillyl lactone; wherein the first component is selected from components containing lysine-2-diol, and the second component is selected from enzymes or microorganisms or their cell lysates or enzyme extracts capable of converting lysine-2-diol into perillaldehyde and / or perillyl lactone.

An agent containing at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl)ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide is effective for masking an unpleasant smell and for making oral products in which an unpleasant smell is masked.

The present application belongs to the field of biosynthesis, and particularly relates to a sclareol synthase and a yeast engineering bacterium for producing sclareol. In order to solve the problem of low yield of sclareol biosynthesis in the prior art, the sclareol synthase is mutated and optimized in the present application, a SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability existing in the expression of free plasmid in some Saccharomyces cerevisiae strains, the present application constructs a sclareol synthesis pathway in the yeast genome, strengthens the acetyl coenzyme A synthesis pathway, the MVA pathway and the sclareol synthesis pathway, and knocks out part of the transcriptional repressor to modify the chassis bacterium for synthesizing sclareol. The yield in a 5 L fermenter reaches 21.13 g / L g / L, which is greatly improved compared with the prior art.

This invention provides a recombinant lipolytic yeast strain with high perillaldehyde production, its preparation method, and its applications. This recombinant lipolytic yeast strain can produce perillaldehyde with high yield and efficiency, effectively solving the problem of low yield in existing recombinant Yersinia lipolytic yeast fermentation for perillaldehyde production. Furthermore, the batch-to-batch stability of perillaldehyde production using this recombinant lipolytic yeast strain is good, meeting the requirements for product stability in large-scale industrial production. Therefore, the recombinant lipolytic yeast strain of this application has high production efficiency in perillaldehyde production, thus showing good prospects for industrial application.

The invention belongs to the technical field of microorganisms, and relates to bacillus for redried tobacco, a culture method and application in tobacco fermentation, the bacillus strain S-209 has the preservation number of CGMCC No.35010, and is preserved in China General Microbiological Culture Collection Center on June 26, 2025; the bacillus strain S-209 disclosed by the invention can be used as a fermentation strain to act on redried tobacco leaves to play a role; according to the bacillus disclosed by the invention, the content of dihydroactinidiolide, 9-hydroxy-4, 7-megabean diene-3-ketone, 6-ethyl-5, 6-dihydro-2H-pyradine-2-ketone, ethyl palmitate, 4-cyclopentene-1, 2, 4-triazole-3-ketone, 4-cyclopentene-1, 2, 3-triazole-3-ketone, 4-cyclopentene-1, 2, 3-triazole-3-ketone, 4-cyclopentene-1, 2, 3-triazole-3-ketone, the contents of 1, 3-diketone, 4-hydroxy-beta-dihydrodamascone, farnesyl acetone and geranyl acetone are increased, and aroma components such as sclareol and geranyl aromatic alcohol are added, so that the aroma components in smoke are increased, and the smoking quality is improved.

The invention belongs to the field of biotechnology and microbial fermentation engineering, and particularly relates to a cryptococcus albidus strain and application thereof in production of sclareolide. A mutant strain with the most excellent performance is screened out through systematic wild strain screening, natural breeding, ARTP mutation breeding and other technical means, the mutant strain is named as Cryptococcus albidus FM1120, the preservation number is CCTCC NO: M 20251915, the preservation date is September 1, 2025, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915. The preservation unit is China Center for Type Culture Collection. The invention also provides an application of the strain FM1120 in the production of sclareolide, sclareol is taken as a substrate, the conversion rate of sclareol reaches more than 90% through fermentation, the fermentation liquor has the advantages of few impurities, easiness in separation and purification of sclareolide, high purity and the like, and a new path is provided for the green preparation of sclareolide.

The present application relates to a kind of biosynthesis methods of sclareolide, comprising the following steps: 1) in the fermentation process, every interval one sampling period t1, the content of sclareol in fermentation liquor is sampled and detected, and according to the content of sclareol in fermentation liquor detection value, the feeding speed of sclareol is adjusted;2) the volatilization amount of sclareol in fermentation tail gas is continuously detected, every interval one metering period t2, the volatilization total amount of sclareol is counted once, and the total amount of sclareol feeding in metering period is counted, the ratio of the volatilization total amount of sclareol and the total amount of sclareol feeding is calculated;3) when the ratio is less than threshold value, increase the feeding speed of sclareol.The present application judges the consumption variation of sclareol by continuously monitoring the ratio of the volatilization amount of sclareol in fermentation tail gas and the feeding amount of sclareol, can improve the timeliness of the concentration control of sclareol in fermentation liquor.The conversion rate and yield of biosynthesis sclareolide can be further improved by using the method.